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| 1 | Efficient genome editing in plants using a CRISPR/Cas system显示文摘 | Zhengyan Feng Botao Zhang Wona Ding Xiaodong Liu Dong-Lei Yang Pengliang Wei Fengqiu Cao Shihua Zhu Feng Zhang Yanfei Mao Jian-Kang Zhu | 2013 | Cell Research2013，23，10： | 211 |
| 2 | Application of the CRISPR-Cas System for Efficient Genome Engineering in Plants显示文摘 | Mao, Yanfei Zhang, Hui Xu, Nanfei Zhang, Botao Gou, Feng Zhu, Jian-Kang | 2013 | Molecular Plant2013，6，6： | 132 |
| 3 | Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System显示文摘 | Mugui Wang Yanfei Mao Yuming Lu Xiaoping Tao Jian-kang Zhu | 2017 | Molecular Plant2017，10，7： | 56 |
| 4 | Gene Targeting by Homology-Directed Repair in Rice Using a Geminivirus-Based CRISPR/Cas9 System显示文摘 | Mugui Wang Yuming Lu Jose Ramon Botella Yanfei Mao Kai Hua Jian-kang Zhu | 2017 | Molecular Plant2017，10，7： | 34 |
| 5 | Effects of organic acids on the leaching process of ion-adsorption type rare earth ore显示文摘To examine the activation of organic acids on the leaching process of ion-adsorption type rare earth ore(IRE-ore), the leaching behavior of rare earth(RE) and zeta potential of IRE-ore were investigated in the absence and presence of carboxylic acids. The results show that all the tested organic acids(acetic acid,malonic acid, citric acid, tartaric acid, succinic acid, and malic acid) can promote RE extraction. At relatively high concentrations of organic acids, the activation efficiency of organic acids on RE extraction is generally consistent with their complexation ability; whereas at their low concentrations, the change of zeta potential on the IRE-ore surface with organic acid concentration and p H has a close association with RE extraction, which indicates that organic acids can impact the surface electrical property of IREore via their adsorption/desorption, and thereby increase/decrease the affinity of RE ions to IRE-ore.Therefore the influence of organic acids on the IRE-ore surface electrical property also plays an important role in RE extraction in addition to their complexation with RE ions. | Li Wang Chunfa Liao Youming Yang Haibo Xu Yanfei Xiao Chunhua Yan | 2017 | Journal of Rare Earths2017，35，12： | 23 |
| 6 | Hybrid Material of Graphene and Poly(3,4-ethyldioxythiophene)with High Conductivity,Flexibility,and Transparency显示文摘A novel hybrid material prepared from graphene and poly(3,4-ethyldioxythiophene)(PEDOT)shows excellent transparency,electrical conductivity,and good flexibility,together with high thermal stability and is easily processed in both water and organic solvents.Conductivities of the order of 0.2 S/cm and light transmittance of greater than 80%in the 4001800 nm wavelength range were observed for fi lms with thickness of tens of nm.Practical applications in a variety of optoelectronic devices are thus expected for this transparent and flexible conducting graphene-based hybrid material。 | Yanfei Xu Yan Wang Jiajie Liang Yi Huang Yanfeng Ma Xiangjian Wan Yongsheng Chen | 2009 | Nano Research2009，2，4： | 23 |
| 7 | Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems显示文摘We developed simplified single transcriptional unit(SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its cr RNA array are co-expressed from a single Pol Ⅱ promoter, without any additional processing machinery. Our SSTU systems are easy to construct and effective in mediating multiplex genome editing. | Mugui Wang Yanfei Mao Yuming Lu Zhidan Wang Xiaoping Tao Jian-Kang Zhu | 2018 | Journal of Integrative Plant Biology2018，60，8： | 21 |
| 8 | The material and biological characteristics of osteoinductive calcium phosphate ceramics显示文摘The discovery of osteoinductivity of calcium phosphate(Ca-P)ceramics has set an enduring paradigm of conferring biological regenerative activity to materials with carefully designed structural characteristics.The unique phase composition and porous structural features of osteoinductive Ca-P ceramics allow it to interact with signaling molecules and extracellular matrices in the host system,creating a local environment conducive to new bone formation.Mounting evidence now indicate that the osteoinductive activity of Ca-P ceramics is linked to their physicochemical and three-dimensional structural properties.Inspired by this conceptual breakthrough,many laboratories have shown that other materials can be also enticed to join the rank of tissue-inducing biomaterials,and besides the bones,other tissues such as cartilage,nerves and blood vessels were also regenerated with the assistance of biomaterials.Here,we give a brief historical recount about the discovery of the osteoinductivity of Ca-P ceramics,summarize the underlying material factors and biological characteristics,and discuss the mechanism of osteoinduction concerning protein adsorption,and the interaction with different types of cells,and the involvement of the vascular and immune systems. | Zhurong Tang Xiangfeng Li Yanfei Tan Hongsong Fan Xingdong Zhang | 2018 | Regenerative Biomaterials2018，5，1： | 20 |
