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| 1 | Isolation and oral immunogenicity assessment of porcine epidemic diarrhea virus NH-TA2020 strain:One of the predominant strains circulating in China from 2017 to 2021显示文摘Porcine epidemic diarrhea(PED)caused by porcine epidemic diarrhea virus(PEDV)is one of the most devastating diseases in the global pig industry due to its high mortality rate in piglets.Maternal vaccines can effectively enhance the gut-mammary gland-secretory IgA axis to boost lactogenic immunity and passive protection of nursing piglets against PEDV challenge.From 2017 to 2021,we collected 882 diarrhea samples from 303 farms in China to investigate the epidemiology of PEDV.The result showed that about 52.15%(158/303)of the farms were positive for PEDV with an overall detection rate of 63.95%(564/882)of the samples.The S1 fragments of S gene from 104 strains were sequenced for the phylogenetic analysis.A total of 71 PEDV strains(68.27%)sequenced in this study were clustered into the predominant G2c subgroup,while the newly-defined G2d strains(9.62%)were identified in three provinces of China.The NH-TA2020 strain of G2c subgroup was isolated and cultured,and its infection to piglets caused watery diarrhea within 24 h,indicating its strong pathogenicity.Oral administration of NH-TA2020 strain to pregnant gilts stimulated high levels of IgA antibody in colostrum.The piglets fed by the gilts above were challenged with NH-TA2020 strain or CH-HeB-RY-2020 strain from G2d subgroup,and the clinical symptoms and virus shedding were significantly reduced compared to the mock group.Our findings suggest that G2c subgroup is the predominant branch circulating in China from 2017 to 2021.Oral administration of NH-TA2020 enhances maternal IgA and lactogenic immune responses,which confer protection against the homologous and emerging G2d PEDV strains challenges in neonates. | Xiaowen Li Yang Li Jiapei Huang Yali Yao Wenying Zhao Yunjing Zhang Jie Qing Jing Ren Zhong Yan Zewei Wang Xiaofang Hu Duli Kang Hongqiang Liu Zhichun Yan | 2022 | Virologica Sinica2022，37，5： | 6 |
| 2 | Copy number and zygosity determination of transgenic rapeseed by droplet digital PCR显示文摘Establishing an accurate and rapid method for copy number and zygosity determination can accelerate genetic engineering research process. In this study, droplet digital PCR (ddPCR), an emerging DNA absolute quantification technology, was used to identify single-copy homozygous transgenic lines from a batch of T0 transgenic rapeseed harboring 11 exogenous elements. Copy number of exogenous gene was evaluated in T0 generation based on calculated ratio between transgene and reference CruA gene, single-copy transformants were selected for selfing followed by subsequent zygosity analysis. Single-copy homozygous transgenic plants were successfully screened out in T1 generation by ddPCR.Segregation analysis with T2 seedlings verified that identification results of ddPCR were accurate and reliable. This study provides a novel rapid and accurate method for copy number and zygosity determination in transgenic rapeseed which overcomes disadvantages of traditional Southern analysis and recently developed real-time quantitative method. | Yuhua Wu Jun Li Xiaying Li Jingang Liang Yunjing Li Xinhua Zeng Gang Wu | 2017 | Oil Crop Science2017，2，2： | 4 |
| 3 | Photoluminescence and electrical properties of Er3+-dopedNa0.5Bi4.5Ti4O15-Bi4Ti3O12 inter-growth ferroelectric ceramics显示文摘 | Yalin JIANG Xiangping JIANG Chao CHEN Yunjing CHEN Xingan JIANG Na TU | 2017 | Frontiers of Materials Science2017，11，1： | 3 |
| 4 | Effects of Cr2O3 doping on the microstructure and electrical properties of (Ba,Ca)(Zr,Ti)O3 lead-free ceramics显示文摘 | Xiang XlA Xiangping JIANG Chao CHEN Xingan JIANG Na TU Yunjing CHEN | 2016 | Frontiers of Materials Science2016，10，2： | 3 |
