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| 1 | Internet of Things to network smart devices for ecosystem monitoring显示文摘Smart, real-time, low-cost, and distributed ecosystem monitoring is essential for understanding and managing rapidly changing ecosystems. However, new techniques in the big data era have rarely been introduced into operational ecosystem monitoring, particularly for fragile ecosystems in remote areas.We introduce the Internet of Things(IoT) techniques to establish a prototype ecosystem monitoring system by developing innovative smart devices and using IoT technologies for ecosystem monitoring in isolated environments. The developed smart devices include four categories: large-scale and nonintrusive instruments to measure evapotranspiration and soil moisture, in situ observing systems for CO2 and d13 C associated with soil respiration, portable and distributed devices for monitoring vegetation variables, and Bi-CMOS cameras and pressure trigger sensors for terrestrial vertebrate monitoring. These new devices outperform conventional devices and are connected to each other via wireless communication networks. The breakthroughs in the ecosystem monitoring IoT include new data loggers and longdistance wireless sensor network technology that supports the rapid transmission of data from devices to wireless networks. The applicability of this ecosystem monitoring IoT is verified in three fragile ecosystems, including a karst rocky desertification area, the National Park for Amur Tigers, and the oasis-desert ecotone in China. By integrating these devices and technologies with an ecosystem monitoring information system, a seamless data acquisition, transmission, processing, and application IoT is created. The establishment of this ecosystem monitoring IoT will serve as a new paradigm for ecosystem monitoring and therefore provide a platform for ecosystem management and decision making in the era of big data. | Xin Li Ning Zhao Rui Jin Shaomin Liu Xiaomin Sun Xuefa Wen Dongxiu Wu Yan Zhou Jianwen Guo Shiping Chen Ziwei Xu Mingguo Ma Tianming Wang Yonghua Qu Xinwei Wang Fangming Wu Yuke Zhou | 2019 | Science Bulletin2019，64，17： | 10 |
| 2 | An H3K27me3 demethylase-HSFA2 regulatory loop orchestrates transgenerational thermomemory in Arabidopsis显示文摘Global warming has profound effects on plant growth and fitness.Plants have evolved sophisticated epigenetic machinery to respond quickly to heat,and exhibit transgenerational memory of the heat-induced release of post-transtriptional gene silencing(PTGS).However,how thermomemory is transmitted to progeny and the physiological relevance are elusive.Here we show that heat-induced HEAT SHOCK TRANSCRIPTION FACTOR A2(HSFA2)directly activates the H3K27me3 demethylase RELATIVE OF EARLY FLOWERING 6(REF6),which in turn derepresses HSFA2.REF6 and HSFA2 establish a heritable feedback loop,and activate an E3 ubiquitin ligase,SUPPRESSOR OF GENE SILENCING 3(SGS3)-INTERACTING PROTEIN 1(SGIP1).SGIP1-mediated SGS3 degradation leads to inhibited biosynthesis of trans-acting siRNA(tasiRNA).The REF&HSFA2 loop and reduced tasiRNA converge to release HEAT-INDUCED TAS1 TARGET 5(HTT5),which drives early flowering but attenuates immunity.Thus,heat induces transmitted phe no types via a coordi nated epigenetic network involvi ng histone demethylases,tran scriptio n factors,and tasiRNAs,ensuring reproductive success and transgenerational stress adaptation. | Junzhong Liu Lili Feng Xueting Gu Xian Deng Qi Qiu Qun Li Yingying Zhang Muyang Wang Yiwen Deng Ertao Wang Yuke He Isabel Baurle Jianming Li Xiaofeng Cao Zuhua He | 2019 | Cell Research2019，29，5： | 10 |
| 3 | A modified high-efficiency thermal asymmetric interlaced PCR method for amplifying long unknown flanking sequences显示文摘Rapid and efficient isolation of unknown flanking DNA sequences adjacent to known regions is important for molecular biology research.For this purpose,several PCR-based methods have been reported,including inverse PCR(Uchiyama and Watanabe,2006),ligation-mediated PCR(Yan et al.,2003;Ballester et al.,2005;Wang et al.,2007;Trinh et al.,2012)and randomly primed PCR(Liu and Whittier,1995;Liu et al.,1995;Antal et al. | Jiantao Tan Qi Gong Suize Yu Yuke Hou Dongchang Zeng Qinlong Zhu Yao-Guang Liu | 2019 | Journal of Genetics and Genomics2019，46，7： | 7 |
