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| 1 | Distinguishing transgenic from non-transgenic Arabidopsis plants by ~1H NMR-based metabolic fingerprinting显示文摘We have recently reported the construction of an nuclear magnetic resonance (NMR)-based metabonomics study platform, Automics. To examine the application of Automics in transgenic plants, we performed metabolic fingerprinting analysis, i.e., 1H NMR spectroscopy and multivariate analysis, on wild-type and transgenic Arabidopsis. We found that it was possible to distinguish wild-type from four transgenic plants by PLS-DA following application of orthogonal signal correction (OSC). Scores plot following OSC clearly demon-strates significant variation between the transgenic and non-transgenic groups, suggesting that the metabolic changes among wild-type and transgenic lines are possibly associated with transgenic event. We also found that the major contributing metabolites were some specific amino acids (i.e., threonine and alanine), which could correspond to the insertion of the selective marker BAR gene in the transgenic plants. Our data suggests that NMR-based metabonomics is an efficient method to distinguish fingerprinting difference between wild-type and transgenic plants, and can potentially be applied in the bio-safety assessment of transgenic plants. | Yanfei Ren Tao Wang Yufa Peng Bin Xia Li-Jia Qu | 2009 | Journal of Genetics and Genomics2009，36，10： | 7 |
| 2 | Expression of rabbit defensin NP-1 gene in transgenic tobacco plants and its activity against bacterial wilt显示文摘A cDNAderived 200 bp fragment encoding a rabbit defensin NP1 mature peptide was ligated into the GUSless pBI121 to yield pBIC35NP1. Tobacco leaf discs were genetically transformed with an Agrobacterium tumefaciens strain harbouring pBIC35NP1. Northern blot analysis showed that the defensin NP1 expression cassette was normally transcribed in transgenic plants. Resistance tests demonstrated that expression of native defensin NP1 can confer partial resistance to the bacterial wilt pathogen, Ralstonia solanacearum. | Rongzhao Fu Yufa Peng Guangcheng Cao Jiangsheng Ma Caixia Chen Liming Zhang Wenbin Li Yongru Sun | 1998 | Chinese Science Bulletin1998，43，18： | 3 |
| 3 | FAST COMPUTATION OF WIDEBAND RCS USING CHARACTERISTIC BASIS FUNCTION METHOD AND ASYMPTOTIC WAVEFORM EVALUATION TECHNIQUE显示文摘The Asymptotic Waveform Evaluation (AWE) technique is an extrapolation method that provides a reduced-order model of linear system and has already been successfully used to analyze wideband electromagnetic scattering problems. As the number of unknowns increases, the size of Method Of Moments (MOM) impedance matrix grows very rapidly, so it is a prohibitive task for the computation of wideband Radar Cross Section (RCS) from electrically large object or multi-objects using the traditional AWE technique that needs to solve directly matrix inversion. In this paper, an AWE technique based on the Characteristic Basis Function (CBF) method, which can reduce the matrix size to a manageable size for direct matrix inversion, is proposed to analyze electromagnetic scattering from multi-objects over a given frequency band. Numerical examples are presented to il-lustrate the computational accuracy and efficiency of the proposed method. | Sun Yufa Du Yan Shao Yong | 2010 | Journal of Electronics(China)2010，27，4： | 3 |
| 4 | A comparative proteomics approach to detect unintended effects in transgenic Arabidopsis显示文摘Recently, as part of biosafety assessments, unintended effects have been given much attention. In this study, we applied a proteomics approach to elucidate the unintended effects of random T-DNA insertion in transgenic plants. Separated proteins extracted from 12 transgenic Arabidopsis thaliana with different T-DNA insertion sites and from wild-type (ecotype Col-o) were analyzed. In the transgenic plants, 102 significantly altered protein spots were detected, in which 59 were up-regulated and 43 down-regulated. MALDI-TOF MS analysis showed that most of these