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| 1 | Dietary supplementation with Clostridium butyricum improves growth performance of broilers by regulating intestinal microbiota and mucosal epithelial cells显示文摘Clostridium butyricum has been widely considered an antibiotic substitute in recent years.It can promote growth performance,improve the immune response and enhance the intestinal barrier function of the host.In the present study,1-d-old Arbor Acres(AA)broilers were fed C.butyricum(1×109 cfu/kg)for 28 d.The transcriptomic characteristics of epithelial cells of the cecal mucosa were determined by RNA-sequence,and the cecal microbiota composition was explored by 16 S ribosomal RNA gene sequencing.The changes in the intestinal mucosa of broilers were then analyzed by tissue staining.Gene Ontology(GO)annotations identified substance transport and processes and pathways that might participate in intestinal development and cell viability.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis revealed that the differentially expressed genes are involved in numerous pathways related to amino acid and vitamin metabolism and antioxidant and defensive functions,among others.The relative expression of some genes associated with intestinal barrier function(claudins 2,15,19,and 23,tight junction proteins 1,2,and 3 and mucin 1)was significantly increased in the treatment group(P<0.05 or P<0.01).Moreover,the proportion of Firmicutes was higher in the C.butyricum-treated group,whereas the proportion of Proteobacteria was higher in the control group.At the genus level,the relative abundances of Butyricicoccus and Lactobacillus,among other bacteria,were increased after C.butyricum supplementation.The tissue staining analysis showed that the cecal mucosa of broilers was significantly ameliorated after the addition of C.butyricum(P<0.05 or P<0.01).These results showed that dietary supplementation with C.butyricum can enhance the antioxidant capacity,mucosal barrier function,and stabilize the cecal microbiota,resulting in improving the growth performance. | Laipeng Xu Xiangli Sun Xianhua Wan Keke Li Fuchun Jian Wenting Li Ruirui Jiang Ruili Han Hong Li Xiangtao Kang Yanbin Wang | 2021 | Animal Nutrition2021，，4： | 12 |
| 2 | Possible mechanisms underlying treatment of Alzheimer’s disease with Traditional Chinese Medicine: active components, potential targets and synthetic pathways of Bulao Elixir显示文摘OBJECTIVE: To elucidate the mechanisms underlying the treatment of Alzheimer’s disease(AD) with Traditional Chinese Medicine(TCM), by examining the active components, potential targets and synthetic pathways of Bulao Elixir(BLE).METHODS: The Absorption, Distribution, Metabolism, Excretion(ADME)/Toxicology(T) calculation was used to screen the active components of Bulao Elixir. Based on the TCM Systems Pharmacology Analysis Platform(TCMSP database) and a text mining tool(GoPubMed database), we predicted and screened the active components of Bulao Elixir and its therapeutic targets for AD. Using the Database for Annotation, Visualization and Integrated Discovery(DAVID), we obtained the targets for AD. Cytoscape software was used to establish a network map of the active component-target and target-pathway of Bulao Elixir. Gene function, related biological processes and signaling pathways were analyzed using the DAVID database.RESULTS: Twelve active components were selected from 196 components of Bulao Elixir. Among 2209 targets, 102 effective targets were selected, and 30 important targets were identified via matching with the disease targets. After further analysis, 14 core targets were identified. Enrichment analysis revealed that most of these important targets were involved in multiple biological processes, including apoptosis, inflammatory reactions, and cell regulation cycles. The synthetic pathways for AD treatment were identified after analyzing and confirming the relevant pathways, providing potentially useful information for diagnosis and treatment methods for AD.CONCLUSION: The current study elucidated the potential treatment mechanisms of Bulao Elixir in AD using network pharmacology, providing a foundation for further clarification of its treatment targets. | Ren Beida Cheng Fafeng Wang Xueqian Wan Yuxiang Ji Wenting Du Xin Zhang Shuang Liu Shilling Ma Chongyang Xiong Yiliang Hao Gaoting Wang Qingguo | 2020 | Journal of Traditional Chinese Medicine2020，40，3： | 4 |
| 3 | Identification of Key Genes for the Ultrahigh Yield of Rice Using Dynamic Cross-tissue Network Analysis显示文摘Significantly increasing crop yield is a major and worldwide challenge for food supply and security.It is well-known that rice cultivated at Taoyuan in Yunnan of China can produce the highest yield worldwide.Yet,the gene regulatory mechanism underpinning this ultrahigh yield has been a mystery.Here,we systematically collected the transcriptome data for seven key tissues at different developmental stages using rice cultivated both at Taoyuan as the case group and at another regular rice planting place Jinghong as the control group.We identified the top 24 candidate high-yield genes with their network modules from these well-designed datasets by developing a novel computational systems biology method,i.e.,dynamic cross-tissue(DCT)network analysis.We used one of the candidate genes,Os SPL4,whose function was previously unknown,for gene editing experimental validation of the high yield,and confirmed that Os SPL4 significantly affects panicle branching and increases the rice yield.This study,which included extensive field phenotyping,cross-tissue systems biology analyses,and functional validation,uncovered the key genes and gene regulatory networks underpinning the ultrahigh yield of rice.The DCT method could be applied to other plant or animal systems if different phenotypes under various environments with the common genome sequences of the examined sample.DCT can be downloaded from http://gffzz188fe103f8f1460as55kku6nbbbo96qu9.ffgz.tsg.suse.edu.cn/ztpub/DCT. | Jihong Hu Tao Zeng Qiongmei Xia Liyu Huang Yesheng Zhang Chuanchao Zhang Yan Zeng Hui Liu Shilai Zhang Guangfu Huang Wenting Wan Yi Ding Fengyi Hu Congdang Yang Luonan Chen Wen Wang | 2020 | Genomics, Proteomics & Bioinformatics2020，18，3： | 2 |
