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| 1 | Development of a Chinese land data assimilation system:its progress and prospects显示文摘陆地数据吸收的目的是为改进陆地表面状态的评价把多来源观察合成陆地表面模型的动力学。我们为中国的陆地领土开发了一个陆地数据吸收系统。在这个系统,普通陆地模型和简单生物圈,模型 2 被用来模仿陆地表面过程。当转移的观察操作员为预言建模进估计的亮度温度,融化并且结冰的土壤,雪,湖,和植被的放射的转移模型被使用。一个 Monte-Carlo 顺序的过滤器,整体 Kalman 过滤器,作为数据吸收方法被实现集成当模特儿并且观察。系统能够遥远地吸收被动微波察觉到的数据象特殊传感器微波成像器那样(SSM 我) , TRMM 微波成像器(TMI ) ,和先进微波扫描辐射计为曙光女神(AMSRE ) 提高了并且在土壤和雪的 situmeasurements 常规。与 0.25 度的一个空间决定和一个小时的时间的分辨率,为土壤潮湿,土壤温度,雪和冻结的土壤的空间与时间地一致的吸收数据集被生产了。这篇论文介绍在数据吸收算法,陆地表面水文学变量的陆地表面建模微波遥感,和大气的强迫的数据的准备上做的中国陆地数据吸收系统和进步的开发。为未来开发开发陆地数据吸收系统和观点的不同特征和挑战也被讨论。 | Li Xin Huang Chunlin Che Tao Jin Rui Wang Shugong Wang Jiemin Gao Feng Zhang Shuwen Qiu Chongjian Wang Chenghai | 2007 | Progress in Natural Science:Materials International2007，17，8： | 30 |
| 2 | Neuroprotective effects of rutaecarpine on cerebral ischemia reperfusion injury显示文摘Rutaecarpine,an active component of the traditional Chinese medicine Tetradium ruticarpum,has been shown to improve myocardial ischemia reperfusion injury.Because both cardiovascular and cerebrovascular diseases are forms of ischemic vascular disease,they are closely related.We hypothesized that rutaecarpine also has neuroprotective effects on cerebral ischemia reperfusion injury.A cerebral ischemia reperfusion model was established after 84,252 and 504 μg/kg rutaecarpine were given to mice via intraperitoneal injection,daily for 7 days.Results of the step through test,2,3,5-triphenyl tetrazolium chloride dyeing and oxidative stress indicators showed that rutaecarpine could improve learning and memory ability,neurological symptoms and reduce infarction volume and cerebral water content in mice with cerebral ischemia reperfusion injury.Rutaecarpine could significantly decrease the malondialdehyde content and increase the activities of superoxide dismutase and glutathione peroxidase in mouse brain.Therefore,rutaecarpine could improve neurological function following injury induced by cerebral ischemia reperfusion,and the mechanism of this improvement may be associated with oxidative stress.These results verify that rutaecarpine has neuroprotective effects on cerebral ischemia reperfusion in mice. | Chunlin Yan Ji Zhang Shu Wang Guiping Xue Yong Hou | 2013 | Neural Regeneration Research2013，8，22： | 20 |
| 3 | Down.regulation of E.cadherin enhances prostate cancer chemoresistance via Notch signaling显示文摘Background:The chemoresistance of prostate cancer(PCa)is invariably associated with the aggressiveness and metastasis of this disease.New emerging evidence indicates that the epithelial-to-mesenchymal transition(EMT)may play pivotal roles in the development of chemoresistance and metastasis.As a hallmark of EMT,E-cadherin is suggested to be a key marker in the development of chemoresistance.However,the molecular mechanisms underlying PCa chemoresistance remain unclear.The current study aimed to explore the association between EMT and chemoresistance in PCa as well as whether changing the expression of E-cadherin would affect PCa chemoresistance.Methods:Parental PC3 and DU145 cells and their chemoresistant PC3-Tx R and DU145-Tx R cells were analyzed.PC3-Tx R and DU145-Tx R cells were transfected with E-cadherin-expressing lentivirus to overexpress E-cadherin;PC3 and DU145 cells were transfected with small interfering RNA to silence E-cadherin.Changes of EMT phenotype-related markers and signaling pathways were assessed by Western blotting and quantitative real-time polymerase chain reaction.Tumor cell migration,invasion,and colony formation were then evaluated by wound healing,transwell,and colony formation assays,respectively.The drug sensitivity was evaluated using MTS assay.Results:Chemoresistant PC3-Tx R and DU145-Tx R cells exhibited an invasive and metastatic phenotype that associated with EMT,including the down-regulation of E-cadherin and up-regulation of Vimentin,Snail,and