|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Hacking the optical diffraction limit: Review on recent developments of fluorescence nanoscopy显示文摘Subject to the diffraction limit, the resolution of conventional optical microscopy is constrained to about 200 and 500 nm in the lateral and axial planes, respectively. The advantage of optical microscopy in the life sciences over electronic microscopy, especially fluorescence microscopy, drives scientists to develop novel 'hacks' to reach nanoscale resolutions by optical means. In this review, three aspects of the techniques are discussed: (1) lateral super-resolution; (2) axial super-resolution; (3) super-resolution in three dimensions. The principles of how the methods achieve the cross-barrier resolution are discussed, and recent advances in current techniques are described. With these methods, the use of fluorescence microscopy is growing quickly toward a new era: fluorescence nanoscopy that will reveal 2 orders of magnitude more information on cellular structure and dynamics. | DING YiChen XI Peng REN QiuShi | 2011 | Chinese Science Bulletin2011，56，18： | 7 |
| 2 | Subvoxel light-sheet microscopy for high-resolution high-throughput volumetric imaging of large biomedical specimens显示文摘A key challenge when imaging whole biomedical specimens is how to quickly obtain massive cellular information over a large field of view(FOV).We report a subvoxel light-sheet microscopy(SLSM)method enabling high-throughput volumetric imaging of mesoscale specimens at cellular resolution.A nonaxial,continuous scanning strategy is developed to rapidly acquire a stack of large-FOV images with three-dimensional(3-D)nanoscale shifts encoded.Then,by adopting a subvoxel-resolving procedure,the SLSM method models these low-resolution,cross-correlated images in the spatial domain and can iteratively recover a 3-D image with improved resolution throughout the sample.This technique can surpass the optical limit of a conventional light-sheet microscope by more than three times,with high acquisition speeds of gigavoxels per minute.By fast reconstruction of 3-D cultured cells,intact organs,and live embryos,SLSM method presents a convenient way to circumvent the trade-off between mapping large-scale tissue(>100 mm3)and observing single cell(∼1-μm resolution).It also eliminates the need of complicated mechanical stitching or modulated illumination,using a simple light-sheet setup and fast graphics processing unit-based computation to achieve high-throughput,high-resolution 3-D microscopy,which could be tailored for a wide range of biomedical applications in pathology,histology,neuroscience,etc. | Peng Fei Jun Nie Juhyun Lee Yichen Ding Shuoran Li Hao Zhang Masaya Hagiwara Tingting Yu Tatiana Segura Chih-Ming Ho Dan Zhu Tzung KHsiaic | 2019 | Advanced Photonics2019，1，1： | 6 |
| 3 | Effect of heat-moisture treatment to raw paddy rice(Oryza sativa L.)On cooked rice properties显示文摘Heat moisture treatment is safe and non-chemical use method for modifying physicochemical properties and digestibility of starch.Slow starch digestion in rice product would provide numerous health benefi ts for the consumers.This study investigated the heat-moisture treatment to the raw paddy rice at 60 and 80ºC for 4 and 24 h,and their effects on the attributes of cooked rice.The fi rmness,adhesiveness,starch hydrolysis and morphological changes during in vitro digestion were examined.Results showed that heat-moisture treatment to raw paddy rice for 24 h signifi cantly reduced the moisture content and could increase the total starch content of the rice grain.The apparent amylose content ranged from 23.83%to 25.65%at the variation in the values between treated and untreated rice.Enhancement in the firmness and reduction in the starch hydrolysis was found in rice treated by longer heating time of 24 h.Meanwhile,the adhesiveness of cooked rice was decreased when increasing the heating temperature.The morphological observation showed that the treated rice exhibited a honey-comb-like structure during simulated gastric digestion,while the untreated rice displayed a non-uniform structure.Noteworthy,the porous structure observed in all digested rice grains during simulated small intestinal digestion could favor the starch hydrolysis.This study showed that the heat-moisture treatment to the raw paddy rice at certain conditions obviously influenced the attributes of cooked rice,especially on the starch hydrolysis,which would provide a fundamental knowledge to enable development and improvement of slow digestion rice products. | Sukanya Thuengtung Sunantha Ketnawa Yichen Ding Yukiharu Ogawa | 2021 | Journal of Future Foods2021，1，2： | 1 |
| 4 | Maternal genetic structure of a neolithic population of the Yangshao culture显示文摘The Yangshao culture is one of the most influential Neolithic cultures in East Asia.The populations associated with the Yangshao culture were widely distributed along the Yellow River basin and were suggested to play a profound role in the present-day East Asians.To date,the population of the Yangshao culture is largely investigated through archaeological studies. | Bo Miao Yichen Liu Wanfa Gu Qingli Wei Qian Wu Wenjun Wang Ming Zhang Manyu Ding Tianyi Wang Juncen Liu Feng Liu Peng Cao Qingyan Dai Ruowei Yang Xiaotian Feng Wanjing Ping Weihong Hou Haibing Yuan Qiaomei Fu | 2021 | Journal of Genetics and Genomics2021，48，8： | 0 |
