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| 1 | The complete reference genome for grapevine (Vitis vinifera L.) genetics and breeding显示文摘Grapevine is one of the most economically important crops worldwide.However,the previous versions of the grapevine reference genome tipically consist of thousands of fragments with missing centromeres and telomeres,limiting the accessibility of the repetitive sequences,the centromeric and telomeric regions,and the study of inheritance of important agronomic traits in these regions.Here,we assembled a telomere-to-telomere(T2T)gap-free reference genome for the cultivar PN40024 using PacBio HiFi long reads.The T2T reference genome(PN_T2T)is 69 Mb longer with 9018 more genes identified than the 12X.v0 version.We annotated 67%repetitive sequences,19 centromeres and 36 telomeres,and incorporated gene annotations of previous versions into the PN_T2T assembly.We detected a total of 377 gene clusters,which showed associations with complex traits,such as aroma and disease resistance.Even though PN40024 derives from nine generations of selfing,we still found nine genomic hotspots of heterozygous sites associated with biological processes,such as the oxidation–reduction process and protein phosphorylation.The fully annotated complete reference genome therefore constitutes an important resource for grapevine genetic studies and breeding programs. | Xiaoya Shi Shuo Cao Xu Wang Siyang Huang Yue Wang Zhongjie Liu Wenwen Liu Xiangpeng Leng Yanling Peng Nan Wang Yiwen Wang Zhiyao Ma Xiaodong Xu Fan Zhang Hui Xue Haixia Zhong Yi Wang Kekun Zhang Amandine Velt Komlan Avia Daniela Holtgräwe Jérôme Grimplet JoséTomás Matus Doreen Ware Xinyu Wu Haibo Wang Chonghuai Liu Yuling Fang Camille Rustenholz Zongming Cheng Hua Xiao Yongfeng Zhou | 2023 | Horticulture Research2023，10，5： | 3 |
| 2 | The MOS 36-Item Short-Form Health Survey (SF-36) : Ⅲ : Tests of data quality, scaling assumptions and reliability across diverse patient groups显示文摘 | Mcklorney C A Ware J E Lu J F R | 1994 | Med Care1994，32，1： | 1 |
| 3 | Maintenance respiration and stand development in a subalpine lodgepole pine forest显示文摘 | Ryan M G Waring R H | 1992 | Ecology1992，73，： | 1 |
| 4 | MODIS enhanced vegetation index predicts tree species richness across forested ecoregions in the contiguous USA显示文摘 | Waring R H Coops N C Fan W | 2006 | Remote Sensing of Environment2006，103，2： | 1 |
| 5 | Membrane thinning effect of the betasheet antimicrobial protegrin显示文摘 | Heller W T Waring A J Lehrer R I | 2000 | Biochemistry2000，39，： | 1 |
| 6 | Treatment of ARDS显示文摘 | Brower R G Ware L B Berthiaume Y | 2001 | Chest2001，120，： | 1 |
| 7 | Predicting fine root production and turnover by monitoring root starch and soil temperature显示文摘 | Marashall J D Waring R H | 1985 | Can J For Res1985，15，： | 1 |
| 8 | Flaking corn: processing mechanics, quality standards, and impacts on energy availability and performance of feedlot cat- tle显示文摘 | ZINN R A OWENS F N WARE R A | 2002 | Journal of Animal Science2002，80，5： | 1 |
| 9 | GPS sounding of the atmosphere from low Earth orbit: Preliminary results 显示文摘 | Ware R Exner M Feng D | 1996 | Bulletin of the American Meteo- rological Society1996，77，： | 1 |
| 10 | Stem Growth Per Unit of Leaf Area:a Measure of Tree Vigour显示文摘 | Waring R H Thies W G Muscato D | 1980 | Forest Sci1980，26，： | 1 |
| 11 | Comparison of methods of estimating leaf-area index in old-growth Douglas-fir显示文摘 | Marshall J D Waring R H | 1986 | Ecology1986，67，： | 1 |
| 12 | Orthodontic repositioning of lateral incisors into central incisors显示文摘 | McDowaU RJ Yar R Waring DT | 2012 | Br Dent J2012，212，9： | 1 |
| 13 | Management of giant hemangioma of the liver: resection versus observation 显示文摘 | Schnelldorfer T Ware AL Smoot R | 2010 | J Am Coil Surg2010，211，6： | 1 |
| 14 | Management of giant hemangioma of the liver: resection versus observation 显示文摘 | Schnelldorfer T Ware AL Smoot R | 2010 | J Am Coil Surg2010，211，6： | 1 |
| 15 | Management of giant hemanioma of the liver: resection versus observation 显示文摘 | Schnelldorfer T Ware AL Smoot R | 2010 | J Am Coil Surg2010，21，6： | 1 |
| 16 | A History of GPS sounding显示文摘 | Yunck T P Liu C H Ware R | 2000 | Special issue of Terre-strial Atmospheric and Oceanic Science2000，11，1： | 1 |
| 17 | Rock- en, Propagation Delays Induced in GPS Signals by Dry Air, Water Vapor, Hydrometeors and Other Particulates显示文摘 | Solheim F Vivekanandan J Ware R | 1997 | Journal of Geophysical Research1997，104，8： | 1 |
| 18 | How should children with speech sound disorders be classified? A review and critical evaluation of current classification systems显示文摘 | Waring R Knight R | 2013 | Lang commun disord2013，48，1： | 1 |
| 19 | NKT cell subsets can exert oppo- sing effects in autoimmunity, tumor surveillance and inflammation 显示文摘 | Viale R Ware R Maricic I | 2012 | Curr Immunol Rev2012，8，4： | 1 |
| 20 | Oxygen quenching of fluorescence in solution:an experimental study of the diffusion process显示文摘 | Ware W R | 1962 | J Phys Chem1962，66，： | 1 |
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