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| 1 | Ginsenoside Rgl protects against ischemic/reperfusioninduced neuronal injury through miR-144/Nrf2/ARE pathway显示文摘Ginsenoside Rg1 (Rg1),a saponin extracted from Panax ginseng,has been well documented to be effective against ischemic/ reperfusion (I/R)neuronal injury.However,the underlying mechanisms remain obscure.In the present study,we investigated the roles of Nrf2 and miR-144 in the protective effects of Rgl against I/R-induced neuronal injury.In OGD/R-treated PC12 cells,Rgl (0.01-1 μmol/L)dose-dependently attenuated the cell injury accompanied by prolonging nuclear accumulation of Nrf2,enhancing the transcriptional activity of Nrf2,as well as promoting the expression of ARE-target genes.The activation of the Nrf2/ARE pathway by Rgl was independent of disassociation with Keapl,but resulted from post-translational regulations.Knockdown of Nrf2 abolished all the protective changes of Rgl in OG-D/R-treated PC12 cells.Furthermore,Rgl treatment significantly decreased the expression of miR-144,which downregulated Nrf2 production by targeting its 3'-untranlated region after OGD/R.Knockdown of Nrf2 had no effect on the expression of miR-144,suggesting that miR-144 was an upstream regulator of Nrf2.We revealed that there was a direct binding between Nrf2 and miR-144 in PC12 cells.Application of anti-miR-144 occluded the activation of the Nrf2/ ARE pathway by Rgl in OGD/R-treated PC12 cells.In tMCAO rats,administration of Rgl (20 mg/kg).significantly alleviated ischemic injury,and activated Nff2/ARE pathway.The protective effects of Rgl were abolished by injecting of AAV-HIF-miR-144-shRNA into the predicted ischemic penumbra.In conclusion,our results demonstrate that Rgl alleviates oxidative,stress after I/R through inhibiting miR-144 activity and subsequently promoting the Nrf2/ARE pathway at the post-translational-level. | Shi-feng Chu Zhao Zhang Xin Zhou Wen-bin He Chen Chen Piao Luo Dan-dan Liu Qi-di Ai Hai-fan Gong Zhen-zhen Wang Hong-shuo Sun Zhong-ping Feng Nai-hong Chen | 2019 | Acta Pharmacologica Sinica2019，40，1： | 30 |
| 2 | The Association of Maternal Body Composition and Dietary Intake with the Risk of Gestational Diabetes Mellitus during the Second Trimester in a Cohort of Chinese Pregnant Women显示文摘Objective To investigate the association of maternal body composition and dietary intake with the risk of gestational diabetes mellitus(GDM). Methods A total 154 GDM subjects and 981 controls were enrolled in a prospective cohort study in 11 hospitals from May 20, 2012 to December 31, 2013. Bioelectrical impedance analysis and dietary surveys were used to determine body composition and to evaluate the intake of nutrients in subjects at 21-24 weeks' gestation(WG). Logistic regression analysis was applied to explore the relationships of maternal body composition and dietary intake with the risk of GDM morbidity. Results Age, pre-pregnant body weight(BW), and body mass index(BMI) were associated with increased risk of GDM. Fat mass(FM), fat mass percentage(FMP), extracellular water(ECW), BMI, BW, energy, protein, fat, and carbohydrates at 21-24 WG were associated with an increased risk of GDM. In contrast, fat free mass(FFM), muscular mass(MM), and intracellular water(ICW) were associated with a decreased risk of GDM. Conclusion Maternal body composition and dietary intake during the second trimester of pregnancy were associated with the risk of GDM morbidity. | Xu Qing Gao Zhi Ying Li Li Ming Wang Lu Zhang Qian Teng Yue Zhao Xia Ge Sheng Jing Hong Jiang Yang Yong Tao Liu Xiao Jun Lyu Chun Jian Mao Lun Yu Xiao Ming Liu Ying Hua Kong Ai Jing Yang Xue Yan Liu Zhao Zhang Yong Wang Jin Zhang Xin Sheng Xue Chang Yong Lu Yan Ping | 2016 | Biomedical and Environmental Sciences2016，29，1： | 29 |
