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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | TALEN-based generation of a cynomolgus monkey disease model for human microcephaly显示文摘基因在非人类的首领编辑可以为在人探索病原学和遗传上基于的神经病学的混乱的治疗学的策略导致珍贵模型。然而,仔细在人模仿病理学、行为的赤字的神经病学的混乱的一个猴子模型成功地还没被产生了。Microcephalin 1 (MCPH1 ) 在人的大脑,和智力迟钝伴随的 MCPH1 变化原因头小畸型的进化被含有。这里,我们产生了用抄写带 biallelic MCPH1 变化的一只 cynomolgus 猴子(Macaca fascicularis ) 像使活跃之物的受动器核酸酶。猴子概括了大多数在病人观察的重要临床的特征,包括在头圆周,早熟的染色体冷凝作用(PCC ) ,语料库 callosum 的发育不全和上面的手足 spasticity 的显著减小。而且,在变异的真皮的成纤维细胞的 MCPH1 的 overexpression 救了 PCC 症候群。这个猴子模型可以帮助我们阐明在人的头小畸型并且更好的致病的 MCPH1 的角色在首领大脑尺寸的进化理解这蛋白质的功能。 | Qiong Ke Weiqiang Li Xingqiang Lai Hong Chen Lihua Huang Zhuang Kang Kai Li Jie Ren Xiaofeng Lin Haiqing Zheng Weijun Huang Yunhan Ma Dongdong Xu Zheng Chen Xinming Song Xinyi Lin Min Zhuang Tao Wang Fengfeng Zhuang Jianzhong Xi Frank Fuxiang Mao Huimin Xia Bruce T Lahn Qi Zhou Shihua Yang Andy Peng Xiang | 2016 | Cell Research2016，26，9： | 13 |
| 3 | Endothelial CDS2 deficiency causes VEGFA-mediated vascular regression and tumor inhibition显示文摘The response of endothelial cells to signaling stimulation is critical for vascular morphogenesis,homeostasis and function.Vascular endothelial growth factor-a(VEGFA)has been commonly recognized as a pro-angiogenic factor in vertebrate developmental,physiological and pathological conditions for decades.Here we report a novel finding that genetic ablation of CDP-diacylglycerol synthetase-2(CDS2),a metabolic enzyme that controls phosphoinositide recycling,switches the output of VEGFA signaling from promoting angiogenesis to unexpectedly inducing vessel regression.Live imaging analysis uncovered the presence of reverse migration of the angiogenic endothelium in cds2 mutant zebrafish upon VEGFA stimulation,and endothelium regression also occurred in postnatal retina and implanted tumor models in mice.In tumor models,CDS2 deficiency enhanced the level of tumorsecreted VEGFA,which in-turn trapped tumors into a VEGFA-induced vessel regression situation,leading to suppression of tumor growth.Mechanistically,VEGFA stimulation reduced phosphatidylinositol(4,5)-bisphosphate(PIP2)availability in the absence of CDS2-controlled-phosphoinositide metabolism,subsequently causing phosphatidylinositol(3,4,5)-triphosphate(PIP3)deficiency and F0X01 activation to trigger regression of CDS2-null endothelium.Thus,our data indicate that the effect of VEGFA on vasculature is context-dependent and can be converted from angiogenesis to vascular regression. | Wencao Zhao Le Cao Hanru Ying Wenjuan Zhang Dantong Li Xiaolong Ziiu Wenzhi Xue Shuang Wu Mengye Cao Cong Fu Haonan Qi Yimei Hao Yun-Chi Tang Jun Qin Tao PZhong Xiaoxi Lin Luyang Yu Xuri Li Lin Li Dianqing Wu Weijun Pan | 2019 | Cell Research2019，29，11： | 9 |
| 4 | Acrylamide exposure impairs blood-cerebrospinal fluid barrier function显示文摘Previous studies show that chronic acrylamide exposure leads to central and peripheral neuropathy. However, the underlying mechanisms remained unclear. In this study, we examined the permeability of the blood-cerebrospinal fluid barrier, and its ability to secrete transthyretin and transport leptin of rats exposed to acrylamide for 7, 14, 21 or 28 days. Transthyretin levels in cerebrospinal fluid began to decline on day 7 after acrylamide exposure. The sodium fluorescein level in cerebrospinal fluid was increased on day 14 after exposure. Evans blue concentration in cerebrospinal fluid was increased and the cerebrospinal fluid/serum leptin ratio was decreased on days 21 and 28 after exposure. In comparison, the cerebrospinal fluid/serum albumin ratio was increased on day 28 after exposure. Our findings show that acrylamide exposure damages the blood-cerebrospinal fluid barrier and impairs secretory and transport functions. These changes may underlie acrylamide-induced neurotoxicity. | Xue Yao Licheng Yan Lin Yao Weijun Guan Fanxu Zeng Fuyuan Cao Yanshu Zhang | 2014 | Neural Regeneration Research2014，9，5： | 4 |
