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| 1 | Activation of protein kinase A alters subnuclear distribution pattern of human steroidogenic factor 1 in living cells显示文摘Background The aim of this study was to identify the subnuclear distribution pattern of human orphan nuclear receptor steroidogenic factor 1 (SF-1) in living cells with and without the activation of protein kinase A (PKA) signal pathway, and thus try to explain the unknown mechanism by which PKA potentiates SF-1 transactivation. Methods Full-length cDNAs of wild type and a naturally occurring mutant (G35E) human SF-1 were cloned and fused with green fluorescent protein (GFP). Subcellular distribution pattern of human SF-1 in living cells, whose PKA signaling was either activated or not, was studied by laser confocal microscopy after the validity of the gene sequence was confirmed.Results The transactivation ability of the GFP-SF-1 chimeric protein was highly conserved. Wild type human SF-1 diffused homogeneously within the nuclei of cells when PKA was not active, and converged to clear foci when PKA was activated. Mutant SF-1 diffused within the nuclei even in the presence of PKA activation, surprisingly aggregating as fluorescent dots inside the nucleoli, a phenomenon not altered by PKA.Conclusions Activation of PKA causes wild type, but not mutant SF-1 to alter its subnuclear distribution pattern to a transactivationally active form (foci formation). This finding may throw new light on the mechanism by which PKA activates the orphan nuclear receptor. | 刘伟 范吴强 Toshihiko Yanase Masayuki Saitoh 吴茵 | 2004 | Chinese Medical Journal2004，，7： | 3 |
| 2 | Induction of ischemic tolerance in rat liver via reduced nicotinamide adenine dinucleotide phosphate oxidase in Kupffer cells显示文摘AIM: To elucidate the mechanisms of hepatocyte preconditioning by H2O2 to better understand the pathophysiology of ischemic preconditioning. METHODS: The in vitro effect of H2O2 pretreatment was investigated in rat isolated hepatocytes subjected to anoxia/reoxygenation. Cell viability was assessed with propidium iodide fluorometry. In other experiments, rat livers were excised and subjected to warm ischemia/ reperfusion in an isolated perfused liver system to determine leakage of liver enzymes. Preconditioning was performed by H2O2 perfusion, or by stopping the perfusion for 10 min followed by 10 min of reperfusion. To inhibit Kupffer cell function or reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, gadolinium chloride was injected prior to liver excision, or diphenyleneiodonium, an inhibitor of NADPH oxidase, was added to the perfusate, respectively. Histological detection of oxygen radical formation in Kupffer cells was performed by perfusion with nitro blue tetrazolium. RESULTS: Anoxia/reoxygenation decreased hepatocyte viability compared to the controls. Pretreatment with H2O2 did not improve such hepatocyte injury. In liver perfusion experiments, however, H2O2 preconditioning reduced warm ischemia/reperfusion injury, which wasreversed by inhibition of Kupffer cell function or NADPH oxidase. Histological examination revealed that H2O2 preconditioning induced oxygen radical formation in Kupffer cells. NADPH oxidase inhibition also reversed hepatoprotection by ischemic preconditioning. CONCLUSION: H2O2 preconditioning protects hepato- cytes against warm ischemia/reperfusion injury via NADPH oxidase in Kupffer cells, and not directly. NADPH oxidase also mediates hepatoprotection by ischemic preconditioning. | Kazuaki Tejima Masahiro Arai Hitoshi Ikeda Tomoaki Tomiya Mikio Yanase Yukiko Inoue Takako Nishikawa Naoko Watanabe Natsuko Ohtomo Masao Omata Kenji Fujiwara | 2007 | World Journal of Gastroenterology2007，13，38： | 2 |
| 3 | SJWD14101600000482显示文摘 | Naoyoshi Nagata Ryota Niikura Tomonori Aoki Takuro Shimbo Yoshihiro Kishida Katsunori Sekine Shohei Tanaka Kazuhiro Watanabe Toshiyuki Sakurai Chizu Yokoi Junichi Akiyama Mikio Yanase Masashi Mizokami Naomi Uemura | 2014 | J Gastroenterol Hepatol2014，，10： | 2 |
