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| 1 | Binding characteristics of perylene, phenanthrene and anthracene to different DOM fractions from lake water显示文摘Six hydrophobic and hydrophilic fractions were isolated using XAD-8 and XAD-4 resins, and were extensively characterized. Partition coefficients of perylene, phenanthrene and anthracene binding to the six fractions were determined by fluorescence quenching titration. The Kdoc values obtained for the polycyclic aromatic hydrocarbons (PAHs) binding to the hydrophobic fractions were larger than those to the hydrophilic fractions. Nonlinear Stern-Volmer plots were observed when binding phenanthrene and anthracene to some hydrophilic fraction samples, suggesting saturation of polar interaction binding sites. A significant correlation of logKdoc values with molecular weights and molar absorptivities at 280 nm was observed, while atomic ratio of C/H was found to be a poor indicator for aromaticity. Other structural descriptors such as paraffinic carbon and polarity influenced the DOM-fraction ability to bind PAHs. Different interaction mechanisms underlying binding of the different fractions to the PAHs were also discussed. | MEI Yi WU Fengchang WANG Liying BAI Yingchen LI Wen LIAO Haiqing | 2009 | Journal of Environmental Sciences2009，21，4： | 16 |
| 2 | Implantation of human umbilical cord mesenchymal stem cells for ischemic stroke: perspectives and challenges显示文摘Ischemic 击是经常导致严重地影响生活的质量的许多不利神经病学的复杂并发症的焦点的服的侮辱。击的流行在全世界正在增加,当当前的药理学治疗的功效仍然保持不清楚时。作为 neuroregenerative 治疗,间充质的干细胞(hUC-MSCs ) 显示出的人的脐的绳索的培植恢复的大可能性在击以后工作。这篇评论文章在 ischemic 击的处理提供 hUC-MSCs 培植的一个更改角色。与唯一抑制免疫力并且 immunoprivilege 性质, hUC-MSCs 被劝是 allogeneic 房间处理的一个重要候选人。不过,大多数处理仍然在主要舞台并且不临床上在当前的时间可行。几个不明确的问题例如文化条件, allograft 拒绝,和潜在的 tumorigenicity,是在这细胞的治疗的 choke 点。在 hUC-MSCs 培植能被用作习惯性地应用的临床的治疗以前,更现出症状之前的潜的研究和临床的研究被需要。 | Yingchen Li Guoheng Hu Qilai Chen | 2015 | Frontiers of Medicine2015，9，1： | 10 |
| 3 | Relationship between fluorescence characteristics and molecular weight distribution of natural dissolved organic matter in Lake Hongfeng and Lake Baihua,China显示文摘Dissolved organic matter (DOM) is one of the most interesting and difficult problems in recent years due to its important functions in the ecological and environmental system and the complexity of its chemical composition and structure. It is well ac- cepted that fluorescence characteristics and mo- lecular weight distribution are two important pa- rameters in the DOM characterization. However, the relationship between them is still unknown. In this study, fluorescence and molecular weight distribution of DOM in Lake Hongfeng, Lake Baihua and their rivers, and their relationship were investigated using the combination of fluorescence spectroscopy and high-performance size exclusion chromatography (HPSEC) with on-line UV absorbance and fluores- cence detectors. The results show that there were two obvious humic-like fluorescence peaks (Peaks A and B) in DOM from lake water. But there was an- other obvious protein-like fluorescence peak (Peak C) in DOM from river water. The humic-like fluorescence material consisted of DOM fraction with smaller mo- lecular weight, ranging from 1.0 to 3.0 kDa, while the protein-like fluorescence material mainly consisted of DOM fraction with MW larger than 2.0 kDa. The cal- culation of MW using HPSEC was related to the UV absorbance wavelength chosen. | YUE Lanxiu WU Fengchang LiU Congqiang LI Wen FU Pingqing BAI Yingchen WANG Liying YIN Zuoying Lü Zhicheng | 2006 | Chinese Science Bulletin2006，51，1： | 9 |
| 4 | Regulation of shear stress on rolling behaviors of HL-60 cells on P-selectin显示文摘Circulating leukocytes in trafficking to the inflammatory sites, will be first tether to, and then roll on the vascular surface. This event is mediated through specific interaction of P-selectin and P-selectin glycoprotein ligand-1(PSGL-1), and regulated by hemodynamics. Poor data were reported in understanding P-selectin-mediated rolling. With the flow chamber technique, we herein observed HL-60 cell rolling on P-selectin with or without 3% Ficoll at various wall shear stresses from 0.05 to 0.4 dyn/cm2. The results demonstrated that force rather than transport regulated the rolling, similar to rolling on L- and E-selectin. The rolling was