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| 1 | Qingnaoyizhi decoction suppresses the formation of glial fibrillary acidic protein-positive cells in cultured neural stem cells by inhibiting the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway显示文摘OBJECTIVE: Inactivation of the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3) signaling axis plays a crucial role in determining the fate of neural stem cells(NSCs).Qingnaoyizhi decoction(QNYZD) has been used for the treatment of vascular dementia and has shown to improve synaptic remodeling. The aim of this study was to evaluate the effect of cerebrospinal fluid(CSF) containing QNYZD(CSF-QNYZD) on the differentiation of cultured NSCs and the involvement of the JAK2/STAT3 pathway.METHODS: The protein expression levels of glial fibrillary acidic protein(GFAP), tubulin, drosophila mothers against decapentaplegic protein(SMAD-1), STAT3, and phosphorylated-STAT3 were detected by western immunoblot analysis in the groups: control, CSF, JAK/STAT inhibitor(AG490),CSF-QNYZD, and CSF-XDZ(CSF-Xidezhen). The differentiation of NSCs was determined by immunofluorescence staining. The proliferation of NSCs was measured using the Cell Counting Kit-8 proliferation assay.RESULTS: Compared with the control group,CSF-QNYZD and AG490 significantly increased the number and expression of tubulin-positive cells, reduced the number and expression of GFAP-positive cells, and down-regulated the expression of p-STAT3. However, CSF-QNYZD also decreased the expression of SMAD-1 and STAT3.CONCLUSION: Enhanced neuronal differentiation may be associated with the down-regulation of glial differentiation instead of promoting proliferationin treated NSCs. Furthermore, QNYZD may play a direct role in suppressing the formation of GFAP-positive cells and enhancing neuronal differentiation by inhibiting JAK2/STAT3 activation. Overall, these results provide insights into the possible mechanism underlying QNYZD-mediated neurogenesis. | Wu Yanqing Jing Zhiwei Qin Xiude Zhou Zhen Wang Kai Song Wanshan Wang Xueyan Hou Mengmeng Zhang Yulian Kang Liyuan | 2015 | Journal of Traditional Chinese Medicine2015，35，1： | 11 |
| 2 | Axonal autophagy during regeneration of the rat sciatic nerve显示文摘BACKGROUND: The removal of degenerated axonal debris during Wallerian degeneration is very important for nerve regeneration. However, the mechanism by which debris is removed is not been completely understood. Considerable controversy remains as to the clearance pathway and cells that are involved. OBJECTIVE: To investigate axonal autophagy during removal of degenerated axonal debris by transecting the sciatic nerve in a rat Wallerian degeneration model. DESIGN, TIME AND SETTING: Experimental neuropathological analysis. The experiment was conducted at the Laboratory Animal Service Center of the Southern Medical University between January and June 2005. MATERIALS: Fifty-four adult, Wistar rats of either sex, weighing 180-250 g, were obtained from the Laboratory Animal Service Center of the Southern Medical University. Animals were randomly divided into nine groups of six rats. METHODS: Wallerian degeneration was induced by transecting the rat sciatic nerve, and tissue samples from the distal stump were obtained 0.2, 0.4, 1, 2, 3, 4, 7, 10, and 15 days post-transection. Ultrathin sections were prepared for electron microscopy to study ultrastructure and enzyme cytochemistry staining. MAIN OUTCOME MEASURES: Ultrastructure (axon body, autophagic body, and cystoskeleton) of axons and myelin sheaths observed with electron microscopy; acidic phosphatase activity detected by Gomori staining using electron microscopy. RESULTS: The major changes of degenerating axons after transection were axoplasm swelling and separation of axons from their myelin sheath between five hours and two days post-transection. At four days post-transection, the axoplasm condensed and axons were completely separated from the myelin sheath, forming dissociative axon bodies. Vacuoles of different sizes formed in axons during the early phase after lesion. Larger dissociative axon bodies were formed when the axons were completely separated from the myelin sheath during a late phase. The axolemma surrounding the axon body was derived from the neuronal cell membrane; the condensed axoplasm contained many autophagic vacuoles at all levels. A large number of neurofilaments, microtubules, and microfilaments were arranged in a criss-cross pattern. The autophagic vacuoles exhibited acidic phosphatase activity. Axonal bodies were absorbed after degradation from day 7 onwards, and macrophages were observed rarely in the formative cavity. CONCLUSION: The degenerating axons were cleared mainly by axonal autophagy and Schwann cell phagocytosis during regeneration of the rat sciatic nerve, and macrophages exhibited only an assisting function. | Kangrong Lu Zhongxian Piao Zhenxi Liu Weiwang Gu Wanshan Wang Yingjie Piao | 2008 | Neural Regeneration Research2008，3，6： | 2 |
