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| 1 | 二氢嘧啶脱氢酶、胸苷酸合成酶、c-myc和p53基因在小鼠肝组织中表达的昼夜节律性研究(英文)显示文摘背景与目的:5-Fluorouracil(5-FU)主要通过抑制胸苷酸合成酶发挥抗癌作用,而二氢嘧啶脱氢酶是5-FU体内代谢的一个起始酶和限速酶。在本研究中,我们对二氢嘧啶脱氢酶、胸苷酸合成酶、c-myc和p53基因在小鼠肝脏中表达的昼夜节律进行研究。方法:本实验共采用了24只雄性B6D2F1小鼠。所有实验动物在实验中进行了4周光照同步化(12h光照:12h黑暗)。体温和休息-活动节律通过腹腔埋置的微型感受器进行动态监测。动物分别在3,7,11,15,19,23HALO(灯亮后小时数)被处死并取出肝脏冻存。肝脏标本中抽提的总RNA用LightCycler-SYBRGreenITM扩增系统进行实时定量RT-PCR。结果:小鼠的体温和休息-活动均呈现明显和相似的昼夜节律,其峰值均位于黑暗期(相当于小鼠活动相),谷值则位于光照期(相当于小鼠休息相)。二氢嘧啶脱氢酶在mRNA水平呈现出明显的昼夜节律性,其峰值时间位于16HALO左右即位于小鼠活动相(P=0.0012)。胸苷酸合成酶在mRNA水平具有昼夜节律表达的趋势,其峰值时间出现在光照期(P=0.079)。C-myc和p53在mRNA水平的节律性表达在本实验中无统计学意义。结论:二氢嘧啶脱氢酶和胸苷酸合成酶在mRNA水平呈现出昼夜节律性,二者峰值时间相差约12h,这一结果提示可以利用二者在转录水平的时间差异用5-FU进行时间化疗。 | 吴明玮 冼励坚 李晓梅 lnnominato Pasquale lvi Francis | 2004 | 癌症2004，23，3： | 22 |
| 2 | 移植于裸鼠的人鼻咽癌细胞DNA合成生物节律的初步研究显示文摘目的:探索鼻咽癌细胞DNA合成的生物节律为临床制订鼻咽癌时辰化疗方案提供必要的参数。方法:BALB/C裸小鼠15只,置于程控的独立光照系统中(12h光照,12h黑暗)中同步化至少3周。然后双侧腋下接种人鼻咽低分化鳞癌细胞裸小鼠移植瘤CNE2,成瘤后按灯亮后3、9、15、21h(即3、9、15、21HALO)的时间点取瘤块,制成单个细胞悬液固定,染色后流式细胞仪测DNA含量。用SPSS软件系统ANOVA法检验各期细胞在4个时间点差异的显著性,用Cosinor法考察G1、S、G2/M期细胞在24h的分布是否符合余弦函数。结果:G1、S、G2/M期细胞在3、9、15、21HALO的分布随时间变化有显著性差异,G1、G2/M期细胞变化符合余弦节律。结论:移植于裸鼠的人鼻咽癌细胞的DNA合成随昼夜交替呈节律性变化。 | 孙健 冼励坚 曹弃元 叶燕丽 LIU Xu-hui LI Xiao-mei Francis Lé vi | 2001 | 癌症2001，20，2： | 21 |
| 3 | 皱褶结构芯格构型的几何设计方法显示文摘夹层结构具有较高的抗弯刚度质量比 ,较好的吸音、隔音、隔热和耐疲劳性能 ,被广泛应用于航空航天领域。夹层结构的芯材是具有一定抗剪刚度的轻质材料。从理论上讲 ,可以制造出各种不同构造型式的芯材。但由于工艺方法以及制造成本的限制 ,目前结构上常用的是由金属或非金属材料制成的六角蜂窝芯材。本文介绍了将平面板材通过按一定有规律的线系进行皱褶 ,从而形成各种不同构造型式、不同几何尺寸的等高度、变高度或曲面构型等复杂形状的芯材 ,用以满足各种不同的结构和功能性要求。而且 ,这种方法具有很好的工艺性。 | 王志瑾 Khaliulin VI Skripkin E | 2002 | 南京航空航天大学学报2002，34，1： | 20 |
| 4 | Evaluating Conservation Effectiveness of Nature Reserves Established for Surrogate Species:Case of a Giant Panda Nature Reserve in Qinling Mountains,China显示文摘Many nature reserves are established to protect the habitat needs of particular endangered species of interest but their effectiveness for protecting other species is questionable.In this study,this effectiveness was evaluated in a nature reserve network located in the Qinling Mountains,Shaanxi Province,China.The network of reserves was established mainly for the conservation of the giant panda,a species considered as a surrogate for the conservation of many other endangered species in the region.The habitat suitability of nine protected species,including the giant panda,was modeled by using Maximum Entropy(MAXENT)and their spatial congruence was analyzed.Habitat suitability of these species was also overlapped with nature reserve boundaries and their management zones(i.e.,core,buffer and experimental zones).Results show that in general the habitat of the giant panda constitutes a reasonable surrogate of the habitat of other protected species,and giant panda reserves protect a relatively high proportion of the habitat of other protected species.Therefore,giant panda habitat conservation also allows the conservation of the habitat of other protected species in the region.However,a large area of suitable habitat was excluded from the nature reserve network.In