| 3 | Chinese Multidisciplinary Expert Consensus on the Diagnosis and Treatment of Hyperuricemia and Related Diseases显示文摘 | Zou, He-Jian Wu, Hu-Sheng Zhou, Jing-Guo Zeng, Xue-Jun Dai, Lie Wu, Hua-Xiang Zhu, Xiao-Xia Mei, Chang-Lin Hao, Chuan-Ming Chen, Nan Liu, Bi-Cheng Chen, Jiang-Hua Yang, Li Nie, Jing Yu, Chen Peng, Ai Yu, Sheng-Qiang Li, Lin Ge, Jun-Bo Huo, Yong Zhang, Shu-Yang Chen, Yun-Dai Dong, Yu-Gang Liang, Chun Dai, Yu-Xiang Gao, Xin Li, Chang-Gui Zhao, Jia-Jun Chen, Hai-Bing Cheng, Zhi-Feng Lin, Huan-Dong Guan, Yang-Tai Wang, Kai Luo, Ben-Yan Dai, Ruo-Lian Jiang, Quan Xue, Luan Liang, Chao-Chao Chen, Ming Fan, Song | 2017 | Chinese Medical Journal2017，，20： | 30 |
| 4 | Genotypic Characterization of Methicillin-resistant Staphylococcus aureus Isolated from Pigs and Retail Foods in China显示文摘Objective To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus(MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. Methods Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing(MLST), spa typing, multiple-locus variable number of tandem repeat analysis(MLVA), pulsed-field gel electrophoresis(PFGE), and antimicrobial susceptibility testing. Results All isolated MRSA exhibited multi-drug resistance(MDR). Greater diversity was found in food-associated MRSA(7 STs, 8 spa types, and 10 MLVA patterns) compared to pig-associated MRSA(3 STs, 1 spa type, and 6 MLVA patterns). PFGE patterns were more diverse for pig-associated MRSA than those of food-associated isolates(40 vs. 11 pulse types). Among the pig-associated isolates, CC9-ST9-t899-MC2236 was the most prevalent clone(96.4%), and CC9-ST9-t437-MC621(20.0%) was the predominant clone among the food-associated isolates. The CC9-ST9 isolates showed significantly higher antimicrobial resistance than other clones. Interestingly, CC398-ST398-t034 clone was identified from both pig-and food-associated isolates. Of note, some community-and hospital-associated MRSA strains(t030, t172, t1244, and t4549) were also identified as food-associated isolates. Conclusion CC9-ST9-t899-MC2236-MDR was the most predominant clone in pigs, but significant genetic diversity was observed in food-associated MRSA. Our results demonstrate the great need for improved surveillance of MRSA in livestock and food and effective prevention strategies to limit MDR-MRSA infections in China. | WANG Wei LIU Feng ZULQARNAIN Baloch ZHANG Cun Shan MA Ke PENG Zi Xin YAN Shao Fei HU Yu Jie GAN Xin DONG Yin Ping BAI Yao LI Feng Qin YAN Xiao Mei MA Ai Guo XU Jin | 2017 | Biomedical and Environmental Sciences2017，30，8： | 13 |
| 5 | Research on large-scale dispatchable grid-connected PV systems显示文摘This paper describes the research on a largescale dispatchable grid-connected photovoltaic(PV)system for supplying power to the grid for dispatch instead of supplying the electricity to a local load.In order to maximise the value of the solar energy,a hybrid electricity storage consisting of batteries and supercapacitors is used with the PV system.This paper proposes a control strategy focusing on theDCpower at theDClink rather than at the grid-connected inverter.Two typical sets of real data,collected from existing sites,are used to demonstrate the practicality of the system.Finally,the simulation results are used to demonstrate the good performance and feasibility of the proposed system together with the proposed control strategy. | Xiaonan HAN Xin AI Yingyun SUN | 2014 | Journal of Modern Power Systems and Clean Energy2014，2，1： | 12 |