| 4 | WSe2 2D p-type semiconductor-based electronic devices for information technology:Design,preparation,and applications显示文摘The pioneering exfoliation of monolayer tungsten diselenide has greatly inspired researchers toward semiconducting applications.WSe2 belongs to a family of transition-metal dichalcogenides.Similar to graphene,WSe2 and analogous dichalcogenides have layered structures with weak van der Waals interactions between two adjacent layers.First,the readers are presented with the fundamentals of WSe2,such as types,morphologies,and properties.Here,we report the characterization principles and practices such as microscopy,spectroscopy,and diffraction.Second,the methods for obtaining high-quality WSe2,such as exfoliation,hydrothermal and chemical vapor deposition,are briefly listed.With advantages of light weight,flexibility,and high quantum efficiency,2D materials may have a niche in optoelectronics as building blocks in p-n junctions.Therefore,we introduce a state-of-the-art demonstration of heterostructure devices employing the p-type WSe2 semiconductor.The device architectures include field-effect transistors,photodetectors,gas sensors,and photovoltaic solar cells.Due to its unique electronic,optical,and energy band properties,WSe2 has been increasingly investigated due to the conductivity of the p-type charge carrier upon palladium contact.Eventually,the dynamic research on WSe2 and van der Waals heterostructures is summarized to arouse the passion of the 2D research community. | Qilin Cheng Jinbo Pang Dehui Sun Jingang Wang Shu Zhang Fan Liu Yuke Chen Ruiqi Yang Na Liang Xiheng Lu Yanchen Ji Jian Wang Congcong Zhang Yuanhua Sang Hong Liu Weijia Zhou | 2020 | InfoMat2020，2，4： | 5 |
| 5 | Effects of Neo-Tethyan evolution on the petroleum system of Persian Gulf Superbasin显示文摘Considering the Neo-Tethyan tectonic process and the resulting environmental changes,a geodynamic model of“one-way train loading”is proposed to analyze the formation and evolution mechanism of the Persian Gulf Superbasin with the most abundant hydrocarbons in the world.The Persian Gulf Superbasin has long been in a passive continental margin setting since the Late Paleozoic in the process of unidirectional subduction,forming a superior regional space of hydrocarbon accu-mulation.During the Jurassic-Cretaceous,the Persian Gulf Superbasin drifted slowly at low latitudes,and developed multiple superimposed source-reservoir-caprock assemblages as a combined result of several global geological events such as the Hadley Cell,the Equatorial Upwelling Current,and the Jurassic True Polar Wander.The collision during the evolution of the foreland basin since the Cenozoic led to weak destruction,which was conducive to the preservation of oil and gas.Accordingly,it is be-lieved that the slow drifting and long retention in favorable climate zone of the continent are the critical factors for hydrocarbon enrichment.Moreover,the prospects of hydrocarbon potential in other continents in the Neo-Tethyan were proposed. | ZHU Rixiang ZHANG Shuichang WAN Bo ZHANG Wang LI Yong WANG Huajian LUO Beiwei LIU Yuke HE Zhiliang JIN Zhijun | 2023 | Petroleum Exploration and Development2023，50，1： | 4 |
| 6 | miR-4458 inhibits the epithelial-mesenchymal transition of hepatocellular carcinoma cells by suppressing the TGF-β signaling pathway via targeting TGFBR1显示文摘Hepatocellular carcinoma(HCC)is one of the most lethal cancers in the world.MicroRNAs play a pivotal role in the progression of various cancers.To date,very lttle attention has beerl paid to miR-4458.Therefore,the aim of our study was to explore the function and underlying molecular mech-anism of miR-4458 in HCC.We found that the expression of miR-4458 was reduced in HCC tissues and cell lines.Forced overexpression of miR-4458 inhibited the migration,invasion,and epithelial-mesenchymal transition(EMT)of HCC cells,while downregulation of miR-4458 promoted the aggressive phenotype.Furthermore,transforming growth factor beta receptor 1(TGFBR1),the modulator of the TGF-βsignaling pathway,was verified to be a novel target gene of miR-4458 by dual-luciferase reporter gene assay.Upregulated miR-4458 dramatically abolished TGFBR1 and p-Smad2/3 expression,thus blocking the TGF-βsig naling pathway.Moreover,restoration of TGFBR1 partially rescued the miR-4458-mediated suppressive effect on the migration,invasion,and EMT and reactivated the TGFβsignaling pathway in HCC cells.In summary,our findings first demonstrated a mechanism of miR 4458 in HCC cell migration,invasion,and EMT by regulating the TGFβsignaling pathway via directly targeting TGFBR1. | Yuke Zhang Kun Shi Hang Liu Wei Chen Yunhai Luo Xufu Wei Zhongjun Wu | 2020 | Acta Biochimica et Biophysica Sinica2020，52，5： | 4 |