N-cadherin,comparing with that of parental PC3 and DU145 cells.When E-cadherin was overexpressed in PC3-Tx R and DU145-Tx R cells,the expression of Vimentin and Claudin-1 was down-regulated,and tumor cell migration and invasion were inhibited.In particular,the sensitivity to paclitaxel was reactivated in E-cadherin-overexpressing PC3-Tx R and DU145-Tx R cells.When E-cadherin expression was silenced in parental PC3 and DU145 cells,the expression of Vimentin and Snail was up-regulated,and,particularly,the sensitivity to paclitaxel was decreased.Interestingly,Notch-1 expression was up-regulated in PC3-Tx R and DU145-Tx R cells,whereas the E-cadherin expression was down-regulated in these cells comparing with their parental cells.The use ofγ-secretase inhibitor,a Notch signaling pathway inhibitor,significantly increased the sensitivity of chemoresistant cells to paclitaxel.Conclusion:The down-regulation of E-cadherin enhances PCa chemoresistance via Notch signaling,and inhibiting the Notch signaling pathway may reverse PCa chemoresistance. | Wenchu Wang Lihui Wang Atsushi Mizokami Junlin Shi Chunlin Zou Jinlu Dai Evan T. Keller Yi Lu Jian Zhang | 2017 | Chinese Journal of Cancer2017，36，3： | 16 |
| 4 | Issues and solutions toward zinc anode in aqueous zinc-ion batteries: A mini review显示文摘Aqueous zinc-ion batteries(ZIBs)have been intensively investigated as potential energy storage devices on account of their low cost,environmental benignity,and intrinsically safe merits.With the exploitation of highperformance cathode materials,electrolyte systems,and in-depth mechanism investigation,the electrochemical performances of ZIBs have been greatly enhanced.However,there are still some challenges that need to be overcome before its commercialization.Among them,the obstinate dendrites,corrosion,and hydrogen evolution reaction(HER)on Zn anodes are critical issues that severely limit the practical applications of ZIBs.To address these issues,various strategies have been proposed,and tremendous progress has been achieved in the past few years.In this article,we analyze the origins and effects of the dendrites,corrosion,and HER on Zn anodes in neutral and mildly acid aqueous solutions at first.And then,a scientific understanding of the fundamental design principles and strategies to suppress these problems are emphasized.Apart from these,this article also puts forward some requirements for the practical applications of Zn anodes as well as several cost-effectivemodifying strategies.Finally,perspectives on the future development of Zn anodes in aqueous solutions are also briefly anticipated.This article provides pertinent insights into the challenges on anodes for the development of highperformance ZIBs,which will greatly contribute to their practical applications. | Chunlin Xie Yihu Li Qi Wang Dan Sun Yougen Tang Haiyan Wang | 2020 | Carbon Energy2020，2，4： | 15 |
| 5 | Neural Networks Combined with Importance Sampling Techniques for Reliability Evaluation of Explosive Initiating Device显示文摘Concerning the issue of high-dimensions and low-failure probabilities including implicit and highly nonlinear limit state function, reliability analysis based on the directional importance sampling in combination with the radial basis function (RBF) neural network is used, and the RBF neural network based on first-order reliability method (FORM) is to approximate the unknown implicit limit state functions and calculate the most probable point (MPP) with iterative algorithm. For good efficiency, based on the ideas that directional sampling reduces dimensionality and importance sampling focuses on the domain contributing to failure probability, the joint probability density function of importance sampling is constructed, and the sampling center is moved to MPP to ensure that more random sample points draw belong to the failure domain and the simulation efficiency is improved. Then the numerical example of initiating explosive devices for rocket booster explosive bolts demonstrates the applicability, versatility and accuracy of the approach compared with other reliability simulation algorithm. | GONG Qi ZHANG Jianguo TAN Chunlin WANG Cancan | 2012 | Chinese Journal of Aeronautics2012，25，2： | 10 |