| 4 | Genome and Comparative Transcriptomics of African Wild Rice Oryza Iongistaminata Provide Insights into Molecular Mechanism of Rhizomatousness and Self-Incompatibility显示文摘Dear Editor, Oryza Iongistaminata is an African wild rice species with AA genome type possessing special traits that are highly valued for improving cultivated rice,such as strong resistance to biotic and abiotic stresses (Song et al.,1995) for improving resistance of cultivars,rhizomatousness for perennial breeding (Glover et al.,2010),and self-incompatibility (SI) for new ways to produce hybrid seeds (Ghesquiere,1986).Deciphering the genome of O.longistaminata will be the key to uncovering the mechanism of these hallmark traits and improving cultivated rice. | Yesheng Zhang Shilai Zhang Hui Liu Binying Fu Lijuan Li Min Xie Yue Song Xin Li Jing Cai Wenting Wan Ling Kui Hui Huang Jun Lyu Yang Dong Wensheng Wang Liyu Fluang Jing Zhang Qinzhong Yang Qinfi Shan Qiong Li Wangqi Huang Dayun Tao Muhua Wang Mingsheng Chen Yeisoo Yu Rod A. Wing Wen Wang Fengyi Hu | 2015 | Molecular Plant2015，8，11： | 1 |
| 5 | Multi-objective and multi-scheme research on water and sediment regulation potential of reservoirs in the upper Yellow River显示文摘The problems of the deteriorating relation between water and sediment,and the escalating conflict between water supply and demand in the upper Yellow River(YR),need to be addressed.Reservoirs of Longyangxia,Liujiaxia,and Heishanxia(Long-Liu-Hei)in the upper YR are taken as the research object,and a multi-objective,water-sediment optimal operation model for cascade reservoirs Long-Liu-Hei has been developed.This operation model considers the comprehensive requirements of water supply,power generation,flood control,and especially,water and sediment regulation.The improved Non-dominated Sorting Genetic Algorithms(NSGA-II)method is used to demonstrate the water and sediment regulation potential of three scenario schemes under long-term conditions,and the adjustable water allocation of four schemes under typical year conditions are calculated.It is recommended to use the mode of generating hydropower depending on the available water amount as the best operating mode under the conditions of a long time series and for typical years.This recommended operation mode has verified the rationality and accuracy of the reservoir operations model.At the same time,compared with the calculation results for water and sediment regulation after the construction of the Heishanxia,the current research indicates:(1)Under the recommended model,the potential for water and sediment regulation under the long-term series and typical years is once every four years.(2)Inter-basin water transfer greatly improves the cascade power generation.When the adjustable water volume is converted into the controlled volume for water and sediment regulation,the intensity frequency of water and sediment regulation of cascade reservoirs are improved.(3)With the intensification of the contradiction between the supply of and demand for water resources in the upper YR in the future,the amount of transferable water exhibits a decreasing trend,and the regulation of water and sediment in the upper YR is facing huge challenges.(4)Through joint operation with the Longyangxia and Liujiaxia reservoirs(Long-Liu),Heishanxia will play an important role in the regulation and control of power generation,water supply,flood control,and ice prevention in the upper YR.The current research results can provide a theoretical basis and technical support for water and sediment regulation in the YR basin. | Tao Bai Jia Yu Wenting Jin Jiaquan Wan Shaojie Gou Xu Ma Panpan Ma | 2023 | International Journal of Sediment Research2023，38，2： | 1 |
| 6 | Functionalization of in vivo tissue-engineered living biotubes enhance patency and endothelization without the requirement of systemic anticoagulant administration显示文摘Vascular regeneration and patency maintenance,without anticoagulant administration,represent key developmental trends to enhance small-diameter vascular grafts(SDVG)performance.In vivo engineered autologous biotubes have emerged as SDVG candidates with pro-regenerative properties.However,mechanical failure coupled with thrombus formation hinder translational prospects of biotubes as SDVGs.Previously fabricated poly(ε-caprolactone)skeleton-reinforced biotubes(PBs)circumvented mechanical issues and achieved vascular regeneration,but orally administered anticoagulants were required.Here,highly efficient and biocompatible functional modifications were introduced to living cells on PB lumens.The 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-methoxy(DMPE)-PEG-conjugated anti-coagulant bivalirudin(DPB)and DMPE-PEG-conjugated endothelial progenitor cell(EPC)-binding TPS-peptide(DPT)modifications possessed functionality conducive to promoting vascular graft patency.Co-modification of DPB and DPT swiftly attained luminal saturation without influencing cell viability.DPB repellent of non-specific proteins,DPB inhibition of thrombus formation,and DPB protection against functional masking of DPT’s EPC-capture by blood components,which promoted patency and rapid endothelialization in rat and canine artery implantation models without anticoagulant administration.This strategy offers a safe,facile,and fast technical approach to convey additional functionalization to living cells within tissue-engineered constructs. | Hongyu Yan Quhan Cheng Jianghua Si Songdi Wang Ye Wan Xin Kong Ting Wang Wenting Zheng Muhammad Rafique Xiaofeng Li Ju He Adam C.Midgley Yi Zhu Kai Wang | 2023 | Bioactive Materials2023，，8： | 0 |
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