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| 1 | Techniques for detecting protein-protein interactions in living cells:principles, limitations, and recent progress显示文摘Detecting protein-protein interactions(PPIs) provides fundamental information for understanding biochemical processes such as the transduction of signals from one cellular location to another; however, traditional biochemical techniques cannot provide sufficient spatio-temporal information to elucidate these molecular interactions in living cells. Over the past decade, several new techniques have enabled the identification and characterization of PPIs. In this review, we summarize three main techniques for detecting PPIs in vivo, focusing on their basic principles and applications in biological studies. We place a special emphasis on their advantages and limitations, and, in particular, we introduced some uncommon new techniques, such as single-molecule FRET(smFRET), FRET-fluorescence lifetime imaging microscopy(FRET-FLIM), cytoskeleton-based assay for protein-protein interaction(CAPPI) and single-molecule protein proximity index(smPPI), highlighting recent improvements to the established techniques. We hope that this review will provide a valuable reference to enable researchers to select the most appropriate technique for detecting PPIs. | Yaning Cui Xi Zhang Meng Yu Yingfang Zhu Jingjing Xing Jinxing Lin | 2019 | Science China(Life Sciences)2019，62，5： | 9 |
| 2 | Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes显示文摘Although advances in protein assembly preparation have provided a new platform for drug delivery during tissue engineering,achieving long-term controlled exosome delivery remains a significant challenge.Diffusion-dominated exosome release using protein hydrogels results in burst release of exosomes.Here,a fibroin-based cryo-sponge was developed to provide controlled exosome release.Fibroin chains can self-assemble into silk I structures under ice-cold conditions when annealed above the glass transition temperature.Exosome release is enzyme-responsive,with rates primarily determined by enzymatic degradation of the scaffolds.In vivo experiments have demonstrated that exosomes remain in undigested sponge material for two months,superior to their retention in fibrin glue,a commonly used biomaterial in clinical practice.Fibroin cryo-sponges were implanted subcutaneously in nude mice.The exosome-containing sponge group exhibited better neovascularization and tissue ingrowth effects,demonstrating the efficacy of this exosome-encapsulating strategy by realizing sustained release and maintaining exosome bioactivity.These silk fibroin cryo-sponges containing exosomes provide a new platform for future studies of exosome therapy. | Muyang Sun Qi Li Huilei Yu Jin Cheng Nier Wu Weili Shi Fengyuan Zhao Zhenxing Shao Qingyang Meng Haifeng Chen Xiaoqing Hu Yingfang Ao | 2022 | Bioactive Materials2022，7，2： | 4 |
| 3 | Spreading of Human Babesiosis in China:Current Epidemiological Status and Future Challenges显示文摘Human babesiosis is an infection caused by intraerythrocytic protozoa of the genus Babesia and is a worldwide emerging tick-borne disease(1).Babesia is mainly parasitized in erythrocytes of human or other vertebrates and transmitted by the bite of ticks,blood transfusion,or organ transplantation(1).Humans are generally susceptible to Babesia,especially those who are immunocompromised with conditions such as splenectomy or splenic dysfunction,as well as those of old age,with infirmity,or with other severe diseases. | Muxin Chen Qin Liu Jingbo Xue Shaohong Chen Dana Huang Yingfang Yu Yuchun Cai Yan Lu Peng Song Renli Zhang Lin Ai Jiaxu Chen | 2020 | China CDC weekly2020，2，33： | 3 |
| 4 | Extraterrestrial artificial photosynthetic materials for in-situ resource utilization显示文摘Aerospace milestones in human history,including returning to the moon and manned Martian missions,have been implemented in recent years.Space exploration has become one of the global common goals,and to ensure the survival and development of human beings in the extraterrestrial extreme environment has been becoming the basic ability and technology of manned space exploration.For the purpose of fulfilling the goal of extraterrestrial survival,researchers in Nanjing University and the China Academy of Space Technology proposed extraterrestrial artificial photosynthesis(EAP)technology.By simulating the natural photosynthesis of green plants on the Earth,EAP converts CO_(2)/H_(2)O into fuel and O_(2)in an