|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Lentinan administration alleviates diarrhea of rotavirus-infected weaned pigs via regulating intestinal immunity显示文摘Background:Lentinan(LNT)may regulate many important physiological functions of human and animals.This study aimed to verify whether LNT administration could relieve diarrhea via improving gut immunity in rotavirus(RV)-challenged weaned pigs.Methods:Twenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d(n=14).RV infection was executed on d 15.After extracting polysaccharides from LNT product,its major monosaccharides were analyzed.Then,LNT polysaccharide was used to administrate RV-infected IPEC-J2 cells.Results:Dietary LNT supplementation supported normal function of piglets even when infected with RV,as reflected by reduced growth performance loss and diarrhea prevalence,and maintained gut immunity(P<0.05).The polysaccharide was isolated from LNT product,which molecular weight was 5303 Da,and major monosaccharides included glucose,arabinose and galactose.In RV-infected IPEC-J2 cells,this polysaccharide significantly increased cell viability(P<0.05),and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides(P<0.05).Conclusion:Those results suggest that LNT administration increases the piglets’resistance to RV-induced stress,likely by supporting intestinal immunity. | Xiangqi Fan Haiyan Hu Daiwen Chen Bing Yu Jun He Jie Yu Junqiu Luo Erik Eckhardt Yuheng Luo Jianping Wang Hui Yan Xiangbing Mao | 2021 | Journal of Animal Science and Biotechnology2021，12，3： | 8 |
| 2 | Is graphite lithiophobic or lithiophilic?显示文摘Graphite and lithium metal are two classic anode materials and their composite has shown promising performance for rechargeable batteries.However,it is generally accepted that Li metal wets graphite poorly,causing its spreading and infiltration difficult.Here we show that graphite can either appear superlithiophilic or lithiophobic,depending on the local redox potential.By comparing the wetting performance of highly ordered pyrolytic graphite,porous carbon paper(PCP),lithiated PCP and graphite powder,we demonstrate that the surface contaminants that pin the contact-line motion and cause contact-angle hysteresis have their own electrochemical-stability windows.The surface contaminants can be either removed or reinforced in a time-dependent manner,depending on whether the reducing agents(C6→Li C6)or the oxidizing agents(air,moisture)dominate in the ambient environment,leading to bifurcating dynamics of either superfast or superslow wetting.Our findings enable new fabrication technology for Li–graphite composite with a controllable Li-metal/graphite ratio and present great promise for the mass production of Li-based anodes for use in high-energy-density batteries. | Jian Duan Yuheng Zheng Wei Luo Wangyan Wu Tengrui Wang Yong Xie Sa Li Ju Li Yunhui Huang | 2020 | National Science Review2020，7，7： | 8 |
| 3 | Dietary lycopene supplementation improves meat quality,antioxidant capacity and skeletal muscle fiber type transformation in finishing pigs显示文摘This study aimed to investigate effects of dietary lycopene supplementation on meat quality,antioxidant ability and muscle fiber type transformation in finishing pigs.In a 70-day experiment,18 DurocLandraceYorkshire barrows were randomly allocated to 3 dietary treatments including a basal diet supplemented with 0,100 and 200 mg/kg lycopene,respectively.Each dietary treatment had 6 replicates with one pig each.Results showed that dietary 200 mg/kg lycopene supplementation increased muscle redness a^(*)value,intramuscular fat and crude protein contents,and decreased muscle lightness L^(*)and yellowness b^(*)values(P<0.05),suggesting that addition of 200 mg/kg lycopene to the diet of finishing pigs improved color,nutritional value and juiciness of pork after slaughter.Results also showed that dietary lycopene supplementation enhanced antioxidant capacity of finishing pigs(P<0.05).Moreover,dietary supplementation of 200 mg/kg lycopene significantly increased slow myosin heavy chain(MyHC)protein level and slow-twitch fiber percentage,and decreased fast MyHC protein level and fast-twitch fiber percentage(P<0.05),suggesting that the addition of 200 mg/kg lycopene to the diet of finishing pigs promoted muscle fiber type conversion from fast-twitch to slow-twitch.Together,we provide the first evidence that dietary 200 mg/kg lycopene supplementation improves meat quality,enhances antioxidant capacity and promotes muscle fiber type transformation from fast-twitch to slow-twitch in finishing pigs. | Wanxue Wen Xiaoling Chen Zhiqing Huang Daiwen Chen Bing Yu Jun He Yuheng Luo Hui Yan Hong Chen Ping Zheng Jie Yu | 2022 | Animal Nutrition2022，，1： | 8 |