| 5 | Atmospheric fine particles in a typical coastal port of Yangtze River Delta显示文摘In recent decades, coastal ports have experienced rapid development and become an important economic and ecological hub in China. Atmospheric particle is a research hotspot in atmospheric environmental sciences in inland regions. However, few studies on the atmospheric particle were conducted in coastal port areas in China, which indeed suffers atmospheric particle pollution. Lack of the physicochemical characteristics of fine particles serves as an obstacle toward the accurate control for air pollution in the coastal port area in China. Here, a field observation was conducted in an important coastal port city in Yangtze River Delta from March 6 to March 19, 2019. The average PM2.5 concentration was 63.7 ±27.8 μg/m^3 and NO3^-, SO4^2-, NH4^+, and organic matter accounted for ?60% of PM 2.5. Fe was the most abundant trace metal element and V as the ship emission indicator was detected. Transmission electron microscopy images showed that SK-rich, soot, Fe, SK-soot and SK-Fe were the major individual particles in the coastal port. V and soluble Fe were detected in sulfate coating of SK-Fe particles. We found that anthropogenic emissions, marine sea salt, and secondary atmosphere process were the major sources of fine particles. Backward trajectory analysis indicated that the dominant air masses were marine air mass, inland air mass from northern Zhejiang and inland-marine mixed air mass from Shandong and Shanghai during the sampling period. The findings can help us better understand the physicochemical properties of atmospheric fine particles in the coastal port of Eastern China. | Qi Yuan Xiaomi Teng Shaoxuan Tu Binxin Feng Zhiyu Wu Hang Xiao Qiuliang Cai Yinxiao Zhang Qiuhan Lin Zhaoce Liu Mengmeng He Xiaokun Ding Weijun Li | 2020 | Journal of Environmental Sciences2020，32，12： | 4 |
| 6 | The emission inventory of atmospheric industrial pollution sources of the five cities in Zhejiang Province显示文摘The amount of several air pollutants emitted in some cities including Hangzhou,Ningbo,Huzhou,Shaoxing and Jiaxing of Zhejiang Province is based on pollution source census data of Zhejiang Province in 2010.This paper focused on the release of air pollutants such as NO_(x2),SO_2,CO,PM2.5,PM10 and VOC,and calculated the total amount of those air pollutants.It analyzed air pollutant emission factors and found that the electricity and heat production industry released the largest amount of pollutants. | Jian Wu Chen Wen Lin Yi Weijun Wang | 2014 | Chinese Journal of Population,Resources and Environment2014，12，2： | 3 |
| 7 | An element by element spectral element method for elastic wave modeling显示文摘光谱联合优点的元素方法光谱有限元素方法的有那些的方法,在在复杂媒介模仿有弹性的波浪方程提供一个有效工具。基于从 elastodynamic 方程弱,为 Legendre 的数学明确的表达光谱元素方法被介绍。一个元素上的波浪地是用高顺序的 Lagrange 插值的 discretized,并且在元素上的集成被完成在 Gauss-Lobotto-Legendre 集成规则之上基于。这导致导致一个极大地简化的算法的一个斜集体矩阵。另外,由元素例子的元素被恨到在我们的方法到减少记忆尺寸并且改进计算效率。最后,一些数字例子被恨示威光谱精确性和效率。因为有限元素的联合,策划,包括免费表面边界和强壮的异质并且一光谱算法。 | LIN Weijun WANG Xiuming ZHANG Hailan | 2006 | Progress in Natural Science:Materials International2006，16，1： | 3 |
| 8 | A study of peroxyacetyl nitrate at a rural site in Beijing based on continuous observations from 2015 to 2019 and the WRFChem model显示文摘Peroxyacetyl nitrate(PA N)is one of the most important photochemical pollutants and has aroused much concern in China in recent decades.However,few studies described the long-term variations in PAN in China.In this study,we continuously monitored the PAN,03 and NOx concentrations at a regional background site near Beijing from August 2015 to February 2019.Based on the observed concentrations and climate data,w e analyzed the seasonal PAN variations.The results revealed that the monthly mean PAN concentration ranged from 0.33-2.41 ppb,with an average value of 0.94 ppb.The PAN concentration exhibited a distinct seasonal variation,with high values in spring and low values in winter.After analyzing the corresponding meteorological data,we found that stronger ultraviolet(UV)radiation,a relatively longer lifetime and a higher background PAN concentration contributed to the high PAN concentrations in spring.In addition,with the utilization of the WRF-Chem(Weather Research and Forecasting with Chemistry)model,the cause of the extremely high PAN concentration in spring 2018 wa s determined.The model results demonstrated that an anomalously low pressure and the southwesterly winds in northern China might be the main causes of the increased PAN concentration in Beijing and its surrounding area in spring 2018. | Yulu Qiu Zhiqiang Ma Weili Lin Weijun Quan Weiwei Pu Yingruo Li Liyan Zhou Qingfeng Shi | 2020 | Frontiers of Environmental Science & Engineering2020，14，4： | 3 |