| 6 | Water relations and an expression analysis of plasma membrane intrinsic proteins in sensitive and tolerant rice during chilling and recovery显示文摘寒心的损害的症状是在射击的水赤字的开发,源于水运输和蒸发的不平衡。在这个工作,二个米饭变化(Oryza sativa L。var。Wasetoitsu 和 Somewake ) 幼苗是在 7 度 C 的 chilled,在 28 度 C 由恢复列在后面。基于生长显型和电解质漏测试, Somewake 被显示是一个寒心容忍的变化,和 Wasetoitsu 寒心敏感的。寒心的压力显著地减少了叶子,积聚的蒸发和渗透的根的相对的水内容(RWC ) 在两个变化的水力的电导率(Lp ) 。但是当回到了 28 度 C 时, Somewake 的水关系平衡更好恢复了。所有 11 血浆膜的 mRNA 表示侧面内在的蛋白质(果仁) , aquaporins 的亚群,被有 TaqMan 次要的林子文件夹(MGB ) 的 -PCR 探查的即时反向的抄写(RT ) 随后决定源于米饭 var。Nipponbare 在寒心的处理和恢复期间。大多数 PIP 基因在低温度是下面调整的,并且在温暖的温度恢复了。在 Somewake 和 Wasetoitsu 的一些果仁的相对表示在平行减少了在期间寒心。然而在恢复期间, OsPIP1 的相对表示; 1, OsPIP2; 1, OsPIP2; 7 在射击和 OsPIP1; 1, OsPIP2; 1 比 Wasetoitsu 在 Somewake 在根是显著地更高的。这在在寒心的条件以后重建水平衡支持果仁的角色。我们讨论 aquaporin 果仁亚科的成员在植物起的多样化的作用寒心的忍耐取决于 aquaporin isoforms,植物织物和寒心的持续时间的阶段。 | Xin Yu Yan Hui Peng Min Hua Zhang Yan Jun Shao Wei Ai Su Zhang Cheng Tang | 2006 | Cell Research2006，16，6： | 11 |
| 7 | The effect of nanohybrid materials on the pour-point and viscosity depressing of waxy crude oil显示文摘The pipeline transportation of waxy crude oil is a problem both at home and abroad.In this paper,a novel nanohybrid pour-point depressant (PPD) was used to decrease the pour point and viscosity of waxy crude oil.The pour point and apparent viscosity of waxy crude oil was decreased significantly upon addition of the nanohybrid PPD,and the long-term stability of the nanohybrid PPD was superior to that of a conventional ethylene-vinyl acetate copolymer PPD.Polarized optical microscopy and X-ray diffraction were used to study the effect of the nanohybrid PPD on the crystallization of crude oil.Addition of the nanohybrid PPD reduced the amount of wax crystals,prevented their aggregation,and reduced the temperature at which the crude oil started to crystallize.The significant effect of this nanohybrid PPD on the pour point and viscosity depressing of crude oil is of great importance for facilitating the safe,efficient and energy-minimized transportation of waxy crude oil. | WANG Feng ZHANG DongMin DING YanFen ZHANG LiXin YANG MingShu JIANG BaoLiang ZHANG ShiMin AI MuYang LIU GuangWen ZHI ShuJie HUO LianFeng OUYANG Xin LI Li | 2011 | Chinese Science Bulletin2011，56，1： | 9 |
| 8 | Runoff reduction due to environmental changes in the Sanchuanhe river basin显示文摘Recently,runoff in many river basins in China has been decreasing.Therefore,the role that climate change and human activities are playing in this decrease is currently of interest.In this study,we evaluated an assessment method that was designed to quantitatively separate the effects of climate change and human activities on runoff in river basins.Specifically,we calibrated the SIMHYD rainfall runoff model using naturally recorded hydro-meteorologic data pertaining to the Sanchuanhe River basin and then determined the effects of climate change and human activities on runoff by comparing the estimated natural runoff that occurred during the period in which humans disturbed the basin to the runoff that