| 6 | Enhanced efficiency of cadmium removal by Boehmeria nivea(L.) Gaud. in the presence of exogenous citric and oxalic acids显示文摘Boehmeria nivea(L.)Gaud.is a potential candidate for the remediation of Cd contaminated sites.The present investigation aims to explore Cd tolerance threshold and to quickly identify the role of exogenous organic acids in Cd uptake and abiotic metal stress damage.Elevated Cd levels(0–10 mg/L)resulted in an obvious rise in Cd accumulation,ranging from268.0 to 374.4 in root and 25.2 to 41.2 mg/kg dry weight in shoot,respectively.Citric acid at1.5 mmol/L significantly facilitated Cd uptake by 26.7%in root and by 1-fold in shoot,respectively.Cd translocation efficiency from root to shoot was improved by a maximum of66.4%under 3 mmol/L of oxalic acid.Citric acid exhibited more prominent mitigating effect than oxalic acid due to its stronger ligand affinity for chelating with metal and avoiding the toxicity injury of free Cd ions more efficiently.The present work provides a potential strategy for efficient Cd remediation with B.nivea. | Huaying Li Yunguo Liu Guangming Zeng Lu Zhou Xin Wang Yaqin Wang Chunlin Wang Xinjiang Hu Weihua Xu | 2014 | Journal of Environmental Sciences2014，26，12： | 10 |
| 7 | Linkage between permafrost distribution and river runoff changes across the Arctic and the Tibetan Plateau显示文摘River runoff in the Arctic and the Tibetan Plateau(TP) change significantly in recent decades. However, the mechanisms of the physical processes of permafrost river runoff change remain uncertain across large scale. This study investigated the mainstreams and tributaries of main Arctic and TP rivers dominated by permafrost and assessed the linkage between hydrological regime change and permafrost. The results show that the effects of permafrost on river runoff are highly dependent on the permafrost coverage of a watershed. For the past decades, the majority of the Arctic and TP basins showed increased discharge, while all of the studied basins showed increased baseflow, with faster increasing speed than total discharge.Both total discharge and baseflow annual change rate(ΔQ and ΔBF) increased with permafrost coverage, indicating the increments of streamflow are enhanced with high permafrost coverage. Meanwhile, the annual change of precipitation showed weak connection with total discharge and baseflow change. The high permafrost coverage basins showed high annual maximum/minimum discharge ratio(Qmax/Qmin), while the Qmax/Qminchanged slightly in low permafrost cover basins. Our results highlight the importance of permafrost coverage on streamflow regime change for permafrost basins across the northern hemisphere. Due to these linkage between permafrost extent and runoff regime change and the increasing changes of permafrost, more attention should be paid to the change of hydrological processes in permafrost-underlain basins. | Chunlin SONG Genxu WANG Tianxu MAO Junchen DAI Daqing YANG | 2020 | Science China Earth Sciences2020，63，2： | 10 |
| 8 | Deformation Stages and Ar-Ar Age Data of the Wan-Zhe-Gan Tectonic Zone,Southeast China,and Their Tectonic Significance显示文摘在东南中国通过安徽,浙江,和江西省的边阶区域的主要构造地区通常被叫作 Wan-Zhe-Gan 差错地区。地质上,这个地区由各种各样的年龄和取向的几条地区性的差错带组成。我们基于地调查把差错分成了四个年龄组。Neoproterozoic 差错向东北正在罢工。他们从东北江西省开始并且延长对在安徽省的 Fuchuan 向东北,东北 Jiangxi-Fuchuan ophiolite 带的一样的地点。可能作为一条边界在 Neoproterozoic 期间扮演的差错与 m 指责一个盘子或块缝术的地区 ???? | YU Xinqi WANG De'en JIANG Dazhi JIANG Laili ZHOU Xiang LI Chunlin | 2011 | Acta Geologica Sinica(English Edition)2011，85，6： | 10 |