| 4 | Tannic acid extracted from gallnut prevents post-weaning diarrhea and improves intestinal health of weaned piglets显示文摘This experiment was conducted to evaluate the effects of different levels of tannic acid(TA)on growth performance,diarrhea rate,nutrient digestibility and intestinal health in weaned piglets.A total of 180 weaned piglets(Duroc×Landrace×Yorkshire,24 d of age,initial average BW=7.77±0.17 kg)were allotted to 5 groups(6 pigs/pen and 6 replicates/group)in a randomized complete block design according to their gender and body weight.Piglets were fed a basal diet,or the basal diet supplemented with 0.05%,0.1%,0.2%or 0.4%TA for 28 d.The supplementary levels of TA in the diets were obtained by adding tannalbin containing 51%TA and 40.17%protein.The results showed that,compared with the CON group,dietary TA did not affect ADFI,ADG or F:G,and linearly reduced(P<0.01)the diarrhea rate and diarrhea index of piglets.There were no significant effects on apparent total tract digestibility(ATTD)in the 0.05%,0.1%and 0.2%TA groups,while negative effects(P<0.05)on apparent digestibility of crude protein and gross energy were observed in the 0.4%TA group.In addition,the nutrient digestibility of dry matter,crude protein and gross energy linearly decreased(P<0.01)with the increase of TA dosage.Supplementation of TA increased(P<0.05)the villus height of the duodenum and jejunum,as well as increased(P<0.05)catalase(CAT)activity in serum.Dietary TA improved(P<0.05)the Bacillus counts in cecal digesta.Further,TA significantly improved(P<0.05)Bacillus counts and reduced(P<0.05)the Escherichia coli counts in colonic digesta.The concentration of acetic acid,pro pionic acid,butyric acid and isovaleric acid in cecal digesta were significantly increased(P<0.05).The mRNA expression level of zonula occludens-1(Z0-1),zonula occludens-2(ZI-2),and claudin-2(CLDN-2)in the jejunum were greater(P<0.05)in TA supplemented groups.The study showed that,compared to the control,TA prevented post-weaning diarrhea and improved intestinal health of weaned piglets,and the appropriate level of TA supplementation would be from 0.1%to 0.2%. | Yanyan Song Yong Luo Bing Yu Jun He Ping Zheng Xiangbing Mao Zhiqing Huang Junqiu Luo Yuheng Luo Hui Yan Quyuan Wang Huifen Wang Daiwen Chen Jie Yu | 2021 | Animal Nutrition2021，，4： | 5 |
| 5 | Differential expression, molecular cloning,and characterization of porcine beta defensin 114显示文摘Background:β-defensins have attracted considerable research interest because of their roles in protecting hosts from various pathogens.This study was conducted to investigate the expression profiles of the porcineβ-defensin114(PBD114)in different breeds and in response to infections.Moreover,the function of PBD114 protein was partially investigated.Methods:Six Tibetan pigs(TP)and six DLY(Duroc×Landrace×Yorkshire)pigs were slaughtered to explore the expression profiles of PBD114 in different breeds and tissues.For infection models,sixteen DLY pigs were divided into two groups and challenged either with sterile saline or E.coli K88.The recombinant protein PBD114(r PBD114)was obtained by using a heterologous expression system in E.coli.Results:PBD114 gene was highly expressed in tissues such as the intestine,liver,spleen,and thymus.Interestingly,the expression level of PBD114 gene was higher in the TP pigs than in the DLY pigs(P<0.05),and was significantly elevated upon E.coli K88 challenge(P<0.05).The nucleotide sequences of PBD114 from Tibetan and DLY pigs was identical,and both showed a 210-bp open reading frame encoding a 69-amino acid mature peptide.To explaore the function of PBD114 protein,PBD114 gene was successfully expressed in E.coli Origami B(DE3)and the molecular weight of the r PBD114 was estimated by SDS-PAGE to be 25 k Da.The r PBD114 was purified and mass spectrometry verified the protein as PBD114.Importantly,r PBD114 showed antimicrobial activities against E.coli DH5αand E.coli K88,and the minimal inhibitory concentrations(MICs)were 64 and 128μg/m L,respectively.Hemolytic and cytotoxicity assays showed that r PBD114 did not affect cell viability under physiological concentrations.Conclusions:PBD114 is an infection response gene that is differentially-expressed between different porcine breeds and tissues.The antimicrobial activity of PBD114 protein,against pathogens such as the E.coli K88,suggested that it may serve as a candidate for the substitution of conventionally used antibiotics. | Guoqi Su Kunhong Xie Daiwen Chen Bing Yu Zhiqing Huang Yuheng Luo Xiangbing Mao Ping Zheng Jie Yu Junqiu Luo Jun He | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 4 |