occurred during the period prior to disturbance by humans.The results of this study revealed that the SIMHYD rainfall runoff model performs well for estimating monthly discharge.In addition,we found that absolute runoff reductions have increased in response to human activities and climate change,with average reductions of 70.1% and 29.9% in total runoff being caused by human activities and climate change,respectively.Taken together,the results of this study indicate that human activities are the primary cause of runoff reduction in the Sanchuanhe River basin. | Guoqing WANG Jianyun ZHANG Ruimin HE Naiqian JIANG Xin'ai JING | 2008 | International Journal of Sediment Research2008，23，2： | 9 |
| 9 | Association between Plasma Metal Levels and Diabetes Risk: a Case-control Study in China显示文摘Objective Many metals, some of which have been classified as environmental endocrine disruptors, are used extensively in everyday consumer products and are ubiquitous in our living environment. In the present study, we aimed to explore the associations between the prevalence risk of type 2 diabetes and plasma levels of 20 trace elements as well as those of heavy metals in a Han Chinese population. Methods We conducted a case-control study to investigate the associations between plasma concentrations of 20 metals and diabetes in Jiangsu province. A total of 122 newly diagnosed cases of type 2 diabetes and 429 matched controls were recruited from community physical examinations in Suzhou City of Jiangsu Province. Plasma metal levels were measured by inductively-coupled plasma mass spectrometry. Results After adjusting for confounders, plasma vanadium, chromium, manganese, copper, zinc, arsenic, selenium, strontium, palladium, cadmium, cesium, and barium were associated with diabetes risk(P < 0.05). The adjusted OR increased with increasing concentration of vanadium, manganese, copper, zinc, and cesium. Conclusion Many metals, including manganese, copper, zinc, arsenic, selenium, and cadmium in plasma, are associated with the morbidity of diabetes. Monitoring of environmental metal levels and further studies are urgently needed. | LI Xiu Ting YU Peng Fei GAO Yan GUO Wen Hui WANG Jun LIU Xin GU Ai Hua JI Gui Xiang DONG Qiu WANG Bo Shen CAO Ying ZHU Bao Li XIAO Hang | 2017 | Biomedical and Environmental Sciences2017，30，7： | 8 |
| 10 | Ursodeoxycholic acid protects interstitial Cajal-like cells in the gallbladder from undergoing apoptosis by inhibiting TNF-α expression显示文摘运动不足是胆石疾病的普通症状,它被空隙的象 Cajal 一样房间(ICLC ) 的损失在胆囊伴随。Ursodeoxycholic 酸(UDCA ) 广泛地在治疗胆石疾病被使用,并且除了它溶解胆石的能力显示出 anti-apoptotic 和反煽动性的效果。在这研究,我们与胆石在畿尼猪在 ICLC 上调查了 anti-apoptotic 和 UDCA 的反煽动性的效果。畿尼猪被喂为 8 个星期到的一本高胆固醇的食谱导致胆石的形成。一组动物被管理 UDCA (在跟随 553 的 consultation.ResultsA 总数的临床的管理的一个变化请教的 50 mgith 为 268 个婴儿被执行(gestational 年龄:在到控制的 CP 和 RAP.ResultsCompared 之间的 27ffect QOL 预言者(51.0ook 建筑群产品,它证明 GaoFen-3 是 SAR 图象在有另外的二幅 C 乐队 SAR 图象和它 3.5 dB 的 RR 会系统设计的一样的优秀水平。然后,这些 thr 的反散射系数?? | Jiang-fan WAN Shi-feng CHU Xin ZHOU Yue-ting LI Wen-bin HE Feng TAN Piao LUO Qi-di AI Qi WANG Nai-hong CHEN | 2018 | Acta Pharmacologica Sinica2018，39，9： | 8 |