| 3 | The Integrated System of QFD and FMEA Based on CSCW显示文摘There has been some research on the theory of QFD(Quality Function Deployment)and FMEA(Failure Modes and Effect Analysis)recently,but little work to the integrated system of QFD and FMEA development.This paper analyses the technical difficulties of realization of QFD and proposes away using computer-supported collaborative work to develop the integrated system of QFD and FMEA and how to do it.The way can make the system more efficient and more suitable to new requirements of networked manufacturing enterprise.The same time,prototype system of the integrated system is developed by comprehensively ap- plication of Java、JSP、JavaServlet,the open World Wide Web platforms of which enhances the communication function,improves the group cooperation and shorts the development time.The system is verified reasonably and reliably through the example of QFD analysis of some automobile. | JIANG Xingyu GONG Yadong WANG Guihe YU Tianbiao WANG Wanshan School of Mechanical Engineering and Automation,Northeastern University,Shenyang 110004,China | 2006 | 武汉理工大学学报2006，28，S3： | 2 |
| 4 | Post-mortem tissue proteomics reveals the pathogenesis of multi-organ injuries of COVID-19显示文摘Multi-organ injuries are a major complication of severe Coronavirus Disease 2019(COVID-19).However,its pathogenesis is barely understood.Herein;we profile host responses to fatal SARS-CoV-2 infection by performing quantitative proteomics of 19 post-mortem samples of 8 different organs or tissues,including lungs,liver,intestine,kidney;spleen,brain,heart and muscle,from 3 deceased COVID-19 victims during the first wave of the pandemic(Supplementary Fig.S1A and B,Tables SI-S3). | Yang Qiu Di Wu Wanshan Ning Jiqian Xu Ting Shu Muhan Huang Rong Chen Jiancheng Zhang Yang Han Qingyu Yang Ruiting Li Xiaobo Yang Yaxin Wang Xiaojing Zou Shangwen Pan Chaolin Huang Yu Xue You Shang Xi Zhou | 2021 | National Science Review2021，8，11： | 1 |
| 5 | Research on rotary surface topography by orthogonal turn-milling显示文摘 | Zhu Lida Li Haonan Wang Wanshan | 2013 | The International Journal of Advanced Manufacturing Technology2013，69，9101112： | 1 |
| 6 | Finite Element Simulation of Chip Formation with Single Grain显示文摘 | Wang Wanshan Su Chong Pang Zirui | 2009 | Key Engineering Material2009，416，： | 1 |
| 7 | Partner selection and optimization model in global manufacturing显示文摘 | DONG Wenhui GONG Yadong WANG Wanshan | 2005 | Journal of Northeastern University2005，26，5： | 1 |
| 8 | Pretreatment with edaravone reduces lung mitochondrial damage in an infant rabbit ischemia-reperfusion model 显示文摘 | Wanshan Qiu Haiyong Gu Liang Zheng | 2008 | J Pediatr Surg2008，43，11： | 1 |
| 9 | On Quadratic Vectorial Bent Functions in Trace Forms显示文摘By permuta tion behavior of cer tain linearized polynomials,the bentness of quadratic vectorial k—1 it bent functions of the form F(x)=∑i=1^k-1Trm^n(Cix1+2it)+Trm^kt(ckx1+2kt)is investigated,where n=2kt and m|kt with k,t being positive integers.The numerical results show t hat there exis t new quadratic vec to rial bent functions obtained up to extended affine equivalence. | ZHOU Junchao XU Yunge ZHANG Wanshan | 2020 | Chinese Journal of Electronics2020，29，5： | 1 |
| 10 | Hypoxia-induced overexpression of DEC1 is regulated by HIF-1α in hepatocellularcarcinoma显示文摘 | Wanshan Ma Xiaohong Shi Sumei Lu Linlin Wu Yunshan Wang | 2013 | Oncology Reports2013，，6： | 1 |
| 11 | Comparison between adult and infant lung injury in a rabbit ischemia-reperfusion model显示文摘 | Wanshan Qiu Liang Zheng Haiyong Gu Duan Chen Yijiang Chen | 2008 | The Journal of Thoracic and Cardiovascular Surgery2008，，： | 1 |
| 12 | WocEA:The visualization of functional enrichment results in word clouds显示文摘The integration,analysis and visualization of the big omics data are critical for addressing a broad spectrum of biological questions.One of the most frequently conducted procedures is enrichment analysis,which statistically tests whether individual functional annotations of Gene Ontology (GO)or Kyoto Encyclopedia of | Wanshan Ning Shaofeng Lin Jiaqi Zhou Yaping Guo Ying Zhang Di Peng Wankun Deng Yu Xue | 2018 | Journal of Genetics and Genomics2018，45，7： | 1 |
| 13 | Finite element analysis of metal cutting process base on LS-DYNA显示文摘 | Hou Junming Zhu Lida Wang Wanshan | | 0，，： | 1 |
| 14 | Part- ner selection and optimization model in global manufactur- ing 显示文摘 | DONG Wenhui GONG Yadong WANG Wanshan | 2005 | Journal of Northeastern University2005，26，5： | 1 |
| 15 | Integration of fuzzy AHP and FPP with TOPSIS methodology for aero- engine health assessment 显示文摘 | Wang Jianrong Fan Kai Wang Wanshan | 2010 | Expert Systems with Applications2010，37，12： | 1 |
