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| 1 | In vitro resistance to interferon of hepatitis B virus with precore mutation显示文摘AIM: Chronic hepatitis B virus (HBV) infection is predominantly treated with interferon alpha (IFN-α), which results in an efficient reduction of the viral load only in 20-40% of treated patients. Mutations at HBV precore prevail in different clinical status of HBV infection. The roles of precore mutation in the progression of chronic hepatitis and interferon sensitivity are still unknown. The aim of this study was to explore if there was any relationship between HBV precore mutation and sensitivity to interferon in vitro.METHODS: HBV replication-competent recombinant constructs with different patterns of precore mutations were developed. Then the recombinants were transiently transfected into hepatoma cell line (Huh7) by calcium phosphate transfection method. With or without IFN, viral products in culture medium were collected and quantified 3d after transfection.RESULTS: We obtained 4 recombinant constructs by orientation-cloning 1.2-fold-overlength HBV genome into pUC18 vector via the EcoRI and Hind Ⅲ and PCR mediated site-directed mutagenesis method. All the recombinants contained mutations within precore region. Huh7 cells transfected with recombinants secreted HBsAg and HBV particles into the cell culture medium, indicating that all the recombinants were replication-competent. By comparing the amount of HBV DNA in the medium, we found that HBV DNA in medium reflecting HBV replication efficiency was different in different recombinants. Recombinants containing precore mutation had fewer HBV DNAs in culture medium than wild type. This result showed that recombinants containing precore mutation had lower replication efficiency than wild type. HBV DNA was decreased in pUC18-HBV1.2-WT recombinants after IFN was added while others with precore mutations were not, indicating that HBV harboring precore mutation was less sensitive to IFN in cell culture system.CONCLUSION: These data indicate that HBV harboring precore mutation may be resistant to IFN in vitro. | YanWang LaiWei DongJiang XuCong RanFei JiangXiao YuWang | 2005 | World Journal of Gastroenterology2005，11，5： | 10 |
| 2 | Dendritic cells from chronic hepatitis B patients can induce HBV antigen-specific T cell responses显示文摘AIM: To determine whether dendritic cells (DCs) from chronic hepatitis B patients could induce HBV antigenspecific T cell responses or not. METHODS: DCs were generated from peripheral blood mononuclear cells of patients with chronic hepatitis B (CHB) infection and healthy donors. We compared the phenotypes of these DCs and their ability to secrete cytokines and to participate in mixed lymphocyte reactions. In addition, autologous lymphocytes were cultured with DCs loaded with HBV core region peptide HBcAg8-27, an epitope recognized by cytotoxic T lymphocytes(CTL), and bearing human leucocyte antigen (HLA)-A2 for 10 d. Cytokine secretion and lytic activity against peptide-pulsed target cells were assessed. RESULTS: DCs with typical morphology were generated successfully by culturing peripheral blood mononuclear cells (PBMCs) from CriB patients with AIM-V containing GM-CSF and IL-4. Compared with DCs from normal donors, the level of CD80 expressed in DCs from CHB patients was lower, and DCs from patients had lower capacity of stimulate T cell proliferation. When PBMCs isolated from patients with chronic or acute hepatitis B infection and from normal donors were cocultured with HBcAg18-27 peptide, the antigen-specific memory response of PBMCs from acute hepatitis B patients was stronger than that of PBMCs from chronic hepatitis B patients or normal donors. PBMCs cocultured with DCs treated with HBcAg18-27 CTL epitope peptide induced an antigen-specific T cell reaction, in which the level of secreted cytokines and lyric activity were higher than those produced by memory T cells. CONCLUSION: DCs from patients with CHB can induce HBV antigen-specific T cell reactions, including secretion of cytokines essential for HBV clearance and for killing cells infected with HBV. | Ruo-BingLi Hong-SongChen YaoXie RanFei XuCong DongJiang Song-XiaWang Laiwei YuWang | 2004 | World Journal of Gastroenterology2004，10，11： | 8 |