| 9 | Integrative square-grid triboelectric nanogenerator as a vibrational energy harvester and impulsive force sensor显示文摘方形格子的摩擦电 nanogenerator (SG-TENG ) 为收获震动的精力并且察觉到冲动的力量被表明。每个广场三维(3D ) 打印了方形的格子充满铝(艾尔) 球。格子结构允许 SG-TENG 在宽广带宽上收获震动的精力并且在不同震动的角度操作。SG-TENG 的最惹人注目的特征是它被放大的能力并且综合。在在平行连接二 SG-TENGs 以后,开电路的电压和电线走火电流显著地在完整的震动的频率范围上被增加。与一个乒乓球球拍是综合的, SG-TENG 能从用球拍击一个砰恶臬球收获震动的精力,在由球拍的直接命中产生一般水准的地方,输出 10.9 | Chuan He Weijun Zhu Guang Qin Gu Tao Jiang Liang Xu Bao Dong Chen Chang Bao Han Dichen Li Zhong Lin Wang | 2018 | Nano Research2018，11，2： | 3 |
| 10 | An acoustic imaging system of migration technique used in borehole显示文摘以便检测 casing 地上凿穿的损坏,有一个二维的超声的数组的一个声学的成像方法被介绍。数组的每个元素独立地在超声的波浪下面射出,所有元素收到的回响被取样并且在地面表面上传给一台计算机,动态移植方法在此被用来形成一 2 或在地上凿穿的状况的 3-dimensional 图象。数字模拟和实验被进行与在数组使用的元素的一个小数字表明高成像精确性。自从在传统的分阶段执行的数组成像使用的延期电路,系统不在这个系统被需要,并且所有数据进程能在地上凿穿在一个扎根的系统,复杂性和系统的卷被完成可以显著地被简化,它对地上凿穿仪器批评。 | LIN Weijun WU Nan SUN Jian ZHANG Hailan | 2008 | Chinese Journal of Acoustics2008，27，3： | 3 |
| 11 | CO flux of soil respiration in natural recovering karst abandoned farmland in Southwest China显示文摘To estimate carbon sequestration potential in the karst area,soil respiration in a natural recovering karst abandoned farmland in Shawan,Puding,Guizhou,southwest China was continuously and automatically monitored for more than two years.The results show that the CO2flux of soil respiration(2.63±1.89 lmol m^-2s-^1)is higher in the karst area than in non-karst areas under similar conditions but that regional value(1.32 lmol m-2s-1)is lower because of larger rock fragment coverage(~50%).A the same time,the temperature sensitivity of soil respiration(Q10)in this study area is significantly higher than that of non-karst areas under similar conditions.Soil respiration has an obvious temporal variation,which is reflected in a significant exponential relationship between soil respiration and soil temperature,but the relationship between soil respiration and soil moisture is very complex.Especially soil respiration has an obvious spatial variation,which is likely affected by different diffusion or water-rock reaction processes. | Yanwei Wang Weijun Luo Guangneng Zeng Hanling Yang Meifang Wang Yina Lyu Anyun Cheng Lin Zhang Xianli Cai Jia Chen Shijie Wang | 2020 | Acta Geochimica2020，39，4： | 2 |
| 12 | Two Ultraviolet Radiation Datasets that Cover China显示文摘Ultraviolet(UV) radiation has significant effects on ecosystems, environments, and human health, as well as atmospheric processes and climate change. Two ultraviolet radiation datasets are described in this paper. One contains hourly observations of UV radiation measured at 40 Chinese Ecosystem Research Network stations from 2005 to 2015. CUV3 broadband radiometers were used to observe the UV radiation, with an accuracy of 5%, which meets the World Meteorology Organization's measurement standards. The extremum method was used to control the quality of the measured datasets. The other dataset contains daily cumulative UV radiation estimates that were calculated using an all-sky estimation model combined with a hybrid model. The reconstructed daily UV radiation data span from 1961 to 2014. The mean absolute bias error and root-mean-square error are smaller than 30% at most stations, and most of the mean bias error values are negative, which indicates underestimation of the UV radiation intensity. These datasets can improve our basic knowledge of the spatial and temporal variations in UV radiation. Additionally, these datasets can be used in studies of potential ozone formation and atmospheric oxidation, as well as simulations of ecological processes. | Hui LIU Bo HU Yuesi WANG Guangren LIU Liqin TANG Dongsheng JI Yongfei BAI Weikai BAO Xin CHEN Yunming CHEN Weixin DING Xiaozeng HAN Fei HE Hui HUANG Zhenying HUANG Xinrong LI Yan LI Wenzhao LIU Luxiang LIN Zhu OUYANG Boqiang QIN Weijun SHEN Yanjun SHEN Hongxin SU Changchun SONG Bo SUN Song SUN Anzhi WANG Genxu WANG Huimin WANG Silong WANG Youshao WANG Wenxue WEI Ping XIE Zongqiang XIE Xiaoyuan YAN Fanjiang ZENG Fawei ZHANG Yangjian ZHANG Yiping ZHANG Chengyi ZHAO Wenzhi ZHAO Xueyong ZHAO Guoyi ZHOU Bo ZHU | 2017 | Advances in Atmospheric Sciences2017，34，7： | 2 |