| 9 | Labeling of cynomolgus monkey bone marrow-derived mesenchymal stem cells for cell tracking by multimodality imaging显示文摘Recently,transplantation of allogeneic and autologous cells has been used for regenerative medicine.A critical issue is monitoring migration and homing of transplanted cells,as well as engraftment efficiency and functional capability in vivo.Monitoring of superparamagnetic iron oxide(SPIO) particles by magnetic resonance imaging(MRI) has been used in animal models and clinical settings to track labeled cells.A major limitation of MRI is that the signals do not show biological characteristics of transplanted cells in vivo.Bone marrow mesenchymal stem cells(MSCs) have been extensively investigated for their various therapeutic properties,and exhibit the potential to differentiate into cells of diverse lineages.In this study,cynomolgus monkey MSCs(cMSCs) were labeled with Molday ION Rhodamine-BTM(MIRB),a new SPIO agent,to investigate and characterize the biophysical and MRI properties of labeled cMSCs in vitro and in vivo.The results indicate that MIRB is biocompatible and useful for cMSCs labeling and cell tracking by multimodality imaging.Our method is helpful for detection of transplanted stem cells in vivo,which is required for understanding mechanisms of cell therapy. | REN ZhenHua WANG JiaYin ZOU ChunLin GUAN YunQian ZHANG Yu Alex | 2011 | Science China(Life Sciences)2011，54，11： | 9 |
| 10 | 2,5-Furandicarboxylic acid production via catalytic oxidation of 5-hydroxymethylfurfural:Catalysts,processes and reaction mechanism显示文摘Biomass conversion to value-added chemicals has received tremendous attention for solving global warming issues and fossil fuel depletion.5-Hydroxymethylfurfural(HMF)is a key bio-based platform molecule to produce many useful organic chemicals by oxidation,hydrogenation,polymerization,and ring-opening reactions.Among all derivatives,the oxidation product 2,5-furandicarboxylic acid(FDCA)is a promising alternative to petroleum-based terephthalic acid for the synthesis of biodegradable plastics.This review analytically discusses the recent progress in the thermocatalytic,electrocatalytic,and photocatalytic oxidation of HMF into FDCA,including catalyst screening,synthesis processes,and reaction mechanism.Rapid fundamental advances may be possible in non-precious metal and metal-free catalysts that are highly efficient under the base-free conditions,and external field-assisted processes like electrochemical or photoelectrochemical cells. | Chunlin Chen Lingchen Wang Bin Zhu Zhenqiang Zhou Soliman I.El-Hout Jie Yang Jian Zhang | 2021 | Journal of Energy Chemistry2021，30，3： | 9 |
| 11 | Phylogenetic and genetic characterization of a 2017 clinical isolate of H7N9 virus in Guangzhou,China during the fifth epidemic wave显示文摘Pathogenic H7N9 influenza viruses continue to pose a public health concern. The H7N9 virus has caused five outbreak waves of human infections in China since 2013. In the present study, a novel H7N9 strain(A/Guangdong/8 H324/2017) was isolated from a female patient with severe respiratory illness during the fifth wave of the 2017 H7N9 epidemic. Phylogenetic analysis showed that the H7N9 viruses collected during the fifth wave belong to two different lineages: the Pearl River Delta lineage and the Yangtze River Delta lineage. The novel isolate is closely related to the Pearl River Delta H7N9 viruses, which were isolated from patients in Guangdong Province. The novel H7N9 isolate has an insertion of three basic amino acids in the cleavage site of hemagglutinin(HA), which may enhance virulence in poultry. The 2017 isolate also possesses an R292 K substitution in the neuraminidase(NA)protein, which confers oseltamivir resistance. This study highlights the pandemic potential of the novel H7N9 virus in mammals;thus, future characterization and surveillance is warranted. | Yongqiang Deng Chunlin Li Jianfeng Han Yingfen Wen Jian Wang Wenxing Hong Xiaofeng Li Zhongyu Liu Qing Ye Jing Li Changshuai Zhou Lei Yu Chengfeng Qin Fuchun Zhang Tao Jiang | 2017 | Science China(Life Sciences)2017，60，12： | 9 |