| 6 | Supplementing daidzein in diets improves the reproductive performance, endocrine hormones and antioxidant capacity of multiparous sows显示文摘Certain hormones play important roles in modulating mammalian reproductive behaviour.Daidzein is a well-known isoflavonic phytoestrogen that possesses oestrogenic activity.This study was conducted to probe the effects of daidzein supplementation in gestation diets on the reproductive performance in sows.A total of 120 multiparous sows(Landrace x Yorkshire)were randomly assigned to 2 groups(n=60)and fed either a base diet(control)or one containing 200 mg/kg daidzein during gestation.We discovered that daidzein supplementation significantly increased the total number of piglets born per litter and number of piglets born alive per litter(P<0.05),decreased the farrowing time(P<0.05)and increased the serum oestrogen and progesterone concentrations(P<0.05)at 35 d of gestation.Moreover,serum immunoglobulin G(IgG)concentration and superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activities were higher in the daidzein-treated group than in the control group at35 d of gestation(P<0.05).Daidzein increased the serum SOD activity and total anti-oxidative capacity(T-AOC)at 85 d of gestation(P<0.05).Interestingly,daidzein elevated the expression levels of the sodium-coupled neutral amino acid transporter 1(SLC38 A1)and insulin-like growth factor 1(IGF-1)genes in the placenta(P<0.05).These results suggest that daidzein ingestion could improve sow reproductive performance by changing serum hormones,elevating anti-oxidative capacity and upregulating critical functional genes in the placenta. | Yan Li Guoru He Daiwen Chen Bing Yu Jie Yu Ping Zheng Zhiqing Huang Yuheng Luo Junqiu Luo Xiangbing Mao Hui Yan Jun He | 2021 | Animal Nutrition2021，，4： | 4 |
| 7 | Rice metabolic regulatory network spanning the entire life cycle显示文摘As one of the most important crops in the world,rice(Oryza sativa)is a model plant for metabolome research.Although many studies have focused on the analysis of specific tissues,the changes in metab-olite abundance across the entire life cycle have not yet been determined.In this study,combining both tar-geted and nontargeted metabolite profiling methods,a total of 825 annotated metabolites were quantified in rice samples from different tissues covering the entire life cycle.The contents of metabolites in different tissues of rice were significantly different,with various metabolites accumulating in the plumule and radicle during seed germination.Combining these data with transcriptome data obtained from the same time period,we constructed the Rice Metabolic Regulation Network.The metabolites and co-expressed genes were further divided into 12 clusters according to their accumulation patterns,with members within each cluster displaying a uniform and clear pattern of abundance across development.Using this dataset,we established a comprehensive metabolic profile of the rice life cycle and used two independent strategies to identify novel transcription factors-namely the use of known regulatory genes as bait to screen for new networks underlying lignin metabolism and the unbiased identification of new glycerophospholipid metabolism regulators on the basis of tissue specificity.This study thus demonstrates how guilt-by-association analysis of metabolome and transcriptome data spanning the entire life cycle in cereal crops provides novel resources and tools to aid in understanding the mechanisms underlying important agro-nomic traits. | Chenkun Yang Shuangqian Shen Shen Zhou Yufei Li Yuyuan Mao Junjie Zhou Yuheng Shi Longxu An Qianqian Zhou Wenju Peng Yuanyuan Lyu Xuemei Liu Wei Chen Shouchuang Wang Lianghuan Qu Xianqing Liu Alisdair R.Fernie Jie Luo | 2022 | Molecular Plant2022，15，2： | 3 |