| 9 | Recanalization of extracranial internal carotid artery occlusion A 12-year retrospective study显示文摘This study aimed to summarize therapy experience of carotid endarterectomy, carotid endarterec-tomy combined with Fogarty catheter embolectomy, and hybrid surgery for the treatment of extrac-ranial internal carotid artery occlusion. The study included 65 patients with extracranial internal ca-rotid artery occlusion who underwent carotid endarterectomy, carotid endarterectomy combined with Fogarty catheter embolectomy, or hybrid surgery in the Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, China between January 2006 and December 2012. Prior to surgery, all patients underwent perfusion CT or xenon CT to evaluate the occlusion. The procedure for each patient was chosen according to digital subtraction angiography data. The carotid artery was successfully recanalized in 46 of 51 patients who underwent carotid endarterectomy, 9 of 10 patients who underwent carotid endarterectomy combined with Fogarty catheter embolectomy, and 3 of 4 patients who underwent hybrid surgery. In patients with symptomatic carotid artery occlusion, the carotid artery can be recanalized by choosing a treatment procedure based on imaging ex-amination findings. | Liqun Jiao Gang Song Yang Hua Yan Ma Yanfei Chen Yabing Wang Feng Ling | 2013 | Neural Regeneration Research2013，8，23： | 19 |
| 10 | A cellular automata evacuation model considering friction and repulsion显示文摘There exist interactions among pedestrians and between pedestrian and environment in evacuation. These interactions include attraction, repulsion and friction that play key roles in human evacuation behaviors, speed and efficiency. Most former evacuation models focus on the attraction force, while repulsion and friction are not well modeled. As a kind of multi-particle self-driven model, the social force model introduced in recent years can represent those three forces but with low simulation efficiency because it is a continuous model with complex rules. Discrete models such as the cellular automata model and the lattice gas model have simple rules and high simulation efficiency, but are not quite suitable for interactions’ simulation. In this paper, a new cellular automata model based on traditional models is introduced in which repulsion and friction are modeled quantitatively. It is indicated that the model can simulate some basic behaviors, e.g. arching and the “faster-is-slower” phenomenon, in evacuation as multi-particle self-driven models, but with high efficiency as the normal cellular automata model and the lattice gas model. | SONG Weiguo YU Yanfei FAN Weicheng Zhang Heping | 2005 | Science China(Technological Sciences)2005，48，4： | 19 |
| 11 | A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana显示文摘The newly developed CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats)/Cas(CRISPR-associated) system has emerged as an efficient tool for genome-editing, providing an alternative to classical mutagenesis and transgenic methods to study gene function and improve crop traits. CRISPR/Cas facilitates targeted gene editing through RNA-guided DNA cleavage followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. Here we describe a detailed procedure for our previously developed and highly efficient CRISPR/Cas9 method that allows the generation of heritable-targeted gene mutations andcorrections in Arabidopsis. This protocol describes the strategies and steps for the selection of targets, design of single-guide RNA(sg RNA), vector construction and analysis of transgenic lines. We also offer a method to target two loci simultaneously using vectors containing two different sg RNAs. The principles described in this protocol can be applied to other plant species to generate stably inherited DNA modifications. | Wenshan Liu Xiaohong Zhu Mingguang Lei Qingyou Xia Jose Ramon Botella Jian-Kang Zhu Yanfei Mao | 2015 | Science Bulletin2015，60，15： | 18 |
