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| 1 | Expression of co-stimulator 4-1BB molecule in hepatocellular carcinoma and adjacent non-tumor liver tissue,and its possible role in tumor immunity显示文摘AIM:To investigate the expression of 4-1BB molecule in hepatocellular carcinoma (HCC) and its adjacent tissues.METHODS:Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the gene expression of 4-1BB in hepatocarcinoma and its adjacent tissues,and peripheral blood mononuclear cells (PBMCs) from both HCC and health control groups.Flow cytometry was used to analyse the phenotypes of T cell subsets from the blood of HCC patients and healthy volunteers,and further to determine whether 4-1BB molecules were also expressed on the surface of CD4+ and CD8+ T cells. The localization of 4-1BB proteins on tumor infiltrating T cells was determined by direct immunofluorescence cytochemical staining and detected by confocal microscopy.RESULTS:4-1BB mRNA, which was not detectable in normal liver,was found in 19 liver tissues adjacent to tumor edge(<1.0cm).Low expression of 4-1BB mRNA was shown in 8 tumor tissues and 6 liver tissues located within 1 to 5cm away from tumor edge. In PBMCs, 4-1BB mRNA was almost not detected. Percentage of CD4^+, CD8^+ and CD3^+/CD25^+ T cells, as well as ratio of CD4 to CD8 revealed no difference between groups (P>0.05,respectively),while a significant lower percentage of CD3^+ T cell was found in HCC group as compared to healthy control group (P<0.05).However, 4-1BB molecules were almost not found on the surface of CD4+ and CD8+ T cells in HCC and healthy control group.Double-staining of 4-1BB^+/CD4^+ and 4-1BB^+/CD8^+ immunofluorescence on tumor infiltrating T cells was detected in 13 liver tissues adjacent to tumor edge (<1.0cm) by confocal microscopy.CONCLUSION: Although HCC may escape from immune attack by weak immunogenicity or downregulated expression of MHC-1 molecules on the tumor cell surface,tumor infiltrating T cells can be activated via other costimulatory signal pathways to exert a limited antitumor effect on local microenvironment. The present study also implicates that modulating 4-1BB/4-1BBL costimulatory pathway may be an effective immunotherapy strategy to augment the host response. | Wan, YL Zheng, SS Zhao, ZC Li, MW Jia, CK Zhang, H | 2004 | World Journal of Gastroenterology2004，10，2： | 6 |
| 2 | Smoking among young rural to urban migrant women in China: a cross-sectional survey 显示文摘 | Wan X Shin SS Wang Q | 2011 | PLoS One2011，6，23： | 1 |
| 3 | The RNA component of human telomeres显示文摘 | Feng JL Funk WD Wan SS | 1995 | Science1995，269，： | 1 |
| 4 | Rett syndrome and beyond:recurrent spontaneous and familial MECP2 mutations at CpG hotspots显示文摘 | Wan M Lee SS Zhang X | | 0，，： | 1 |
| 5 | Analysis of the efficacy of three treatment options for cesarean scar pregnancy management显示文摘 | Li YR Xiao SS Wan YJ | 2014 | Obstet Gynaecol Res2014，40，11： | 1 |
| 6 | Spectrum of MECP2 mutations in Rett syndrome 显示文摘 | Lee SS Wan M Francke U | 2001 | Brain Dev2001，，1： | 1 |
| 7 | Globoidnan A:a lignan from Eucalyptus globoidea inhibits HIV integrase显示文摘 | Yu J Wan SS | 2004 | Phytochemistry2004，65，： | 1 |
| 8 | Risk of malignancy in women who underwent hysterectomy for uterine prolapse 显示文摘 | Wan OY Cheung RY Chan SS | 2013 | Aust N Z J Obstet Gynaecol2013，53，： | 1 |
| 9 | Risk of malignancy in women who underwent hysterectomy for uterine prolapse显示文摘 | Wan OY Cheung RY Chan SS | 2013 | Aust N Z J Obstet Gynaecol2013，53，2： | 1 |
| 10 | Bm-TFF2, a trefoil factor protein with platelet activation activity from frog Bombina maxima skin secretions 显示文摘 | Zhang J Zhang Y Wan SG Wei SS Lee WH 2005 | 2005 | Biochem Biophys Res Commun2005，330，4： | 1 |
| 11 | Negative regulation of the RelA/p65 transactivation function by the product of the DEK proto-oncogene显示文摘 | Sammons M Wan SS Vogel NL | 2006 | J Biol Chem2006，281，26： | 1 |
| 12 | Application of infrequent - restriction - site PCR to clinical isolates of Acinetobacter baumannii and Serratia marcescens显示文摘 | Jin HY Jung HC Wan SS | 1999 | J Clin Microbiol1999，37，10： | 1 |
| 13 | Bm-TFF2, a trefoil factor protein with platelet activation activity from frog Bombina maxima skin secretions 显示文摘 | Zhang J Zhang Y Wan SG Wei SS Lee WH 2005 | 2005 | Biochem Biophys Res Commun2005，330，4： | 1 |
| 14 | Globoidnan A:a lignan from Eucalyptus globoidea inhibits HIV integrase 显示文摘 | Ovenden SP Yu J Wan SS | 2004 | Phytochemistry2004，65，24： | 1 |
| 15 | PACS-A defines a novel gene family of cytosolic sortingproteins required for trans-Golgi network localizationg显示文摘 | Wan L Molloy SS Thomas | 1998 | Cell1998，94，： | 1 |
| 16 | Rett syndrome and beyond:recurrent spontaneous and familial MECP2 mutations at CpG hotspots显示文摘 | Wan M Lee SS Zhang X | 1999 | Am J Hum Genet1999，65，6： | 1 |
| 17 | Perceived discrimination and smoking among rural-to-urban migrant women working as restau- rant/hotel workers and sex workers in China 显示文摘 | Shin SS Wan X Wang Q | 2013 | J Immigr Minor Health2013，15，1： | 1 |
| 18 | Analysis of the efficacy of three treatment options for cesarean sear pregnancy management 显示文摘 | Li YR Xiao SS Wan YJ | 2014 | J Obstet Gynaecol Res2014，40，11： | 1 |
| 19 | The effects of hydrocolloid dressing and gentian violet on radiation-induced moist desqumation wound healing显示文摘 | Mak SS Molassiotis A Wan WM | 2000 | Cancer Nurs2000，23，： | 1 |
| 20 | Smoking among young rural to urban migrant women in China: a cross-sectional suey 显示文摘 | Wan X Shin SS Wang Q | 2011 | PLoS One2011，6，23： | 1 |
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