accelerated quickly by an increasing force below the optimal shear threshold of 0.15 dyn/cm2 first and then followed by a slowly decelerating phase starting at the optimum, showing a catch-slip transition and serving as a mechanism for the rolling. The catch-slip transition was completely reflected to the tether lifetime and other rolling parameters, such as the mean and fractional stop time. The narrow catch bond regime stabilized the rolling quickly, through steeply increasing fractional stop time to a plateau of about 0.85. Data presented here suggest that the low shear stress threshold serves as a mechanism for most cell rolling events through P-selectin. | LING YingChen FANG Ying YANG XiaoFang LI QuHuan LIN QinYong WU JianHua | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，10： | 4 |
| 5 | Ultraviolet absorbance titration for the determination of conditional stability constants of Hg(Ⅱ) and dissolved organic matter显示文摘Strong interaction between natural dissolved organic matter (DOM) and Hg(Ⅱ) may influence the trans- port, conversion, toxicity and bio-validity of mercury in the environment. In this paper ultraviolet (UV) absorbance titration was employed for the first time for the determination of the conditional stability constants of Hg(Ⅱ) and (DOM). With increasing Hg(Ⅱ) concentrations, the UV absorbance of fulvic acid, humic acid, and DOM in river increases progressively. By linear and non-linear model fitting, the conditional stability constants (lgK) of Hg(Ⅱ) and DOM were worked out to be 3.54?4.93 and 3.64?4.85, respectively. The results are consistent with those acquired by the typical fluorescence quenching titration method, with the maximum relative error of lgK being 2.6% and the average relative error being 0.2%. The UV absorbance titration method has the advantages of rapid determination, simple performance, and it will probably become a new approach to studying interactions between DOM and trace metallic ions. | BAI Yingchen WU Fengchang WAN Guojing LIU Congqiang FU Pingqing LI Wen | 2008 | Chinese Journal Of Geochemistry2008，27，1： | 3 |
| 6 | Simulated photo-degradation of dissolved organic matter in lakes revealed by three-dimensional excitation-emission matrix with regional integration and parallel factor analysis显示文摘Simulated photo-degradation of fluorescent dissolved organic matter(FDOM) in Lake Baihua(BH) and Lake Hongfeng(HF) was investigated with three-dimensional excitationemission matrix(3 DEEM) fluorescence combined with the fluorescence regional integration(FRI),parallel factor(PARAFAC) analysis,and multi-order kinetic models.In the FRI analysis,fulvic-like and humic-like materials were the main constituents for both BH-FDOM and HF-FDOM.Four individual components were identified by use of PARAFAC analysis as humic-like components(C1),fulvic-like components(C2),protein-like components(C3) and unidentified components(C4).The maximum 3 DEEM fluorescence intensity of PARAFAC components C1-C3 decreased by about 60%,70% and 90%,respectively after photo-degradation.The multi-order kinetic model was acceptable to represent the photo-degradation of FDOM with correlation coefficient(Radj2)(0.963-0.998).The photo-degradation rate constants(kn) showed differences of three orders of magnitude,from 1.09 × 10-6 to 4.02 × 10-4 min-1,and half-life of multi-order model(T1/2n)ranged from 5.26 to 64.01 min.The decreased values of fluorescence index(FI) and biogenic index(BI),the fact that of percent fluorescence response parameter of Region I(PⅠ,n) showed the greatest change ratio,followed by percent fluorescence response parameter of Region II(PⅡ,n,while the largest decrease ratio was found for C3 components,and the lowest T1/2n was observed for C3,indicated preferential degradation of protein-like materials/components derived from biological sources during photodegradation.This research on the degradation of FDOM by 3 DEEM/FRI-PARAFAC would be beneficial to understanding the photo-degradation of FD OM in natural environments and accurately predicting the environmental behaviors of contaminants in the presence of FDOM. | Jin Zhang Fanhao Song Tingting Li Kefu Xie Huiying Yao Baoshan Xing Zhongyu Li Yingchen Bai | 2020 | Journal of Environmental Sciences2020，32，4： | 3 |
