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| 1 | RANK-ligand and osteoprotegerin as biomarkers in the differentiation between periprosthetic joint infection and aseptic prosthesis loosening显示文摘AIM To assess serum levels of RANK-ligand(RANKL) and osteoprotegerin(OPG) as biomarkers for periprosthetic joint infection(PJI) and compare their accuracy with standard tests.METHODS One hundred and twenty patients presenting with a painful total knee or hip arthroplasty with indication for surgical revision were included in this prospective clinical trial. Based on standard diagnostics(joint aspirate, microbiological, and histological samples) and Musculoskeletal Infection Society consensus classification,patients were categorized into PJI, aseptic loosening,and control groups. Implant loosening was assessed radiographically and intraoperatively. Preoperative serum samples were collected and analyzed for RANKL, OPG, calcium, phosphate, alkaline phosphatase(AP), and the bone-specific subform of AP(b AP). Statistical analysis was carried out, testing for significant differences between the three groups and between stable and loose implants. RESULTS All three groups were identical in regards to age, gender, and joint distribution. No statistically significant differences in the serum concentration of RANKL(P = 0.16) and OPG(P = 0.45) were found between aseptic loosening and PJI, with a trend towards lower RANKL concentrations and higher OPG concentrations in the PJI group. The RANKL/OPG ratio was significant for the comparison between PJI and non-PJI(P = 0.005). A ratio > 60 ruled out PJI in all cases(specificity: 100%, 95%CI: 89, 11% to 100.0%) but only 30% of non-PJI patients crossed this threshold. The positive predictive value remained poor at any cut-off. In the differentiation between stable and loose implants, none of the parameters measured(calcium, phosphate, AP, and b AP) showed a significant difference, and only AP and b AP measurements showed a tendency towards higher values in the loosened group(with P = 0.09 for AP and P = 0.19 for b AP). CONCLUSION Lower RANKL and higher OPG concentrations could be detected in PJI, without statistical significance. | Max J Friedrich Matthias D Wimmer Jan Schmolders Andreas C Strauss Milena M Ploeger Hendrik Kohlhof Dieter C Wirtz Sascha Gravius Thomas M Randau | 2017 | World Journal of Orthopedics2017，8，4： | 4 |
| 2 | Role of interleukin-1 and its antagonism of hepatic stellate cell proliferation and liver fibrosis in the Abcb4^(-/-) mouse model显示文摘AIM: To study the interleukin-1(IL-1) pathway as a therapeutic target for liver fibrosis in vitro and in vivo using the ATP-binding cassette transporter b4^(-/-)(Abcb4^(-/-)) mouse model.METHODS: Female and male Abcb4^(-/-) mice from 6 to 13 mo of age were analysed for the degree of cholestasis(liver serum tests), extent of liver fibrosis(hydroxyproline content and Sirius red staining) and tissue-specific activation of signalling pathways such as the IL-1 pathway [quantitative polymerase chain reaction(q PCR)]. For in vivo experiments, murine hepatic stellate cells(HSCs) were isolated via pronasecollagenase perfusion followed by density gradient centrifugation using female mice. Murine HSCs were stimulated with up to 1 ng/m L IL-1β with or without 2.5 μg/m L Anakinra, an IL-1 receptor antagonist, respectively. The proliferation of murine HSCs was assessed via the Brd U assay. The toxicity of Anakinra was evaluated via the fluorescein diacetate hydrolysis(FDH) assay. In vivo 8-wk-old Abcb4^(-/-) mice with an already fully established hepatic phenotype were treated with Anakinra(1 mg/kg body-weight daily intraperitoneally) or vehicle and liver injury and liver fibrosis were evaluated via serum tests, q PCR, hydroxyproline content and Sirius red staining. RESULTS: Liver fibrosis was less pronounced in males than in female Abcb4^(-/-) animals as defined by a lower hydroxyproline content(274 ± 64 μg/g vs 436 ± 80 μg/g liver, respectively; n = 13-15; P < 0.001; MannWhitney U-test) and lower m RNA expression of the profibrogenic tissue inhibitor of metalloproteinase-1(TIMP)(1 ± 0.41 vs 0.66 ± 0.33 fold, respectively; n = 13-15; P < 0.05; Mann-Whitney U-test). Reduced liver fibrosis was associated with significantly lower levels of F4/80 m RNA expression(1 ± 0.28 vs 0.71 ± 0.41 fold, respectively; n = 12-15; P < 0.05; Mann-Whitney U-test) and significantly lower IL-1β m RNA expression levels(1 ± 0.38 vs 0.44 ± 0.26 fold, respectively; n = 13-15; P < 0.001; Mann-Whitney U-test). No gender differences in the serum