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| 1 | Rhizobacterium Bacillus amyloliquefaciens Strain SQRT3-Mediated Induced Systemic Resistance Controls Bacterial Wilt of Tomato显示文摘Tomato bacterial wilt caused by Ralstonia solanacearum seriously threats tomato growth in tropical and temperate regions around the world.This study reported an antagonistic bacterial strain, Bacillus amyloliquefaciens strain SQRT3, isolated from the rhizosphere soil of tomato plants, which strongly inhibited in vitro growth of pathogenic R.solanacearum.The suppression of tomato bacterial wilt by strain SQRT3 was demonstrated under greenhouse conditions.Additionally, induced systemic resistance(ISR) in tomato as one of the potential disease suppression mechanisms was investigated in the plants inoculated with the isolated bacterial strain SQRT3.The results showed that strain SQRT3 applied with R.solanacearum by drenching significantly reduced tomato bacterial wilt by 68.1% biocontrol efficiency(BE) and suppressed the R.solanacearum populations in the rhizosphere soil compared to the control only drenched with R.solanacearum.The BE of the isolated bacterial strain SQRT3 against tomato wilt increased to 84.1%by root-dipping.Tomato plants treated with both strain SQRT3 and R.solanacearum showed increases in activities of peroxidase and polyphenol oxidase compared with other treatments.The application of strain SQRT3 reduced membrane lipid peroxidation in tomato leaves.The expressions of marker genes for jasmonic acid-and salicylic acid-dependent signaling pathways were faster and stronger in tomato plants treated with both strain SQRT3 and R.solanacearum than in plants treated with either R.solanacearum or strain SQRT3 alone.Collectively, the findings indicated that strain SQRT3 can effectively control tomato wilt. | LI Chunyu HU Weicong PAN Bin LIU Yan YUAN Saifei DING Yuanyuan LI Rong ZHENG Xinyan SHEN Biao SHEN Qirong | 2017 | Pedosphere2017，27，6： | 18 |
| 2 | Association between interferon gamma receptor 1-56C/T gene polymorphism and tuberculosis susceptibility: a meta-analysis显示文摘 | Wang Wei Ren Weicong Zhang Xuxia Liu Yi Li Chuanyou | 2014 | Chinese Medical Journal2014，，21： | 3 |
| 3 | Amorphization activated FeB_(2) porous nanosheets enable efficient electrocatalytic N_(2) fixation显示文摘Designing active,robust and cost-effective catalysts for the nitrogen reduction reaction(NRR) is of paramount significance for sustainable electrochemical NH3 synthesis.Transition-metal diborides(TMB_2)have been recently theoretically predicted to be a new class of potential NRR catalysts,but direct experimental evidence is still lacking.Herein,we present the first experimental demonstration that amorphous FeB_2 porous nanosheets(a-FeB_2 PNSs) could be a highly efficient NRR catalyst,which exhibited an NH3 yield of 39.8 μg h^(-1) mg^(-1)(-0.3 V) and a Faradaic efficiency of 16.7%(-0.2 V),significantly outperforming their crystalline counterpart and most of existing NRR catalysts.First-principle calculations unveiled that the amorphization could induce the upraised d-band center of a-FeB_2 to boost d-2π~* coupling between the active Fe site and ~*N_2 H intermediate,resulting in enhanced ~*N_2 H stabilization and reduced reaction barrier.Out study may facilitate the development and understanding of earth-abundant TMB_2-based catalysts for electrocatalytic N_2 fixation. | Ke Chu Weicong Gu Qingqing Li Yaping Liu Ye Tian Wuming Liu | 2021 | Journal of Energy Chemistry2021，30，2： | 2 |
| 4 | A cost-effective detection of low-abundance mutation with DNA three-way junction structure and lambda exonuclease显示文摘We presented a low-abundance mutation detection method with lambda exonuclease and DNA threeway junction structure.The assistant strand in the DNA three-way junction structure could regulate the reaction system from the kinetics and thermodyna mics aspects.The optimization of the assista nt strand helps to improve the selectivity of the mutant-type DNA to the wild-type DNA about 35 times.Moreover,the cost of the optimization process could be saved by about 90%.The method was applied to the detection of a human ovarian cancer-related gene mutation BRCA1(rs1799949,c.2082 C>T).The limit of detection to the mutation abundance in the DNA three-way junction structure system(0.2%) was one order lower compared with that in the double-stranded DNA structure system(2%).The mutation abundance in different standard samples was quantitively measured,and the results were consistent with the initial abundance in the standard samples. | Zishan Feng Wei Zhang Longjie Li Bocheng Tu Weicong Ye Xiaofeng Tang Hongbo Wang Xianjin Xiao Tongbo Wu | 2021 | Chinese Chemical Letters2021，32，2： | 1 |