| 12 | Gene editing in plants:progress and challenges显示文摘The clustered regularly interspaced short palindromic repeat(CRISPR)-associated protein 9(Cas9)genome editing system is a powerful tool for targeted gene modifications in a wide range of species,including plants.Over the last few years,this system has revolutionized the way scientists perform genetic studies and crop breeding,due to its simplicity,flexibility,consistency and high efficiency.Considerable progress has been made in optimizing CRISPR/Cas9 systems in plants,particularly for targeted gene mutagenesis.However,there are still a number of important challenges ahead,including methods for the efficient delivery of CRISPR and other editing tools to most plants,and more effective strategies for sequence knock-ins and replacements.We provide our viewpoint on the goals,potential concerns and future challenges for the development and application of plant genome editing tools. | Yanfei Mao Jose Ramon Botella Yaoguang Liu Jian-Kang Zhu | 2019 | National Science Review2019，6，3： | 17 |
| 13 | Current Situation of Heavy Metal Pollution in Farmland Soil and Phytoremediation Application显示文摘Phytoremediation technology is a newly-developed way of soil heavy metal pollution repair with high efficiency and good ecological comprehensive benefit. This paper briefly introduces the soil heavy metal pollution status at home and abroad,and focuses on the analysis of harm,sources and current situation of soil heavy metal pollution at home and abroad as well as mechanism and application of phytoremediation.Finally it discusses the key problems in phytoremediation technology that need to resolve in the future. | Yanfei HUANG Guifen CHEN Liumei XIONG Yuyi HUANG | 2016 | Asian Agricultural Research2016，8，1： | 15 |
| 14 | Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting显示文摘Generating B cell-deficient mutant is the first step to produce human antibody repertoires in large animal models. In this study, we applied the clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas) system to target the JHregion of the pig Ig M heavy chain gene which is crucial for B cell development and differentiation. Transfection of Ig M-targeting Cas9 plasmid in primary porcine fetal fibroblasts(PFFs) enabled inducing gene knock out(KO) in up to 53.3% of colonies analyzed, a quarter of which harbored biallelic modification, which was much higher than that of the traditional homologous recombination(HR). With the aid of somatic cell nuclear transfer(SCNT) technology, three piglets with the biallelic Ig M heavy chain gene mutation were produced. The piglets showed no antibody-producing B cells which indicated that the biallelic mutation of the Ig M heavy chain gene effectively knocked out the function of the Ig M and resulted in a B cell-deficient phenotype. Our study suggests that the CRISPR/Cas9 system combined with SCNT technology is an efficient genome-editing approach in pigs. | Fengjiao Chen Ying Wang Yilin Yuan Wei Zhang Zijian Ren Yong Jin Xiaorui Liu Qiang Xiong Qin Chen Manling Zhang Xiaokang Li Lihua Zhao Ze Li Zhaoqiang Wu Yanfei Zhang Feifei Hu Juan Huang Rongfeng Li Yifan Dai | 2015 | Journal of Genetics and Genomics2015，42，8： | 15 |
| 15 | Non-ammonia enrichment of rare earth by magnesium oxide from rare earth leaching liquor in magnesium salt system显示文摘In order to solve the problem of ammonia-nitrogen pollution in the enrichment process of the ionadsorption type rare earth ore,the technology of non-ammonia precipitation with magnesium oxide precipitant was carried out.It is determined that the rare earth precipitation efficiency is 99.6%and the purity of rare earth concentrates is only 85.89 wt%under the optimum precipitation conditions.And the contents of MgO,SO3 and Al2O3 in the rare earth concentrates are 5.12 wt%,6.77 wt%and 1.78 wt%,respectively.Furthermore,the thermo-decomposition process of precipitates was investigated by TGDSC,XRD and FI-IR.The thermal decomposition process consists of two stages:the dehydration of rare earth hydroxide and alkaline rare earth sulfate within 900℃and the thermal decomposition of RE2O2SO4 at 900-1300℃.Therefore,a high-temperature calcinations method for removing SO3 from precipitates is proposed.When the precipitates are calcined at 1300℃for 2 h,the rare earth concentrates with a purity of 92.03 wt%can be acquired.Moreover,the content of SO3 in the concentrate is only 0.46 wt%.In the MgO precipitation and high-temperature calcinations process,the raw material cost is low and the quality of rare earth concentrates is acceptable.It could have great significance for nonammonia enrichment of rare earth from the rare earth leaching liquor,and finally solve the problem of ammonia nitrogen in the extraction process of the ion-adsorption type rare earth ore within magnesium salt system. | Li Huang Guohua Gao Ran Wu Qian Zhang Fuguo Lai Yanfei Xiao | 2019 | Journal of Rare Earths2019，37，8： | 14 |
