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| 1 | Expression in Escherichia coli of Three Different Soybean Late Embryogenesis Abundant (LEA) Genes to Investigate Enhanced Stress Tolerance显示文摘In order to identify the function of late embryogenesis abundant (LEA) genes, in vitro functional analyses were perfo rmed using an Escherichia coli heterologous expression system. Three soybean late embryogenesis abundant (LEA) genes, PM11 (GenBank accession No. AF004805; group 1), PM30 (AF1 17884; group 3), and ZLDE-2 (AY351918; group 2), were cloned and expressed in a pET-28a system.The gene products were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and identified by mass spectrometry. E. coli cells containing the recombinant plasmids or empty vector as controls were treated by salt and low temperature stress. Compared with control cells, the E. coli cells expressing either PM11 or PM30 showed a shorter lag period and improved growth when transferred to LB (Luria-Bertani) liquid media containing 800 mmol/L NaCl or 700 mmol/L KCl or after 4 ℃ treatment. E. coli cells expressing ZLDE-2 did not show obvious growth improvement both in either high KCl medium or after 4 ℃ treatment. The results indicate that the E. coli expression system is a simple, useful method to identify the functions of some stress-tolerant genes from plants. | YingLAN DanCAI Yi-ZhiZHENG | 2005 | Journal of Integrative Plant Biology2005,47,5: | 23 |
| 2 | Cationic nanocarriers induce cell necrosis through impairment of Na^+/K^+-ATPase and cause subsequent inflammatory response显示文摘有积极表面费用的 Nanocarriers 以他们限制了他们的临床的应用的毒性著名。位于他们的毒性下面的机制例如煽动性的反应的正式就职,仍然保持大部分未知。在我们发现了 cationic nanocarriers 的那注射的现在的学习,包括 cationic liposomes, PEI,和 chitosan,导致了坏死的房间的快速的外观。cationic nanocarriers 导致的房间坏死依赖于他们的积极表面费用,但是不要求 RIP1 和 Mlkl。相反,细胞内部的 Na + 超载被发现伴随房间死亡。在文化媒介或有 Na +/K+-ATPase 阳离子绑定地点禁止者 ouabain 的房间的预告的处理的 Na + 的弄空,从房间坏死的保护的房间。而且,有 cationic nanocarriers 的处理在 vitro 并且在 vivo 禁止了 Na +/K+-ATPase 活动。计算模拟证明 cationic 搬运人能与 Na +/K+-ATPase 的阳离子绑定地点交往。有 ouabain 的小剂量的老鼠 pretreated 在 cationic nanocarriers 的致命的剂量的注射以后显示出改进幸存。进一步的分析建议 cationic nanocarriers 和 mitochondrial DNA 的产生的漏导致的那房间坏死能在 vivo 触发严重发炎,它被包含 TLR9 并且 MyD88 发信号的一条小径调停。一起拿,我们的结果揭示由此的新奇机制 cationic nanocarriers 通过和 mitochondrial 的随后的暴露的和 Na +/K+-ATPase, 的相互作用导致尖锐房间坏死是的联系损坏的分子的模式调停的一个关键事件煽动性的回答。我们的学习为评估 nanocarriers 的 biocompatibility 并且为药交货设计更好、更安全的有重要含意。 | Xiawei Wei Bin Shao Zhiyao He Tinghong Ye Min Luo Yaxiong Sang Xiao Liang Wei Wang Shuntao Luo Shengyong Yang Shuang Zhang Changyang Gong Maling Gou Hongxing Deng Yinglan Zhao Hanshuo Yang Senyi Deng Chengjian Zhao Li Yang Zhiyong Qian Jiong Li Xun Sun Jiahuai Han Chengyu Jiang Min Wu Zhirong Zhang | 2015 | Cell Research2015,25,2: | 21 |
| 3 | Dual origins of Xiaoqinling gold-bearing quartz veins:Fluid inclusion evidence显示文摘Fluid inclusion research in Archean metamorphic rocks, Yanshanian granite and gold-bearing quartz veins shows that regional metamorphic fluids are high temperature and high saline, Yanshanian post-granitic fluids are CO2-bearing low saline, and ore-forming fluids are also CO2-rich low saline. In gold-barren/free parts of quartz veins in gold deposits, daughter mineral-bearing high saline inclusions related with metamorphic fluids remained. This proclaims quartz veins hosting gold ores might have been formed in early metamorphism, and overlapped or mineralized by late Yanshanian gold-bearing fluids. | Hongrui Fan Yihan Xie Rui Zhao Yinglan Wang | 2000 | Chinese Science Bulletin2000,45,15: | 17 |