| 5 | The CRL3^(BTBD9) E3 ubiquitin ligase complex targets TNFAIP1 for degradation to suppress cancer cell migration显示文摘Tumor necrosis factor alpha-induced protein 1(TNFAIP1)modulates a plethora of important biological processes,including tumorigenesis and cancer cell migration.However,the regulatory mechanism of TNFAIP1 degradation remains largely elusive.In the present study,with a label-free quantitative proteomic approach,TNFAIP1 was identified as a novel ubiquitin target of the Cullin-RING E3 ubiquitin ligase(CRL)complex.More importantly,Cul3-ROC1(CRL3),a subfamily of CRLs,was identified to specifically interact with TNFAIP1 and promote its polyubiquitination and degradation.Mechanistically,BTBD9,a specific adaptor component of CRL3 complex,was further defined to bind and promote the ubiquitination and degradation of TNFAIP1 in cells.As such,downregulation of BTBD9 promoted lung cancer cell migration by upregulating the expression of TNFAIP1,whereas TNFAIP1 deletion abrogated this effect.Finally,bioinformatics and clinical sample analyses revealed that BTBD9 was downregulated while TNFAIP1 was overexpressed in human lung cancer,which was associated with poor overall survival of patients.Taken together,these findings reveal a previously unrecognized mechanism by which the CRL3^(BTBD9) ubiquitin ligase controls TNFAIP1 degradation to regulate cancer cell migration. | Lihui Li Wenjuan Zhang Yue Liu Xiaojun Liu Lili Cai Jihui Kang Yunjing Zhang Wenlian Chen Changsheng Dong Yanmei Zhang Mingsong Wang Wenyi Wei Lijun Jia | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 2 |
| 6 | The preparations and applications of microe-mulsion显示文摘 | YunJ(恽杰) | | 北方药学0，8，8： | 1 |
| 7 | Study on acridine orange dimer as a new fluorescent probe for the determination of protein 显示文摘 | Luo Yunjing Shen Hanxi | 1999 | Anal Commun1999，36，： | 1 |
| 8 | The Zuckerkandl's tubercle : a useful anatomical landmark for detecting both the recurrent laryngeal nerve and the superior parathyroid during thyroid surgery显示文摘 | YUNJ S LEE Y S JUNG J J | 2008 | Endocr J2008，55，5： | 1 |
| 9 | Recent advances in engineered nanomaterials for acute kidney injury theranostics显示文摘Acute kidney injury(AKI),has become the focus of increasing attention due to its high risk of death.The early diagnosis and treatment of AKI significantly reduce the risk of renal tissue damage and kidney dysfunction.However,the efficient early diagnosis and treatment approach for AKI remains a challenge.AKI screening via precise nanomaterial theranostics is a new alternative approach.This study summarizes the recent advances in functional nanomaterials in the early detection and treatment of AKI.The challenges and problems in the use of nanomaterials for AKI in clinical applications are also discussed.It is anticipated that highlighting these new advances will lay the foundation for further translational research on the promising application of nanomaterials for AKI. | Lefeng Wang Yunjing Zhang Yangyang Li Jianghua Chen Weiqiang Lin | 2021 | Nano Research2021，14，4： | 1 |
| 10 | Tuning the selectivity of polymeric ionic liquid sorbent coatings for the extraction of polycyclic aromatic hydrocarbons using solid-phase microextraction显示文摘 | Yunjing Meng Jared L. Anderson | 2010 | Journal of Chromatography A2010，，40： | 1 |
| 11 | Error concealment of multi-view video sequences using inter-view and intra-view correlations显示文摘 | Kwanwoong Song Taeyoung Chung Yunje Oh Chang-Su Kim | 2009 | Journal of Visual Communication and Image Representation2009，，4： | 1 |
| 12 | MIR205HG facilitates carcinogenesis of lung squamous cell carcinoma in vitro revealed by long noncoding RNA profiling显示文摘As a subtype of non-small-cell lung cancer,lung squamous cell carcinoma(LUSC)accounts for one-fifth of all lung cancers.Unfortunately,no specific targetable aberration has yet been identified.Hence,it is of huge urgency and potential to identify aberrantly regulated genes in LUSC.Here,five pairs of LUSC samples and their corresponding adjacent tissues were subject to whole transcriptome sequencing.Our results showed that CTD-2562J17.6 and FENDRR were significantly downregulated while MIR205HG,LNC_000378,RP11-116G8.5,RP3-523K23.2,and RP5_968D22.1 were significantly upregulated in all five LUSC samples.Importantly,MIR205HG was upregulated in LUSC clinical samples as well as in LUSC cell lines.Interestingly,our results demonstrated that the expression level of MIR205HG is positively correlated with the malignancy.In addition,MIR205HG is required for LUSC cell growth and cell migration.Most importantly,our results showed that MIR205HG prohibits LUSC apoptosis via regulating Bcl-2 and Bax.Taken together,our data shed lights on the IncRNA regulatory nexus that controls the carcinogenesis of LUSC and provided potential novel diagnostic markers and therapeutic targets for LUSC. | Yan Chang Xinying Xue Chunsun Li Wei Zhao Yongfu Ma Fei Xu Zhen Wu Yu Dai Yunjing Li Yang Liu Liang’an Chen | 2020 | Acta Biochimica et Biophysica Sinica2020，52，4： | 1 |