| 8 | Tannic acid prevents post-weaning diarrhea by improving intestinal barrier integrity and function in weaned piglets显示文摘Background:Tannic acid(TA)is potential to reduce diarrhea in weaning pigs,but knowledge about the influence of TA on intestinal barrier integrity and function is still scarce.This experiment was conducted to investigate the effects of dietary TA supplementation on growth performance,diarrhea rate,intestinal barrier integrity and function of weaned pigs.Methods:A total of 108 crossbred(Duroc×Landrace×Yorkshire)piglets,with an initial average body weight of 6.60±0.27 kg,were allotted to 3 groups(6 pigs/pen and 6 replicates/group)in a randomized complete block design according to their gender and body weight.Piglets were fed the basal diet with 0(control,CON),0.2%and 1.0%TA,respectively.The trial lasted for 28 d.Results:Compared with the CON group,dietary 0.2%and 1.0%TA supplementation didn’t affect ADFI,ADG and F:G(P>0.05),but reduced diarrhea rate,diarrhea index and diarrhea score of piglets(P<0.05),reduced diamine oxidase(DAO)activity and D-lactic acid concentration in serum(P<0.01).The higher occludin expression and localization were observed in the duodenum,jejunum and ileum after supplementation with 0.2%or 1.0%TA(P<0.05).Adding 0.2%TA to diet significantly decreased crypt depth,increased villus height/crypt depth ratio in the duodenum(P<0.05),and dietary 1.0%TA tended to decrease crypt depth(P<0.10)and significantly decreased villus height(P<0.05)of the ileum.Moreover,lower malondialdehyde content in the ileum was detected in the pigs fed 1.0%TA(P<0.05).In the duodenum,both 0.2%and 1.0%TA groups had higher occludin(OCLN)mRNA and 0.2%TA group had higher zonula occludens-2(ZO-2)level(P<0.05).Meanwhile,dietary 1.0%TA supplementation tended to up-regulate OCLN mRNA levels in the jejunum(P<0.10)and 0.2%TA supplementation tended to up-regulate zonula occludens-1(ZO-1)mRNA levels in the ileum(P<0.10).Conclusion:In conclusion,dietary supplementation of 0.2%or 1.0%TA could effectively alleviate postweaning diarrhea without altering growth performance in weaned piglets,which might be achieved by improving intestinal barrier integrity and function. | Jie Yu Yanyan Song Bing Yu Jun He Ping Zheng Xiangbing Mao Zhiqing Huang Yuheng Luo Junqiu Luo Hui Yan Quyuan Wang Huifen Wang Daiwen Chen | 2021 | Journal of Animal Science and Biotechnology2021，12，2： | 3 |
| 9 | Branched-chain amino acids regulate plant growth by affecting the homeostasis of mineral elements in rice显示文摘Dear Editor,Branched-chain amino acids(BCAAs)are essential amino acids that must be obtained from the diet for humans and animals.Branched-chain amino acid transaminases(BCATs)catalyze a key step in the BCAA synthesis pathway,however,little is known about their biological roles in planta.In this study,we functionally characterized two BCATs,OsBCAT4 and OsBCAT5,in rice and showed that they regulate the development of rice roots by affecting the levels of mineral elements. | Cheng Jin Yangyang Sun Yuheng Shi Yuanyuan Zhang Kai Chen Yuan Li Guige Liu Fang Yao Deng Cheng Jie Li Junjie Zhou Lianghuan Qu Xianqing Liu Jie Luo | 2019 | Science China(Life Sciences)2019，62，8： | 3 |
| 10 | Leucine modulates the IPEC-J2 cell proteome associated with cell proliferation, metabolism and phagocytosis显示文摘Leucine can affect intestinal protein expressions, and improve mucosal immune function. However, little study has been conducted to determine the change of protein component by leucine treatment in intestine epithelial cells. The present study was to cover the key proteins and cell pathways that could be regulated by leucine treatment in porcine intestinal epithelial cell line(IPEC-J2) cells with the approach of proteome analysis. A total number of 3,211 proteins were identified in our approach by searching the database of Uniprot sus scrofa. Among identified proteins, there were 101 proteins expressed differently between control group and leucine group. Compared with the control group, there were 50 up-regulated proteins and 51 down-regulated proteins in leucine group. In these proteins, leucine treatment decreased the expression of some proteins including pyruvate kinase, glyceraldehyde-3-phosphate dehydrogenase,E3 ubiquitin ligase, cathepsin D, caspase 3 and caspase 6, and increased the levels of some proteins, such as some eukaryotic translation initiation factors, ubiquitin carboxyl-terminal hydrolase, DNA-related RNA polymerase II, urokinase plasminogen activator, cyclin-dependent kinase inhibitor 2 b, Mut L homolog 1,5-methylcytosine binding domain 4, polymerase d, a-tubulin, syntaxin 18, Ras homolog D, actin related protein 2/3 complex and cofilin. Via the analysis of Gene Ontology and pathways, these proteins in IPECJ2 cells were related with some physiological functions, such as protein metabolism, glycolysis, cell proliferation, apoptosis and phagocytosis. Thus, these results suggest that leucine affects gut barrier function possibly via regulating cell proliferation and apoptosis, metabolism and phagocytosis. | Xiangbing Mao Man Ren Daiwen Chen Bing Yu Lianqiang Che Jun He Junqiu Luo Yuheng Luo Jianping Wang Hui Sun | 2018 | Animal Nutrition2018，，3： | 2 |