liver parameters [bilirubin; alanine aminotransferase(ALT); aspartate aminotransferase and alkaline phosphatase(AP)] were found. In vitro, the administration of IL-1β resulted in a significant increase in HSC proliferation [0.94 ± 0.72 arbitrary units(A.U.) in untreated controls, 1.12 ± 0.80 A.U. at an IL-1β concentration of 0.1 ng/m L and 1.18 ± 0.73 A.U. at an IL-1β concentration of 1 ng/m L in samples from n = 6 donor animals; P < 0.001; analyses of variance(ANOVA)]. Proliferation was reduced significantly by the addition of 2.5 μg/m L Anakinra(0.81 ± 0.60 A.U. in untreated controls, 0.92 ± 0.68 A.U. at an IL-1β concentration of 0.1 ng/m L, and 0.91 ± 0.69 A.U. at an IL-1β concentration of 1 ng/m L; in samples from n = 6 donor animals; P < 0.001; ANOVA) suggesting an anti-proliferative effect of this clinically approved IL-1 receptor antagonist. The FDH assay showed this dose to be non-toxic in HSCs. In vivo, Anakinra had no effect on the hepatic hydroxyprolinecontent, liver serum tests(ALT and AP) and profibrotic(collagen 1α1, collagen 1α2, transforming growth factor-β, and TIMP-1) and anti-fibrotic [matrix metalloproteinase 2(MMP2), MMP9 and MMP13 ] gene expression after 4 wk of treatment. Furthermore, the hepatic IL-1β and F4/80 m RNA expression levels were unaffected by Anakinra treatment.CONCLUSION: IL-1β expression is associated with the degree of liver fibrosis in Abcb4^(-/-) mice and promotes HSC proliferation. IL-1 antagonism shows antifibrotic effects in vitro but not in Abcb4^(-/-) mice. | Florian P Reiter Ralf Wimmer Lena Wottke Renate Artmann Jutta M Nagel Manuel O Carranza Doris Mayr Christian Rust Peter Fickert Michael Trauner Alexander L Gerbes Simon Hohenester Gerald U Denk | 2016 | World Journal of Hepatology2016，8，8： | 3 |
| 3 | A bright future for the tree shrew in neuroscience research:Summary from the inaugural Tree Shrew Users Meeting显示文摘Tree shrews(Tupaia spp.)have been used in neuroscience research since the 1960s due to their evolutionary proximity to primates.The use of and interest in this animal model have recently increased,in part due to the adaptation of modern neuroscience tools in this species.These tools include quantitative behavioral assays,calcium imaging,optogenetics and transgenics.To facilitate the exchange and development of these new technologies and associated research findings,we organized the inaugural“Tree Shrew Users Meeting”which was held online due to the COVID-19 pandemic.Here,we review this meeting and discuss the history of tree shrews as an animal model in neuroscience research and summarize the current themes being investigated using this animal,as well as future directions. | Elise Savier Madineh Sedigh-Sarvestani Ralf Wimmer David Fitzpatrick | 2021 | Zoological Research2021，42，4： | 3 |
| 4 | 奥钢联近终形异型坯浇铸技术显示文摘奥钢联近终形异型坯浇铸对于生产大、中型型钢及工字梁,与薄板坯生产扁材产品具有同样的优点。为了从异型坯技术获得更大效益,连铸机必须与轧机直接连接。该技术的经济效益主要包括更低的轧制成本、更高的生产率和降低能耗。因此,近终形异型坯浇铸是传统的方坯浇铸生产H型钢方法的一个极好的替代方案。 | FRANZ Leingruber CHRISTIAN Chimani FRANZ Wimmer | 2004 | 连铸2004，29，5： | 3 |
| 5 | Testing methods for 1100 kV UHVDC transformer显示文摘1 Development of UHVDC transmission capabilities The economical development of China is closely connected with safe and reliable power supply.Load centers e.g.in central and eastern China need huge amounts of electrical power.Available energy resources and consumption areas are often distributed inverse.As a consequence it is necessary to import electrical power to load center areas in an economic and efficient way. | Thomas Hammer Rene Wimmer Karsten Loppach Ronny Fritsche | 2018 | Global Energy Interconnection2018，1，2： | 2 |
| 6 | Scale invariant texture descriptors for classifying celiac disease显示文摘 | G. Wimmer S. Hegenbart A. Uhl A. Vécsei | 2013 | Medical Image Analysis2013，，： | 2 |
| 7 | Quest for the best endoscopic imaging modality for computer-assisted colonic polyp staging显示文摘BACKGROUND It was shown in previous studies that high definition endoscopy, high magnification endoscopy and image enhancement technologies, such as chromoendoscopy and digital chromoendoscopy [narrow-band imaging(NBI), iScan] facilitate the detection and classification of colonic polyps during endoscopic sessions. However, there are no comprehensive studies so far that analyze which endoscopic imaging modalities facilitate the automated classification of colonic polyps. In this work, we investigate the impact of endoscopic imaging modalities on the results of computer-assisted diagnosis systems for colonic polyp staging.AIM To assess which endoscopic imaging modalities are best suited