addition,four species exhibited a low proportion of highly suitable habitat inside the core zones of nature reserves.It suggests that a high proportion of suitable habitat of protected species not targeted for conservation is located in the experimental and buffer zones,thus,is being affected by human activities.To increase their conservation effectiveness,nature reserves and their management zones need to be re-examined in order to include suitable habitat of more endangered species.The procedures described in this study can be easily implemented for the conservation of many endangered species not only in China but in many other parts of the world. | XU Weihua Andrés VIA QI Zengxiang OUYANG Zhiyun LIU Jianguo LIU Wei WAN Hui | 2014 | Chinese Geographical Science2014，24，1： | 12 |
| 5 | Serum leptin levels and insulin resistance are associated with gallstone disease in overweight subjects显示文摘AIM: To establish an association between the serum leptin levels and the development of gallstone disease (GD).METHODS: We carried out a non-matched case-controlled study in a university hospital in Mexico City. Two hundred and eighty-seven subjects were included: 97 cases with gallstones and 190 controls. Body mass index (BMI), fasting plasma leptin, insulin, serum lipid, and lipoprotein levels were measured. Insulin resistance was calculated by homeostasis model assessment (HOMA-IR). Unconditional logistic regression analysis (univariate and multivariate)stratified by BMI was used to calculate the risk of GD.RESULTS: The multivariate conditional regression analysis revealed a model for those patients with BMI <30. The selected variables in the model were HOMA-IR index with OR = 1.31, P= 0.02 and leptin higher than median with OR = 2.11, P= 0.05. In the stratum of BMI ≥30, we did not find a useful model.CONCLUSION: We concluded that insulin resistance and the development of GD appears to be associated with serum leptin levels in subjects with overweight, but not in obese subjects with similar metabolic profiles. | Nahum Méndez-Sánchez Luisa B Bermejo-Martínez Yolanda Vi(n|~)als Norberto C Chavez-Tapia Irina Vander Graff Guadalupe Ponciano-Rodríguez Martha H Ramos Misael Uribe | 2005 | World Journal of Gastroenterology2005，11，39： | 11 |
| 6 | 肿瘤的时辰治疗显示文摘在实验动物中,30多种抗癌药物的细胞毒作用24小时中随给药时间不同而变化超过50%.其中的机制涉及细胞代谢,增殖过程以及药物的药动学的时间节律变化而且,在实验肿瘤模型中在最小毒性时间进行化疗通常可以达到最大的抗瘤效果. | Francis Lévi 孙健 冼励坚 | 2001 | 广州医药2001，32，2： | 6 |
| 7 | 电镀污水净化新工艺——电浮选方法简介显示文摘综述了电浮选方法处理电镀污水的基本原理和提高电浮选净水技术的途径。重点介绍了俄罗斯门捷列夫化工大学的电浮选两种新工艺方法。 | 邵会波 S O Varaxin V A Kolesnikov VI Il’in | 2003 | 化学通报2003，66，12： | 6 |
| 8 | 巨峰和无核早红葡萄在越南谅山试种观察显示文摘[目的]观察巨峰、无核早红葡萄在越南谅山省的引种表现,为其在越南推广种植提供参考依据.[方法]2009年春从中国南宁引入巨峰、无核早红两个鲜食葡萄品种在越南谅山省试种,调查其枝蔓生长量、物候期、果实性状、产量等相关指标.[结果]至第1年抚育冬季,巨峰葡萄枝蔓总长234.5 cm,上架茎粗0.84 cm;无核早红葡萄枝蔓总长294.1 cm,上架茎粗0.86 cm.在物候期方面,巨峰葡萄从萌芽至果实成熟需141~151 d,表现中熟;无核早红从萌芽至果实成熟需142~151 d,未表现其应有的早熟特性.在果实性状方面,巨峰葡萄2010~2012年3年平均单果重8.9 g,种子数1.04粒,可溶性固形物(TSS)含量17.5%;无核早红葡萄2010~2012年3年平均单果重6.8 g,TSS含量13.9%.在产量方面,巨峰葡萄2010~2012年产量分别为13.69、17.50和16.24 t/ha;无核早红葡萄2010~2012年产量分别为10.81、16.02和20.92t/ha.[结论]巨峰葡萄在越南谅山省种植表现丰产稳产优质,具有较好的适栽性,可大面积推广种植;无核早红葡萄表现丰产稳产,但表现低糖低酸,极易裂果,着色困难,不宜在越南大面积发展. | 黄江流 PHAM Thi Thang 罗瑞鸿 NGUYN Thi Hà 吕荣华 LUONG Dǎng Ning CHU Vǎn DUòng NGUYN Manh Tòng 彭宏祥 Ly Ming Nguyet LU'O'NG i Thuy CHU Xun Tien VI Vǎn Dúc NGUYN Duy Dng Ly Dúc Khiêm 熊俏 | 2014 | 南方农业学报2014，45，7： | 5 |
