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| 1 | 2005—2007年儿科分离的流感嗜血杆菌、肺炎链球菌(包括血清型19A)和卡他莫拉菌对常用抗菌药物的敏感性显示文摘 | Harrison CJ Woods C Stout G 周颖杰 王明贵 | 2010 | 中国感染与化疗杂志2010，10，1： | 5 |
| 2 | Screening Helicobacter pylori genes induced during infection of mouse stomachs显示文摘AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(H.pylori) as it relates to its survival in the host.METHODS:In vivo expression technology(IVET) systems are used to identify microbial virulence genes.We modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and pIVET12.Our novel IVET systems were developed by the fusion of random Sau3A DNA fragments of H.pylori and a tandem-reporter system of chloramphenicol acetyltransferase and beta-galactosidase.Additionally,each vector contains a kanamycin resistance gene.We used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that H.pylori expresses in vivo.Gene expression studies were conducted by infecting RAW 264.7 cells with H.pylori.This was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced genes.RESULTS:In this study,we have identified 31 in vivo induced(ivi) genes in the initial screens.These 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse stomachs.Virulence factors,vacA and cagA,were found in this screen and are known to play important roles in H.pylori infection,colonization and pathogenesis.Their detection validates the efficacy of these screening systems.Some of the identified ivi genes have already been implicated to play an important role in the pathogenesis of H.pylori and other bacterial pathogens such as Escherichia coli and Vibrio cholerae.Transcription profiles of allivi genes were confirmed by real time PCR analysis of H.pylori RNA isolated from H.pylori infected RAW 264.7 macrophages.We compared the expression profile of H.pylori and RAW 264.7 coculture with that of H.pylori only.Some genes such as cag A,vac A,lpx C,mur I,tlp C,trx B,sod B,tnp B,pgi,rbf A and inf B showed a 2-20 fold upregulation.Statistically significant upregulation was obtained for all the above mentioned genes(P < 0.05).tlp C,cag A,vac A,sod B,rbf A,inf B,tnp B,lpx C and mur I were also significantly upregulated(P < 0.01).These data suggest a strong correlation between results obtained in vitro in the macrophage cell line and in the intact animal.CONCLUSION:The positive identification of these genes demonstrates that our IVET systems are powerful tools for studying H.pylori gene expression in the host environment. | Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood Yisheng Ni Kimberly Smith Sharon H Jackson William G Coleman | 2012 | World Journal of Gastroenterology2012，18，32： | 5 |
| 3 | The role of cytoreductive nephrectomy in the management of metastatic renal cell carcinoma显示文摘 | Christopher G Wood | 2003 | Urologic Clinics of North America2003，，3： | 2 |
| 4 | Dendrite crystallization: Numerical study &the one-sided model显示文摘 | SAITO Y WOOD G HMULLER K | 1987 | Phys Rev Lett1987，58，： | 1 |
| 5 | The current status of poilcystic ovary syndrome显示文摘 | Kovacs G Wood C | 2001 | Aust NZ J Obstet Gyaecol2001，41，1： | 1 |
| 6 | Effects of breed,diet and muscle on fat deposition and eating quality in pigs显示文摘 | Wood J D Nute G R Richardson R I | 2004 | Meat Science2004，67，4： | 1 |
| 7 | The effects of commonly prescribed premolar extraction sequences on the curvature of the upper and lower lips显示文摘 | Wholley C J Woods M G | 2003 | Angle Orthod2003，73，4： | 1 |
| 8 | Suppression of tu- morigenicity of breast cancer cells by an epithelial cell adhe- sion molecule (C-CAM1): the adhesion and growth suppression are mediated by different domains显示文摘 | LUO W WOOD C G EARLEY K | 1997 | Oncngene1997，14，14： | 1 |
| 9 | Film formation and detachment during anodizing of Al-Mg alloys显示文摘 | ZHOU X THOMPSON G E SKELDON P WOOD G C SHIMIZU K HABAZAKI H | 1999 | Corrosion Science1999，41，8： | 1 |
| 10 | ASCO/SSO re-view of current role of risk-reducing surgery in common he-reditary cancer syndromes显示文摘 | Guillem J G Wood W C Moley J F | 2006 | J Clin Oncol2006，24，28： | 1 |
| 11 | Electron microscopy of ion beam thinned porous anodic films formed on aluminum显示文摘 | THOMPSON G E FUNEAUX R C WOOD G C | 1978 | Corros Sci1978，18，： | 1 |
| 12 | The morphology and the mechanism of formation of porous anodic films on aluminium显示文摘 | O'Sullivan T Wood G C | 1970 | Proceed Royal Soc London Series A1970，317，： | 1 |
| 13 | Characterization of Arabidopsis AtAMT2, a high-affinity ammonium transporter of the plasma membrane 显示文摘 | Sohlenkamp C Wood C C Roeb G W | 2002 | Plant Physiology2002，130，4： | 1 |
| 14 | Complex event detection at wire speed with FPGAs 显示文摘 | WOODS L TEUBNER J ALONSO G | 2010 | Proceedings of the VLDB Endowment2010，3，12： | 1 |
| 15 | Mechanically alloying of iron-aluminium intermetallies 显示文摘 | Fair G H Wood J V | 1993 | Powder Metallurgy1993，36，2： | 1 |
| 16 | Impedance and electrochemical noise measurements on iron and iron-carbon alloys in hot caustic soda 显示文摘 | FLIS J DAWSON J L GILL J WOOD G C | 1991 | Corrosion Science1991，32，8： | 1 |
| 17 | The discrete-time quadraturesubsample estimation of delay 显示文摘 | Maskell D L Woods G S | 2002 | IEEE Transaction onInstrumentation and Measurement2002，51，1： | 1 |
| 18 | Protection of retinal ganglion cells and the optic nmve during short-term hyperglycemia in experimental glaucoma显示文摘 | Ebneter A Chidlow G Wood JP | | 0，，： | 1 |
| 19 | An avian influenza vires of H10 subtype that is highly pathogenic for chickens, but lacks multiple basic amino acids at the haemagglutinin cleavage site 显示文摘 | Wood G W Banks J Strong I | 1996 | Avian Pathol1996，25，4： | 1 |
| 20 | The effects of business uni- versity alliance on innovative output and financial performance: A study of publicly traded biotechnology companies 显示文摘 | GEORGE G ZAHRA S A WOOD D | 2002 | Joumal of Business Venturing2002，17，3： | 1 |
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