| 16 | A unifying approach in simulating the shot peening process using a 3D random representative volume finite element model显示文摘Using a modified 3D random representative volume(RV)finite element model,the effects of model dimensions(impact region and interval between impact and representative regions),model shapes(rectangular,square,and circular),and peening-induced thermal softening on resultant critical quantities(residual stress,Almen intensity,coverage,and arc height)after shot peening are systematically examined.A new quantity,i.e.,the interval between impact and representative regions,is introduced and its optimal value is first determined to eliminate any boundary effect on shot peening results.Then,model dimensions are respectively assessed for all model shapes to reflect the actual shot peening process,based on which shape-independent critical shot peening quantities are obtained.Further,it is found that thermal softening of the target material due to shot peening leads to variances of the surface residual stress and arc height,demonstrating the necessity of considering the thermal effect in a constitutive material model of shot peeing.Our study clarifies some of the finite element modeling aspects and lays the ground for accurate modeling of the SP process. | Dianyin HU Ye GAO Fanchao MENG Jun SONG Yanfei WANG Mengxi REN Rongqiao WANG | 2017 | Chinese Journal of Aeronautics2017，30，4： | 13 |
| 17 | Review of Hydrogen Embrittlement in Metals: Hydrogen Diffusion, Hydrogen Characterization, Hydrogen Embrittlement Mechanism and Prevention显示文摘Hydrogen dissolved in metals as a result of internal and external hydrogen can affect the mechanical properties of the metals, principally through the interactions between hydrogen and material defects. Multiple phenomena such as hydrogen dissolution, hydrogen diffusion, hydrogen redistribution and hydrogen interactions with vacancies, dislocations, grain boundaries and other phase interfaces are involved in this process. Consequently, several hydrogen embrittlement(HE) mechanisms have been successively proposed to explain the HE phenomena, with the hydrogen-enhanced decohesion mechanism, hydrogenenhanced localized plasticity mechanism and hydrogen-enhanced strain-induced vacancies being some of the most important. Additionally, to reduce the risk of HE for engineering structural materials in service, surface treatments and microstructural optimization of the alloys have been suggested. In this review, we report on the progress of the studies on HE in metals, with a particular focus on steels. It focuses on four aspects:(1) hydrogen diffusion behavior;(2) hydrogen characterization methods;(3) HE mechanisms;and(4) the prevention of HE. The strengths and weaknesses of the current HE mechanisms and HE prevention methods are discussed, and specific research directions for further investigation of fundamental HE mechanisms and methods for preventing HE failure are identified. | Xinfeng Li Xianfeng Ma Jin Zhang Eiji Akiyama Yanfei Wang Xiaolong Song | 2020 | Acta Metallurgica Sinica(English Letters)2020，33，6： | 13 |
| 18 | Effects of Rare Earth elements on microstructure evolution and mechanical properties of 718H pre-hardened mold steel显示文摘718 H Pre-ha rdened mold steels with diffe rent Rare Earth(RE) contents were prepared to investigate the influence of RE on microstructure evolution and mechanical properties through a series of experiments and theoretical analysis.The results indicated that the toal oxygen(T.O) content decreased from 15 ppm to 6 ppm with 0.022 wt% RE addition,which is attributed to the active chemical properties of RE elements.For test steels,RE additions of 0.012 wt% and 0.022 wt% were significantly effective for refining inclusions by eliminating 11.5% large-sized inclusions with diameter exceeding 10 μm compared with that of ORE steel.RE addition contributed to modify irregular MnS and Al2 O3 inclusions into ellipsoidal RE-inclusions(RE2 O,RES,RE2 O2 S and REAlO3).The purification of molten steel and the modification of inclusions by RE treatment have significant effects on improvement