in-situ,accelerated and controllable manner by using waste CO_(2)in the confined space of spacecraft,or abundant CO_(2)resources in extraterrestrial celestial environments,e.g.Mars.Thus,the material loading of manned spacecraft can be greatly reduced to support affordable and sustainable deep space exploration.In this paper,EAP technology is compared with existing methods of converting CO_(2)/H_(2)O into fuel and O_(2)in the aerospace field,especially the Sabatier method and Bosch reduction method.The research progress of possible EAP materials for in-situ utilization of extraterrestrial resources are also discussed in depth.Finally,this review lists the challenges that the EAP process may encounter,which need to be focused on for future implementation and application.We expect to deepen the understanding of artificial photosynthetic materials and technologies,and aim to strongly support the development of manned spaceflight. | Liuqing Yang Ce Zhang Xiwen Yu Yingfang Yao Zhaosheng Li Congping Wu Wei Yao Zhigang Zou | 2021 | National Science Review2021，8，8： | 3 |
| 5 | Surpassing electrocatalytic limit of earth-abundant Fe^(4+)embedded in N-doped graphene for(photo)electrocatalytic water oxidation显示文摘Developing highly active,cost-effective,and environmental friendly oxygen evolution reaction(OER)electrocatalysts facilitates various(photo)electrochemical processes.In this work,Fe3N nanoparticles encapsulated into N-doped graphene nanoshells(Fe_(3)N@NG)as OER electrocatalysts in alkaline media were reported.Both the experimental and theoretical comparison between Fe_(3) N@NG and Fe_(3)N/NG,specifically including in situ Mossbauer analyses,demonstrated that the NG nanoshells improved interfacial electron transfer process from Fe_(3)N to NG to form high-valence Fe^(4+)ions(Fe^(4+)@NG),thus modifying electronic properties of the outer NG shells and subsequently electron transfer from oxygen intermediate to NG nanoshells for OER catalytic process.Meanwhile,the NG nanoshells also protected Fe-based cores from forming OER inactive and insulated Fe_(2)O_(3),leading to high OER stability.As a result,the as-formed Fe^(4+)@NG shows one of the highest electrocatalytic efficiency among reported Fe-based OER electrocatalysts,which can as well highly improve the photoelectrochemical water oxidation when used as the cocatalysts for the Fe_(2)O_(3) nanoarray photoanode. | Wenjia Xue Feng Cheng Menglu Li Wenjian Hu Congping Wu Bing Wang Kuowei Liao ZhenTao Yu Yingfang Yao Wenjun Luo Zhigang Zou | 2021 | Journal of Energy Chemistry2021，30，3： | 2 |
| 6 | Medical Additive Manufacturing: From a Frontier Technology to the Research and Development of Products显示文摘1.Research and development(R&D)and the challenges of raw materials for medical additive manufacturing Raw materials for medical additive manufacturing have a wide range of commonalities that are also seen in many other fields,making them an important basis in the field of three-dimensional(3D)printing.Problems and challenges related to material types,powder properties,formability,viscoelasticity,and so forth also share common features.For example,many metal materials are used in the field of aviation,while metals,polymers,and inorganic materials are used in the field of biomedicine.The most widely used materials in biomedicine are biocompatible.Various homogeneous and non-homogeneous composites are also available for 3D printing,and impose an additional challenge in additive manufacturing;the use of heterogeneous composites in 3D printing is particularly challenging. | Guixing Qiu Wenjiang Ding Wei Tian Ling Qin Yu Zhao Lianmeng Zhang Jian Lu Daijie Chen Guangyi Yuan Chengtie Wu Bingheng Lu Ruxu Du Jimin Chen Mo Elbestawi Zhongwei Gu Dichen Li Wei Sun Yuanjin Zhao Jie He Dadi Jin Bin Liu Kai Zhang Jianmo Li Kam WLeong Dewei Zhao Dingjun Hao Yingfang Ao Xuliang Deng Huilin Yang ShaoKeh Hsu Yingqi Chen Long Li Jianping Fan Guohui Nie Yun Chen Hui Zeng Wei Chen Yuxiao Lai | 2020 | Engineering2020，6，11： | 1 |
