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| 1 | Evolution and molecular epidemiology of foot-and-mouth disease virus in China显示文摘Foot-and-mouth disease (FMD),caused by foot-and-mouth disease virus (FMDV),is considered one of the most important viral diseases of cloven-hoofed animals,causing severe economic losses in affected regions of the world.Three serotypes (A,O,and Asia 1) of FMDV have been identified in China since 1958.In addition,the occurrence of novel subtypes within these serotypes has made the epidemiology of FMDV more complicated over the last few years.In this review,we summarize the history and the current epidemiological situation in China,genetic diversity (e.g.,quasispecies dynamics,antigenic heterogeneity,and functional constraints),intertypic recombination,and the evidence for positive selection of different FMDV serotypes.We also assess these genetic data to understand the origin,evolution,and transmission of FMDV,the findings of which may be useful in developing control measures for future epidemics. | BAI XingWen LI PingHua BAO HuiFang LIU ZaiXin LI Dong LU ZengJun CAO YiMei SHANG YouJun SHAO JunJun CHANG HuiYun LUO JianXun LIU XiangTao | 2011 | Chinese Science Bulletin2011，56，21： | 7 |
| 2 | Recovery of infectious foot-and-mouth disease virus from full-length genomic cDNA clones using an RNA polymerase I system显示文摘prototypic foot-and-mouth 疾病病毒(FMDV ) 超过一个世纪以前被显示出是能够引起 FMD 的第一个可滤过的代理人,并且它为学习 Aphthovirus 分子的生物学的基本原则担任了一个重要模型。然而,复杂结构和 FMDV 的 antigenic 差异在由反向的基因技术操作传染病毒形成了一个主要障碍到尝试。这里,我们从 cDNAs 报导传染 FMDV 的恢复基于一在 vivo RNA 有效聚合酶吗?(pol ?) 抄写系统。从 pol 的全身的病毒的染色体的细胞内部的抄写 ? 基于的向量导致了传染病毒显示的有效形成一个基因标记。与野类型的病毒相比,许多 genomic mRNA 和病毒的抗原的提高的表示层次在整个在抄写,复制,和翻译层次的这个导出 cDNA 的病毒的生命周期的功能亢进是指示的。这里描述的技术能是为学习 FMDV 复杂传染特征的一个极其珍贵的分子的生物学工具。它是为学习 FMDV 功能的 genomics 的一个操作平台,致病力和变化的分子的机制,并且向一支遗传上设计的疫苗的前景为病毒的怪物的开发打一个稳固的基础。 | Yanyan Chang Haixue Zheng Youjun Shang Ye Jin Guangxiang Wang Xiaoyan Shen Xiangtao Liu | 2009 | Acta Biochimica et Biophysica Sinica2009，41，12： | 3 |
| 3 | Development of a hamster kidney cell line expressing stably T7 RNA polymerase using retroviral gene transfer technology for efficient rescue of infectious foot-and-mouth disease virus显示文摘 | Haixue Zheng Hong Tian Ye Jin Jinyan Wu Youjun Shang Shuanghui Yin Xiangtao Liu QingGe Xie | 2008 | Journal of Virological Methods2008，，1： | 1 |
| 4 | Diagnosis and phylogenetic analysis of Orf virus from goats in China:a case report显示文摘 | Keshan Zhang Zhongxin Lu Youjun Shang | | 0，，： | 1 |
| 5 | Complete nucleotide sequence of a Chinese serotype Asia1 vaccine strain of foot-and-mouth disease virus显示文摘 | Junzheng Du Huiyun Chang Guozheng Cong Junjun Shao Tong Lin Youjun Shang Zaixin Liu Xiangtao Liu Xuepeng Cai Qingge Xie | 2007 | Virus Genes2007，，3： | 1 |
| 6 | Rapid Detection Co-infections of Classical Swine Fever Virus and Porcine Reproductive and Respiratory Syndrome Virus by One-step Multiplex RT-PCR显示文摘Classical swine fever virus(CSFV) and porcine reproductive and respiratory syndrome virus(PRRSV) have caused immense economic loss in the pig industry and are considered to be the two most important infectious diseases of pigs in the world. A multiplex reverse transcription polymerase chain reaction(multiplex RT-PCR) was developed for CSFV and PRRSV co-infections or infections,respectively. A set of two pairs of primer was designed based on the sequence of nonstructural protein NS54B of CSFV and ORF7 gene of PRRSV. The diagnostic accuracy of multiplex RT-PCR assay was evaluated by using 56 field clinical samples by multiplex RT-PCR,single RT-PCR and sequence analysis;and the specificity of multiplex PCR was verified by using constructed plasmids containing the specific viral target fragments of PRRSV and CSFV,respectively. The results indicated that this assay could reliably differentiate PRRSV and CSFV in co-infection samples. The multiplex RT-PCR developed in this study might provide a new avenue to the rapid the detection of CSFV and PRRSV in one reaction. | TIAN Hong WU Jinyan YAN Chen SHANG Youjun YIN Shuanghui LIU Xiangtao | 2011 | Journal of Northeast Agricultural University(English Edition)2011，18，4： | 1 |
| 7 | The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus显示文摘 | Hong Tian JingYan Wu YouJun Shang | 2010 | Virology Journal2010，，7： | 1 |
| 8 | Eukaryotic Expression and Activity Analysis of Capsid Gene of Swine Vesicular Disease Virus HK/70显示文摘The capsid protein precursor(P1),which plays a major role for the generation of polypeptides of swine vesicular disease virus(SVDV),was cloned from SVDV HK/70 strain into the retroviral vector pBABE puro and expressed in the mammalian cell line PK15 through the retroviral expression system.The activity of recombinant protein to induce immune response was evaluated in guinea pigs.IFA and Western Blot were used to detect the recombinant protein expression.The results showed that the recombinant protein could be recognized by SVDV positive serum,and animal test showed SVDV-specifi c antibodies.All of those results indicate that a retroviral-based vaccine carrying the capsid protein precursor(P1) of SVD is able to be expressed in the eukaryotic cell and elicites strong SVDV-specifi c immune responses in guinea pigs. | TIAN Hong WU Jingyan SHANG Youjun YING Shuanghui ZHENG Haixue LIU Xiangtao XIE Qingge | 2009 | Journal of Northeast Agricultural University(English Edition)2009，16，4： | 0 |
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