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| 1 | Role of CD56-expressing immature biliary epithelial cells in biliary atresia显示文摘AIM: To analyze the clinical and pathological parameters and expression of the neural cell adhesion molecule(CD56) in patients with biliary atresia(BA).METHODS: Established clinical laboratory markers of hepatic function, including enzyme activity, protein synthesis, and bilirubin metabolism, were evaluated in patients with BA and compared with those in patients with choledochal cysts and neonatal hepatitis. Pathological changes in tissue morphology and fibrosis were examined by histological and tissue collagen staining. Immunohistochemical staining for the biliary epithelial cell markers CD56 and CK19 together with the Notch signaling related molecules Notch1 and Notch2 was performed in the context of alterations in the structure of intrahepatic biliary ducts.RESULTS: Differences in some clinical laboratoryparameters among the three diseases examined were observed, but they did not correlate with the pathological classification of fibrosis in BA. Immunohistochemical staining showed the presence of CD56-positive immature bile ducts in most patients(74.5%) with BA but not in patients with choledochal cysts or neonatal hepatitis. The number of CD56-expressing cells correlated with disease severity, with more positive cells present in the later stages of liver damage(81.8% vs 18.2%). Furthermore, bile plugs were mainly found in CD56-positive immature biliary ducts. Notch signaling was a key regulatory pathway in biliary duct formation and played a role in tissue fibrosis. Notch1 was co-expressed in CD56-positive cells, whereas Notch2 was found exclusively in blood vessels in the portal area of patients with BA. CONCLUSION: The maturation of biliary epithelial cells and the expression of Notch may play a role in the pathogenesis of BA. | Rui-Zhong Zhang Jia-Kang Yu Jiao Peng Feng-Hua Wang Hai-Ying Liu Vincent CH Lui John M Nicholls Paul KH Tam Jonathan R Lamb Yan Chen Hui-Min Xia | 2016 | World Journal of Gastroenterology2016，22，8： | 8 |
| 2 | Micronuclei in Nasal Mucosa, Oral Mucosa and Lymphocytes in Students Exposed to Formaldehyde Vapor in Anatomy Class显示文摘The frequency of micronuclei (MN) in cells of the nasal mucosa, oral mucosa and in lym-phocytes was evaluated for 25 students in anatomy classes exposed to formaldehyde (FA) overan 8-week period. Each student served as his or her own control. The time-weighted averageconcentration (TWA) of fOrmaldehyde in anatomical laboratories and in students’ dormitorieswas 0. 508 ± 0. 299 mg/m3 and 0. 012 ± 0. 0025mg/m3, respectively. A higher frequency ofmicronuclei was observed in nasal and oral exfoliative cells after formaldehyde exposure (3. 85± 1. 48 vs 1 .20± 0. 676 and 0. 857 ± 0. 558 vs 0. 568 ± 0. 317, paired-t test: P< 0. 001and P < 0. 01, respectively). No significant increase in the frequency of lymphocyte micronu-clei was found after formaldehyde exposure (P >0. 05 ). The present study shows that nasalmucosa cel1s exposed through respiration are the chief target of FA-induced genotoxic | YING CHEN -JlANG YAN WEN-SHENG ZHAO MEI-YING YE XIAO-LEI XIE HONG YIN SHU-YI ZHU XIAN-SHU(Department of Environmental Health, n Dgurtment of Anatomy,Tongji the ical University, Wuhan, China)(Department of Public Health, Wengzhou theical Chllege, Ch | 1997 | Biomedical and Environmental Sciences1997，10，4： | 7 |
| 3 | Effect of Qinling granule in treatment of 102 patients with chronic hepatitis B显示文摘EfectofQinlinggranuleintreatmentof102patientswithchronichepatitisBHUANGZhengMing1,YANGXinBo1,CAOWenBin1,ZHOUYiJun2andLULi... | HUANG Zheng Ming 1, YANG Xin Bo 1, CAO Wen Bin 1, ZHOU Yi Jun 2 and LU Li Yan 2 1Department of Pharmacology, Chinese PLA Beijing Medical School, Beijing 100071, China 2Chinese PLA 184 Hospital, Yingtan 350000, Jiangxi Province, Ch | 1998 | World Journal of Gastroenterology1998，4，1： | 7 |