| 7 | Three decades of changes in water environment of a large freshwater Lake and its relationship with socio-economic indicators显示文摘Tai Lake(Ch: Taihu) has attracted international attention forcyanobacteria blooms.However, the drivers of cultural eutrophication, especially long-term socio-economic indicators have been little researched. The results of research demonstrate how socioeconomic development affected quality of water and how it has been improved by anthropogenic activities. This study described variability in indicators of water quality in Tai Lakeand investigated thedrivers. Significant relationships existed between concentrations of annual mean total nitrogen(TN), total phosphorous(TP), chemical oxygen demand(COD) and biological oxygen demand(BOD), and population, per capital gross domestic production(GDP) and sewage discharge(p < 0.05). However, mechanisms causing change varied among TN, TP, COD and BOD. Before 2000, the main contributors to increases in concentrations of TN were human population, GDP and volumes of domestic sewage discharges. After 2000, discharges of industrial sewage become the primary contributor. After 1998, the regressions of annual mean TN, TP and COD on per capital GDP, population and domestic sewage discharge were reversed compared to the former period. Since 1999, an apparent inverted U-shaped relationship between environmental pollution and economic development has developed, which indicated that actions taken by governments have markedly improved quality of water in Tai Lake. The statistical relationship between BOD and per capital GDP didn't conform to the Kuznet curve. The Ushaped Kuznet curve may offer hope for the future that with significant environmental investments a high GDP can be reached and maintained without degradation of the environment, especially through appropriate management of industrial sewage discharge. | Cuicui Li Weiying Feng Fanhao Song Zhongqi He Fengchang Wu Yuanrong Zhu John P.Giesy Yingchen Bai | 2019 | Journal of Environmental Sciences2019，31，3： | 3 |
| 8 | Extraction Technology of Total Flavonoids from Pueraria lobata (Willd.) Ohwi and Content Determination of Pueraria lobata (Willd.) Ohwi at Different Altitudes显示文摘[Objectives]To optimize the extraction technology of total flavonoids from Pueraria lobata( Willd.) Ohwi. [Methods]The ultraviolet-visible spectrophotometry was used to determine the content of total flavonoids in Pueraria lobata( Willd.) Ohwi. With the puerarin as index,the reflux extraction and single factor test were employed to investigate the effects of temperature,time,ethanol concentration and solid-liquid ratio on the content of total flavonoids in Pueraria lobata( Willd.) Ohwi,respectively. Under the optimal extraction technology,the content of total flavonoids in Pueraria lobata( Willd.) Ohwi at different altitudes was determined.[Results] The optimum extraction process was as follows: 70%ethanol; solid-liquid ratio of 1∶ 30; 1 h reflux extraction. Under these conditions,the extraction rate of flavonoids in Pueraria lobata( Willd.) Ohwi was 11. 48%,the total flavonoids content of different kudzu parts was in the order of roots > stems > leaves,and the total flavonoids content of the sample at about an altitude of 1000 m was significantly higher than at the altitudes of 1400 m and 1700 m.[Conclusions]It was suggested that the Pueraria lobata( Willd.) Ohwi should not be cultivated as medicinal plant in too high mountains,and the stems and leaves of Pueraria lobata( Willd.) Ohwi could be used as raw materials for extracting total flavonoids. | Chunlin LIU Yingchen LI Bochao LI Yuan LIU | 2017 | Medicinal Plant2017，8，2： | 2 |
| 9 | Aerodynamic inverse design optimization for turbine cascades based on control theory显示文摘Based on control theory,adjoint system for the general problem of turbomachinery aerodynamic optimization was studied and developed in the present paper by using the variation technique in the grid node coordinates combined with Jacobian Matrics of flow fluxes.Then the adjoint system for aerodynamic design optimization of turbine cascade governed by compressible Navier-Stokes equations was derived in detail.With the purpose of saving computation resources,the mathematic method presented in this paper avoids the coordinate system transforming in the traditional derivation