| 4 | Synthesis of a novel ternary HA/Fe-Mn oxides-loaded biochar composite and its application in cadmium(Ⅱ) and arsenic(Ⅴ) adsorption显示文摘Cadmium (Cd) and arsenic (As) are two of the most toxic elements.However,the chemical behaviors of these two elements are different,making it challenging to utilize a single adsorbent with high adsorption capacity for both Cd(Ⅱ) and As(Ⅴ) removal.To solve this problem,we synthesized HA/Fe-Mn oxides-loaded biochar (HFMB),a novel ternary material,to perform this task,wherein scanning electron microscopy (SEM) combined with EDS (SEM-EDS) was used to characterize its morphological and physicochemical properties.The maximum adsorption capacity of HFMB was 67.11 mg/g for Cd(Ⅱ) and 35.59 mg/g for As(Ⅴ),which is much higher compared to pristine biochar (11.06 mg/g,0 mg/g for Cd(Ⅱ) and As(Ⅴ),respectively).The adsorption characteristics were investigated by adsorption kinetics and the effects of the ionic strength and pH of solutions.X-ray photoelectron spectroscopy (XPS) and Fourier-transform infrared spectroscopy (FT-IR) revealed that chelation and deposition were the adsorption mechanisms that bound Cd(Ⅱ) to HFMB,while ligand exchange was the adsorption mechanism that bound As(Ⅴ). | Jianhua Guo Changzhou Yan Zhuanxi Luo Hongda Fang Shugang Hu Yinglan Cao | 2019 | Journal of Environmental Sciences2019,31,11: | 10 |
| 5 | Specifically Binding of L-ficolin to N-glycans of HCV Envelope Glycoproteins E1 and E2 Leads to Complement Activation显示文摘 | Jun Liu Mohammed A.M. Ali Yinghua Shi Yinglan Zhao Fenglin Luo Jin Yu Tian Xiang Jie Tang Dongqing Li Quan Hu Wenzhe Ho Xiaolian Zhang | 2009 | Cellular & Molecular Immunology2009,6,4: | 8 |
| 6 | Intercalibration of international and domestic ^(40)Ar/^(39)Ar dating standards显示文摘Four international standards, Ga1550, MMhb-1, Lp-6, Bern 4M, and one domestic standard BT-1 have been intercalibrated. The repeated measurements on MMhb-1 with different mass demonstrate that MMhb-1 is inhomogeneous in age and its average age is 519.8 Ma. The results of Bern 4M and Lp-6 reflect that they have an invariable value of 40Ar*/39Ark (F) and the ages we obtained are consensus with their K-Ar age: Lp-6=127.7Ma; Bern 4M=18.2 Ma. Analyses of BT-1 age spectra, Ca/K and Cl/K spectra as well as inverse isochrons indicate that the sample is homogeneous and invariable and keeps close chemically, with its trapped argon isotope composition close to the at- mosphere. The dating results show that age values are reproducible and steady, total fusion age, step-heating age, plateau age and isochron age are in accord with each other within the error range (2σ). Therefore, we recommend 28.7 Ma as the calibrated age of BT-1. We also discuss the variation in neutron flux gradients of Beijing 49-2 reactor. It was found that the neutron flux gradient varies considerably, and more monitors (standard samples) are needed to fix the trend of variation. The coefficient of the 49-2 reactor that transfers the ratio of production rate of 37ArCa/39ArK into Ca/K ratio is 1.78. This is different from that reported earlier, 2.0, which may be caused by the reconstruction of the reactor. | WANG Fei HE Huaiyu ZHU Rixiang SANG Haiqing WANG Yinglan YANG Liekun | 2006 | Science China Earth Sciences2006,49,5: | 6 |