| 5 | TNFR2 is a potent prognostic biomarker for post-transplant lung metastasis in patients with hepatocellular carcinoma显示文摘Objective:Lung metastasis is a common and fatal complication of liver transplantation for hepatocellular carcinoma(HCC).The precise prediction of post-transplant lung metastasis in the early phase is of great value.Methods:The mRNA profiles of primary and paired lung metastatic lesions were analyzed to determine key signaling pathways.We enrolled 241 HCC patients who underwent liver transplantation from three centers.Tissue microarrays were used to evaluate the prognostic capacity of tumor necrosis factor(TNF),tumor necrosis factor receptor 1(TNFR1),and TNFR2,particularly for post-transplant lung metastasis.Results:Comparison of primary and lung metastatic lesions revealed that the TNF-dependent signaling pathway was related to lung metastasis of HCC.The expression of TNF was degraded in comparison to that in para-tumor tissues(P<0.001).The expression of key receptors in the TNF-dependent signaling pathway,TNFR1 and TNFR2,was higher in HCC tissues than in para-tumor tissues(P<0.001).TNF and TNFR1 showed no relationship with patients’outcomes,whereas elevated TNFR2 in tumor tissue was significantly associated with worse overall survival(OS)and increased recurrence risk(5-year OS rate:31.9%vs.62.5%,P<0.001).Notably,elevated TNFR2 levels were also associated with an increased risk of post-transplant lung metastasis(hazard ratio:1.146;P<0.001).Cox regression analysis revealed that TNFR2,Hangzhou criteria,age,and hepatitis B surface antigen were independent risk factors for post-transplant lung metastasis,and a novel nomogram was established accordingly.The nomogram achieved excellent prognostic efficiency(area under time-dependent receiver operating characteristic=0.755,concordance-index=0.779)and was superior to conventional models,such as the Milan criteria.Conclusions:TNFR2 is a potent prognostic biomarker for predicting post-transplant lung metastasis in patients with HCC.A nomogram incorporating TNFR2 deserves to be a helpful prognostic tool in liver transplantation for HCC. | Huigang Li Zuyuan Lin Jianyong Zhuo Modan Yang Wei Shen Zhihang Hu Yichen Ding Hao Chen Chiyu He Xinyu Yang Siyi Dong Xuyong Wei Beicheng Sun Shusen Zheng Ren Lang Di Lu Xiao Xu | 2023 | Chinese Journal of Cancer Research2023，35，1： | 0 |
| 6 | An updated constraint on the local stratigraphy at the Chang'E-4 landing site显示文摘The Chang’E-4 mission has been exploring the lunar farside.Two scientific targets of the rover onboard are(1)resolving the possible mineralogy related to the South Pole-Aitken basin and(2)understanding the subsurface processes at the lunar farside.Publications to date that are based on the reflectance spectra and radar data obtained by the rover have shown a persistent inconsistency about the local stratigraphy.To explain both the abnormal surface topography at the landing site and the unexpected radargram observed by the rover,the Alder crater has been frequently reported to be older than the mare basalts at that landing site.However,this argument is not supported by earlier geological mapping nor recent crater statistics.Resolving this controversy is critical for a full understanding of the geological history of the landing area and for correct interpretations of the scientific data returned.Employing detailed crater statistics,rigorous statistical analyses,and an updated crater chronology function,this study is determined to resolve the relative ages of the Alder crater,Finsen crater,and the mare basalts on the floor of Von Kármán.Our results reveal that while background secondaries and local resurfacing have widely occurred in the study area,affecting age determinations,the statistics are significant enough to conclude that the Alder crater is the oldest among the three targets.This independent constraint is consistent with both the crosscutting relationships of different terrains in this area and global stratigraphic mapping.Our results exclude Alder as a possible contributor of the post-mare deposits at the landing site,appealing for a more systematic stratigraphy study to resolve the provenances of these deposits. | YiRen Chang ZhiYong Xiao YiChen Wang ChunYu Ding Jun Cui YuZhen Cai | 2021 | Earth and Planetary Physics2021，5，1： | 0 |
| 7 | Design strategies for rechargeable aqueous metal-ion batteries显示文摘Rechargeable aqueous metal-ion batteries(AMBs)have attracted extensive scientific and commercial interest due to their potential for cost-effective,highly safe,and scalable stationary energy storage.However,their limited output voltage,inadequate energy density,and poor reversibility of ambiguous electrode reactions in aqueous electrolytes strongly limit their practical viability.This review aims to elucidate the challenges of existing AMBs from the material design to whole device applications.We summarize the emerging electrochemistry,fundamental properties,and key issues in interfacial behaviors of various classes of prevailing AMBs,including aqueous alkali metal-ion batteries and multivalent-ion batteries,and present an appraisal of recent advances for addressing the performance deficiency.Specifically,the progress of zinc-ion batteries is highlighted to provide a ubiquitous guideline for their commercialization in the grid-scale energy storage.Finally,we figure out the dominating general challenges for achieving high-performance AMBs,laying out a perspective for future breakthroughs. | Yang Li Xin Zhao Yifu Gao Yichen Ding Zhichun Si Liubing Dong Dong Zhou Feiyu Kang | 2024 | Science China Chemistry2024，67，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员