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| 1 | Effects of weaning on intestinal longitudinal muscle-myenteric plexus function in piglets显示文摘Weaning piglets usually suffer from severe diarrhea(commonly known as postweaning diarrhea,PWD)along with intestinal motility disorder.Intestinal peristalsis is mainly regulated by the longitudinal muscle-myenteric plexus(LM-MP).To understand the relationship between intestinal LM-MP function and the development of PWD,we compared the intestinal electrical activity,and the transcriptional profile of the LM-MP between 21-day-old piglets(just weaned,n=7)and 24-day-old piglets(suffered the most severe weaning stress,n=7).The results showed that 24-day-old piglets exhibited different degrees of diarrhea.A significant increase in the slow-wave frequency in the ileum and colon was observed in 24-day-old piglets,while c-kit expression in the intestinal LM-MPs was significantly decreased,indicating that PWD caused by elevated slow-wave frequency may be associated with loss of c-kit.The real-time quantitative PCR(RT-q PCR)and enzyme-linked immunosorbent assay(ELISA)showed that intestinal LM-MPs in 24-day-old piglets may undergo inflammation and oxidative stress.Significant increases in 8-hydroxy-2′-deoxyguanosine and decreases in thioredoxin suggest that weaning may lead to DNA damage in the LM-MP of 24-day-old piglets.In addition,activating transcription factor 3 was significantly upregulated,indicating nerve damage in the LM-MP of 24-day-old piglets.The transcriptomic results showed that most of the differentially expressed genes in the ileal LM-MP after weaning were downregulated and closely related to the cell cycle process.Subsequent RT-q PCR analysis showed that the relative expression of p21 was upregulated,while the expression of cyclin A2,cyclin B1,and proliferating cell nuclear antigen was downregulated in the ileal and colonic LM-MP of 24-day-old piglets,suggesting that weaning may inhibit cell proliferation and cause G1/S cell cycle arrest in ileal and colonic LM-MP.In conclusion,weaning may lead to cell cycle arrest by causing DNA damage in the LM-MP,impairing intestinal motility regulation,and ultimately leading to diarrhea in piglets. | Jing Li Fenfen Liu Kaibin Mo Hengjia Ni Yulong Yin | 2024 | Science China(Life Sciences)2024，67，2： | 0 |
| 2 | 复发性流产患者宫腔微环境对早期妊娠的影响显示文摘目的:初步探讨复发性流产(recurrent spontaneous abortion,RSA)患者宫腔微环境对早期妊娠的影响。方法:采集2021年10月—2022年1月就诊于我院拟行清宫术的RSA患者和同期拟行人工流产术的正常早孕女性的宫腔灌洗液,检测其脂多糖(lipopolysaccharide,LPS)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-2(interleukin-2,IL-2)、γ干扰素(interferon-γ,IFN-γ)浓度,并用宫腔灌洗液分别干预子宫内膜细胞和绒毛外滋养细胞,检测子宫内膜细胞TNF-α、IL-2、IFN-γmRNA的表达,以及绒毛外滋养细胞的侵袭迁移能力。结果:RSA患者宫腔灌洗液中LPS、TNF-α、IL-2、IFN-γ的浓度均高于正常早孕女性,差异有统计学意义(均P<0.05)。RSA患者的宫腔灌洗液干预子宫内膜细胞后,TNF-α、IL-2、IFN-γmRNA的表达均较正常早孕女性宫腔灌洗液干预组升高,差异有统计学意义(均P<0.05)。RSA患者宫腔灌洗液干预后的绒毛外滋养细胞的侵袭细胞数目、细胞迁移面积均较正常早孕女性宫腔灌洗液干预后的绒毛外滋养细胞降低,差异有统计学意义(均P<0.05)。结论:RSA患者宫腔呈现微炎症状态,影响绒毛外滋养细胞的侵袭迁移能力,不利于妊娠维持。 | 蔺凯丽 郭洁 张崴 宋殿荣 张继雯 怀其娟 赵琳 | 2023 | 国际妇产科学杂志2023，50，5： | 0 |
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