| 5 | Method for measuring the steering wheel angle of paddy field agricultural machinery by integrating RTK-GNSS and dual-MEMS gyroscope显示文摘Aiming at the application environment of paddy agricultural machinery with bumpy and undulating changes,the problems affecting the method for steering wheel angle measurement by MEMS gyroscope were analyzed,and a wheel angle measurement method combining Dual-MEMS gyroscope(dual MEMS gyroscope)and RTK-GNSS was designed.The adaptive weighting method was used to fuse the heading angle differentiation of RTK-GNSS,the MEMS gyroscope angle rate,and velocity data,and the rod-arm compensation was performed to accurately obtain the angle rates of the body and steering wheels of agricultural machinery;the difference between the combined angular rate of the steering wheel of the agricultural machinery and the angular rate of the agricultural machinery body was obtained,and the integrator is used to integrate the difference to get the wheel steering angle value,and the Kalman filter was designed to make feedback correction for the integration process of angle calculation to eliminate the errors caused by the gyroscope zero bias,random drift,and gyroscope rod arm effect,and to obtain the accurate value of wheel steering angle.A comparative test with the connecting rod wheel angle sensor was designed,and the results show that the maximum deviation is 4.99°,the average absolute average value is 1.61°,and the average standard deviation is 0.98°.The method in this study and the connecting rod wheel angle sensor were used on paddy farm machinery.The wheel angle measurement deviation of the proposed method and the connecting rod wheel angle sensor was not more than 1°,which is relatively small.It has good stability,speed adaptability,and dynamic responsiveness that meets the accuracy requirements of steering wheel angle measurement for paddy field agricultural machinery unmanned driving and can be used instead of connecting rod angle sensors for unmanned agricultural machinery. | Pei Wang Lian Hu Jie He Siqi Ke Zhongxian Man Tuanpeng Tu Luning Yang Yuanyuan Li Yanling Yi Weicong Li Xiwen Luo | 2022 | International Journal of Agricultural and Biological Engineering2022，15，6： | 1 |
| 6 | The information rate of secret sharing schemes based on seven participants by connect graphs显示文摘 | SONG Yun LI Zhihui WANG Weicong | 2012 | Recent Advance in Computer Science and Information Engineering Lecture Notes in Electrical Engineering2012，127，： | 1 |
| 7 | The OX40-TRAF6 axis promotes CTLA-4 degradation to augment antitumor CD8^(+)T-cell immunity显示文摘Immune checkpoint blockade(ICB),including anti-cytotoxic T-lymphocyte associated protein 4(CTLA-4),benefits only a limited number of patients with cancer.Understanding the in-depth regulatory mechanism of CTLA-4 protein stability and its functional significance may help identify ICB resistance mechanisms and assist in the development of novel immunotherapeutic modalities to improve ICB efficacy.Here,we identified that TNF receptor-associated factor 6(TRAF6)mediates Lys63-linked ubiquitination and subsequent lysosomal degradation of CTLA-4.Moreover,by using TRAF6-deficient mice and retroviral overexpression experiments,we demonstrated that TRAF6 promotes CTLA-4 degradation in a T-cell-intrinsic manner,which is dependent on the RING domain of TRAF6.This intrinsic regulatory mechanism contributes to CD8+T-cell-mediated antitumor immunity in vivo.Additionally,by using an OX40 agonist,we demonstrated that the OX40-TRAF6 axis is responsible for CTLA-4 degradation,thereby controlling antitumor immunity in both tumor-bearing mice and patients with cancer.Overall,our findings demonstrate that the OX40-TRAF6 axis promotes CTLA-4 degradation and is a potential therapeutic target for the improvement of T-cell-based immunotherapies. | Jizhang Yu Jikai Cui Xi Zhang Heng Xu Zhang Chen Yuan Li Yuqing Niu Song Wang Shuan Ran Yanqiang Zou Weicong Ye Dan Zhang Cheng Zhou Jiahong Xia Jie Wu | 2023 | Cellular & Molecular Immunology2023，20，12： | 0 |