| 13 | A numerical investigation of the acoustic mode waves in a deviated borehole penetrating a transversely isotropic formation显示文摘A 2.5-dimensional method in frequency wave-number domain is developed to investigate the mode waves in a deviated borehole penetrating a transversely isotropic formation. The phase velocity dispersion characteristics of the fast and slow flexural mode waves excited by a dipole source are computed accurately at various deviation angles for both hard and soft formations. The sensitivities of the flexural mode waves to all elastic constants in a transversely isotropic formation are calculated. Numerical results show that, for a soft formation, the fast flexural mode wave is dominated by c66 at high deviation angles and low frequencies, while the slow flexural mode wave is dominated by c44 at the same conditions. Waveforms in time domain are also presented to support the conclusions. | LIU Le LIN WeiJun ZHANG HaiLan WANG XiuMing | 2015 | Science China(Physics,Mechanics & Astronomy)2015，58，8： | 2 |
| 14 | Fascia ligment of obital muscle slingA new ideal for lower eyelid blepharoplasty显示文摘 | Wang Wei Wang Weijun Lin Xiaoxi | 2003 | ANZ J Surg Journal of Surgery Vol 73 Supplement 22003，2，： | 1 |
| 15 | Evaluation of abundant hydrocarbon-generation depressions in the deepwater area of Qiongdongnan Basin,South China Sea显示文摘It has been confirmed that the key source rocks of Qiongdongnan Basin are associated with the Yacheng Formation, which was deposited in a transitional marine-continental environment. Because the distribution and evolution patterns of the source rocks in the major depressions are different, it is important to determine the most abundant hydrocarbon-generation depressions in terms of exploration effectiveness. Based on an analysis of organic matter characteristics of the source rocks, in combination with drilling data and seismic data, this paper establishes a model to evaluate the hydrocarbon-generation depressions in the deepwater area of Qiongdongnan Basin. First of all, by using the method of seismic-facies model analysis, the distribution of sedimentary facies was determined. Then, the sedimentary facies were correlated with the organic facies, and the distribution of organic facies was predicted. Meanwhile, the thickness of source rocks for all the depressions was calculated on the basis of a quantitative analysis of seismic velocity and lithology. The relationship between mudstone porosity and vitrinite reflectance(Ro) was used to predict the maturity of source rocks. Second, using the parameters such as thickness and maturity of source rocks, the quantity and intensity of gas generation for Yacheng and Lingshui Formations were calculated. Finally, in combination with the identified hydrocarbon resources, the quantity and intensity of gas generation were used as a guide to establish an evaluation standard for hydrocarbon-generation depressions, which was optimized for the main depressions in the Central Depression Belt. It is proposed that Lingshui, Ledong, Baodao and Changchang Depressions are the most abundant hydrocarbon depressions, whilst Songnan and Beijiao Depressions are rich hydrocarbon depressions. Such an evaluation procedure is beneficial to the next stage of exploration in the deep-water area of Qiongdongnan Basin. | LIU Zhen SUN Zhipeng WANG Zisong ZHANG Wei LI Tingan HE Weijun LI Fengxia CAO Shang LIU Jingjing LIN Lu | 2016 | Acta Oceanologica Sinica2016，35，2： | 1 |
| 16 | Three-dimensional finite-element analysis of craniomaxillary complex during maxillary protraction with bone anchorage versus conventional dental anchorage显示文摘 | Xiulin Yan Weijun He Tao Lin Jun Liu Xiaofeng Bai Guangqi Yan Li Lu | 2012 | American Journal of Orthodontics & Dentofacial Orthopedics2012，，： | 1 |