| 7 | The N^6-adenine methylation in yeast genome profiled by single-molecule technology显示文摘The most common and abundant DNA modification is 5-methylcytosine(5mC),which has been well-established as an epigenetic mark regulating gene expression in eukaryotes(Jones,2012).Another DNA modification N^6-methyldeoxyadenosine(6mA),previously reported as a widespread DNA methylation in prokaryotes, | Zhe Liang Guoliang Yu Jingrong Liu Yuke Geng Jinghui Mao Depeng Wang Jiapeng Zhou Xiaofeng Gu | 2018 | Journal of Genetics and Genomics2018，45，4： | 3 |
| 8 | METTL5 stabilizes c-Myc by facilitating USP5 translation to reprogram glucose metabolism and promote hepatocellular carcinoma progression显示文摘Background:Hepatocellular carcinoma(HCC)is one of the most prevalent cancers in the world,with a high likelihood of metastasis and a dismal prognosis.The reprogramming of glucosemetabolism is critical in the development ofHCC.TheWarburg effect has recently been confirmed to occur in a variety of cancers,including HCC.However,little is known about the molecular biological mechanisms underlying the Warburg effect in HCC cells.In this study,we sought to better understand how methyltransferase 5,N6-adenosine(METTL5)controls the development of HCC and theWarburg effect.Methods:In the current study,quantitative real-time polymerase chain reaction and Western blotting were used to detect the expression of METTL5 in HCC tissues and cell lines.Several different cell models and animal models were established to determine the role of METTL5 in glucose metabolism reprogramming and the underlying molecularmechanism of HCC.Glutathione-S-transferase pulldown,coimmunoprecipitation,RNA sequencing,non-targeted metabolomics,polysome profiling,and luciferase reporter assays were performed to investigate the molecular mechanisms of METTL5 in HCC cells.Results:We discovered that METTL5 drove glucose metabolic reprogramming to promote the proliferation and metastasis of HCC.Mechanistically,upregulation of METTL5 promoted c-Myc stability and thus activated its downstream glycolytic genes lactate dehydrogenase A(LDHA),enolase 1(ENO1),triosephosphate isomerase 1(TPI1),solute carrier family 2 member 1(SLC2A1),and pyruvate kinase M2(PKM2).The c-Box and ubiquitin binding domain(UBA)regions of ubiquitin specific peptidase 5(USP5)binded to c-Myc protein and inhibited K48-linked polyubiquitination of c-Myc.Further study revealed that METTL5 controled the USP5 translation process,which in turn regulated the ubiquitination of c-Myc.Furthermore,we identified cAMP responsive element binding protein 1(CREB1)/P300 as a critical transcriptional regulator ofMETTL5 that promoted the transcription of METTL5 in HCC.In patient-derived tumor xenograft(PDX)models,adenovirus-mediated knockout of METTL5 had a good antitumor effect and prolonged the survival of PDX-bearing mice.Conclusions:These findings point to a novel mechanism by which CREB1/P300-METTL5-USP5-c-Myc controls abnormal glucose metabolism and promotes tumor growth,suggesting that METTL5 is a potential therapeutic target and prognostic biomarker for HCC. | Peng Xia Hao Zhang Haofeng Lu Kequan Xu Xiang Jiang Yuke Jiang Xiangdong Gongye Zhang Chen Jie Liu Xi Chen Weijie Ma Zhonglin Zhang Yufeng Yuan | 2023 | Cancer Communications2023，43，3： | 3 |
| 9 | Fine-scale activity patterns of large-and medium-sized mammals in a deciduous broadleaf forest in the Qinling Mountains,China显示文摘The composition of animal species and interactions among them are widely known to shape ecological communities and fine-scale(e.g.,<1 km)monitoring of animal communities is essential for understanding the relationships among animals and plants.Although the co-existence of large-and medium-sized species has been studied across different scales,research on fine-scale interactions of herbivores in deciduous broadleaf forests is limited.Camera trapping of large-and medium-sized mammals was carried out over a 1 year period within a 25 ha deciduous broadleaf