| 13 | Transcriptional and post‑transcriptional regulation of RNAi‑related gene expression during plant‑virus interactions显示文摘As sessile organisms,plants encounter diverse invasions from pathogens including viruses.To survive and thrive,plants have evolved multilayered defense mechanisms to combat virus infection.RNAi,also known as RNA silencing,is an across-kingdom innate immunity and gene regulatory machinery.Molecular framework and crucial roles of RNAi in antiviral defense have been well-characterized.However,it is largely unknown that how RNAi is transcriptionally regulated to initiate,maintain and enhance cellular silencing under normal or stress conditions.Recently,insights into the transcriptional and post-transcriptional regulation of RNAi-related genes in different physiological processes have been emerging.In this review,we integrate these new findings to provide updated views on how plants modulate RNAi machinery at the(post-)transcriptional level to respond to virus infection. | Qian Gong Yunjing Wang Zhenhui Jin Yiguo Hong Yule Liu | 2022 | Stress Biology2022，2，1： | 1 |
| 14 | Downregulationofcell-freemiR-198asadiagnosticbiomarkerforlungadenocarcinoma-associatedmalignantpleuraleffusion显示文摘 | HanHS YunJ LimSN etal | 2013 | IntJCancer2013，133，3： | 1 |
| 15 | 查看详情显示文摘 | YunJ Isayev A I KimSH Tapale M | | 0，，2： | 1 |
| 16 | 查看详情显示文摘 | YunJ IsayevAI | | 0，，10： | 1 |
| 17 | 查看详情显示文摘 | YunJ IsayevAI | | 0，，1： | 1 |
| 18 | Hepatoprotective effects of berberine on liver fibrosis via activation of AMP-activated protein kinase显示文摘 | Jing Li Yue Pan Mujie Kan Xuanang Xiao Yunjing Wang Fengying Guan Xuewen Zhang Li Chen | 2014 | Life Sciences2014，，1： | 1 |
| 19 | Microsphere-assisted,nanospot,non-destructive metrology for semiconductor devices显示文摘As smaller structures are being increasingly adopted in the semiconductor industry,the performance of memory and logic devices is being continuously improved with innovative 3D integration schemes as well as shrinking and stacking strategies.Owing to the increasing complexity of the design architectures,optical metrology techniques including spectroscopic ellipsometry(SE)and reflectometry have been widely used for efficient process development and yield ramp-up due to the capability of 3D structure measurements.However,there has been an increasing demand for a significant reduction in the physical spot diameter used in the SE technique;the spot diameter should be at least 10 times smaller than the cell dimension(~30×40μm2)of typical dynamic random-access memory to be able to measure in-cell critical dimension(CD)variations.To this end,this study demonstrates a novel spectrum measurement system that utilizes the microsphere-assisted super-resolution effect,achieving extremely small spot spectral metrology by reducing the spot diameter to~210 nm,while maintaining a sufficiently high signal-to-noise ratio.In addition,a geometric model is introduced for the microsphere-based spectral metrology system that can calculate the virtual image plane magnification and depth of focus,providing the optimal distance between the objective lens,microsphere,and sample to achieve the best possible imaging quality.The proof of concept was fully verified through both simulations and experiments for various samples.Thus,owing to its ultra-small spot metrology capability,this technique has great potential for solving the current metrology challenge of monitoring in-cell CD variations in advanced logic and memory devices. | Soonyang Kwon Jangryul Park Kwangrak Kim Yunje Cho Myungjun Lee | 2022 | Light(Science & Applications)2022，11，2： | 1 |
| 20 | Grating-assisted polarizationoptical time-domain reflectometry for distributed fiber-op-tic sensing 显示文摘 | Wang Yunjing Wang Anbo | 2007 | Optics Letters2007，32，4： | 1 |
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