| 12 | Speciation analysis of metals (Tl,Cd and Pb) in Tl-containing pyrite and its cinder from Yunfu Mine,China,by ICP-MS with sequential extraction显示文摘The speciation of heavy metals such as thallium,cadmium and lead existing in pyrite and pyrite cinder was analyzed by ICP-MS with a sequential extraction procedure.The distribution patterns of these metals including exchangeable,reducible,oxidizable and residual fractions were obtained.Tl,Cd and Pb in pyrite and pyrite cinder samples from each extraction step were determined by inductively coupled plasma mass spectrometry(ICP-MS).Under the optimized instrumental conditions,detection limits of Tl,Cd and Pb in different matrices were within the range of 0.006-0.07 μg/L,and the relative standard deviations ranged from 0.8% to 1.2%.The accuracy of Tl,Cd and Pb determination was checked by analyzing two certified reference materials.The results demonstrate that trace Tl,Cd and Pb in the samples can be accurately determined.The sequential extraction results revealed that the percent contents of Tl,Cd and Pb in exchangeable,reducible and oxidizable fractions in pyrite cinder are different from those in pyrite and in pyrite cinder.Tl,Cd and Pb mostly are distributed in residual fraction.Therefore,the mobility of metals in pyrite is higher than that in pyrite cinder.Although distributions of Tl,Cd and Pb in the non-residual fraction are not dominant in pyrite cinder,the total concentrations of them could not be ignored.Consequently,attention must be paid to the risk of potential pollution by pyrite cinder. | WANG Chunlin CHEN Yongheng PAN Jianyang ZHANG Ping QI Jianying LIU Juan LI Xiangping WANG Jin | 2010 | Chinese Journal Of Geochemistry2010，29，1： | 9 |
| 13 | A New Species of Phoenicopsis Subgenus Windwardia(Florin) Samylina(Czekanowskiales) from the Middle Jurassic of Inner Mongolia, China显示文摘A new species of Phoenicopsis subgenus Windwardia(Florin) Samylina, Phoenicopsis neimengguensis sp.nov., is recognized from the Middle Jurassic Zhaogou Formation in the Shiguai Basin, a Jurassic coal-bearing sedimentary basin in Inner Mongolia, China. Linear leaves are in bundles of six to eight and are attached on a short shoot. The leaf is amphistomatic with one or two irregular rows of longitudinally oriented stomata in each intercostal zone on the upper epidermis and three or four(occasionally six) rows of stomata in each intercostal zone on the lower epidermis. Each stomata is surrounded by four to six subsidiary cells with unevenly developed papillae. The anticlinal walls of epidermal cells are straight. Cuticular thickenings or papillae are present on the outer surface of the periclinal walls. This is the first time that Phoenicopsis subgenus Windwardia(Florin) Samylina is recognized from the Middle Jurassic of Inner Mongolia, China. The occurrence of the new species is noteworthy because it extends the geological and geographic distribution of Phoenicopsis subg. Windwardia in China and Eurasia. The discovery is significant as it increases our knowledge of Phoenicopsis subg. Windwardia by providing new data on morphological and epidermal features. | SUN Chunlin NA Yuling David L.DILCHER WANG Hongshan LI Tao LI Yunfeng | 2015 | Acta Geologica Sinica(English Edition)2015，89，1： | 7 |
| 14 | Grey systems for intelligent sensors and information processing显示文摘In a measurement system,new representation methods are necessary to maintain the uncertainty and to supply more powerful ability for reasoning and transformation between numerical system and symbolic system.A grey measurement system is discussed from the point of view of intelligent sensors and incomplete information processing compared with a numerical and symbolized measurement system.The methods of grey representation and information processing are proposed for data collection and reasoning.As a case study,multi-ultrasonic sensor systems are demonstrated to verify the effectiveness of the proposed methods. | Chen Chunlin Dong Daoyi Chen Zonghai Wang Haibo | 2008 | Journal of Systems Engineering and Electronics2008，19，4： | 7 |
