5篇
您的检索式：作者名="Yanjun Pang"
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| 1 | Detection of the Pandemic H1N1/2009 Influenza A Virus by a Highly Sensitive Quantitative Real-time Reverse-transcription Polymerase Chain Reaction Assay显示文摘A quantitative real time reverse-transcription polymerase chain reaction (qRT-PCR) assay with specific primers recommended by the World Health Organization (WHO) has been widely used successfully for detection and monitoring of the pandemic H1N1/2009 influenza A virus. In this study, we report the design and characterization of a novel set of primers to be used in a qRT-PCR assay for detecting the pandemic H1N1/2009 virus. The newly designed primers target three regions that are highly conserved among the hemagglutinin (HA) genes of the pandemic H1N1/2009 viruses and are different from those targeted by the WHO-recommended primers. The qRT-PCR assays with the newly designed primers are highly specific, and as specific as the WHO-recommended primers for detecting pandemic H1N1/2009 viruses and other influenza viruses including influenza B viruses and influenza A viruses of human, swine, and raccoon dog origin. Furthermore, the qRT-PCR assays with the newly designed primers appeared to be at least 10-fold more sensitive than those with the WHO-recommended primers as the detection limits of the assays with our primers and the WHO-recommended primers were 2.5 and 25 copies of target RNA per reaction, respectively. When tested with 83 clinical samples, 32 were detected to be positive using the qRT-PCR assays with our designed primers, while only 25 were positive by the assays with the WHO-recommended primers. These results suggest that the qRT-PCR system with the newly designed primers represent a highly sensitive assay for diagnosis of the pandemic H1N1/2009 virus infection. | Zhu Yang Guoliang Mao Yujun Yuan-Chuan Chen Chengjing Liu Jun Luo Xihan Li Ke Zen Yanjun Pang Jianguo Wu Fenyong Liu | 2013 | Virologica Sinica2013，28，1： | 2 |
| 2 | An Effective Calculating Method of Reducing Attributes Classified into Some Grades显示文摘 | Kaidi Liu Yanjun Pang Yinao Wang | 2003 | Advancesin Systens Science and Applications2003，3，1： | 1 |
| 3 | Effects of the polysaccharide from Pholiota nameko on human cytokine network in serum显示文摘 | Haiping Li Xiaojuan Liu Yang Li Yanjun Hua Dexian Zhi Guangchang Pang | 2011 | International Journal of Biological Macromolecules2011，，1： | 1 |
| 4 | Expression of the 78kD glucose-regulated protein is induced by endoplasmic reticulum stress in the development of hepatopulmonary syndrome显示文摘 | Huiying Zhang Minli Lv Jiantao Jia Zhongfu Zhao Lili Zhang Lina Lai Yanjun Wu Baohong Li Chen Li Yan Liu Xujiong Li Xiaoxia Tian Hui Pang Jianhong Guo Limin Wang Yimin Fan Cuiying Zhang Jingquan Ji Dewu Han Cheng Ji | 2013 | Gene2013，，： | 1 |
| 5 | Light-guided tumor diagnosis and therapeutics:From nanoclusters to polyoxometalates显示文摘Malignant tumors,with the characteristics of easy metastasis and recurrence,are a serious threat to health of mankind.It is urgent to develop promising clinical cancer targeted agents with combination of rapid diagnosis and efficient therapies.Compared with the conventional photosensitizing agents,the recent advances of nanoagents based on transition metal-oxide clusters possess unique structural and electronic properties,greatly improving cancer survival rate,meanwhile,keeping high contrast imaging.This review provides a brief introduction of metal-oxide clusters,including both nanoclusters to molecular clusters,specifically polyoxometalates(POMs).Subsequently,biocompatibility of metal-oxide clusters is emphasized from aspects of endocytosis,macropinocytosis,and phagocytosis.Through the classification of late and early transition metals oxide clusters,recent outcomes of light-guided nanoagents are represented with their intriguing chemical and optical properties in their diagnosing and photochemotherapy performance.It shed light on the summary of next generation multifunctional cancer targeting agents'developments as well as outlook of materials selection trends and research direction in the future. | Xiaofeng Fan Wei Pang Hao Feng Ruiyi Zhang Wentao Zhu Qiushi Wang Jun Miao Yiwen Li Yanjun Liu Xiaoqian Xu | 2022 | Chinese Chemical Letters2022，33，6： | 0 |
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