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| 1 | Induction of mitochondrion-mediated apoptosis of CHO cells by tripchloro lide显示文摘Tripchlorolide (TC) is a potent antitumor reagent purified from a Chinese herb Tripterygium Wilfordii Hook. f.. However, its cellular effects and mechanism of action are unknown. We showed here that TC induced apoptosis of Chinese Hamster Ovary (CHO) cells in time- and dose-dependent manners. TC resulted in the degradation of Bcl-2, the translocation of Bax from the cytosol to mitochondria, and the release of cytochrome c from mitochondria. Stable overexpression of human Bcl-2 could reduce the apoptosis of TCtreated cells by blocking the translocation of Bax and the release of cytochrome c. These results indicate that TC induces apoptosis of CHO cell by activating the mitochondrion-mediated apoptotic pathway involving the proteins of Bcl-2 family and cytochrome c. | YANREN LEIXIONG JIARUIWU | 2003 | Cell Research2003，13，4： | 11 |
| 2 | The C-Terminal Portion of the Nucleocapsid Protein Demonstrates SARS-CoV Antigenicity显示文摘In order to develop clinical diagnostic tools for rapid detection of SARS-CoV (severe acute respiratory syndrome-associated coronavirus) and to identify candidate proteins for vaccine development, the C-terminal portion of the nucleocapsid (NC)gene was amplified using RT-PCR from the SARS-CoV genome, cloned into a yeast expression vector (pEGH), and expressed as a glutathione S-transferase (GST) and Hisx6 double-tagged fusion protein under the control of an inducible promoter.Western analysis on the purified protein confirmed the expression and purification of the NC fusion proteins from yeast. To determine its antigenicity, the fusion protein was challenged with serum samples from SARS patients and normal controls.The NC fusion protein demonstrated high antigenicity with high specificity, and therefore, it should have great potential in designing clinical diagnostic tools and provide useful information for vaccine development. | GuozhenLiu ShaohuiHu YongwuHu PengChen JianningYin JieWen JingqiangWang LiangLin JinxiuLiu BoYou YeYin ShutingLi HaoWang YanRen JiaJi XiaoqianZhao YongqiaoSun XiaoweiZhang JianqiuFang JianWang SiqiLiu JunYu HengZhu HuanmingYang | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 4 |
| 3 | Text memorization and imitation: The practices of successful Chinese learners of English显示文摘 | Yanren Ding | 2007 | System2007，，2： | 2 |
| 4 | Differential activation of intra-S-phase checkpoint in response to tripchlorolide and its effects on DNA replication显示文摘DNA replication is tightly regulated during the S phase of the cell cycle, and the activation of the intra-S-phase checkpoint due to DNA damage usually results in arrest of DNA synthesis. However, the molecular details about the correlation between the checkpoint and regulation of DNA replication are still unclear. To investigate the connections between DNA replication and DNA damage checkpoint, a DNA-damage reagent, tripchlorolide, was applied to CHO (Chinese ovary hamster) cells at early- or middle-stages of the S phase. The early-S-phase treatment with TC significantly delayed the progression of the S phase and caused the phosphorylation of the Chk1 checkpoint protein, whereas the middle-S-phase treatment only slightly slowed down the progression of the S phase. Furthermore, the analysis of DNA replication patterns revealed that replication pattern Ⅱ was greatly prolonged in the cells treated with the drug during the early-S phase, whereas the late-replication patterns of these cells were hardly detected, suggesting that the activation of the intra-S-phase checkpoint inhibits the late-origin firing of DNA replication. We conclude that cells at different stages of the S phase are differentially sensitive to the DNA-damage reagent, and the activation of the intra-Sphase checkpoint blocks the DNA replication progression in the late stage of S phase. | YanREN JiaRuiWU | 2004 | Cell Research2004，14，3： | 2 |