| 4 | Downregulation of Hes1 expression in experimental biliary atresia and its effects on bile duct structure显示文摘AIM To analyze the expression and function of the notchsignaling target gene Hes1 in a rhesus rotavirusinduced mouse biliary atresia model. METHODS The morphologies of biliary epithelial cells in biliary atresia patients and in a mouse model were examined by immunohistochemical staining. Then, the differential expression of Notch signaling pathway-related molecules was investigated. Further, the effects of the si RNAmediated inhibition of Hes1 expression were examined using a biliary epithelial cell 3 D culture system.RESULTS Both immature(Ep CAM+) and mature(CK19+) biliary epithelial cells were detected in the livers of biliary atresia patients without a ductile structure and in the mouse model with a distorted bile duct structure. The hepatic expression of transcripts for most Notch signaling molecules were significantly reduced on day 7 but recovered to normal levels by day 14, except for the target molecule Hes1, which still exhibited lower m RNA and protein levels. Expression of the Hes1 transcriptional co-regulator, RBP-Jκ was also reduced. A 3 D gel culture system promoted the maturation of immature biliary epithelial cells, with increased expression of CK19+ cells and the formation of a duct-like structure. The administration of Hes1 si RNA blocked this process. As a result, the cells remained in an immature state, and no duct-like structure was observed.CONCLUSION Our data indicated that Hes1 might contribute to the maturation and the cellular structure organization of biliary epithelial cells, which provides new insight into understanding the pathology of biliary atresia. | Rui-Zhong Zhang Xin-Hao Zeng Ze-Feng Lin Ming-Fu Yan-Lu Tong Vincent CH Lui Paul KH Tam Jonathan R Lamb Hui-Min Xia Yan Chen | 2018 | World Journal of Gastroenterology2018，24，29： | 5 |
| 5 | Split liver transplantation: a reliable approach to expand donor pool显示文摘Orthotopic liver transplantation as a successful treatment of end-stage liver disease is hampered by a persistent lack of cadaveric organs. Split liver transplantation, which was first successfully performed by Medical School of Hannover in 1988, has become a mature surgical technique to expand the donor pool. Between 1993 and 1999, split liver transplantation activities have increased in Europe from 1. 2% to 10. 4% in all performed liver transplantations. Current data have strongly supported that the survival rate of patients after split liver transplantation is not significantly different from that of patients after whole-size orthotopic liver transplantation. The most important step of donor graft selection is surgeon’ s observation judged by the experience of individual transplant center. The paper aims to provide the guideline of donor selection, hepatic graft splitting, and recipient management as well. DATA SOURCES: Medical School of Hannover has accumulated plentiful experience of split liver transplantation for more than 10 cases ever since 1998. Besides that, we also reviewed a variety of literatures from other famous European and American centers specialized in this field for many years. RESULTS:According to our experience combined with the view points of others, the donor should meet the following criteria as well: (1) age less than 50 years; (2) hemodynamics stable; (3) ICU less than 5 days; (4) Na less than 170 mmol/L or better if less than 150 mmol/L. In 1996 and 1997, the Hamburg group and the UCLA group separately introduced a breakthrough technique performing split liver transplantation in situ. Evidently, the in situ technique has been limited by prolonged time of donor organ procurement , coordination with other organ procurement teams, and even extra burden on donor hospital. Some groups, therefore, have restored the ex situ or bench splitting technique , and fortunately the transplant outcomes of the ex situ technique are equivalent to those of the in situ one. Recently some new techniques have been introduced to split the liver for two adult patients, including