| 4 | Lower GI bleeding risk of nonsteroidal anti-inflammatory drugs and antiplatelet drug use alone and the effect of combined therapy显示文摘 | Naoyoshi Nagata Ryota Niikura Tomonori Aoki Takuro Shimbo Yoshihiro Kishida Katsunori Sekine Shohei Tanaka Hidetaka Okubo Kazuhiro Watanabe Toshiyuki Sakurai Chizu Yokoi Junichi Akiyama Mikio Yanase Masashi Mizokami Naomi Uemura | 2014 | Gastrointestinal Endoscopy2014，，： | 2 |
| 5 | Absorption of Cu2+ or Hg2+ ion on resin prepared by crosslinking metalcomplexed chitosans显示文摘 | OHGA K KURAUCHI Y YANASE H | 1987 | Bull Chem Soc Jpn1987，60，1： | 2 |
| 6 | Increase in colonic diverticulosis and diverticular hemorrhage in an aging society: lessons from a 9-year colonoscopic study of 28,192 patients in Japan显示文摘 | Naoyoshi Nagata Ryota Niikura Tomonori Aoki Takuro Shimbo Toshiyuki Itoh Yoshimasa Goda Ryuichiro Suda Hideaki Yano Junichi Akiyama Mikio Yanase Masashi Mizokami Naomi Uemura | 2014 | International Journal of Colorectal Disease2014，，3： | 2 |
| 7 | 17a-hydroxylase/17,20-1yase deficiency: from clinical investigation to molecular definition显示文摘 | YANASE T SIMPSON E R WATERMAN M R | 1991 | Endocr Rew1991，12，： | 1 |
| 8 | Androgen - insensitivity syndrome as a possible coactivator disease显示文摘 | Adachi M TR Tomura A Imasaki K Kato S Goto K Yanase T Ikuyama S Nawata H | 2000 | N Engl J Med2000，343，： | 1 |
| 9 | Mutation of cytochrome P-45017 alpha gene (CYP17) in a Japanese patient previously reported as having glucocorticoid responsive hyperaldosteronism:with a review of Japanese patients with mutations of CYP17显示文摘 | Miura K Yasuda K Yanase T | 1996 | J Clin Endocrinol Metab1996，81，： | 1 |
| 10 | Cyclic glucans produced by the intramolccular transglycosylation activity of potato D-enzyme on amylopcctin显示文摘 | TAKAHA T YANASE M TAKATA H | 1998 | Biochemical and Biophysical Research Communications1998，247，： | 1 |
| 11 | Inhibition of DNA topoisomerases Ⅰ and Ⅱ, and growth inhibition of human cancer cell lines by a marine microalgal polysaccharide 显示文摘 | Umemura K Yanase K Suzuki M | 2003 | Bioehem Pharmacol2003，66，3： | 1 |
| 12 | Insulin sensitizer,troglitazone,directly inhibits aromatase activity in human ovarian granulosa cells 显示文摘 | Mu YM Yanase T Nishi Y | 2000 | Biochem Biophys Res Commun2000，271，： | 1 |
| 13 | Genetic reassortment between Sathuperi and Shamonda viruses of the genus Orthobun- yavirus in nature: implications for their genetic relationship to Schmallenberg virus 显示文摘 | Yanase T Kato T Aizawa M | 2012 | Arch Virol2012，157，8： | 1 |
| 14 | Mild hypothermia-a revived countermeasure against ischemic neuronal damages显示文摘 | Kataoka K Yanase H | 1998 | Neurosci Res1998，32，2： | 1 |
| 15 | Simultaneous detection of bovine arboviruses using single-tube multiplex reverse transcription-polymerase chain reaction 显示文摘 | Ohashi S Yoshida K Yanase T | 2004 | Journal of Virological Methods2004，120，： | 1 |
| 16 | Purification andcharacterization of new aldehyde reductases from Sporobolomycessalmonicolor AKU4429显示文摘 | KITA K NAKASE K YANASE H | 1999 | J Mol Catal B:Enzymatic1999，6，： | 1 |
| 17 | Tissue inhibitor of metalloproteinases-1 attenuates spontaneous liver fibrosis resolution in the transgenic mouse显示文摘 | Hitoshi Yoshiji Shigeki Kuriyama Junichi Yoshii Yasuhide Ikenaka Ryuichi Noguchi Toshiya Nakatani Hirohisa Tsujinoue Koji Yanase Tadashi Namisaki Hiroo Imazu Hiroshi Fukui | 2002 | Hepatology2002，，4： | 1 |
| 18 | Induction of endometrial cycles and ovulation in a woman with combined 17α-hydroxylase/ 17,20 lyase deficiency due to compound heteroxygous mutations on the P450 17αgene显示文摘 | Matsuzaki S Yanase T Murakami T | 2000 | Fertil Steril2000，7，6： | 1 |
| 19 | Change in microRNAs associat- ed with neuronal adaptive responses in the nucleus accumbens under neuropathic pain显示文摘 | Imai S Saeki M Yanase M | 2011 | J Neurosei2011，31，15： | 1 |
| 20 | Effects of ionizing radiation on locomotory behavior and mechanosensation in Caenorhabditis elegans显示文摘 | Suzuki M Sakashita T Yanase S | | 0，，02： | 1 |
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