| 11 | Fault location of distribution networks based on multi-source information显示文摘In order to promote the development of the Internet of Things(IoT),there has been an increase in the coverage of the customer electric information acquisition system(CEIAS).The traditional fault location method for the distribution network only considers the information reported by the Feeder Terminal Unit(FTU)and the fault tolerance rate is low when the information is omitted or misreported.Therefore,this study considers the influence of the distributed generations(DGs)for the distribution network.This takes the CEIAS as a redundant information source and solves the model by applying a binary particle swarm optimization algorithm(BPSO).The improved Dempster/S-hafer evidence theory(D-S evidence theory)is used for evidence fusion to achieve the fault section location for the distribution network.An example is provided to verify that the proposed method can achieve single or multiple fault locations with a higher fault tolerance. | Wenbo Li Jianjun Su Xin Wang Jiamei Li Qian Ai | 2020 | Global Energy Interconnection2020，3，1： | 7 |
| 12 | Identification of AtENT3 as the main transporter for uridine uptake in Arabidopsis roots显示文摘当在酵母细胞生产了,差别在 Arabidopsis 器官被表示时,以前的研究证明了 Arabidopsis equilibrative nucleoside 运输 ers (AtENTs ) 拥有运输活动。此处,我们在 nucleoside 运输活动和 AtENT 成员的 transcriptional 模式报导进一步的分析。AtENTs 3 的 recombinant 蛋白质, 6,和 7,然而并非那些 AtENTs 1, 2, 4,和 8,被发现与高亲密关系搬运 thymidine。与以前的建议相反,那 AtENT1 不能搬运 uridine,这个工作证明 recombinant AtENT1 是 uridine 的 pH 依赖、高亲密关系的 transporter。当在 MS 盘子上成长时, AtENT3 猛烈植物比野类型的植物和 AtENT1 或 AtENT6 的猛烈植物对细胞毒素的 uridine 类似物 5-fluorouridine 更容忍。与这观察一致, AtENT3 猛烈的线展出了一个显著地减少的能力收起[(3 ) H ] 经由根的 uridine 什么时候与有变异的 AtENT1 或 AtENT6 的野类型的植物和植物相比。这显示那 AtENT3,然而并非 AtENTs 1 并且 6,是为在 Arabidopsis 根的 uridine 举起的主要 transporter。AtENTs 1 的抄写, 3, 4, 6, 7,和 8 在叶开发和老朽期间以一种复杂方式被调整。相反,六个 AtENT 成员并列地在种子萌芽期间被劝诱。这个工作为未来研究在 recombinant AtENT 蛋白质和新线索的运输性质上提供新信息在里面 vivo 运输活动和在 Arabidopsis 工厂的不同 ENT 蛋白质的生理的功能。 | Kun Ling Chen Min Xin Xu Guang Yong Li Hui Liang Zong Liang Xia Xin Liu Ji Shu Zhang Ai Min Zhang Dao Wen Wang | 2006 | Cell Research2006，16，4： | 7 |
| 13 | Mammalian cell display for rapid screening scFv antibody therapy显示文摘人的抗体开始在有免疫力的基因治疗为使用正在引起注意。对癌症和传染疾病的治疗合适的有效治疗学的 monoclonal 抗体的有效产生将是极其珍贵的。抗体显示方法逐渐地被用来屏蔽人的 monoclonal 抗体。这里,我们报导一个哺乳动物的房间显示方法的构造源于一个天真的抗体全部剧目,为人,单个链的变量碎裂短暂地基于 pDisplay 向量在 293T 房间表面上被显示了。当前的 pDisplayscFv 抗体全部剧目的尺寸被估计了是 0. | Jing Zhang Xiao'ai Zhang Qiang Liu Mengyi Li Liucun Gao Xin Gao Shensi Xiang Liangliang Wu Jie Fu Haifeng Song | 2014 | Acta Biochimica et Biophysica Sinica2014，46，10： | 6 |
| 14 | Multifunctional dextran micelles as drug delivery carriers and magnetic resonance imaging probes显示文摘Multifunctional nanoparticles combining diagnostic and therapeutic agents into a single platform make cancer theranostics possible and have attracted wide interests in the field. In this study, a multifunctional nanocomposite based on dextran and superparamagnetic iron oxide nanoparticles(SPIO) was prepared for drug delivery and magnetic resonance imaging(MRI). Amphiphilic dextran was synthesized by grafting stearyl acid onto the carbohydrate backbone, and micelle was formed by the resulted amphiphilic dextran with low critical micelle concentration at 1.8 mg L-1. Doxorubicin(DOX) and a cluster of the manganese-doped iron oxide nanoparticles(Mn-SPIO)nanocrystals were then coencapsulated successfully inside the core of dextran micelles, resulting in nanocomposites with diameter at about 100 nm. Cell culture experiments demonstrated the potential of these Mn-SPIO/DOX nanocomposites as an effective multifunctional nanoplatform for the delivery of anticancer drug DOX with a loading content(DLC) of 16 %. Confocal laser scanning microscopyreveals