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| 1 | Clarifying sub-genomic positions of QTLs for flowering habit and fruit quality in U.S. strawberry (Fragaria×ananassa) breeding populations using pedigree-based QTL analysis显示文摘The cultivated strawberry(Fragaria×ananassa)is consumed worldwide for its flavor and nutritional benefits.Genetic analysis of commercially important traits in strawberry are important for the development of breeding methods and tools for this species.Although several quantitative trait loci(QTL)have been previously detected for fruit quality and flowering traits using low-density genetic maps,clarity on the sub-genomic locations of these QTLs was missing.Recent discoveries in allo-octoploid strawberry genomics led to the development of the IStraw90 single-nucleotide polymorphism(SNP)array,enabling high-density genetic maps and finer resolution QTL analysis.In this study,breeder-specified traits were evaluated in the Eastern(Michigan)and Western(Oregon)United States for a common set of breeding populations during 2 years.Several QTLs were validated for soluble solids content(SSC),fruit weight(FWT),pH and titratable acidity(TA)using a pedigree-based QTL analysis approach.For fruit quality,a QTL for SSC on linkage group(LG)6A,a QTL for FWT on LG 2BII,a QTL for pH on LG 4CII and two QTLs for TA on LGs 2A and 5B were detected.In addition,a large-effect QTL for flowering was detected at the distal end of LG 4A,coinciding with the FaPFRU locus.Marker haplotype analysis in the FaPFRU region indicated that the homozygous recessive genotype was highly predictive of seasonal flowering.SNP probes in the FaPFRU region may help facilitate marker-assisted selection for this trait. | Sujeet Verma Jason D Zurn Natalia Salinas Megan M Mathey Beatrice Denoyes James F Hancock Chad E Finn Nahla V Bassil Vance M Whitaker | 2017 | Horticulture Research2017，4，1： | 10 |
| 2 | Characterization of spermidine hydroxycinnamoyl transferases from eggplant (Solanum melongena L.) and its wild relative Solanum richardii Dunal显示文摘Eggplant produces a variety of hydroxycinnamic acid amides(HCAAs)that have an important role in plant development and adaptation to environmental changes.In this study,we identified and characterized a spermidine hydroxycinnamoyl transferase(SHT)from eggplant(Solanum melongena)and its wild relative S.richardii,designated as SmSHT and SrSHT,respectively.SmSHT was abundant in flowers and fruits,whereas the level of SrSHT was remarkably low in all tissues.Heat-shock/drought treatment stimulated the expression of SmSHT in both leaves and fruits,indicating its involvement in plant stress response.Both SHT polypeptides had extremely high identity with just five amino-acid substitutions.Recombinant SmSHT catalyzed the synthesis of mono-,bi-and tri-acylated polyamines.Using caffeoyl-CoA as the acyl donor,SmSHT preferred spermidine as the acyl acceptor.When spermidine was the acyl acceptor,the donor preference order for SmSHT was caffeoyl-CoA>feruloyl-CoA>ρ-coumaroyl-CoA.SrSHT exhibited the same substrate specificity as SmSHT,yet exhibited significantly higher catalytic activity than SmSHT.For example,under caffeoyl-CoA and spermidine,Kcat of SrSHT was 37.3%higher than SmSHT.Molecular modeling suggests that five amino-acid substitutions in SrSHT result in four alterations in their predicted 3D structures.In particular,the conserved Lys402 adjacent to the DFGWG motif,and Cys200 in the crossover loop in SmSHT were replaced by Glu and Ser in SrSHT.These substitutions may contribute to the enhanced activity in SrSHT.Our study provides a platform to generate HCAA rich fruits for eggplant and other solanaceous crops. | Hui Peng Tianbao Yang Bruce D Whitaker Frances Trouth Lingfei Shangguan Wen Dong Wayne M Jurick | 2016 | Horticulture Research2016，3，1： | 2 |