| 17 | Mechanobiologically optimized Ti-35Nb-2Ta-3Zr improves load transduction and enhances bone remodeling in tilted dental implant therapy显示文摘The tilted implant with immediate function is increasingly used in clinical dental therapy for edentulous and partially edentulous patients with excessive bone resorption and the anatomic limitations in the alveolar ridge.However,peri-implant cervical bone loss can be caused by the stress shielding effect.Herein,inspired by the concept of“materiobiology”,the mechanical characteristics of materials were considered along with bone biology for tilted implant design.In this study,a novel Ti-35Nb-2Ta-3Zr alloy(TNTZ)implant with low elastic modulus,high strength and favorable biocompatibility was developed.Then the human alveolar bone environment was mimicked in goat and finite element(FE)models to investigate the mechanical property and the related peri-implant bone remodeling of TNTZ compared to commonly used Ti-6Al-4V(TC4)in tilted implantation under loading condition.Next,a layer-by-layer quantitative correlation of the FE and X-ray Microscopy(XRM)analysis suggested that the TNTZ implant present better mechanobiological characteristics including improved load transduction and increased bone area in the tilted implantation model compared to TC4 implant,especially in the upper 1/3 region of peri-implant bone that is“lower stress”.Finally,combining the static and dynamic parameters of bone,it was further verified that TNTZ enhanced bone remodeling in“lower stress”upper 1/3 region.This study demonstrates that TNTZ is a mechanobiological optimized tilted implant material that enhances load transduction and bone remodeling. | Chuanyuan Mao Weijun Yu Min Jin Yingchen Wang Xiaoqing Shang Lu Lin Xiaoqin Zeng Liqiang Wang Eryi Lu | 2022 | Bioactive Materials2022，7，10： | 1 |
| 18 | Identification of weathered troughs in granites for tunneling at Daya Bay reactor-neutrino experiment site显示文摘Weathered troughs are frequently encountered in granites.They can cause problems to tunneling in the rocks and have to be properly addressed.The structures or spatial shapes of weathered troughs were seldom clarified in the past.In this paper,four weathered troughs are identified by means of geophysical exploration,core drilling,logging,and in-situ stress measurement at Daya Bay reactor-neutrino experiment site in Shenzhen,China.The weathered troughs are exposed on the ground or partially covered by grass and soils,which will threaten the safety of horizontal tunneling at a shallow depth.High electrical resistivity(HER) method is adopted for its feasibility and practicality,in combination with field geological observation,ultrasonic televiewer in boreholes and in-situ stress measurement.By comparing the HER values of completely decomposed to fresh rocks,it is indicated that the HER values of weathered troughs in natural state are 0.4-100 ·m,and the in-situ stress is abnormally lower than those at upper and lower layers.Field investigations show that the depths of the four weathered troughs are 30-182 m,with bottom elevation over 10 m.The volume of each weathered trough is mostly over 1×10 6 m 3 in inverted conic form.The weathered troughs often occur in various kinds of landforms,such as ridges,gullies or gently dipping dish-like depression areas.Faults and boundaries of different granitic plutons as well as joints govern the formation,locations and strikes of these troughs under development. | Yanjun Shang Yongyue Shi Weijun Jin Daming Lin Fengbo Wu Wei Zhang | 2012 | Journal of Rock Mechanics and Geotechnical Engineering2012，4，2： | 1 |
| 19 | Characterization of polyethylene glycol - modified proteins by semi - aqueous capillary electrophoresis显示文摘 | LIA Weijun ZHONG Yi LIN Bingcheng | 2001 | J Chroma A2001，905，： | 1 |
| 20 | Host range, growth property, and virulence of the smallpox vaccine:Vaccinia virus Tian Tan strain显示文摘 | Qing Fang Lin Yang Weijun Zhu | 2005 | Virology2005，335，： | 1 |
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