forest dynamics plot in the Qinling Mountains,China.Fourteen species of large-and medium-sized mammals,including six carnivores,six ungulates,one primate and one rodent species were found.Kernel density estimations were used to analyse the diel or 24 h activity patterns of all species with more than 40 independent detections and general linear models were developed to explore the spatial relationships among the species.The combination of overlapping diel activity patterns and spatial associations showed obvious niche separation among six species:giant panda(Ailuropoda melanoleuca David),takin(Budorcas taxicolor Hodgson),Reeves’s muntjac(Muntiacus reevesi Ogilby),tufted deer(Elaphodus cephalophus Milne-Edwards),Chinese serow(Capricornis milneedwardsii David)and wild boar(Sus scrofa Linnaeus).Long-term fine-scale monitoring is useful for providing information about the co-existence of species and their interactions.The results demonstrate the importance for fine-scale monitoring of animals and plants for improving understanding of species interactions and community dynamics. | Yuke Zhang Xiangbo He Xuehua Liu Melissa Songer Haishan Dang Quanfa Zhang | 2021 | Journal of Forestry Research2021，32，6： | 1 |
| 10 | A Two-Phase Paradigm for Joint Entity-Relation Extraction显示文摘An exhaustive study has been conducted to investigate span-based models for the joint entity and relation extraction task.However,these models sample a large number of negative entities and negative relations during the model training,which are essential but result in grossly imbalanced data distributions and in turn cause suboptimal model performance.In order to address the above issues,we propose a two-phase paradigm for the span-based joint entity and relation extraction,which involves classifying the entities and relations in the first phase,and predicting the types of these entities and relations in the second phase.The two-phase paradigm enables our model to significantly reduce the data distribution gap,including the gap between negative entities and other entities,aswell as the gap between negative relations and other relations.In addition,we make the first attempt at combining entity type and entity distance as global features,which has proven effective,especially for the relation extraction.Experimental results on several datasets demonstrate that the span-based joint extraction model augmented with the two-phase paradigm and the global features consistently outperforms previous state-ofthe-art span-based models for the joint extraction task,establishing a new standard benchmark.Qualitative and quantitative analyses further validate the effectiveness the proposed paradigm and the global features. | Bin Ji Hao Xu Jie Yu Shasha Li JunMa Yuke Ji Huijun Liu | 2023 | Computers, Materials & Continua2023，，1： | 1 |
| 11 | BcpLH organizes a specific subset of microRNAs to form a leafy head in Chinese cabbage(Brassica rapa ssp.pekinensis)显示文摘HYL1(HYPONASTIC LEAVES 1)in Arabidopsis thaliana encodes a double-stranded RNA-binding protein needed for proper miRNA maturation,and its null mutant hyl1 shows a typical leaf-incurvature phenotype.In Chinese cabbage,BcpLH(Brassica rapa ssp.pekinensis LEAFY HEADS),a close homolog of HYL1,is differentially expressed in juvenile leaves,which are flat,and in adult leaves,which display extreme incurvature.BcpLH lacks protein–protein interaction domains and is much shorter than HYL1.To test whether BcpLH is associated with defects in microRNA(miRNA)biogenesis and leaf flatness,we enhanced and repressed the activity of BcpLH by transgenics and investigated BcpLH-dependent miRNAs and plant morphology.BcpLH promoted miRNA biogenesis by the proper processing of primary miRNAs.BcpLH downregulation via antisense decreased a specific subset of miRNAs and increased the activities of their target genes,causing upward curvature of rosette leaves and early leaf incurvature,concurrent with the enlargement,earliness,and round-to-oval shape transition of leafy heads.Moreover,BcpLH-dependent miRNAs in Chinese cabbage are not the same as HYL1-dependent miRNAs in Arabidopsis.We suggest that BcpLH controls a specific subset of miRNAs in Chinese cabbage and coordinates the direction,extent,and timing of leaf curvature during head formation in Brassica rapa. | Wenqing Ren Feijie Wu Jinjuan Bai Xiaorong Li Xi Yang Wanxin Xue Heng Liu Yuke He | 2020 | Horticulture Research2020，7，1： | 1 |
