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| 1 | 鸡源产气荚膜梭菌重组酶聚合酶扩增检测方法的建立显示文摘为建立鸡源产气荚膜梭菌(Clostridium perfringens)的快速诊断方法,本研究以产气荚膜梭菌α-毒素的编码基因plc为基础设计、合成引物和探针,并以real-time PCR的方法进行验证,建立了鸡源产气荚膜梭菌的重组酶聚合酶扩增(RPA)检测方法。该方法可在38℃、25 min内对产气荚膜梭菌的α-毒素编码序列进行快速特异性扩增,最低检测限量可达1×10^1copies/μL,灵敏度良好。以此方法检测实验室保存的临床样本,其检测结果与real-time PCR的检测结果一致。上述结果表明,本研究建立的real-time RPA方法在检测鸡源产气荚膜梭菌时具有操作简单、快速灵敏的特点,适用于鸡源产气荚膜梭菌的临床检测。 | 许笑 王艳华 M Umar Zafar Khan 王恒 蔡建平 | 2019 | 中国兽医科学2019，49，12： | 6 |
| 2 | Pattern of stroke in type 2 diabetic subjects versus non diabetic subjects显示文摘 | Zafar A Shahid SK Siddiqui M | 2007 | J Ayub Med Coll Abbottabad2007，19，4： | 1 |
| 3 | Mobilisation into cotton and spread of a recombinant cotton leaf curl disease satellite显示文摘 | Amin I Mansoor S Amrao L Hussain M Irum S Zafar Y Bull SE Briddon RW | | 0，，10： | 1 |
| 4 | Changing trends in fre- quency and antimicrobial susceptibility of pathogens cau- sing catheter related infections in children显示文摘 | Qasim M Zafar A Ejaz H | 2013 | African J Microbiol Res2013，5，7： | 1 |
| 5 | Anxiety is a better pre- dictor of platelet reactivity in coronaryartery disease patients than depression 显示文摘 | Zafar MU Paz-Yepes M Shimbo D | 2010 | Eur Heart J2010，31，13： | 1 |
| 6 | Insulin effects on bioavailability of soy isoflavones and their calcium absorption enhancing ability显示文摘 | ZAFAR T A WEAVER C M JONES K | 2004 | J Agric Food Chem2004，52，10： | 1 |
| 7 | Fluorodeoxyglueose posi- tron emission tomography studies in diagnosis 'and staging of clinically organ-confined prostate cancer显示文摘 | Liu I J Zafar M B Lai Y H | 2001 | Urology2001，57，1： | 1 |
| 8 | Beneficial route of low-grade phosphate rocks for fertilizer production显示文摘 | Zafar Z I Anwar M M Pritchard D W | 1995 | Ind Eng Chem Res1995，34，： | 1 |
| 9 | Selective leaching kinetics and upgrading of low-grade calcareous phosphate rock in succinic acid显示文摘 | ASHRAF M ZAFAR Z I ANSARI T M | 2005 | Hydrometallurgy2005，80，4： | 1 |
| 10 | The use of fibrous network of matured dried fruit of luffa aegyptica as immobilizing agent显示文摘 | Iqbal M Zafar S I | 1993 | Biotechnology Techniques1993，7，1： | 1 |
| 11 | Effect of pupillary dilation on retinal nerve fiber layer thickness measurements using optical coherence tomography显示文摘 | Zafar S Gurses-Ozden R Vessani R Makornwattana M Liebmann JM Tello C | 2004 | J Glaucoma2004，13，1： | 1 |
| 12 | Age,growth,mortal-ity and population structure of the oyster,Crassostrea madrasensis,in the Moheskhali Channel(southeastern coast of Bangladesh)显示文摘 | Amin S M N Zafar M Halim A | | 0，，： | 1 |
| 13 | Nitrogen is a Deep Acceptor in ZnO显示文摘 | Tarun M C Zafar I M McCluskey D M | 2011 | AIP Advances2011，，02： | 1 |
| 14 | Tryptophan repressor-binding proteins from escherichia coli and archaeoglobus fulgidus as new catalysts for 1,4-dihydronicotinamide adenine dinucleotide-dependent amperometric biosensors and biofuel cells显示文摘 | ZAFAR M N TASCA F GORTON L | 2009 | Anal Chem2009，81，10： | 1 |
| 15 | Cell-specific expression of estrogen receptor in the human pituitary and its adenomas显示文摘 | Ezzat S Ramyar L | 1995 | J Clin Endocrinol Metab1995，80，12： | 1 |
| 16 | FTIR spectrophotometry, kinetics and adsorption isotherms modeling, ion exchange, and EDX analysis for understanding the mechanism of Cd2 + and Pb2 + removal by mango peel waste 显示文摘 | Iqbal M Saeed A Zafar S I | 2009 | Journal of Hazardous Materials2009，164，： | 1 |
| 17 | 口服维生素C对人眼房水中抗坏血酸盐浓度的影响(英文)显示文摘AIM: To study oral administration of vitamin C onhuman aqueous humour ascorbate concentration.METHODS: High performance liquid chromatography(HPLC) coupled with electrochemical detector (ECD)was used. The effect of oral administraion of variousdoses of ascorbic acid, 0 (control), 1.0, 1.5., 2.0,3.0, and 5.0 g, on its concentration in aqueoushumour, obtained from volunteer cataract patients wasstudied. RESULTS: The concentration of ascorbicacid in aqueous humour of control group | Zafar IQBAL John M MIDGLEY David G WATSON Stilianous D KARDITSAS Gordon N DUTTON William S WILSON | 1999 | 中国药理学报1999，20，10： | 1 |
| 18 | FTIR spectrophotometry,kinetics and adsorption isotherms modeling,ion exchange,and EDX analysis for understanding the mechanism of Cd2+ and Pb2 + removal by mango peel waste显示文摘 | IQBAL M SAEED A ZAFAR S I | 2009 | Journal of Hazardous Materials2009，164，1： | 1 |
| 19 | A General Class of Contractions: d-Contractions 显示文摘 | Akram M Zafar A A Siddiqui A A | 2008 | Novi Sad J Math2008，38，1： | 1 |
| 20 | Functionalizafion of carbon nanotubes with amines and enzymes显示文摘 | Wang Y B Zafar I Sanjay V M | 2005 | Chemical Physics Letters2005，402，： | 1 |
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