| 3 | The dynamic behavior of transformer winding under axial short-circuit forces显示文摘 | Madin A B Whitaker J D | 1963 | Proceedings IEE1963，110，3： | 2 |
| 4 | Dection of anticentromere antibodies using cloned autoantigen CENP-B显示文摘 | Whitaker D Bordwell B | 1987 | Athritis Rheum1987，30，12： | 1 |
| 5 | The reverse Warburg effect: glyeolysis inhibitors prevent the tumor promoting effects of caveolin - 1 deficient cancer associated fibro- blasts显示文摘 | Bonucce|li G Whitaker - Menezes D Castello - Cros R | 2010 | Cell Cycle2010，9，10： | 1 |
| 6 | Hirudo medicinalis:ancientorigins of and trends in the use of medicinal leeches throughouthistory 显示文摘 | Whitaker I S Rao J Izadi D | 2004 | British J Oral Maxill Surg2004，42，2： | 1 |
| 7 | Phytosterols, phytostanols, and their conjugates in foods: structural diversity, quantitative analysis, and health-promoting uses显示文摘 | A Robert Moreau D Bruce Whitaker B Kevin Hicks | 2002 | Progress in Lipid Research2002，，41： | 1 |
| 8 | Nonactivated astrocytes downregulate T cell receptor expression and reduce antigen-specific proliferation and cytokine production of myelin basic protein (MBP)-reactive T cells显示文摘 | Sun D Coleclough C Whitaker J N | 1997 | J Neuroimmunol1997，78，12： | 1 |
| 9 | Intercellular adhesion molecule expression in the evolving human cutaneous delayed hypersensitivity reaction 显示文摘 | Lewis RE Buchsbaum M Whitaker D | 1989 | J Invest Dermatol1989，93，5： | 1 |
| 10 | Attenuation of Platelet reactivity by enoxaparin compared with unfractionated heparin in patients undergoing haemodialysis 显示文摘 | Aggarwal A Whitaker D A Rimmer J M | 2004 | Nephrol Dial Transplant2004，19，6： | 1 |
| 11 | Relationship between white cell count, neuropsychologic outcome, and microemboli in 161 patients undergoing coronary artery bypass surgery 显示文摘 | Whitaker D Stygall J Harrison M | 2006 | J Thorac Cardiovasc Surg2006，131，6： | 1 |
| 12 | Response of chickens to temperature and relative humidity environment显示文摘 | PRINCE R P WHITAKER J H MATTERSON L D | 1965 | Poultry Science1965，44，1： | 1 |
| 13 | Effects of N - acetyl - cys- teine and N -acetyl -cysteine -amide Supplementation on In Vitro Matured porcine Oocytes 显示文摘 | Whitaker B D Knight J W | 2009 | Reproduc- tion in Domestic Animals2009，，： | 1 |
| 14 | jnter-cellular adhesion molecule expression in theevolving human cutaneous delayed liypersen-sitivity reaction 显示文摘 | Lewis RE Buchsbaum M Whitaker D | 1989 | Invest Dermatol1989，93，5： | 1 |
| 15 | Phytosterols,phytostanols, and their conjugates in foods: structural diversity,quantitative analysis, and health-promoting uses 显示文摘 | Moreau R A Whitaker B D Hicks K B | 2002 | Progress inLipid Research2002，41，6： | 1 |
| 16 | The rheumatoid arthritis risk gene LBH regulates growth in fibroblast-like synovi- ocytes显示文摘 | Ekwall AK Whitaker JW Hammaker D | 2015 | Arthritis Rheumatol2015，67，5： | 1 |
| 17 | N - acetyl - 1 - cysteine Supplementation Improves Boar Spermatozoa Characteristics and Subsequent Fertilization and Embry- onic Development 显示文摘 | Whitaker B D Casey S J Taupier R | 2012 | Reproduction in Domestic An- imals2012，47，2： | 1 |
| 18 | Control strategies for aeroallergens in an animal facility显示文摘 | Whitaker C K R Harrison D J Jones R B | 1999 | J Allergy Clin Immunol1999，103，： | 1 |
| 19 | A Level Set Approach for the Metamorphosis of Solid Models显示文摘 | Breen D Whitaker R T | 2001 | IEEE Transactions on Visualization and Computer Graphics2001，7，2： | 1 |
| 20 | Real-time reverse transcrip- tiowPCR assay panel for Middle East respiratory syn- drome corormvirus显示文摘 | Lu X Whitaker B Sakthivel S K Kamili S Rose L E Lowe L Mohareb E Elassal E M Al-sanouri T Haddadin A Erdman D D | 2014 | J Clin Microbiol2014，52，1： | 1 |
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