| 16 | Integration of fuzzy AHP and FPP with TOPSIS methodology for aero engine health assessment显示文摘 | Wang Jianrong Fan Kai Wang Wanshan | 2010 | Expert Systems with Applications2010，37，12： | 1 |
| 17 | Integra-ting analytic hierarchy process and genetic algorithm for air-craft engine maintenance scheduling problem显示文摘 | WANG Jianrong YU Tianbiao WANG Wanshan | 2010 | Advances inIntelligent and Soft Computing2010，66，： | 1 |
| 18 | ADI-FDTDMethod for Two-Dimensional Transient Electromagnetic Problems显示文摘An efficient and accurate numerical scheme is proposed for solving the transverse electric(TE)mode electromagnetic(EM)propagation problemin two-dimensional earth.The scheme is based on the alternating direction finite-difference time-domain(ADI-FDTD)method.Unlike the conventional upward continuation approach for the earth-air interface,an integral formulation for the interface boundary is developed and it is effectively incorporated to the ADI solver.Stability and convergence analysis together with an error estimate are presented.Numerical simulations are carried out to validate the proposed method,and the advantage of the present method over the popular Du-Fort-Frankel scheme is clearly demonstrated.Examples of the electromagnetic field propagation in the ground with anomaly further verify the effectiveness of the proposed scheme. | Wanshan Li Yile Zhang Yau Shu Wong Dong Liang | 2016 | Communications in Computational Physics2016，19，1： | 0 |
| 19 | Bioinspired mineral-in-shell nanoarchitectonics:Functional empowerment of mineral precursors for guiding intradentinal mineralization显示文摘Effective mineralization of biological structures poses a significant challenge in hard tissue engineering as it necessitates overcoming geometric complexities and multistep biomineralization processes.In this regard,we propose“mineral-in-shell nanoarchitectonics”,inspired by the nanostructure of matrix vesicles,which can influence multiple mineralization pathways.Our nanostructural design empowers mineral precursors with tailorable properties through encapsulating amorphous calcium phosphate within a multifunctional tannic acid(TA)and silk fibroin(SF)nanoshell.The bioinspired nanosystem facilitates efficient recruitment of mineral precursors throughout the dentin structures,followed by large-scale intradentinal mineralization both in vitro and in vivo,which provides persistent protection against external stimuli.Theoretical simulations combined with experimental studies attribute the success of intradentinal mineralization to the TA-SF nanoshell,which exhibits a strong affinity for the dentin structure,stabilizing amorphous precursors and thereby facilitating concomitant mineral formation.Overall,this bioinspired mineral-in-shell nanoarchitectonics shows a promising prospect for hard tissue repair and serves as a blueprint for next-generation biomineralization-associated materials. | Xiaoran Zheng Yang Liu Mingjing Li Yuyan Li Wanshan Gao Rongmin Qiu Jiaqi Xing Jiaojiao Yang Yantao Chen Xinyuan Xu Mingming Ding Jun Luo Jianshu Li | 2024 | Nano Research2024，17，5： | 0 |
| 20 | An oxygen reservoir-irrigated photoimmunotherapy of malignant melanoma显示文摘Tumor hypoxia is the pivotal factor limiting the therapeutic efficacy of photodynamic therapy(PDT),and can be partly improved by either the oxygen economizing or the oxygen supplementation strategies.Nevertheless,the current studies scarcely integrated the merits of both strategies and neglected the bottleneck of poor oxygen infiltration in deep tumors,resulting in PDT resistance.Herein,we developed an oxygen reservoir-irrigated PDT which integrates oxygen supply,oxygen economizing,and oxygen infiltration altogether.Specifically,mitochondria-targeted mesoporous prussian blue nanoparticles(Ce6@TPB)were fabricated to bridge the gap between oxygen economizing and oxygen supplementation by reducing oxygen output while increasing oxygen input.Hyaluronidase-loaded microneedles were further developed to pave the way for deep PDT with increased infusion of oxygen and photosensitizer by degrading dense extracellular matrix.The modulation of tumor oxygenation and permeability during PDT leads to the complete eradication of primary melanoma and strong immunogenic cell death.Its further combination with checkpoint-blockade inhibitor greatly suppressed the proliferation of distal tumors by reprogramming immune microenvironments,as evidenced by the depletion of M2 macrophage,increased infiltration of anti-tumor immune cells,and elevated excretion of immune cytokines.Therefore,such an oxygen reservoir-irrigated PDT potentiates powerful photoimmunotherapy and provides a favorable prospect for tumor treatment. | Yao Huang Tingting Peng Yangyan Chen Fapeng Zhang Wanshan Hu XinYi Gao Hongyu Chen Ping Tang Chuanbin Wu Xin Pan | 2023 | Nano Research2023，16，2： | 0 |
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