expression level-altered proteins were involved in energy transfer, oxidative respiration and photosynthesis. However, none of these proteins was a toxic protein or allergen. Using plants with or without cold treatment, a natural environmental stress, as controls, we found that the number of the altered proteins was even less in those transgenic plants than those triggered by the cold treatment, suggesting that the transgenic events had a weaker impact on the plants than the environmental stresses. Interestingly, the phosphinothricin acetyl transferase (PAT), the BAR-encoded protein, was detected in nine out of twelve different T-DNA insertion lines at five different insertion sites. These data suggest that the most significant impact of transgenic events on the host plants is from the transgene itself, i.e., from the predictable intended effects, rather than unintended effects. This study also suggests that the proteomics approach has the potential to detect the unintended effects in transgenic plants. | Yanfei Ren Jun Lv Hua Wang Linchuan Li Yufa Peng Li-Jia Qu | 2009 | Journal of Genetics and Genomics2009，36，10： | 2 |
| 5 | Solidification Behavior of in situ TiB_(2)/Cu Composite Powders during Reactive Gas Atomization显示文摘2wt%TiB_(2)/Cu composite powders were fabricated in situ by reactive gas atomization.The fabricated composite powder exhibits high sphericity,and the powder sizes range from 5μm to 150μm.The morphology of the Cu matrix and the distribution of the TiB2 particles in the composite powders vary with the powder size.The critical transitions of interface morphologies from dendritic-to-cellular and cellular-to-planar interfaces occurs when the composite powder sizes decrease to 34μm and 14μm,respectively.Compared with pure Cu droplets,the composite droplets undergo critical transition of the interface morphologies at a smaller droplet size corresponding to a higher cooling rate because the existence of TiB2 particles can cause instability in the advancing solidification front and heterogeneous nucleation.With decreasing powder size,the extent of the TiB_(2) particle interdendritic segregation decreases as the result of enhanced engulfment of TiB2 particles by the advancing solidification front. | 姜伊辉 ZHANG Mingshu LI Yufa HE Jiangtan CAO Fei 梁淑华 | 2021 | Journal of Wuhan University of Technology(Materials Science)2021，36，2： | 2 |
| 6 | Hollow CoNi alloy submicrospheres consisting of CoNi nanoplatelets:facile synthesis and magnetic properties显示文摘 | Li Hao Liao Jinyun Feng Yufa | | 0，，01： | 1 |
| 7 | Screen of Bacillus thuringiensis toxins for transgenic rice to control Sesamia inferens and Chilo suppressalis显示文摘 | Yulin Gao Yang Hu Qiang Fu Jie Zhang Brenda Oppert Fengxiang Lai Yufa Peng Zhitao Zhang | 2010 | Journal of Invertebrate Pathology2010，，1： | 1 |
| 8 | The failure mechanism of cemented tailings backfill and damage constitutive equation 显示文摘 | Li Shulin Sang Yufa | 1997 | Gold1997，18，1： | 1 |
| 9 | Diblock copolymer healing显示文摘 | Nataliya A. Yufa Jason Li S.J. Sibener | 2009 | Polymer2009，，12： | 1 |
| 10 | Hugan Qingzhi Exerts Anti-Inflammatory Effects in a Rat Model of Nonalcoholic Fatty Liver Disease显示文摘 | WaiJiao Tang Lu Zeng JinJin Yin YuFa Yao LiJuan Feng XiaoRui Yao XiaoMin Sun BenJie Zhou Yoshiji Ohta | 2015 | Evidence-Based Complementary and Alternative Medicine2015，，： | 1 |
| 11 | Development of silk products 显示文摘 | QIU Yufa | 2004 | China Textile & Apparel2004，，9： | 1 |
| 12 | The integral sum number of complete bipartite graph Kr,s 显示文摘 | Wenjie He Yufa Shen Lixin Wang | 2001 | Discrete Math2001，239，： | 1 |
| 13 | Seasonal patterns in the commu- nity of gill monogeneans on wild versus cultured orange-spot- ted grouper,Epinephelus coioides Hamilton,1822 in Daya Bay,South China Sea 显示文摘 | Yufa Luo Tingbao Yang | 2012 | Aquacuhure Research2012，43，： | 1 |
| 14 | A 3D optical metamaterial made by self-assembly显示文摘 | Silvia Vignolini Nataliya A Yufa Pedro S Cunha | 2012 | Adv Ma- ter2012，24，10： | 1 |