| 8 | Role of artificial intelligence in early detection and screening for pancreatic adenocarcinoma显示文摘Pancreatic adenocarcinoma remains to be one of the deadliest malignancies in the world despite treatment advancement over the past few decades.Its low survival rates and poor prognosis can be attributed to ambiguity in recommendations for screening and late symptom onset,contributing to its late presentation.In the recent years,artificial intelligence(AI)as emerged as a field to aid in the process of clinical decision making.Considerable efforts have been made in the realm of AI to screen for and predict future development of pancreatic ductal adenocarcinoma.This review discusses the use of AI in early detection and screening for pancreatic adenocarcinoma,and factors which may limit its use in a clinical setting. | Kenneth Weicong Lin Tiing Leong Ang James Weiquan Li | 2022 | Artificial Intelligence in Medical Imaging2022，3，2： | 0 |
| 9 | Ka-band broadband filtering packaging antenna based on through-glass vias (TGVs)显示文摘This work presents a novel design of Ka-band(33 GHz)filtering packaging antenna(FPA)that features broadband and great filtering response,and is based on glass packaging material and through-glass via(TGV)technologies.Compared to traditional packaging materials(printed circuit board,low temperature co-fired ceramic,Si,etc.),TGVs are more suitable for miniaturization(millimeter-wave three-dimensional(3D)packaging devices)and have superior microwave performance.Glass substrate can realize 3D high-density interconnection through bonding technology,while the coefficient of thermal expansion(CTE)matches that of silicon.Furthermore,the stacking of glass substrate enables high-density interconnections and is compatible with micro-electro-mechanical system technology.The proposed antenna radiation patch is composed of a patch antenna and a bandpass filter(BPF)whose reflection coefficients are almost complementary.The BPF unit has three pairs ofλg/4 slots(defect microstrip structure,DMS)and twoλg/2 U-shaped slots(defect ground structure,DGS).The proposed antenna achieves large bandwidth and high radiation efficiency,which may be related to the stacking of glass substrate and TGV feed.In addition,the introduction of four radiation nulls can effectively improve the suppression level in the stopband.To demonstrate the performance of the proposed design,a 33-GHz broadband filtering antenna is optimized,debugged,and measured.The antenna could achieve|S11|<-10 dB in 29.4‒36.4 GHz,and yield an impedance matching bandwidth up to 21.2%,with the stopband suppression level at higher than 16.5 dB.The measurement results of the proposed antenna are a realized gain of~6.5 dBi and radiation efficiency of~89%. | Zhen FANG Jihua ZHANG Libin GAO Hongwei CHEN Wenlei LI Tianpeng LIANG Xudong CAI Xingzhou CAI Weicong JIA Huan GUO Yong LI | 2023 | Frontiers of Information Technology & Electronic Engineering2023，24，6： | 0 |
| 10 | Overview on artificial intelligence in design of Organic Rankine Cycle显示文摘Converting thermal energy into mechanical work by means of Organic Rankine Cycle is a validated technology to exploit low-grade waste heat.The typical design process of Organic Rankine Cycle system,which commonly in-volves working fluid selection,cycle configuration selection,operating parameters optimization,and component selection and sizing,is time-consuming and highly dependent on engineer’s experience.Thus,it is difficult to achieve the optimal design in most cases.In recent decades,artificial intelligence has been gradually introduced into the design of energy system to overcome above shortcomings.In order to clarify the research field of arti-ficial intelligence technique in Organic Rankine Cycle design and guide artificial intelligence technique to assist Organic Rankine Cycle design better,this study presents a preliminary literature summary on recent progresses of artificial intelligence technique in organic Rankine cycle systems design.First,this study analyzes four main procedures which constitute a typical design process of Organic Rankine Cycle systems and finds that design problems encountered during design process can be divided into three categories:decision making,parameter optimization and parameter prediction.In the second section,a detailed literature review on each design proce-dures using artificial intelligence algorithms is presented.At last,the state of art in this field and the prospects for the future work are provided. | Dongpeng Zhao Shuai Deng Li Zhao Weicong Xu Wei Wang Xianhua Nie Mengchao Chen | 2020 | Energy and AI2020，1，1： | 0 |