process of the adjoint system and makes the adjoint system much more sententious.Given the general expression of objective functions consisting of both boundary integral and field integral,the adjoint equations and their boundary conditions were derived,and the final expression of the objective function gradient including only boundary integrals was formulated to reduce the CPU cost,especially for the complex 3D configurations.The adjoint system was solved numerically by using the finite volume method with an explicit 5-step Runge-Kutta scheme and Riemann approximate solution of Roe's scheme combined with multi-grid technique and local time step to accelerate the convergence procedure.Finally,based on the aerodynamic optimization theory in the present work,2D and 3D inviscid and viscous inverse design programs of axial turbomachinery cascade for both pressure distribution and isentropic Mach number distribution on the blade wall were developed,and several design optimization cases were performed successfully to demonstrate the ability and economy of the present optimization system. | FENG ZhenPing LI HaiTao SONG LiMing LI YingChen | 2013 | Science China(Technological Sciences)2013，56，2： | 2 |
| 10 | Organophosphate esters cause thyroid dysfunction via multiple signaling pathways in zebrafish brain显示文摘Organophosphate esters(OPEs)are widespread in various environmental media,and can disrupt thyroid endocrine signaling pathways.Mechanisms by which OPEs disrupt thyroid hormone(TH)signal transduction are not fully understood.Here,we present in vivo-in vitro-in silico evidence establishing OPEs as environmental THs competitively entering the brain to inhibit growth of zebrafish via multiple signaling pathways.OPEs can bind to transthyretin(TTR)and thyroxine-binding globulin,thereby affecting the transport of TH in the blood,and to the brain by TTR through the blood-brain barrier.When GH3 cells were exposed to OPEs,cell proliferation was significantly inhibited given that OPEs are competitive inhibitors of TH.Cresyl diphenyl phosphate was shown to be an effective antagonist of TH.Chronic exposure to OPEs significantly inhibited the growth of zebrafish by interfering with thyroperoxidase and thyroglobulin to inhibit TH synthesis.Based on comparisons of modulations of gene expression with the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases,signaling pathways related to thyroid endocrine functions,such as receptor-ligand binding and regulation of hormone levels,were identified as being affected by exposure to OPEs.Effects were also associated with the biosynthesis and metabolism of lipids,and neuroactive ligand-receptor interactions.These findings provide a comprehensive understanding of the mechanisms by which OPEs disrupt thyroid pathways in zebrafish. | Zhenfei Yan Chenglian Feng Xiaowei Jin Fangkun Wang Cong Liu Na Li Yu Qiao Yingchen Bai Fengchang Wu John P.Giesy | 2022 | Environmental Science and Ecotechnology2022，，4： | 2 |
| 11 | DICER1 mutations in a patient with an ovarian Sertoli-Leydig tumor, well-differentiated fetal adenocarcinoma of the lung, and familial multinodular goiter显示文摘 | Yingchen Wu Dongmei Chen Yangzhi Li Lijuan Bian Tingting Ma Meiqing Xie | 2014 | European Journal of Medical Genetics2014，，： | 1 |
| 12 | A preliminary study on pollution characteristics of surfactant substances in fine particles in the Beibu Gulf Region of China显示文摘The pollution characteristics of surfactant substances in fine particles(PM_(2.5))in spring were studied in the Beibu Gulf Region of China,68 samples of PM_(2.5)were collected at Weizhou Island in Beihai City from March 12 to April 17,2015.The Anionic Surfactant Substances(ASS)and Cationic Surfactant Substances(CSS)in the samples were analyzed using Byethyl Violet Spectrophotometry and Disulfide Blue Spectrophotometry,respectively.Combined with the data from backward trajectory simulation,the effects of air pollutants from remote transport on the pollution characteristics of surfactant substances in PM_(2.5)in the Beibu Gulf Region were analyzed and discussed.The results showed that the daily mean concentrations of ASS and CSS in spring in the Beibu Gulf Region were 165.20 pmol/m^(3)and 8.05 pmol/m^(3),and the variation ranges were 23.21–452.55 pmol/m^(3)and 0.65–31.31 