that the Mn-SPIO/DOX had excellent internalization ability against MCF-7/Adr cells after 2-h labeling compared with free DOXáHCl. Under a 3.0-T MRI scanner, Mn-SPIO/DOX nanocomposite-labeled cells in gelatin phantom show much darker images than the control. Their transverse relaxation(T2) rate is also significantly higher than that of the control cells(33.9 versus 2.3 s-1). Our result offers an effective strategy to treat MCF-7/Adr at optimized low dosages with imaging capability. | Bingbing Lin Hongying Su Rongrong Jin Danyang Li Changqiang Wu Xin Jiang Chunchao Xia Qiyong Gong Bin Song Hua Ai | 2015 | Science Bulletin2015，60，14： | 6 |
| 15 | Serum Gamma-glutamyl Transferase Levels Predict Functional Outcomes after Aneurysmal Subarachnoid Hemorrhage显示文摘Objective We aim to explore the potential association between serum gamma-glutamyl transferase levels and functional outcome after aneurysmal subarachnoid hemorrhage in a Chinese population. Methods A total of 386 aneurysmal subarachnoid hemorrhage patients were included in the study from September 2007 to February 2015. Baseline serum gamma-glutamyl transferase levels and 6-month follow-up functional outcomes were determined. A poor outcome was defined as a modified ranking scale score of ≥ 3. The multivariable logistic model was used to analyze the relationship between serum gamma-glutamyl transferase and clinical outcomes after aneurysmal subarachnoid hemorrhage. Results The adjusted poor outcome rates of patients with gamma-glutamyl transferase levels of < 30 U/L, 30-50 U/L and ≥ 50 U/L were 16.7%, 19.6%, and 34.4%, respectively(P < 0.01). The age-sex and multivariable adjusted odds ratios(95% confidence intervals) of poor prognosis comparing the top group(≥ 50 U/L) with the lowest group(< 30 U/L) were 5.76(2.74-12.13), 6.64(2.05-21.52), and 6.36(1.92-21.02). A significant linear trend existed between gamma-glutamyl transferase level and aneurysmal subarachnoid hemorrhage prognosis. This association was also observed among nondrinkers. Conclusion Patients with higher gamma-glutamyl transferase levels were more likely to have a poor prognosis. Serum gamma-glutamyl transferase can be considered to be an independent predictor of functional outcomes after aneurysmal subarachnoid hemorrhage. | XU Tian WANG Wei ZHAI Lin ZHANG Yun Feng ZHOU Hong Zhi WU Xin Min LI Ai Hong XIE Li Li NING Xiao Jin JI Yu Teng WANG Hong Mei KE Kai Fu | 2017 | Biomedical and Environmental Sciences2017，30，3： | 5 |
| 16 | Contribution of aboveground litter to soil respiration in Populus davidiana Dode plantations at different stand ages显示文摘Soil respiration from decomposing aboveground litter is a major component of the terrestrial carbon cycle.However,variations in the contribution of aboveground litter to the total soil respiration for stands of varying ages are poorly understood.To assess soil respiration induced by aboveground litter,treatments of litter and no litter were applied to 5-,10-,and 20-year-old stands of Populus davidiana Dode in the sandstorm source area of Beijing -Tianjin,China.Optimal nonlinear equations were applied to model the combined effects of soil temperature and soil water content on soil respiration.Results showed that the monthly average contribution of aboveground litter to total soil respiration were 18.46% ± 4.63%,16.64% ± 9.31%,and 22.37% ± 8.17% for 5-,10-,and 20-year-old stands,respectively.The relatively high contribution in 