| 7 | Study on regional material flow analysis of Chengyang District in Qingdao显示文摘Base on the framework of material flow analysis (MFA) proposed by Eurostat, this paper collected relateddata and conducted a regional MFA for the Chengyang District in Qingdao. The indicators such as Direct Material Input(DMI), Direct Material Consumption (DMC) and Exports are calculated or estimated for the period of year 1995through 2004. Several derivable indicators defining direct material productivity and material intensity are also evaluatedand some valuable conclusions were drawn. DMI in absolute number increased from about 1645.9 kilotons in 1995 toabout 8052.5 kilotons in 2004. Imports contribute to about 47 to 69% of DMI, and the biggest component of imports isfossil fuel that approximately accounts for 50% of imports. DMI per capita of Chengyang District increased rapidly andreached 17.4 tons in 2004. Compared with other five economies studied by WRI, material consumption intensity ofChengyang District is significantly lower than developed countries, which indicates that Chengyang District is still in adevelopment stage. The direct material productivity (actual GDP per DMI) increased 59.1% from 1995 to 2004 and hasbeen higher than either of studies in China, which indicates efficiency of resources utilization has improved obviously inChengyang District. But there was significant increase in material input in the last decade, which shows that rapidincrease of economy is highly dependent on requirement and consumption of natural resources. Therefore, in order topromote the sustainability, it is essential to develop circular economy and improve utilization efficiency of resources. | Zhou Zhenfeng Sun Yinglan Wang Jun | 2006 | Ecological Economy2006,2,1: | 5 |
| 8 | Laser step-heating ^(40)Ar/^(39)Ar dating on young volcanic rocks显示文摘An attempt was made to use CO_2 laser step-heating method to date the late Pleistocene basaltic groundmass (DF-2) from Tengchong volcanic field. Among the fourteen heating steps, ten define a good inverse isochron (MSWD = 1.4) with an age of 32.2 ± 7.1 ka (2 σ).The inverse isochron also shows that the initial argon isotopic ratio is 297.1±2.0 (2σ) which is the same as the atmospheric argon at the 2σ error level. Study indicates that it is a useful means to date young volcanic groundmass with low K content by using CO_2 laser step-heating ^(40)Ar/^(39)Ar method. | WANG Fei HE Huaiyu ZHU Rixiang YANG Liekun SANG Haiqing WANG Yinglan | 2006 | Chinese Science Bulletin2006,51,23: | 4 |
| 9 | Stable isotope geochemical characteristics of dissolved inorganic carbon in the Jiulong River Estuary, Fujian Province, China显示文摘The isotopic composition of dissolved inorganic carbon(DIC) in estuarine environments has been studied for its significant role in determining the isotopic composition of inorganic/organic matter and its applications to the study of various natural processes. In this paper, based on the stable isotope geochemical characteristics of dissolved inorganic carbon in the Jiulong River Estuary, the following conclusions are drawn:(1) δ13CDIC values are mainly controlled by the mixing ratio of fresh water and sea water;(2) δ13Cphytoplankton values are linearly related to the δ13CDIC values;(3) δ13CPOM values for the Jiulong River Estuary are affected by anthropogenic pollution significantly; and(4) the comprehensive analysis of δ13Cphytoplankton, δ13CPOM and δ13CDIC shows that along with increasing salinity, the proportion of POM derived from the degradation of phytoplanktons gradually increases. | LIU Qiming WU Qiong CAO Yinglan LIN Jinmei JIAO Yupei | 2014 | Chinese Journal Of Geochemistry2014,33,2: | 2 |
| 10 | Chinese nursing students’ HIV/AIDS knowledge, attitudes, and practice intentions显示文摘 | Yinglan Li Craig S. Scott Li Li | 2008 | Applied Nursing Research2008,,3: | 1 |
| 11 | Dangshen{Codonopsis pilosula) activates IGF-I and FGF-2 pathways to induceproliferation and migration effects in RSC96 schwann cells显示文摘 | CHEN Hsientung TSAI Yinglan CHEN Yuehsheng | 2010 | TheAmerican Journal of Chinese Medicine2010,38,2: | 1 |