| 12 | Seryl tRNA synthetase cooperates with POT1 to regulate telomere length and cellular senescence显示文摘Deregulated telomere length is a causative factor in many physiological and pathological processes,including aging and cancer.Many studies focusing on telomeres have revealed important roles for cooperation between the Shelterin protein complex and telomerase in maintaining telomere length.However,it remains largely unknown whether and how aging-related stresses,such as deregulated protein homeostasis,impact telomere length.Here,we explored the possible roles of aminoacyl tRNA synthetases(AARSs),key enzymes catalyzing the first reactions in protein synthesis,in regulating telomere length and aging.We selected seryl tRNA synthetase(SerRS)since our previous studies discovered expanded functions of SerRS in the nucleus in addition to its canonical cytoplasmic role in protein synthesis.In this study,we revealed that overexpression of SerRS promoted cellular senescence and inhibited the growth of cervical tumor xenografts in mice by triggering the senescence of tumor cells.In the nucleus,SerRS directly bound to telomeric DNA repeats and tethered more POT1 proteins to telomeres through a direct interaction between the UNE-S domain of SerRS and the OB1 domain of POT1.We further demonstrated that SerRS-induced enrichment of POT1 prevented the recruitment of telomerase to telomeres,resulting in progressive telomere shortening.Our data suggested a possible molecular link between protein synthesis and telomere length control,the deregulation of which may be associated with aging and cancer. | Yingxi Li Xiyang Li Mei Cao Yuke Jiang Jie Yan Ze Liu Rongcun Yang Xu Chen Peiqing Sun Rong Xiang Longlong Wang Yi Shi | 2019 | Signal Transduction and Targeted Therapy2019，4，1： | 1 |
| 13 | Water-dominated vegetation activity across biomes in mid-latitudinal eastern China显示文摘 | Wu Xiuchen Liu Hongyan Ren Ji He Siyuan Zhang Yuke | 2009 | Geophysical Research Letters2009，，4： | 1 |
| 14 | Effects of Mor- phine, Fentanyl and Tramadol on Human Immune Re- sponse显示文摘 | LIU Zhihen GAO Feng TIAN Yuke | 2006 | Journal of Huazhong University of Science and Technology: Medical Edition2006，26，4： | 1 |
| 15 | MoC nanoclusters anchored Ni@N‐doped carbon nanotubes coated on carbon fiber as three‐dimensional and multifunctional electrodes for flexible supercapacitor and self‐heating device显示文摘With the rapid development of different kinds of wearable electronic devices,flexible and high‐capacity power sources have attracted increasing attention.In this study,a facile strategy to fabricate Ni nanoparticles embedded in N‐doped carbon nanotubes(CNTs)(Ni@NCNTs)homogeneously coated on the surface of carbon fiber with a multistructural component of molybdenum carbide(MoC/Ni@NCNTs/CC)was synthesized.There are two forms of MoC in MoC/Ni@NCNTs/CC,including the MoC nanoclusters in a size of 2 to 4 nm anchored on Ni@N‐doped CNTs and the MoC nanoparticles as an interface between MoC/Ni@NCNTs and carbon cloth(CC).Multifunctional MoC/Ni@NCNTs/CC served as both positive and negative electrode and a heater in flexible supercapacitors and in wearable devices,which exhibited excellent electrochemical and heating performance.Besides,an all‐solid‐state supercapacitor consists of two pieces of MoC/Ni@NCNTs/CC that exhibited extraordinary energy storage performance with high‐energy density(78.7μWh/cm2 at the power density of 2.4 mW/cm2)and excellent cycling stability(≈91%capacity retention after 8000 cycles).Furthermore,all‐solid‐state flexible supercapacitors were incorporated with an MoC/Ni@NCNTs/CC electrode into self‐heating flexible devices for keeping the human body warm.Thus,MoC/Ni@NCNTs/CC is a promising electrode material for flexible and wearable storage systems and heating electronic application. | Fan Liu Jietong He Xiaoyu Liu Yuke Chen Zhen Liu Duo Chen Hong Liu Weijia Zhou | 2021 | Carbon Energy2021，3，1： | 1 |