| 7 | In situ resource utilization of lunar soil for highly efficient extraterrestrial fuel and oxygen supply显示文摘Building up a lunar settlement is the ultimate aim of lunar exploitation.Yet,limited fuel and oxygen supplies restrict human survival on the Moon.Herein,we demonstrate the in situ resource utilization of lunar soil for extraterrestrial fuel and oxygen production,which may power up our solely natural satellite and supply respiratory gas.Specifically,the lunar soil is loaded with Cu species and employed for electro catalytic CO_(2)conversion,demonstrating significant production of methane.In addition,the selected component in lunar soil(i.e.MgSiO_(3))loaded with Cu can reach a CH_(4)F aradaic efficiency of 72.05% with a CH_(4)production rate of 0.8 mL/min at 600 mA/cm^(2).Simultaneously,an O_(2)production rate of 2.3 mL/min can be achieved.Furthermore,we demonstrate that our developed process starting from catalyst preparation to electrocatalytic CO_(2)conversion is so accessible that it can be operated in an unmmaned manner via a robotic system.Such a highly efficient extraterrestrial fuel and oxygen production system is expected to push forward the development of mankind’s civilization toward an extraterrestrial settlement. | Yuan Zhong Jingxiang Low Qing Zhu Yawen Jiang Xiwen Yu Xinyu Wang Fei Zhang Weiwei Shang Ran Long Yingfang Yao Wei Yao Jun Jiang Yi Luo Weihua Wang Jinlong Yang Zhigang Zou Yujie Xiong | 2023 | National Science Review2023，10，2： | 1 |
| 8 | Analysis of Imaging Characteristics and Dynamic Changes of 3 Cases of Severe Novel Coronavirus Pneumonia in Qinghai Province显示文摘Objective:To analyze the characteristics,dynamic changes,and outcomes of the first imaging manifestations of 3 patients with severe COVID-19 in our hospital.Methods:Computed tomography(CT)findings of 3 patients with severe COVID-19 who tested positive by the nucleic acid test in our hospital were selected,mainly focusing on the morphology,distribution characteristics,and dynamic changes of the first CT findings.Results:3 patients with severe pneumonia were older,with one aged 80.The first chest CT examination for all 3 patients differed.Imaging showed a leafy distribution of consolidation,primarily affecting the lower lobes of both lungs and extending subpleurally.A grid-like pattern was observed,along with changes in the consolidation and air bronchogram.These changes had slower absorption,especially in patients with underlying diseases.Conclusion:CT manifestations of severe COVID-19 have specific characteristics and the analysis of their characteristics and dynamic changes provide valuable insights for clinical treatment. | Yingfang Yu Ruiyun Zhao Changde Li Fuqiang Ma Lingyun Guo Yang Li | 2024 | Journal of Clinical and Nursing Research2024，8，3： | 0 |
| 9 | Identification of microRNA and analysis of target genes in Panax ginseng显示文摘Objective: Ginsenosides, polysaccharides and phenols, the main active ingredients in Panax ginseng, are not different significantly in content between 3 and 5 years old of ginsengs called Yuan ginseng and more than ten years old ones called Shizhu ginseng. The responsible chemical compounds cannot fully explain difference in efficacy between them. According to reports in Lonicerae Japonicae Flos(Jinyinhua in Chinese) and Glycyrrhizae Radix et Rhizoma(Gancao in Chinese), microRNA may play a role in efficacy,so we identified microRNAs in P. ginseng at the different growth years and analyzed their target genes.Methods: Using high-throughput sequencing, the RNA-Seq, small RNA-Seq and degradome databases of P. ginseng were constructed. The differentially expressed microRNAs was identified by qRT-PCR.Results: A total of 63,875 unigenes and 24,154,579 small RNA clean reads were obtained from the roots of P. ginseng. From these small RNAs, 71 miRNA families were identified by bioinformatics target prediction software, including 34 conserved miRNAs, 37 non-conserved miRNA families, as well as 179 target genes of 17 known miRNAs. Through degradome sequencing and computation, we finally verified 13 targets of eight miRNAs involved in transcription, energy metabolism, biological stress and disease resistance, suggesting the significance of miRNAs in the development of P. ginseng. Consistently, major miRNA targets exhibited tissue specificity and complexity in expression patterns.Conclusion: Differential expression microRNAs were found in different growth years of ginsengs(Shizhu ginseng and Yuan ginseng), and the regulatory roles and functional annotations of miRNA targets in P. ginseng need further investigation. | Yingfang Wang Yanlin Chen Mengyuan Peng Chang Yang Zemin Yang Mengjuan Gong Yongqin Yin Yu Zeng | 2023 | Chinese Herbal Medicines2023，15，1： | 0 |
| 10 | MAP激酶级联通过调节ICE1蛋白质稳定性来调节冷应答显示文摘文章简介丝裂原活化蛋白激酶(MAPK)级联信号通路是将环境信号转化为细胞应答反应的重要信号模块。本研究发现,冷处理后MPK3、MPK4和MPK6可被迅速激活。 | Chunzhao Zhao Pengcheng Wang Tong Si Chuan-Chih Hsu Lu Wang Omar Zayed Zheping Yu Yingfang Zhu Juan Dong W.Andy Tao 朱健康 | 2018 | 科学新闻2018，0，4： | 0 |
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