| 15 | Pore throat characteristics of tight sandstone of Yanchang Formation in eastern Gansu,Ordos Basin显示文摘An important factor to evaluate reservoir quality is the pore-throat size.However,the strong heterogeneity makes it difficult to characterize the pore-throat distribution in tight reservoirs.The field emission scanning electron microscope(FESEM),high pressure mercury injection and rate-controlled mercury injection are used to investigate the pore-throat size distribution in tight sandstone reservoirs of Member 7 of the Yanchang Formation in eastern Gansu,Ordos Basin,and studies of the pore throat size controlling on physical property of the tight sandstone reservoirs are also carried out.The result shows that the pore type is mainly dominated by the residual intergranular pore,dissolution pore,micropore and a few micro-fractures;the high-pressure mercury injection experiment indicates that the pore-throat size ranges from 0.0148 μm to 40mm,the pore throat more than 1 mm is less;the ratecontrolled mercury injection experiment reveals that for samples with different physical properties,the pore radius mainly varies from 80 μm to 350 μm;the throat radius exhibits the strong heterogeneity,and is from 0.12 μm to 30μm;the pore-throat size can be effectively characterized by combination of high-pressure and rate-controlled mercury injections,and it varies from 0.0148 μm to 350 μm.The permeability is mainly controlled by the large pore throat(>R_(50))which accounts for a small proportion;in the tight sandstone with the permeability greater than 0.1 mD,the permeability is mainly controlled by the micropore and mesopore;in the tight sandstone with the permeability smaller than 0.1 mD,the permeability is mainly controlled by the nanopore and micropore;the proportion of small pore throat increases with reduction of permeability,it is important that the small pore throat influences the reservoir storage property though its effect on permeability are small. | Hao Wu Chunlin Zhang Youliang Ji Rui'e Liu Shang Cao Sheng Chen Yunzhao Zhang Ye Wang Wei Du Gang Liu | 2018 | Petroleum Research2018，3，1： | 7 |
| 16 | Nanometer lapping technology at high speed显示文摘In floating lapping with solid abrasives, the workpiece is taken as an isolated body. The forces that act on it are analyzed. A differential equation about the forces that act on it is set up, so the forces that act on it and its motion rule are received. Combining it with known lapping tool motion, the relative motion rule between the lapping tool and workpiece is determined too. According to the relative motion, the distribution of abrasives density is designed reasonably, which makes the lapping tool wear uniformly, which, in turn, avoids redressing the lapping tool, saves abra-sives, and increases machining accuracy. Combining it with advantages in high speed lapping with solid abrasives, the low cost, high efficiency nanometer lapping at high speed is realized. | YANG JianDong TIAN ChunLin WANG ChangXing | 2007 | Science China(Technological Sciences)2007，50，1： | 6 |
| 17 | Early hyperbaric oxygen therapy inhibits aquaporin 4 and adrenocorticotropic hormone expression in the pituitary gland of rabbits with blast-induced craniocerebral injury显示文摘In the present study, rabbits were treated with hyperbaric oxygen for 1 hour after detonator-blast-induced craniocerebral injury. Immunohistochemistry showed significantly reduced aquaporin 4 expression and adrenocorticotropic hormone expression in the pituitary gland of rabbits with craniocerebral injury. Aquaporin 4 expression was positively correlated with adrenocorticotropic hormone expression. These findings indicate that early hyperbaric oxygen therapy may suppress adrenocorticotropic hormone secretion by inhibiting aquaporin 4 expression. | Jian Huo Jiachuan Liu Jinbiao Wang Yongming Zhang Chunlin Wang Yanyan Yang WenjiangSun Shaonian Xu | 2012 | Neural Regeneration Research2012，7，22： | 6 |
