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| 1 | QTG-Seq Accelerates QTL Fine Mapping through QTL Partitioning and Whole-Genome Sequencing of Bulked Segregant Samples显示文摘Deciphering the genetic mechanisms underlying agronomic traits is of great importance for crop improvement. Most of these traits are controlled by multiple quantitative trait loci (QTLs), and identifying the underlying genes by conventional QTL fine-mapping is time-consuming and labor-intensive. Here, we devised a new method, named quantitative trait gene sequencing (QTG-seq), to accelerate QTL fine-mapping. QTGseq combines QTL partitioning to convert a quantitative trait into a near-qualitative trait, sequencing of bulked segregant pools from a large segregating population, and the use of a robust new algorithm for identifying candidate genes. Using QTG-seq, we fine-mapped a plant-height QTL in maize (Zea mays L.), qPH7, to a 300-kb genomic interval and verified that a gene encoding an NF-YC transcription factor was the functional gene. Functional analysis suggested that qPH7-encoding protein might influence plant height by interacting with a CO-like protein and an AP2 domain-containing protein. Selection footprint ana卜 ysis indicated that qPH7 was subject to strong selection during maize improvement. In summary, QTG-seq provides an efficient method for QTL fine-mapping in the era of “big data'. | Hongwei Zhang Xi Wang Qingchun Pan Pei Li Yunjun Liu Xiaoduo Lu Wanshun Zhong Minqi Li Linqian Han Juan Li Pingxi Wang Dongdong Li Yan Liu Qing Li Fang Yang Yuan-Ming Zhang Guoying Wang Lin Li | 2019 | Molecular Plant2019，12，3： | 13 |
| 2 | The U6 Biogenesis-Like I Plays an Important Role in Maize Kernel and Seedling Development by Affecting the 3' End Processing of U6 snRNA显示文摘 | Jiankun Li Junjie Fu Yan Chen Kaijian Fan Cheng He Zhiqiang Zhang Li Li Yunjun Liu Jun Zheng Dongtao Ren Guoying Wang | 2017 | Molecular Plant2017，10，3： | 5 |
| 3 | Ferrimagnetic m PEG-b-PHEP copolymer micelles loaded with iron oxide nanocubes and emodin for enhanced magnetic hyperthermia–chemotherapy显示文摘As a non-invasive therapeutic method without penetration-depth limitation,magnetic hyperthermia therapy(MHT)under alternating magnetic field(AMF)is a clinically promising thermal therapy.However,the poor heating conversion efficiency and lack of stimulus–response obstruct the clinical application of magnetofluid-mediated MHT.Here,we develop a ferrimagnetic polyethylene glycol-poly(2-hexoxy-2-oxo-1,3,2-dioxaphospholane)(m PEG-b-PHEP)copolymer micelle loaded with hydrophobic iron oxide nanocubes and emodin(denoted as EMM).Besides an enhanced magnetic resonance(MR)contrast ability(r2=271 m M^-1 s^-1)due to the high magnetization,the specific absorption rate(2518 W/g at 35 k A/m)and intrinsic loss power(6.5 n Hm2/kg)of EMM are dozens of times higher than the clinically available iron oxide nanoagents(Feridex and Resovist),indicating the high heating conversion efficiency.Furthermore,this composite micelle with a flowable core exhibits a rapid response to magnetic hyperthermia,leading to an AMF-activated supersensitive drug release.With the high magnetic response,thermal sensitivity and magnetic targeting,this supersensitive ferrimagnetic nanocomposite realizes an above 70%tumor cell killing effect at an extremely low dosage(10μg Fe/m L),and the tumors on mice are completely eliminated after the combined MHT–chemotherapy. | Yonghong Song Dongdong Li Yang Lu Kun Jiang Yi Yang Yunjun Xu Liang Dong Xu Yan Daishun Ling Xianzhu Yang Shu-Hong Yu | 2020 | National Science Review2020，7，4： | 4 |