| 11 | Transformation of coccolithophorid Emiliania huxleyi harboring a marine virus(Coccolithoviruses)serine palmitoyltransferase(SPT)gene by electroporation显示文摘Emiliania huxleyi is the most prominent modern coccolithophore,a group of marine unicellular eukaryotes that play a critical role in ocean biogeochemistry.Coccolithoviruses are large double stranded DNA viruses,which is responsible for the demise of large oceanic blooms formed by E.huxleyi.E.huxleyi virus(EhVs)acquired a series of enzyme-coding genes predicted to be involved in the sphingolipid biosynthesis by horizontal gene transfer between virus-host.Currently,there is limited experimental validation identifying the functions of these genes in EhV.Genetic transformation of eukaryotic cells is a powerful tool to get an insight into gene functions of the studied organisms.Serine palmitoyltransferase(SPT)catalyzes the first committed step in de novo sphingolipid biosynthetic pathway.Here,a novel vector system for the transformation of E.huxleyi was designed.It contained fragments of promoter and terminator sequences of E.huxleyi endogenic fucoxanthin chlorophyll a/c-binding protein gene“fcp”and harbored EhV-99B1 spt gene.The resultant recombinant transformation vectors pEhux-I-spt and pEhux-II were co-transferred into E.huxleyi BOF92 by electroporation.Transformants were obtained upon glufosinate-ammonium selection,and confirmed by Southern hybridization,genome PCR,qRT-PCR and Western blot screening of spt gene,which indicated that spt gene was integrated into the nuclear genome and was expressed at the mRNA and protein levels.The expression of the viral spt gene led to differences in lipid compositions analyzed using thin-layer chromatography(TLC).The results present the genetic transformation system for E.huxleyi,providing additional genetic resource with potential for exploring basic biological questions such as the virus-host interactions. | Weicong CAI Xueting WANG Jinjing SU Jian LI Jun ZENG Guiling LI Jingwen LIU | 2021 | Journal of Oceanology and Limnology2021，39，2： | 0 |
| 12 | Sensitive detection of alkaline phosphatase based on terminal deoxynucleotidyl transferase and endonuclease Ⅳ-assisted exponential signal amplification显示文摘Alkaline phosphatase(ALP)is widely expressed in human tissues.ALP plays an important role in the dephosphorylation of proteins and nucleic acids.Therefore,quantitative analysis of ALP plays a vital role in disease diagnosis and the development of biological detection methods.Terminal deoxynucleotidyl transferase(TdT)catalyzes continuous polymerization of deoxynucleotide triphosphates at the 30-OH end of single-stranded DNA in the absence of a template.In this study,we developed a highly sensitive and selective method based on TdT and endonuclease Ⅳ(Endo Ⅳ)to quantify ALP activity.After ALP hydrolyzes the 30-PO_(4) end of the substrate and generates 30-OH,TdT can effectively elongate the 30-OH end with deoxynucleotide adenine triphosphate(dATP)and produce a poly A tail,which can be detected by the poly T probes.Endo Ⅳ digests the AP site in poly T probes to generate a fluorescent signal and a new 30-OH end,leading to the generation of exponential fluorescence signal amplification.The substrate for TdT elongation was optimized,and a limit of detection of 4.3×10^(-3) U/L was achieved for ALP by the optimized substrate structure.This method can also detect ALP in the cell lysate of a single cell.This work has potential applications in disease diagnosis and biomedical detection. | Weicong Ye Longjie Li Zishan Feng Bocheng Tu Zhe Hu Xianjin Xiao Tongbo Wu | 2022 | Journal of Pharmaceutical Analysis2022，12，4： | 0 |
| 13 | On m-ovoids of finite classical polar spaces with an irreducible transitive automorphism group显示文摘In this paper, we classify the m-ovoids of finite classical polar spaces that admit a transitive automorphism group acting irreducibly on the ambient vector space. In particular, we obtain several new infinite families of transitive m-ovoids. | Tao Feng Weicong Li Ran Tao | 2024 | Science China Mathematics2024，67，3： | 0 |
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