5-and 20-year-old stands could be attributed to easily decomposition products and high accumulated litter,respectively.Also,it fluctuated monthly for all stand ages due to substrate availability caused by phenology and environmental factors.Litter removal significantly decreased soil respiration and soil water content for all stand ages(p < 0.05) but not soil temperature(p > 0.05).Variations of soil respiration could be explained by soil temperature at 5-cm depth using an exponential equation and by soil water content at 10-cm depth using a quadratic equation,whereas soil respiration was better modeled using the combined parameters of soil temperature and soil water content than with either soil temperature or soil water content alone.Temperature sensitivity(Q_(10))increased with stand age in both the litter and the no litter treatments.Considering the effects of aboveground litter,this study provides insights for predicting future soil carbon fluxes and for accurately assessing soil carbon budgets. | ZHAO Xin LI Fa-dong ZHANG Wan-jun AI Zhi-pin | 2016 | Journal of Mountain Science2016，13，6： | 5 |
| 17 | Epidermal growth factor receptor mutation analysis in cytological specimens and responsiveness to gefitinib in advanced non-small cell lung cancer patients显示文摘Background: Epidermal growth factor receptor(EGFR) mutation is the key predictor of EGFR tyrosine kinase inhibitors(TKIs) efficacy in non-small cell lung cancer(NSCLC). We conducted this study to verify the feasibility of EGFR mutation analysis in cytological specimens and investigate the responsiveness to gefitinib treatment in patients carrying EGFR mutations.Methods: A total of 210 cytological specimens were collected for EGFR mutation detection by both direct sequencing and amplification refractory mutation system(ARMS). We analyzed EGFR mutation status by both methods and evaluated the responsiveness to gefitinib treatment in patients harboring EGFR mutations by overall response rate(ORR), disease control rate(DCR) and progression free survival(PFS).Results: Of all patients, EGFR mutation rate was 28.6%(60/210) by direct sequencing and 45.2%(95/210) by ARMS(P<0.001) respectively. Among the EGFR wild type patients tested by direct sequencing, 26.7% of them were positive by ARMS. For the 72 EGFR mutation positive patients treated with gefitinib, the ORR, DCR and median PFS were 69.4%, 90.2% and 9.3 months respectively. The patients whose EGFR mutation status was negative by direct sequencing but positive by ARMS had lower ORR(48.0% vs. 80.9%, P=0.004) and shorter median PFS(7.4 vs. 10.5 months, P=0.009) as compared with that of EGFR mutation positive patients by both detection methods. Conclusions: Our study verified the feasibility of EGFR analysis in cytological specimens in advanced NSCLC. ARMS is more sensitive than direct sequencing in EGFR mutation detection. EGFR Mutation status tested on cytological samples is applicable for predicting the response to gefitinib. Abundance of EGFR mutations might have an influence on TKIs efficacy. | Lin Li Zijin Zhang Zhixin Bie Zheng Wang Ping Zhang Xin Nie Yuanming Li Hui Wang Bin Ai Gang Cheng | 2015 | Chinese Journal of Cancer Research2015，27，3： | 5 |
| 18 | A new method for rating dyspnea during exercise in patients with chronic obstructive pulmonary disease显示文摘 | WANG Hao-yan XU Qiu-fen YUAN Wei NIE Shan HE Xin ZHANG Jian KONG Yuan-yuan AI Sperry David Guan | 2013 | Chinese Medical Journal2013，，19： | 5 |