| 16 | A novel method for solving shortest tool length based on compressing 3D check surfaces relative to tool postures显示文摘Solving the shortest tool length quickly under a known tool trajectory in multi-axis machining of complex channel parts is an urgent problem in industrial production. To solve this problem, a novel and efficient method is proposed which is featured by extracting only a few necessary curves from the check surface instead of sampling the entire surface. By rotating and compressing the 3 D check surface relative to all tool postures, the boundaries of the area occupied by the 2 D compressed surfaces are the essential elements for determining the shortest tool length. A tracking-based numerical algorithm is introduced to efficiently solve the silhouette curves which are formed in compressing. To define the multi-taper shaped tool holding system(THS) which is commonly used in production, a characterization model for THS profile is established. A model for solving the shortest tool length is finally constructed based on the critical interference relationship between the THS profile and all compressed boundary curves. For acceleration, the boundary splines are segmented according to their knot vectors. Then a new concept called the axis-aligned tool length box(AATB) is introduced,which can provide a conservative range of tool length for a spline segment. By scanning the AATBs of all spline segments, the very few effective spline segments that may ultimately determine the shortest tool length are filtered out. This acceleration method makes the solution for the shortest tool length more focused and efficient. The results of experimental examples are also reported to validate the efficiency and accuracy of the proposed algorithm. | Xiangyu LI Junxue REN Ximing LV Yuke ZHOU Congle LIU | 2021 | Chinese Journal of Aeronautics2021，34，2： | 1 |
| 17 | Gulong shale oil enrichment mechanism and orderly distribution of conventional–unconventional oils in the Cretaceous Qingshankou Formation,Songliao Basin,NE China显示文摘Through the study of organic matter enrichment,hydrocarbon generation and accumulation process of black shale of the Cretaceous Qingshankou Formation in the Songliao Basin,the enrichment mechanism of Gulong shale oil and the distribution of conventional–unconventional oil are revealed.The Songliao Basin is a huge interior lake basin formed in the Early Cretaceous under the control of the subduction and retreat of the western Pacific plate and the massive horizontal displacement of the Tanlu Fault Zone in Northeast China.During the deposition of the Qingshankou Formation,strong terrestrial hydrological cycle led to the lake level rise of the ancient Songliao Basin and the input of a large amount of nutrients,resulting in planktonic bacteria and algae flourish.Intermittent seawater intrusion events promoted the formation of salinization stratification and anoxic environment in the lake,which were beneficial to the enrichment of organic matters.Biomarkers analysis confirms that the biogenic organic matter of planktonic bacteria and algae modified by microorganisms plays an important role in the formation of high-quality source rocks with high oil generation capability.There are four favorable conditions for the enrichment of light shale oil in the Qingshankou Formation of the Gulong Sag,Songliao Basin:the moderate organic matter abundance and high oil potential provide sufficient material basis for oil enrichment;high degree of thermal evolution makes shale oil have high GOR and good mobility;low hydrocarbon expulsion efficiency leads to a high content of retained hydrocarbons in the source rock;and the confinement effect of intra-layer cement in the high maturity stage induces the efficient accumulation of light shale oil.The restoration of hydrocarbon accumulation process suggests that liquid hydrocarbons generated in the early(low–medium maturity)stage of the Qingshankou Formation source rocks accumulated in placanticline and slope after long-distance secondary migration,forming high-quality conventional and tight oil reservoirs.Light oil generated in the late(medium–high maturity)stage accumulated in situ,forming about 15 billion tons of Gulong shale oil resources,which finally enabled the orderly distribution of conventional–unconventional oils that are contiguous horizontally and superposed vertically within the basin,showing a complete pattern of“whole petroleum system”with conventional oil,tight oil and shale oil in sequence. | ZHANG Shuichang ZHANG Bin WANG Xiaomei FENG Zihui HE Kun WANG Huajian FU Xiuli LIU Yuke YANG Chunlong | 2023 | Petroleum Exploration and Development2023，50，5： | 0 |