| 11 | Lower abundance of Bacteroides and metabolic dysfunction are highly associated with the post-weaning diarrhea in piglets显示文摘Growing evidences show a direct link between diarrhea and disorders of gut microbiota in pigs.However,whether there are microbial markers associated with post-weaning diarrhea remains unknown.In the current study,we compared the microbial community,functions and metabolites between healthy weaned piglets(group H,n=7)and piglets with post-weaning diarrhea(group D,n=7),in order to find out diarrhea associated microbial markers.Each of 7 fecal samples was collected from H and D piglets(weaned at 21 d and sampled at 26 d).The metagenomic and untargeted metabolomic analysis revealed that the microbial composition,function and metabolic profile in D pigs was considerably reshaped,including the reduced abundance and number of Bacteroides,which significantly correlated with the diarrhea status of host.The carbohydrate metabolism,biosynthesis and metabolism,lipid metabolism,amino acid metabolism,and the activity of glycan and carbohydrates digestion related enzymes showed extensively down-regulated in D pigs compared with H pigs.Diarrhea significantly changed the metabolic profiles of fecal microbiota,and most of the altered metabolites were negatively or positively correlated with the change in the abundance of Bacteroides.In conclusion,the lower abundance of Bacteroides and its associated metabolic dysfunction may be regarded as microbial markers of physiological post-weaning diarrhea in piglets. | Wen Ren Bing Yu Jie Yu Ping Zheng Zhiqing Huang Junqiu Luo Xiangbing Mao Jun He Hui Yan Jinlong Wu Daiwen Chen Yuheng Luo | 2022 | Science China(Life Sciences)2022，65，10： | 2 |
| 12 | Diversity of methanogens in the hindgut of captive white rhinoceroses,Ceratotheriumsimum显示文摘 | Luo Yuheng Li Hua Andre-Denis G Wright | | 0，，： | 1 |
| 13 | Effects of Compound Acidifier and Probiotics on Production Performance,Blood Biochemical Indexes,Antioxidant Indexes and Salmonella Infection of Laying Hens显示文摘This study evaluated the effects of compound acidifier and probiotics on production performance,blood biochemical indexes,antioxidant indexes and Salmonella infection of laying hens. A total of 600 50-week-old healthy laying hens with similar laying rate were randomly assigned to four groups,10 repeats each group,and each repeat contained 15 chickens. The laying hens in control group were fed a basal diet,and in the other three experimental groups were fed basal diets supplemented with 300 g/t probiotics,0. 1% compound acidifier in the drinking water every other day,and 300 g/t probiotics + 0. 1% compound acidifier in the drinking water every other day,respectively. After the feeding of 16 weeks,a total of 60 laying hens from control group were divided into four treatments as the same above for a 13-day long Salmonella challenge( 1. 3 × 10~8 CFU) trial. The results were shown as follows: no significant effects were observed on production performance,hemoglobin content,the superoxide dismutase activity and total antioxidant capacity of reproductive organ in compound acidifier and probiotics groups( P >0. 05). Dirty egg rate was significantly decreased in compound acidifier group,probiotics group and combination group of both( P <0. 05). Compared with the control group,white blood cell count in probiotics group was significantly decreased( P < 0. 05),and the immunoglobulin content in probiotics group tended to be increased( P = 0. 061). The malondialdehyde( MDA) content in oviduct isthmus of hens in compound acidifier group and combination group was significantly decreased compared with the control group( P < 0. 05). The Salmonella positive rate in oviduct of hens in compound acidifier group was significantly decreased at the 7^(th) and 14^(th) days( P < 0. 05),but that in probiotics group and combination group was not significantly different compared with control group( P > 0. 05). In conclusion,compound acidifier and probiotics can improve health status of laying hens through decreasing the dirty egg rate and increasing antioxidant capacity and immunity of them,thus reducing Salmonella infection of body and eggs. | Zhou Ling Ding Xuemei Luo Yuheng Wang Jianping Bai Shiping Meng Huaiwang Zhang Keying | 2017 | Animal Husbandry and Feed Science2017，9，3： | 1 |