| 5 | Uniform Attractors for Three-Dimensional Navier-Stokes Equations with NonlinearDamping 显示文摘 | SONG Xueli HOU Yanren | 2015 | J Math Anal Appl2015，422，1： | 1 |
| 6 | The Pullback Asymptotic Behavior of the Solutions for 2D Nonautonomous G-Navier-Stokes Equations显示文摘The pullback asymptotic behavior of the solutions for 2D Nonautonomous G-Navier-Stokes equations is studied,and the existence of its L^(2)-pullback attractors on some bounded domains with Dirichlet boundary conditions is investigated by using the measure of noncompactness.Then the estimation of the fractal dimensions for the 2D G-Navier-Stokes equations is given. | Jinping Jiang Yanren Hou Xiaoxia Wang | 2012 | Advances in Applied Mathematics and Mechanics2012，4，2： | 1 |
| 7 | FULL DISCRETE TWO-LEVEL CORRECTION SCHEME FOR NAVIER-STOKES EQUATIONS显示文摘在这篇论文,为不稳定的海军司烧方程的一个完整的分离二水平的计划基于一条时间依赖者设计途径被建议。在新设计和它切开的相关空间的意义,非线性仅仅在粗糙的水平候补选手空间被对待在当一个线性方程不得不在好水平候补选手空间被解决在这时候得到最后的近似时,每次由确切解决标准 Galerkin 方程走步。因此,它是为标准 Galerkin 近似的一个二水平的基于的修正计划。为这个计划的稳定性和错误估计在纸被调查。 | Yanren Hou Liquan Mei | 2008 | Journal of Computational Mathematics2008，26，2： | 1 |
| 8 | Application of proprotein convertase subtilisin/kexin type 9 inhibitor, evolocumab, in patients with severe hypertriglyceridemia显示文摘To the Editor:The proprotein convertase subtilisin/kexin type 9(PCSK9)-inhibitor,evolocumab,can bind with PCSK9 in plasma and reduce the degradation of the PCSK9-low density lipoprotein(LDL)receptor complex,thereby reducing LDL levels.[1]In clinical practice,PCSK9 inhibitors can reduce low-density lipoprotein cholesterol(LDL-c)significantly and reduce triglycerides(TG)by about 8%to 17%.[2]We used PCSK9 inhibitors to lower blood lipids in three patients with severe hypertriglyceridemia(HTG)and performed whole-exome sequencing(WES)to analyze the genes that may contribute to hyperlipidemia in detail.We have obtained patients'consent forms.We found that PCSK9 inhibitors significantly reduced blood lipid levels,regardless of the presence of HTG mutations.These results provide new evidence for the use of PCSK9 inhibitors in the treatment of patients with severe HTG. | Yanren Peng Danxia Guo Sijie Jiang Hua Zheng | 2022 | Chinese Medical Journal2022，，6： | 1 |
| 9 | The uniform attractor for the 2D non-autonomous Navier-Stokes flow in some unbounded domain显示文摘 | Yanren Hou Kaitai Li | 2004 | Nonlinear Analysis2004，58，： | 1 |
| 10 | Attractors for the three-dimensional incompressible Navier Stokes equations with damping显示文摘 | Song Xueli Hou Yanren | 2011 | Discrete and Continuous Dynamical Systems2011，31，1： | 1 |
| 11 | A finite element variational multiscale method for incompressible flows based on two local gauss integrations显示文摘 | Haibiao Zheng Yanren Hou Feng Shi Lina Song | 2009 | Journal of Computational Physics2009，，16： | 1 |
| 12 | Sample Collection,DNA Extraction,and Library Construction Protocols of the Human Microbiome Studies in the International Human Phenome Project显示文摘The human microbiome plays a crucial role in human health.In the past decade,advances in high-throughput sequencing technologies and analytical software have significantly improved our knowledge of the human microbiome.However,most studies concerning the human microbiome did not provide repeatable protocols to guide the sample collection,handling,and processing procedures,which impedes obtaining valid and timely microbial taxonomic and functional results.This protocol provides detailed operation methods of human microbial sample collection,DNA extraction,and library construction for both the amplicon sequencing-based measurements of the microbial samples from the human nasal cavity,oral cavity,and skin,as well as the shotgun metagenomic sequencing-based measurements of the human stool samples among adult par-ticipants.This study intends to develop practical procedure standards to improve the reproducibility of microbiota profiling of human samples. | Yetong Wang Ruyi Zhang Yanni Pu Danqi Wang Yanren Wang Xuemei Wu Yujie Pan Chen Luo Guoping Zhao Zhexue Quan Yan Zheng | 2023 | Phenomics2023，3，3： | 0 |
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