| 4 | Methods of Studying Petroleum Systems in Residual Basins-A Case Study of Mesozoic and Palaeozoic MarineBasins in Southern China显示文摘 | ZHAO Zongju, ZHU Yan, YANG Shufeng, WANG Genghai,XU Yunjun, FENG Jialiang and DENG Hongying Department of Geoscience, Zhejiang University, Hangzhou 310027, Zhejiang Hangzhou Institute of Petroleum Geology, Hangzhou 310023, Zhejiang Zhou Jian Fei Zhenbi | 2002 | Acta Geologica Sinica(English Edition)2002，76，2： | 4 |
| 5 | Biodiesel synthesis combining pre-esterification with alkali catalyzed process from rapeseed oil deodorizer distillate显示文摘 | Liu Yun Wang Ling Yan Yunjun | 2009 | Fuel Processing Technology2009，90，78： | 1 |
| 6 | Cloning, expression and characterization of a novel thermal stable and short-chain alcohol tolerant lipase from Burkholderia cepacia strain G63显示文摘 | Jiangke Yang Daoyi Guo Yunjun Yan | 2007 | B, Enzymatic2007，，3： | 1 |
| 7 | Anti-tumor effect of々-elemene in murine hepatocellular carcinoma cell line H22 dependson the level of c-Met downregulation显示文摘 | YAN Qina GUO Yunjun WEI Wei | 2012 | Biomedicine and PreventiveNutrition2012，2，2： | 1 |
| 8 | Production of biodieselcatalyzed by immobilized Pseudomonas cepacia lipase from Sapium sebiferum oil in micro-aqueous phase显示文摘 | Qin Li Yunjun Yan | 2010 | Applied Energy2010，87，： | 1 |
| 9 | Optimization of Lipase-Catalyzed Transesterification of Lard for Biodiesel Production Using Response Surface Methodology显示文摘 | Ying Huang Hai Zheng Yunjun Yan | 2010 | Applied biochemistry and biote- chnology2010，160，2： | 1 |
| 10 | Cloning, expression and characterization of a novel thermal stable and short-chain alcohol tolerant lipase from Burkholderia cepacia strain G63 显示文摘 | Yang Jiangke Guo Daoyi Yan Yunjun | 2007 | Journal of Molecular Catalysis B:Enzymatic2007，45，34： | 1 |
| 11 | Physicochemical properties of stillingia oil: feasibility for biodiesel production by enzyme transesterification 显示文摘 | Liu Yun Xin Hongling Yan Yunjun | 2009 | Industrial Crops and Products2009，30，3： | 1 |
| 12 | The pentatricopeptide repeat protein EMP601 functions in maize seed development by affecting RNA editing of mitochondrial transcript ccmC显示文摘Although several pentatricopeptide repeat(PPR) proteins are involved in post-transcriptional processing of mitochondrial RNA, it is unclear which specific protein is involved in the RNA editing of ccmC in maize(Zea mays). Here we report the identification of the maize empty pericarp 601(emp601) mutant and the map-based cloning of the Emp601 gene, which encodes an E2-type PPR protein that is targeted to mitochondria. A single-nucleotide deletion in the emp601 mutant caused a frameshift and introduced a premature stop codon into the predicted EMP601. This mutation was associated with reduced accumulation of mitochondrial complex Ⅲ as well as with inhibition of growth and differentiation of basal endosperm transfer layer cells, leading to final degeneration of the embryo and endosperm. We determine that loss of EMP601 function prevents the C-to-U RNA editing of the mitochondrial transcript ccmC at position 358.EMP601 binds to the ccmC transcript and directly interacts with Multiple organellar RNA editing factor 8and may be a component of the plant mitochondrial editosome. We conclude that EMP601 functions in RNA editing of mitochondrial ccmC transcripts and influences mitochondrial function and seed development. | Rongrong Chen Qianhan Wei Yan Liu Jiankun Li Xuemei Du Yan Chen Jianhua Wang Yunjun Liu | 2023 | The Crop Journal2023，11，5： | 0 |
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