of the fatigue crack growth tests(FCG) inhibition ability and impact energy as well as the isotropy.However,excessive addition of RE elements(0.07 wt%)seriously reduced the impact energy,ultimate tensile strength and FCG inhibition ability due to rapidly increase of the volume fraction of large-sized inclusions.In addition to the inclusions formed by RE treatment,trace solid solution RE atoms improve the stability of undercooled austenite,resulting in the transformation region of bainite and perlite of 0.07 RE steel shifting to the bottom right and prolonging the incubation period compared with that of ORE steel. | Hanghang Liu Paixian Fu Hongwei Liu Yanfei Cao Chen Sun Ningyu Du Dianzhong Li | 2020 | Journal of Materials Science & Technology2020，47，15： | 11 |
| 19 | Regional Distribution and Sustainable Development Strategy of Mineral Resources in China显示文摘This paper summarizes the distribution and production layout of the major mineral resources in China,including coal,iron ore,copper and bauxite,from a national perspective.It also identifies the incompatibility between the mineral resources distribution and regional economic development.Significant issues with China's mineral resource industry cause challenges for the sustainable development of both the mining industry and the national socio-economy.The sustainability of regional mineral resources and the environmental pollution by mining in the western China were also analyzed.Results show that the distribution of China's mineral resources is misaligned with its regional layout of economic development.China's mineral resources have been over-exploited,and the mineral resources production in the eastern China is unsustainable.The continuously expanding production of mineral resources in the western China has heavily endangered the ecological environment.We propose strategies to boost the sustainable development of mineral resources,including measures to accelerate economic development and enhance the sustainability of domestic mineral resources.We also offer suggestions for scientifically planning the mineral resource prospecting and exploitation and regional economic layout,as well as for proactively undertaking industry transfer in the eastern China and raising the environmental benchmark requirements for the mineral industry in the central and western China. | LI Chaofeng WANG Anjian CHEN Xiaojin CHEN Qishen ZHANG Yanfei LI Ying | 2013 | Chinese Geographical Science2013，23，4： | 11 |
| 20 | A Domestication-Associated Gene GmPRR3b Regulates the Circadian Clock and Flowering Time in Soybean显示文摘Improved soybean cultivars have been adapted to grow at a wide range of latitudes,enabling expansion of cultivation worldwide.However,the genetic basis of this broad adaptation is still not clear.Here,we report the identification of GmPRR3b as a major flowering time regulatory gene that has been selected during domestication and genetic improvement for geographic expansion.Through a genome-wide association study of a diverse soybean landrace panel consisting of 279 accessions,we identified 16 candidate quantitative loci associated with flowering time and maturity time.The strongest signal resides in the known flowering gene E2,verifying the effectiveness of our approach.We detected strong signals associated with both flowering and maturity time in a genomic region containing GmPRR3b.Haplotype analysis revealed that GmPRR3bH6 is the major form of GmPRR3b that has been utilized during recent breeding of modern cultivars.mRNA profiling analysis showed that GmPRR3bH6 displays rhythmic and photoperiod-dependent expression and is preferentially induced under long-day conditions.Overexpression of GmPRR3bH6 increased main stem node number and yield,while knockout of GmPRR3bH6 using CRISPR/Cas9 technology delayed growth and the floral transition.GmPRR3bH6 appears to act as a transcriptional repressor of multiple predicted circadian clock genes,including GmCCAIa,which directly upregulates J/GmELF3a to modulate flowering time.The causal SNP(Chr12:5520945)likely endows GmPRR3bH6 a moderate but appropriate level of activity,leading to early flowering and vigorous growth traits preferentially selected during broad adaptation of landraces and improvement of cultivars. | Cong Li Ying-hui Li Yanfei Li Hongfeng Lu Huilong Hong Yu Tian Hongyu Li Tao Zhao Xiaowei Zhou Jun Liu Xinan Zhou Scott A.Jackson Bin Liu Li-juan Qiu | 2020 | Molecular Plant2020，13，5： | 11 |
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