| 18 | Effects of fluoxetine on protein expression of potassium ion channels in the brain of chronic mild stress rats显示文摘The purpose of this study is to investigate the expression of major potassium channel subtypes in the brain of chronical mild stress(CMS) rats and reveal the effects of fluoxetine on the expression of these channels. Rats were exposed to a variety of unpredictable stress for three weeks and induced anhedonia, lower sucrose preference, locomotor activity and lower body weight. The protein expressions were determined by Western blot. CMS significantly increased the expression of Kv2.1 channel in frontal cortex but not in hippocampus, and the expression level was normalized after fluoxetine treatment. The expression of TREK-1 channel was also obviously increased in frontal cortex in CMS rats. Fluoxetine treatment might prevent this increase. However, the expression of Kv3.1 and Kv4.2 channels was considerably decreased in hippocampus after CMS, and was not affected by fluoxetine. These results suggest that different subtypes of potassium channels are associated with the pathophysiology of depression and that the therapeutical effects of fluoxetine may relate to Kv2.1 and TREK-1 potassium channels. | Chunlin Chen Ling Wang Xianfang Rong Weiping Wang Xiaoliang Wang | 2015 | Acta Pharmaceutica Sinica B2015，5，1： | 6 |
| 19 | Inducible regulation of GDNF expression in human neural stem cells显示文摘Glial cell derived neurotrophic factor (GDNF) holds promises for treating neurodegenerative diseases such as Parkinson's disease. Human neural stem cells (hNSCs) have proved to be a suitable cell delivery vehicle for the safe and efficient introduction of GDNF into the brain. In this study, we used hNSCs-infected with a lentivirus encoding GDNF and the hygromycin resistance gene as such vehicles. A modified tetracycline operator 7 (tetO7) was inserted into a region upstream of the EF1- promoter to drive GDNF expression. After hygromycin selection, hNSCs were infected with a lentivirus encoding a KRAB-tetracycline repressor fusion protein (TTS). TTS bound to tetO7 and suppressed the expression of GDNF in hNSCs. Upon administration of doxycycline (Dox) the TTS-tetO7 complex separated and the expression of GDNF resumed. The hNSCs infected with GDNF expressed the neural stem cell specific markers, nestin and sox2, and exhibited no significant change in proliferation rate. However, the rate of apoptosis in hNSCs expressing GDNF was lower compared with normal NSCs in response to actinomycin treatment. Furthermore, a higher percentage of Tuj-1 positive cells were obtained from GDNF-producing NSCs under conditions that induced differentiation compared to control NSCs. The inducible expression of GDNF in hNSCs may provide a system for the controllable delivery of GDNF in patients with neurodegenerative diseases. | WANG ShuYan REN Ping GUAN YunQian ZOU ChunLin FU LinLin ZHANG Yu | 2013 | Science China(Life Sciences)2013，56，1： | 5 |
| 20 | The PL 'violet shift' of cerium dioxide on silicon显示文摘CeO2 thin film was fabricated by dual ion beam epitaxial technique. The phenomenon of PL violet shift at room temperature was observed, and the distance of shift was about 65 nm. After the analysis of crystal structure and valence in the compound were carried out by XRD and XPS technique, it was concluded that the PL shift was related with valence of cerium ion in the oxides. When the valence of cerium ion varied from tetravalence to trivalence, the PL peak position would move from blue region to violet region and the phenomenon of 'violet shift' was observed. | Chai Chunlin Yang Shaoyan Liu Zhikai Liao Meiyong Chen Nuofu Wang Zhanguo | 2001 | Chinese Science Bulletin2001，46，24： | 5 |
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