| 12 | Investigation of oxidation resistance of magnetic power coated with silicon显示文摘 | ZHANG Yinglan | 1997 | Journal of Magnetism and Magnetic Materials1997,171,: | 1 |
| 13 | Buflomedil treatment of vertebral - basilar artery insufficiency vertigo curative effect observation 显示文摘 | Denny li li hong Guo Yinglan | 2006 | Journal of hebei medicine2006,,3: | 1 |
| 14 | Temperature analysis and characteristics of highly strained InGaAsGaAsP-GaAs quantum well lasers显示文摘 | Chang Yinglan Tetsuysa Takeuchi | 2002 | IEEE Transactions on Quantum Electronics2002,38,6: | 1 |
| 15 | 拟枝状双胞霉一新种(英文) | Guo Yinglan (Systematic Mycology & Lichenology Laboratory,Institute of Microbiology, Academia Sinica,Beijing 100080) | 1999 | 安徽农业大学学报1999,26,3: | 1 |
| 16 | Management of marine cage aquaculture: environmental carrying capacity method based on dry feed conversion rate 显示文摘 | Cai Huiwen Sun Yinglan | 2007 | Environmental Science and Pollution Research2007,14,7: | 1 |
| 17 | Stem Cell-Like Circulating Tumor Cells Indicate Poor Prognosis in Gastric Cancer显示文摘 | Man Li Baogang Zhang Zhiguang Zhang Xia Liu Xiangjuan Qi Jianqiu Zhao Yong Jiang Haoyu Zhai Yinglan Ji Dan Luo Shiwu Zhang | 2014 | BioMed Research International2014,,: | 1 |
| 18 | Combination synchronization of three classic chaotic systems using active backstepping design 显示文摘 | Luo Runzi Wang Yinglan Deng Shucheng | 2011 | Chaos2011,21,04: | 1 |
| 19 | Increasing stiffness promotes pulmonary retention of ligand-directed dexamethasone-loaded nanoparticle for enhanced acute lung inflammation therapy显示文摘Inhaled nanoparticles(NPs)need to penetrate the bronchial mucosa to deliver drug payloads deeply in the lung for amplified local therapy.However,the bronchial mucociliary barrier eliminates NPs rapidly,which considerably limits their mucosal penetration.In this study,we find that surface ligand modification and stiffness adjustment of NPs contribute to the significantly enhanced bronchial mucosal absorption and pulmonary retention of inhaled drugs.We utilize neonatal Fc receptor ligand(FcBP)to modify the rationally designed low stiffness NPs(Soft-NP)and high stiffness NPs(Stiff-NP)to target bronchial mucosa.In an acute lung inflammation rat model,after intranasal administration with dexamethasone-loaded NPs,Stiff-NP endowed with FcBP displays superior therapeutic effects.The in vitro data demonstrate that the promotion effect of FcBP to bronchial mucosal absorption of Stiff-NP dominates over Soft-NP.This could be attributed to the higher affinity between ligand-receptor when incorporating FcBP on the Stiff-NP surface.Meanwhile,high stiffness modulates more actin filaments aggregation to mediate endocytosis,along with strengthened Ca2+signal to enhance exocytosis.Conclusively,we highlight that FcBP-modified NPs with higher stiffness would be a potential pulmonary drug delivery system. | Yinglan Yu Shujie Li Yuan Yao Xinran Shen Lian Li Yuan Huang | 2023 | Bioactive Materials2023,,2: | 0 |
| 20 | new Heights in Chinese Acrobatics——A Report on the Fourth National Acrobatics Contest显示文摘FROM September 15 to 23,1995 China‘s Fourth NationalAcrobatics Contest was held inshenyang in Liaoning Province.Thecompetition held every four years wasjointly sponsored by the Ministry ofCulfure and the municipal governmentof the city.It aimed at carrying forwardChina’s outstanding national culture,developing acrobatics,and presentingexcellent acts so as to keep pacewith he international level of the art.Compared with the previous | Lin Yinglan | 1996 | China & The World Cultural Exchange1996,62,1: | 0 |