| 15 | Review of preferentially selective lithium extraction from spent lithium batteries: Principle and performance显示文摘Lithium,as the lightest and lowest potential metal,is an ideal 'battery metal' and the core strategic metal of the new energy industry revolution.Recovering lithium from spent lithium batteries(LIBs)has become one of the significant approaches to obtaining lithium resources.At present,the lithium extraction being generally placed at the last step of the spent LIBs recovery process has puzzles such as high acid consumption,low Li recovery purity and low recovery efficiency.Selective lithium extraction at the first step of the recovery process can effectively solve those puzzles.Since lithium leaching is a non-spontaneous reaction requiring additional energy to achieve,it is found that these methods can be divided into five ways according to the different types of energy driving the reaction occurring:(ⅰ)electric energy driving lithium extraction;(ⅱ) chemical energy driving lithium extraction;(ⅲ) mechanical energy driving lithium extraction;(ⅳ) thermal energy driving lithium extraction;(ⅴ) other energy driving lithium extraction.Through the analysis of the principle,reaction process and results of recovering lithium methods can provide a few directions for scholars’ subsequent research.It is necessary to speed up the exploration of the principle of these methods.It is expected that this study could provide a reference for the research on the selective lithium extraction. | Zhe Gao Meiting Huang Liming Yang Yufa Feng Yuan Ding Penghui Shao Xubiao Luo | 2023 | Journal of Energy Chemistry2023，，3： | 1 |
| 16 | Downregulation of HOXA1 gene affects small cell lung cancer cell survival and chemoresistance under the regulation of miR-100显示文摘 | Faman Xiao Yifeng Bai Zhenzhu Chen Yufa Li luqiao Luo Jie Huang Jie Yang Hongzhan Liao Linlang Guo | 2014 | European Journal of Cancer2014，，： | 1 |
| 17 | Diblock copolymer healing 显示文摘 | Yufa Nataliya A Li J Sibener S J | 2009 | Polymer2009，50，12： | 1 |
| 18 | Applicat'on oi: Siemens PLC anl its communication technology in steel-making electric arc furnaces and refining furnaces 显示文摘 | Tang Yufa and Wang Yamin | 2001 | microcomputer information2001，17，1： | 1 |
| 19 | Multilevel Characteristic Basis Function Method with ACA for Accelerated Solution of Electrically Large Scattering Problems显示文摘The multilevel characteristic basis function method(MLCBFM)with the adaptive cross approximation(ACA)algorithm for accelerated solution of electrically large scattering problems is studied in this paper.In the conventional MLCBFM based on Foldy-Lax multiple scattering equations,the improvement is only made in the generation of characteristic basis functions(CBFs).However,it does not provide a change in impedance matrix filling and reducing matrix calculation procedure,which is time-consuming.In reality,all the impedance and reduced matrix of each level of the MLCBFM have low-rank property and can be calculated efficiently.Therefore,ACA is used for the efficient generation of two-level CBFs and the fast calculation of reduced matrix in this study.Numerical results are given to demonstrate the accuracy and efficiency of the method. | Li Chenlu Sun Yufa Wang Zhonggen Wang Guohua | 2018 | Transactions of Nanjing University of Aeronautics and Astronautics2018，35，3： | 0 |
| 20 | HAND IN HAND FOR THE MUTUAL FUTURE显示文摘Zhengzhou Yufa (Group) Co., Ltd., the largest white corundum manufacturer in China, the enterprise drafting and revising the national standard of white fused alumina abrasive GB/T 2479-2008, one of top 10 corundum manufacturer in Chinese abrasives industry, has been entitled as'Outstanding Refractories Enterprise in Henan,''TheQuality Credible Enterprise in Henan','Henan Model Enter-prise with Excellent Quality and Excellent Credit', and has passed ISO 9001 Quality Management System Certification. A province level enterprise technology center has been built in Yu fa Group. | Yufa Group | 2019 | China's Refractories2019，28，1： | 0 |
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