pmol/m^(3),accounting for 1.82‰±1.65‰and 0.12‰±0.11‰of the mass concentration of PM_(2.5),respectively.These concentrations were lower than those in comparable regions around the world.There was no clear correlation between the concentrations of ASS and CSS in PM_(2.5)and the mass concentrations of PM_(2.5).Tourism and air transport had a positive contribution on the concentrations of ASS.The concentration of surfactant substances in PM_(2.5)was significantly impacted by wind speed and wind direction.Atmospheric temperature,air pressure and precipitation had little effect on the concentrations of surfactant substances.Surfactant substances in PM_(2.5)significantly impacted visibility.Results also showed that the main sources of surfactant substances were from the southern China and Southeast Asia. | Zhengzheng Zhang Hong Li Hongyan Liu Yingchen Bai Jinjuan Li Guorui Zhi Yanting Yu Wenjun Li Hao Zhang Fan Meng | 2021 | Journal of Environmental Sciences2021，33，4： | 0 |
| 13 | Population analysis reveals the roles of DNA methylation in tomato domestication and metabolic diversity显示文摘DNA methylation is an important epigenetic marker,yet its diversity and consequences in tomato breeding at the population level are largely unknown.We performed whole-genome bisulfite sequencing(WGBS),RNA sequencing,and metabolic profiling on a population comprising wild tomatoes,landraces,and cultivars.A total of 8,375 differentially methylated regions(DMRs)were identified,with methylation levels progressively decreasing from domestication to improvement.We found that over 20%of DMRs overlapped with selective sweeps.Moreover,more than 80%of DMRs in tomato were not significantly associated with single-nucleotide polymorphisms(SNPs),and DMRs had strong linkages with adjacent SNPs.We additionally profiled 339 metabolites from 364 diverse accessions and further performed a metabolic association study based on SNPs and DMRs.We detected 971 and 711 large-effect loci via SNP and DMR markers,respectively.Combined with multi-omics,we identified 13 candidate genes and updated the polyphenol biosynthetic pathway.Our results showed that DNA methylation variants could complement SNP profiling of metabolite diversity.Our study thus provides a DNA methylome map across diverse accessions and suggests that DNA methylation variation can be the genetic basis of metabolic diversity in plants. | Hao Guo Peng Cao Chao Wang Jun Lai Yuan Deng Chun Li Yingchen Hao Zeyong Wu Ridong Chen Qi Qiang Alisdair RFernie Jun Yang Shouchuang Wang | 2023 | Science China(Life Sciences)2023，66，8： | 0 |
| 14 | Elucidation of the melitidin biosynthesis pathway in pummelo显示文摘Specialized plant metabolism is a rich resource of compounds for drug discovery.The acylated flavonoid glycoside melitidin is being developed as an anti-cholesterol statin drug candidate,but its biosynthetic route in plants has not yet been fully characterized.Here,we describe the gene discovery and functional characterization of a new flavonoid gene cluster(UDP-glucuronosyltransferases(Cg UGTs),1,2rhamnosyltransferase(Cg1,2Rha T),acyltransferases(Cg ATs))that is responsible for melitidin biosynthesis in pummelo(Citrus grandis(L.)Osbeck).Population variation analysis indicated that the tailoring of acyltransferases,specific for bitter substrates,mainly determine the natural abundance of melitidin.Moreover,3-hydroxy-3-methylglutaryl-Co A reductase enzyme inhibition assays showed that the product from this metabolic gene cluster,melitidin,may be an effective anti-cholesterol statin drug candidate.Co-expression of these clustered genes in Nicotiana benthamiana resulted in the formation of melitidin,demonstrating the potential for metabolic engineering of melitidin in a heterologous plant system.This study establishes a biosynthetic pathway for melitidin,which provides genetic resources for the breeding and genetic improvement of pummelo aimed at fortifying the content of biologically active metabolites. | Shuangqian Shen Shouchuang Wang Chenkun Yang Chao Wang Qianqian Zhou Shen Zhou Ran Zhang Yufei Li Zixuan Wang Liupan Dai Wenjv Peng Yingchen Hao Hao Guo Guangping Cao Xianqing Liu Fan Yao Qiang Xu Alisdair RFernie Jie Luo | 2023 | Journal of Integrative Plant Biology2023，65，11： | 0 |
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