| 14 | Cerium and carbon-sulfur codoped mesoporous TiO_(2)nanocomposites for boosting visible light photocatalytic activity显示文摘Ce and C-S codoped mesoporous TiO_(2)nanocomposites were synthesized via a sol-gel method integrated with an evaporation-induced self-assembly approach.The basic physicochemical characteristics of the synthetic samples were analyzed via a series of characterization techniques.The results reveal that C-S and Ce codoping on mesoporous TiO_(2)enhances the photocatalytic activity owing to the synergistic effect caused by narrowing the band gap,enhancing adsorption,trapping and transferring the excited e^(-)/h^(+)pairs and suppressing the recombination of e^(-)/h^(+)pairs.Furthermore,the obtained C,S-TiO_(2)/CeO_(2)materials exhibit large specific surface areas and numerous pores which not only effectively improve the adsorption-enrichment capability,but also supply multi-dimensional mass and electron transfer channels.The photodegradation efficiency of RhB by C,S-TiO_(2)/CeO_(2)within 40 min is nearly 100%,and its degradation efficiency is 6.63 times that of undoped TiO_(2).Recycling experiments show that mesoporous C,S-TiO_(2)/CeO_(2)shows excellent recoverability and stability.Furthermore,by trapping experiments,·O_(2)e^(-)/h^(+)and·OH are the predominant active species and a possible reaction mechanism is proposed. | Fei Zheng Faqin Dong Lin Zhou Jieyu Yu Xijie Luo Xingyu Zhang Zhenzhen Lv Luman Jiang Yuheng Chen Mengqing Liu | 2023 | Journal of Rare Earths2023，41，4： | 1 |
| 15 | Cell cycle synchronization by nutrient modulation显示文摘 | Tian Yuan Luo Chunxiong Lu Yuheng | 2012 | Integrative Biology2012，，3： | 1 |
| 16 | Effects of a multi-enzyme complex on growth performance,nutrient utilization and bone mineralization of meat duck显示文摘Background:Previous studies with broiler have shown dietary supplementation with multi-enzyme complex containing non-starch polysaccharides(NSP) degrading enzymes and phytase is efficient in releasing phosphorus(P),calcium(Ca),energy and amino acids from corn-soybean meal diets or corn-sorghum diets,hence compensating considerable levels of nutrients in formulation.Notwithstanding,such potentials have not been well defined in duck nutrition.Giving China being the largest duck producing country,we conducted this study to establish adequate specifications of major nutrients along with multi-enzyme complex to meat duck from day-old to slaughter,focusing on performance,utilization of nutrients and bone mineralization.Five dietary treatments were:Positive control(PC,T1):the nutrients concentration of diet for 1 to 14 d of age were apparent metabolizable energy(AME) 2,800 kcal/kg,crude protein(CP)19.39%,Ca 0.85%,available phosphorus(avP) 0.42%;for 15 to 35 d of age these parameters were AME2,900 kcal/kg,CP 16.47%,Ca 0.76%,avP 0.38%;Negative control 1(NC1,T2),the AME and digestible amino acids(DAA)were reduced by 70 kcal/kg and 2.0%,avP and Ca by 1.0 g/kg from PC diet;Negative control 2(NC2T4),the downspec from PC diet was AME 100 kcal/kg,DAA 2.5%,avP 1.5 g/kg and Ca 1.2 g/kg;The enzyme complex was added at the same dosage(200 ml7 1,000 kg) on NCI(T3) and NC2(T5) diets.Results:Comparing with the ducks fed on T1,T3 and T5 diets,the birds fed on NC2 diet showed the lowest(P < 0.05)body weight(d 14 and 35),feed intake(d 35),tibia ash,Ca and P contents(d 14 and 35),and the utilization of nutrients(P< 0.05).The supplementation with the enzyme complex to the NC diets restored growth rate,utilization of nutrients and bone mineralization to the level of the PC diet,and increased AME by 60 kcal/kg and117 kcal/kg,respectively for the NCI and NC2 diets.Conclusion:These results suggest that down-spec AME by 100 kcal/kg,DAA by 2.5%,avP by 1.5 g/kg and Ca by1.2 g/kg caused detrimental effects on duck performance compared with those fed on the PC diet,and these performance losses can be compensated by the addition of the multiple-enzyme complex. | Qiufeng Zeng Xueqin Huang Yuheng Luo Xuemei Ding Shiping Bai Jianping Wang Yue Xuan Zhuowei Su Yonggang Liu Keying Zhang | 2015 | Journal of Animal Science and Biotechnology2015，6，4： | 1 |