the split-cava technique. CONCLUSIONS:It is clear that the most important factor for determining the prognosis of the patient is the time of receiving liver transplantation, not the type of liver transplantation. We still need to pay close attention to the graft to recipient weight ratio (GRWR) and the UNOS classification or MELD score before the patient is subjected to split liver transplantation. | Ji-Qi Yan, Thomas Becker, Cheng-Hong Peng, Hong-Wei Li and Juergen Klempnauer Department of Surgery, Ruijin Hospital Affiliated to Shanghai Second Medical University, Shanghai 200025, China ( Yan JQ, Peng CH and Li HW) Department of Abdominal and Transplant Surgery, Medical School of Hannover, Carl-Neuberg-Str. 1 , 30625 Hannover, Germany | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 3 |
| 6 | The Crosstalk of mTOR/S6K1 and Hedgehog Pathways显示文摘 | Yan Wang Qingqing Ding Chia-Jui Yen Weiya Xia Julie G. Izzo Jing-Yu Lang Chia-Wei Li Jennifer L. Hsu Stephanie A. Miller Xuemei Wang Dung-Fang Lee Jung-Mao Hsu Longfei Huo Adam M. LaBaff Dongping Liu Tzu-Hsuan Huang Chien-Chen Lai Fuu-Jen Tsai Wei-Chao Ch | 2012 | Cancer Cell2012，，3： | 2 |
| 7 | Ceftriaxone-resistant Salmonella enterice serovar hadar:evidence for interspeies transfer of blaCMY-2 in a Taiwanese nuiversity hospital显示文摘 | Yan JJ Chiu CH Ko WC | 2002 | J Formos Med Assoc2002，101，9： | 1 |
| 8 | Spatial and temporal changes of subchondral bone proceed to microscopic articular cartilage degeneration in guinea pigs with spontaneous osteoarthritis 显示文摘 | Wang T Wen C~ Yan CH | 2013 | Osteoarthritis Cartilage2013，21，4： | 1 |
| 9 | Changes in myocardial protein expression in pacing-induced canine heart failure显示文摘 | Heinke MY Wheeler CH Yan JX | 1999 | Electrophoresis1999，20，： | 1 |
| 10 | Sleep problems in chinese school- aged children with a parent-reported history of ADHD 显示文摘 | Li SH Jin XM Yan CH | 2009 | J Atten Disord2009，13，1： | 1 |
| 11 | MicroRNA-31 controls phenotypic modulation of human vascular smooth muscle cells by regulating its target gene cellular repressor of E1A-stimulated genes显示文摘 | Wang J Yan CH Li Y | 2013 | Exp Cell Res2013，319，： | 1 |
| 12 | KISS-1 represses92 kDa type IV collagenase expression by down-regulating NF-kappa B binding to the promoter as a consequence of Ikappa Balpha- induce block o fp65/p50 nuclear translocation显示文摘 | Yan CH Wang H Douglas DB | 2001 | J Biot Chem2001，276，2： | 1 |
| 13 | Ceftriaxone -resistant Salmonella enterica serovar Hadar evidence for interspecies transfer of blaCMY-2 in a Taiwanese university hospital显示文摘 | Yan JJ Chiu CH Ko WC | 2002 | J Formos Med Assoc2002，101，： | 1 |
| 14 | Mutations in the dystrophin gene are associated withsporadic dilated cardiomyopathy显示文摘 | Feng J Yan JY Buzin CH | | 0，，： | 1 |
| 15 | B- and T-lymphocyte attenuator/ herpes virus entry mediator as early indicators for acute rejection following kidney transplantation 显示文摘 | Tian Ch Liu YG Yan JK | 2013 | Transplant Proc2013，45，1： | 1 |
| 16 | Hepatitis B virus basal core promoter mutations A1762T/G1764A are associated with genotype C and a low serum HBsAg level in chroni cally-infected HBeAg positive chinese patients显示文摘 | Yan CH Zhao CY Ding H | 2012 | Antivi ral Res2012，96，2： | 1 |
| 17 | Studies of TGF-beta/Smads expression in lung cancer显示文摘 | Shen JL Yan CH Liu Y | 2003 | Yi Chuan Xue Bao2003，30，7： | 1 |
| 18 | Multiple effects of 2ME2 and D609 on the cortical expression of HIF 1 alpha and apoptotie genes in a middle cerebral artery occlusiowin- duced focal ischemia rat model显示文摘 | Chen CH Hu Q Yan JH | 2007 | J Neurochem2007，102，6： | 1 |
| 19 | Delta-aminolevulinate dehydratase polymorphism and blood lead levels in Chinese children 显示文摘 | Shen XM Wu SH Yan CH | 2001 | Environ Res Sec2001，85，： | 1 |
| 20 | Arsenic trioxide induces autophagic cell death in malignant glioma cells by unregulation or mitochondrial cell deaty protein BNIP3显示文摘 | Yan CH Liang ZQ Gu ZL etal | 2005 | Oncogene2005，24，6： | 1 |
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