for the computerassisted staging of colonic polyps.METHODS In our experiments, we apply twelve state-of-the-art feature extraction methods for the classification of colonic polyps to five endoscopic image databases of colonic lesions. For this purpose, we employ a specifically designed experimental setup to avoid biases in the outcomes caused by differing numbers of images per image database. The image databases were obtained using different imaging modalities. Two databases were obtained by high-definition endoscopy in combination with i-Scan technology(one with chromoendoscopy and one without chromoendoscopy). Three databases were obtained by highmagnification endoscopy(two databases using narrow band imaging and one using chromoendoscopy). The lesions are categorized into non-neoplastic and neoplastic according to the histological diagnosis.RESULTS Generally, it is feature-dependent which imaging modalities achieve high results and which do not. For the high-definition image databases, we achieved overall classification rates of up to 79.2% with chromoendoscopy and 88.9% without chromoendoscopy. In the case of the database obtained by high-magnification chromoendoscopy, the classification rates were up to 81.4%. For the combination of high-magnification endoscopy with NBI, results of up to 97.4% for one database and up to 84% for the other were achieved. Non-neoplastic lesions were classified more accurately in general than non-neoplastic lesions. It was shown that the image recording conditions highly affect the performance of automated diagnosis systems and partly contribute to a stronger effect on the staging results than the used imaging modality.CONCLUSION Chromoendoscopy has a negative impact on the results of the methods. NBI is better suited than chromoendoscopy. High-definition and high-magnification endoscopy are equally suited. | Georg Wimmer Michael Gadermayr Gernot Wolkersdorfer Roland Kwitt Toru Tamaki Jens Tischendorf Michael Hafner Shigeto Yoshida Shinji Tanaka Dorit Merhof Andreas Uhl | 2019 | World Journal of Gastroenterology2019，25，10： | 2 |
| 8 | The PG500 series: novel heparan sulfate mimetics as potent angiogenesis and heparanase inhibitors for cancer therapy显示文摘 | K. Dredge E. Hammond K. Davis C. P. Li L. Liu K. Johnstone P. Handley N. Wimmer T. J. Gonda A. Gautam V. Ferro I. Bytheway | 2010 | Investigational New Drugs2010，，3： | 2 |
| 9 | A versatile vector set for animal transgenesis显示文摘 | Horn C Wimmer E A | 2000 | Dev Genes Evol2000，210，： | 1 |
| 10 | Management of postoperative wound infec- tion in posterior spinal fusion with instrumentation 显示文摘 | Wimmer C Gluch H | 1996 | J Spinal Disord1996，9，6： | 1 |
| 11 | Stable transformation of a Mamestra brassicae (lepidoptera) cell line with the lepidopteran-derived transposon piggyBac 显示文摘 | Mandrioli M Wimmer EA | 2003 | Insect Biochem Mol Biol2003，33，1： | 1 |
| 12 | Relation between depression and anxiety in dystonic patients: implications for clinical management 显示文摘 | MORARU E SCHNIDER P WIMMER A | 2002 | Depress and Anxiety2002，16，3： | 1 |
| 13 | Gas chro- matography analysis of blackcurrant oil in relation to its stability 显示文摘 | STRANSKY K ZAREVUCKA M WIMMER Z | 2005 | Food Chem2005，92，3： | 1 |
| 14 | Boron-and-salt interactions in wheat areaffected by boron supply显示文摘 | Wimmer M A Goldbach H E | 2012 | Journal of Plant Nutrition and Soil Science、2012，175，2： | 1 |
| 15 | Progression in lacunar stroke is related to elevated acute phase parameters显示文摘 | Audebert HJ Pellkofer TS Wimmer ML | 2004 | Eur Neurol2004，51，3： | 1 |
| 16 | Radiofrequency catheter ablation of chronic atrial fibrillation guided by complex electrograms显示文摘 | Crawford TC Wimmer A Dey S | 2008 | J Interv Card Eleetrophysiol2008，21，1： | 1 |
| 17 | Coping with disease by the patient : Results of a medico- sociological study of the coping process by cancer patients and possibilities for support by the physician显示文摘 | Wimmer H | 1991 | Wien Med Woehensehr1991，141，4： | 1 |
| 18 | Topology-based interlocking of electrical substations显示文摘 | Kopainsky J Wimmer W Brand K P | 1986 | IEEE Transactions on Power Delivery1986，1，3： | 1 |
| 19 | Progression in lacunar stroke is releted to e|evated acute phase parameters显示文摘 | Audebert HJ Pellkofer TS Wimmer ML | 2004 | Eur Neurol2004，51，3： | 1 |
| 20 | The influence of temperature on latewood lignip content in treeline Norway spruce compared with maximum density and ring width 显示文摘 | Wolfgang Gindl Michael Grabner Rupert Wimmer | 2000 | Trees2000，14，7： | 1 |
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