| 18 | Palladium-Catalyzed One-Step Synthesis of Stereodefined Difunctionalized Glycals显示文摘Efficiently modifying glycals by directly introducing functional groups into their double bonds is a longstanding challenge.Here,the strategy of introducing two different functional groups into 1-iodoglycals to obtain modified glycals in one step through palladium catalysis was reported for the first time,and the modified glycals contained stereodefined tetrasubstituted olefins.Using this method,various difunctionalized glycals that were difficult to form by other routes were synthesized in moderate to good yields.Control experiments and density functional theory calculations show that the palladium catalyst played dual roles in this transformation,namely,inducing nucleophilic substitution and catalyzing Suzuki coupling.The reaction intermediate was isolated and confirmed by X-ray crystallographic analysis.Furthermore,the gram-scale synthesis and facile deprotection of the target compound enhances the practicality of this strategy. | Xiao-Ping Gong Yuke Li Hong-Chao Liu Zhe Zhang Zhi-Jie Niu Ya-Nan Ding Yang An Xi Chen Yan-Chong Huang Rui-Qiang Jiao Xue-Yuan Liu Yong-Min Liang | 2023 | CCS Chemistry2023，5，3： | 0 |
| 19 | TaTIP41 and TaTAP46 positively regulate drought tolerance in wheat by inhibiting PP2A activity显示文摘Drought is a major environmental stress limiting global wheat(Triticum aestivum)production.Exploring drought tolerance genes is important for improving drought adaptation in this crop.Here,we cloned and characterized TaTIP41,a novel drought tolerance gene in wheat.TaTIP41 is a putative conserved component of target of rapamycin(TOR)signaling,and the Ta TIP41 homoeologs were expressed in response to drought stress and abscisic acid(ABA).The overexpression of Ta TIP41 enhanced drought tolerance and the ABA response,including ABA-induced stomatal closure,while its downregulation using RNA interference(RNAi)had the opposite effect.Furthermore,Ta TIP41 physically interacted with TaTAP46,another conserved component of TOR signaling.Like TaTIP41,TaTAP46 positively regulated drought tolerance.Furthermore,TaTIP41 and TaTAP46 interacted with type-2A protein phosphatase(PP2A)catalytic subunits,such as TaPP2A-2,and inhibited their enzymatic activities.Silencing TaPP2A-2 improved drought tolerance in wheat.Together,our findings provide new insights into the roles of TaTIP41 and TaTAP46 in the drought tolerance and ABA response in wheat,and their potential application in improving wheat environmental adaptability. | Jianhui Ma Yuke Geng Hong Liu Mengqi Zhang Shujuan Liu Chenyang Hao Jian Hou Youfu Zhang Daijing Zhang Weijun Zhang Xueyong Zhang Tian Li | 2023 | Journal of Integrative Plant Biology2023，65，9： | 0 |
| 20 | A conserved unusual posttranscriptional processing mediated by short,direct repeated (SDR) sequences in plants显示文摘In several stress responsive gene loci of monocot cereal crops,we have previously identified an unusual posttranscriptional processing mediated by paired presence of short direct repeated (SDR) sequences at 5' and 3' splicing junctions that are distinct from conventional (U2/U12-type) splicing boundaries.By using the known SDR-containing sequences as probes,24 plant candidate genes involved in diverse functional pathways from both monocots and dicots that potentially possess SDR-mediated posttranscriptional processing were predicted in the GenBank database.The SDRs-mediated posttranscriptional processing events including cis-and trans-actions were experimentally detected in majority of the predicted candidates.Extensive sequence analysis demonstrates several types of SDR-associated splicing peculiarities including partial exon deletion,exon fragment repetition,exon fragment scrambling and trans-splicing that result in either loss of partial exon or unusual exonic sequence rearrangements within or between RNA molecules.In addition,we show that the paired presence of SDR is necessary but not sufficient in SDR-mediated splicing in transient expression and stable transformation systems.We also show prokaryote is incapable of SDR-mediated premRNA splicing. | Xiangli Niu Di Luo Shaopei Gao Guangjun Ren Lijuan Chang Yuke Zhou Xiaoli Luo Yuxiang Li Pei Hou Wei Tang Bao-Rong Lu Yongsheng Liu | 2010 | Journal of Genetics and Genomics2010，37，1： | 0 |
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