| 19 | CZ-7, a new derivative of Claulansine F, ameliorates 2V0-induced vascular dementia in rats through a Nrf2-mediated antioxidant responses显示文摘Vascular dementia (VD) results from accumulated damage in the vascular system, which is characterized by progressive impairments in memory and cognition and is second only to Alzheimer’s disease (AD) in prevalence among all types of dementia. In contrast to AD, there is no FDA-approved treatment for VD owing to its multiple etiologies. In this study, we investigated whether CZ-7, a new derivative of Claulansine F (Clau F) with verified neuroprotective activity in vitro, could ameliorate the cognitive impairment of rats with permanent occlusion of bilateral common carotid arteries (2VO) and its potential mechanisms of action. The 2VO rats were orally administered CZ-7 (10, 20, 40?mg/kg) from day 27 to day 53 post-surgery. Morris water maze tests conducted at day 48–51 revealed that CZ-7 administration significantly reduced the escape latency in 2VO rats. After the rats were sacrificed on day 53, morphological studies using Nissl and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining showed that administration of CZ-7 markedly attenuated the pathological changes in CA1–CA3 area of the hippocampus, including neuronal cell loss, nuclear shrinkage, and dark staining of neurons, and significantly decreased the chronic cerebral hypoperfusion-induced cell loss. Klüver–Barrera staining study revealed that CZ-7 administration significantly improved the white matter lesions. 8-OHdG and reactive oxygen species (ROS) immunofluorescent analyses showed that CZ-7 administration significantly decreased oxidative stress in CA1–CA3 area of the hippocampus. Finally, we found that the CZ-7-improved oxidative stress might be mediated via the Nrf2 pathway, evidenced by the double immunofluorescent staining of Nrf2 and the elevation of expression levels of oxidative stress proteins HO-1 and NQO1. In conclusion, CZ-7 has therapeutic potential for VD by alleviating oxidative stress injury through Nrf2-mediated antioxidant responses. | Dan-dan Liu Xia Yuan Shi-feng Chu Chen Chen Qian Ren Piao Luo Mei-yu Lin Sha-sha Wang Tian-bi Zhu Qi-di Ai Ying-da Zang Dong-ming Zhang Xin He Zhi-hua Huang Hong-shuo Sun Zhong-ping Feng Nai-hong Chen | 2019 | Acta Pharmacologica Sinica2019，40，4： | 4 |
| 20 | Phase I safety and pharmacokinetic study of bevacizumab in Chinese patients with advanced cancer显示文摘背景 bevacizumab 是人性化的 recombinant 脉管的 endothelial 生长因素(VEGF ) monoclonal 抗体,明确地绑在 VEGF 并且禁止肿瘤细胞生长,增长和转移。我们试图与先进癌症在中国病人调查 bevacizumab 的安全和 pharmacokinetics。方法 39 个中国病人与变形或 relapsed 癌症没有通过优先的治疗的人,在我 bevacizumab 学习的这个阶段被注册。Bevacizumab 被计算的泵在 90 分钟从 5 mg/kg 在剂量灌输到 15 mg/kg。病人们经历了连续 pharmacokinetic 评估。收到了 bevacizumab 的至少一注入的病人在 35 个病人都完成了 5 注入列在后面的安全 study.Results 被包括协议。一个病人由于等级 3 proteinuria 在 3 以后撤退了注入。普通不利事件可能与学习药有关是 proteinuria ( 17/39 ,43.6%),高血压( 13/39 ,33.3%),齿龈的流血( 7/39 ,17.9%), epistaxis ( 6/39 ,15.4%),咽头的发炎( 6/39 ,15.4%),疲劳(6/39,15.4%)和口炎(4/39,10.3%)。Bevacizumab pharmacokinetics 在 5 mg/kg q2w-10 mg/kg q2w 和 15 mg/kg q3w 的范围以内是线性的。CL (清理) , Vd (在消除的分发的体积) 和 Vss (以稳定的状态的 diatribution 的体积) 在在 5,10 和 15 mg/kg 的单个、多重的剂量以后是类似的。结论 Bevacizumab 很好在中国病人被容忍。没有意外不利事件被观察。在 pharmacokinetics 没有种族差别。 | WU Jian-yu WU Xiao-nan DING Li ZHAO Yun-bo AI Bin LI Yang HU Xin CHENG Gang | 2010 | Chinese Medical Journal2010，，7： | 4 |
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