| 17 | Yeast hydrolysate attenuates lipopolysaccharide-induced inflammatory responses and intestinal barrier damage in weaned piglets显示文摘Background Intestinal inflammation is the main risk factor causing intestinal barrier dysfunction and lipopolysaccharide(LPS)can trigger inflammatory responses in various eukaryotic species.Yeast hydrolysate(YH)possesses multibiological effects and is received remarkable attention as a functional ingredient for improving growth performance and promoting health in animals.However,there is still inconclusive on the protective effects of dietary YH supplementation on intestinal barrier of piglets.This study was conducted to investigate the attenuate effects of YH supplementation on inflammatory responses and intestinal barrier injury in piglets challenged with LPS.Methods Twenty-four piglets(with an average body weight of 7.42±0.34 kg)weaned at 21 days of age were randomly assigned to one of two dietary treatments(12 replications with one pig per pen):a basal diet or a basal diet containing YH(5 g/kg).On the 22nd d,6 piglets in each treatment were intraperitoneally injected with LPS at 150μg/kg BW,and the others were injected with the same amount of sterile normal saline.Four hours later,blood samples of each piglet were collected and then piglets were euthanized.Results Dietary YH supplementation increased average daily feed intake and average daily gain(P<0.01),decreased the ratio of feed intake to gain of piglets(P sponse,evidenced by the increase o=0.048).Lipopolysaccharide(LPS)injection induced systemic inflammatory ref serum concentrations of haptoglobin(HP),adrenocorticotropic hormone(ACTH),cortisol,and interleukin-1β(IL-1β).Furthermore,LPS challenge resulted in inflammatory intestinal damage,by up-regulation of the protein or mRNA abundances of tumor necrosis factor-α(TNF-α),IL-1β,toll-like receptors 4(TLR4)and phosphor-nuclear factor-κB-p65(p-NFκB-p65)(P<0.01),and down-regulation of the jejunal villus height,the protein and mRNA abundances of zonula occludens-1(ZO-1)and occludin(OCC;P<0.05)in jejunal mucosa.Dietary YH supplementation decreased the impaired effects of ACTH,cortisol,HP,IL-1βand diamine oxidase in serum(P<0.05).Moreover,YH supplementation also up-regulated the jejunal villus height,protein and mRNA abundances of ZO-1 and OCC(P<0.05),down-regulated the mRNA expressions of TNF-αand IL-1βand the protein abundances of TNF-α,IL-1β,TLR4 and p-NFκB-p65 in jejunal mucosa in LPS-challenged pigs(P<0.01).Conclusion Yeast hydrolysate could attenuate inflammatory response and intestinal barrier injury in weaned piglets challenged with LPS,which was associated with the inhibition of TLR4/NF-κB signaling pathway activation. | Runqi Fu Chan Liang Daiwen Chen Gang Tian Ping Zheng Jun He Jie Yu Xiangbing Mao Yuheng Luo Junqiu Luo Bing Yu | 2023 | Journal of Animal Science and Biotechnology2023，14，4： | 0 |
| 18 | General deep learning framework for emissivity engineering显示文摘Wavelength-selective thermal emitters(WS-TEs)have been frequently designed to achieve desired target emissivity spectra,as a typical emissivity engineering,for broad applications such as thermal camouflage,radiative cooling,and gas sensing,etc.However,previous designs require prior knowledge of materials or structures for different applications and the designed WS-TEs usually vary from applications to applications in terms of materials and structures,thus lacking of a general design framework for emissivity engineering across different applications.Moreover,previous designs fail to tackle the simultaneous design of both materials and structures,as they either fix materials to design structures or fix structures to select suitable materials.Herein,we employ the deep Q-learning network algorithm,a reinforcement learning method based on deep learning framework,to design multilayer WS-TEs.To demonstrate the general validity,three WS-TEs are designed for various applications,including thermal camouflage,radiative cooling and gas sensing,which are then fabricated and measured.The merits of the deep Q-learning algorithm include that it can(1)offer a general design framework for WS-TEs beyond one-dimensional multilayer structures;(2)autonomously select suitable materials from a self-built material library and(3)autonomously optimize structural parameters for the target emissivity spectra.The present framework is demonstrated to be feasible and efficient in designing WS-TEs across different applications,and the design parameters are highly scalable in materials,structures,dimensions,and the target functions,offering a general framework for emissivity engineering and paving the way for efficient design of nonlinear optimization problems beyond thermal metamaterials. | Shilv Yu Peng Zhou Wang Xi Zihe Chen Yuheng Deng Xiaobing Luo Wangnan Li Junichiro Shiomi Run Hu | 2023 | Light(Science & Applications)2023，12，12： | 0 |