| 3 | Effect of Temperature on Carbothermic Reduction of Ilmenite显示文摘The reduction of ilmenite (FeTiO3) has been studied extensively. Temperature for the carbothermic reduction of ilmenite ranges from 900 ℃ to 1 400 ℃, and the reduction degree of Panzhihua ilmenite increases with increasing temperature. X-ray diffraction analysis and SEM analysis were used to identify the phase before and after reduction, and to identify the morphology of reduced samples respectively. It is found that the reaction initiates at about 860 ℃. The reaction rate varies with temperature simultaneously. Impurities in Panzhihua ilmenite decrease the reduction degree. Magnesium and calcium oxide-rich zone is formed preventing complete reduction of Fe 2+. In general, the reaction products are iron, Ti3O5 and carbon. | LIWen-bing YUANZhang-fu XUCong PANYi-fang WANGXiao-qiang | 2005 | Journal of Iron and Steel Research(International)2005，12，4： | 7 |
| 4 | Identifi cation and characterization of filamentous fungi isolated from fer- mentation starters for Hong Qu glutinous rice wine brewing显示文摘 | Lv Xucong Huang Zhiqing Ni Li | 2012 | Journal of General and Applied Microbiology2012，58，1： | 1 |
| 5 | Microbial diversity of traditional fermentation starters for Hongqu glu- tinous rice wine as determined by PCR-mediated DGGE显示文摘 | LU Xucong WEN Xing ZHANG Wen | 2012 | Food Control2012，28，2： | 1 |
| 6 | Bacterial community dynamics during the traditional brewing ofWuyi Hong Qu glutinous rice wine as determined by culture-independent methods显示文摘 | LV XUCONG HUANG RUOLAN CHEN FANG | 2013 | Food Control2013，，34： | 1 |
| 7 | Separation of structurally related estrogens using isocratic elution pressurized capillary electrochromatography显示文摘 | Shaofeng Liu Zenghong Xie Xiaoping Wu Xucong Lin Liangqia Guo Guonan Chen | 2005 | Journal of Chromatography A2005，，2： | 1 |
| 8 | Antiviral action of interferon‐α against hepatitis C virus replicon and its modulation by interferon‐γ and interleukin‐8显示文摘 | YintangJia LaiWei DongJiang JianghuaWang XuCong RanFei | 2007 | Journal of Gastroenterology and Hepatology2007，，8： | 1 |
| 9 | Solid phase extraction of lead (Ⅱ),copper (Ⅱ),cadmium (Ⅱ) and nickel (Ⅱ) using gallic acid-modified silica gel prior to determination by flame atomic absorption spectrometry显示文摘 | XIE Fazhi LIN Xucong WU Xiaoping | 2008 | Talanta2008，74，4： | 1 |
| 10 | 显示文摘 | ZHANG Jian WU Xiaoping LIN Xucong | 2007 | J Chromatogr: A2007，1154，12： | 1 |
| 11 | Face detection by structural models 显示文摘 | Yan Junjie Zhang Xucong Eel Zhen | 2013 | Image and Vision Computing2013，32，10： | 1 |
| 12 | 显示文摘 | LIN Jian WU Xiaoping LIN Xucong | 2007 | J Chromatogr: A2007，1169，12： | 1 |
| 13 | Glycin-bonded silica monolithic column as zwitterionic stationary phase for hydrophilic interaction pressurized capillary electrochromatography 显示文摘 | LIN Xucong ZENG Wencan WANG Xiaochun | 2009 | J Sep Sci2009，32，1516： | 1 |
| 14 | Investigation of enantiomer recognition of molecuIarly imprinted polymeric monoliths in pressurized capillary electrochromatography screening the amino acids and their derivatives显示文摘 | LI Min LIN Xucong XIE Zenghong | 2009 | Journal of Chromatography A2009，1216，27： | 1 |
| 15 | Pharmacokinetics of ciprofloxacin in eels by high-perf-ormance liquid chromatography with fluorescence detection 显示文摘 | Guo Liangqia Xie Zenghong Lin Xucong | 2005 | Analytical Biochemistry2005，341，2： | 1 |
| 16 | Bacterial community dynamics during the traditional brewing of Wuyi Hong Qu glutinous rice wine as determined by culture-independent methods 显示文摘 | Xucong Lv Ruolan Huang Fang Chen | 2013 | Food Control2013，34，： | 1 |
| 17 | Electroneutral silica -based hybrid monolith for hydrophilic interaction capil- lary electrochromatography 显示文摘 | Lin Xucong Wang Xiao Zhao Tingting | 2012 | Journal of Chromatography A2012，1260，： | 1 |
| 18 | Microbial diversity of traditional fermentation starters for Hong Qu glutinous rice wine as determined by PCR-mediated DGGE显示文摘 | Lv Xucong Weng Xing Zhang Wen | 2012 | Food Control2012，28，2： | 1 |
| 19 | Pharmacokinetics of ciprofloxacin in eels by high-performance liquid chromatography with fluorescence detection显示文摘 | GUO Liangqia XIE Zenghong LIN Xucong | 2005 | Analytical Biochemistry2005，341，2： | 1 |
| 20 | Microbial diversity of traditional fermentation starters for Hong Qu glutinous ricewine as determined by PCR-mediated DGGE显示文摘 | Lv Xucong Weng Xing Zhang Wen | 2012 | Food Control2012，28，2： | 1 |
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