| 19 | Effect of sialyllactose on growth performance and intestinal epithelium functions in weaned pigs challenged by enterotoxigenic Escherichia Coli显示文摘Background:Sialyllactose(SL)is one of the most abundant oligosaccharides present in porcine breast milk.However,little is known about its effect on growth performance and intestinal health in weaned pigs.This study was conducted to explore the protective effect of SL on intestinal epithelium in weaned pigs upon enterotoxigenic Escherichia coli(ETEC)challenge.Methods:Thirty-two pigs were randomly divided into four treatments.Pigs fed with a basal diet or basal diet containing SL(5.0 g/kg)were orally infused with ETEC or culture medium.Results:SL supplementation elevated the average daily gain(ADG)and feed efficiency in the ETEC-challenged pigs(P<0.05).SL also improved the digestibilities of dry matter(DM),gross energy(GE),and ash in non-challenged pigs(P<0.05).Moreover,SL not only elevated serum concentrations of immunoglobulins(IgA,IgG,and IgM),but also significantly decreased the serum concentrations of inflammatory cytokines(TNF-α,IL-1β,and IL-6)upon ETEC challenge(P<0.05).Interestingly,SL increased the villus height,the ratio of villus height to crypt depth(V:C),and the activities of mucosal sucrase and maltase in the jejunum and ileum(P<0.05).SL also elevated the concentrations of microbial metabolites(e.g.acetic acid,propanoic acid,and butyric acid)and the abundance of Lactobacillus,Bifidobacterium,and Bacillus in the cecum(P<0.05).Importantly,SL significantly elevated the expression levels of jejunal zonula occludins-1(ZO-1),occluding,and fatty acid transport protein-4(FATP4)in the ETEC-challenged pigs(P<0.05).Conclusions:SL can alleviate inflammation and intestinal injury in weaned pigs upon ETEC challenge,which was associated with suppressed secretion of inflammatory cytokines and elevated serum immunoglobulins,as well as improved intestinal epithelium functions and microbiota. | Qiming Duan Daiwen Chen Bing Yu Zhiqing Huang Yuheng Luo Ping Zheng Xiangbing Mao Jie Yu Junqiu Luo Hui Yan Jun He | 2022 | Journal of Animal Science and Biotechnology2022，13，4： | 0 |
| 20 | Effect of dietary canthaxanthin and 25-hydroxycholecalciferol supplementation on the performance of duck breeders under two different vitamin regimens显示文摘Background: Dietary canthaxanthin(CX), 25-hydroxycholecalciferol(25-OH-D3) and vitamins have been widely reported to be involved in productive and reproductive performance of broiler breeders. However, limited information is available for duck breeders. In this study, a total of 1,560 Cherry Valley SM3 duck breeder females and 312 males were used to assess if the addition of CX and 25-OH-D3 could increase the performance of duck breeders under two different dietary vitamin regimens. Four diets were used under a 2 × 2 factorial arrangement with 2 kinds of vitamin premixes(REGULAR and HIGH; HIGH premix had higher levels of all vitamins except K3 than REGULAR premix), and with or without the supplementation of the mixture of CX(6 mg/kg) and 25-OH-D3(0.069 mg/kg). The ducks were fed ad libitum with pelleted diets based on corn-soybean meal from 38 to 77 wk of age.Results: HIGH vitamin premix decreased malondialdehyde(MDA) level(P < 0.001) of egg yolk, increased hatchability of fertile eggs(P = 0.029), increased hatchability of total eggs(P = 0.029), and decreased serum protein carbonyl level(P = 0.037) of breeder males. The mixture of CX and 25-OH-D3 increased serum calcium of breeder females(P = 0.010), decreased the cracked egg rate(P = 0.001), increased the pigmentation of egg yolk(P < 0.001)and male bill(P < 0.001), and decreased MDA level of egg yolk(P < 0.001) and male serum(P = 0.034). Interactive effects were observed in cracked egg rate(P = 0.038), shell thickness(P = 0.011) and serum phosphorus(P = 0.026)of breeder females. HIGH vitamin premix together with the mixture of CX and 25-OH-D3 decreased cracked egg rate and increased shell thickness of duck breeders. Serum phosphorus was decreased in duck breeder females fed REGULAR vitamin premix without the addition of the CX and 25-OH-D3 mixture.Conclusions: Dietary HIGH vitamin premix increased antioxidant status of eggs and breeder males, and increased hatchability. The mixture of CX and 25-OH-D3 enhanced egg shell quality, and promoted pigmentation and antioxidant status of eggs and breeder males. | Zhouzheng Ren Shizhen Jiang Qiufeng Zeng Xuemei Ding Shiping Bai Jianping Wang Yuheng Luo Zhuowei Su Yue Xuan Bing Yao Fernando Cisneros Keying Zhang | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员