| 9 | Effective plant discrimination based on the combination of local binary pattern operators and multiclass support vector machine methods显示文摘Accurate crop and weed discrimination plays a critical role in addressing the challenges of weed management in agriculture.The use of herbicides is currently the most common approach to weed control.However,herbicide resistant plants have long been recognised as a major concern due to the excessive use of herbicides.Effective weed detection techniques can reduce the cost of weed management and improve crop quality and yield.A computationally efficient and robust plant classification algorithm is developed and applied to the classification of three crops:Brassica napus(canola),Zea mays(maize/corn),and radish.The developed algorithm is based on the combination of Local Binary Pattern(LBP)operators,for the extraction of crop leaf textural features and Support vector machine(SVM)method,for multiclass plant classification.This paper presents the first investigation of the accuracy of the combined LBP algorithms,trained using a large dataset of canola,radish and barley leaf images captured by a testing facility under simulated field conditions.The dataset has four subclasses,background,canola,corn,and radish,with 24,000 images used for training and 6000 images,for validation.The dataset is referred herein as 'bccr-segset'and published online.In each subclass,plant images are collected at four crop growth stages.Experimentally,the algorithm demonstrates plant classification accuracy as high as 91.85%,for the four classes. | Vi Nguyen Thanh Le Beniamin Apopei Kamal Alameh | 2019 | Information Processing in Agriculture2019，6，1： | 4 |
| 10 | Functional relationship between ammonia-oxidizing bacteria and ammonia-oxidizing archaea populations in the secondary treatment system of a full-scale municipal wastewater treatment plant显示文摘The abundance of ammonia-oxidizing bacteria and archaea and their amo A genes from the aerobic activated sludge tanks,recycled sludge and anaerobic digesters of a full-scale wastewater treatment plant(WWTP)was determined.Polymerase chain reaction and denaturing gradient gel electrophoresis were used to generate diversity profiles,which showed that each population had a consistent profile although the abundance of individual members varied.In the aerobic tanks,the ammonia-oxidizing bacterial(AOB)population was more than 350 times more abundant than the ammonia-oxidizing archaeal(AOA)population,however in the digesters,the AOA population was more than 10 times more abundant.Measuring the activity of the amo A gene expression of the two populations using RT-PCR also showed that the AOA amo A gene was more active in the digesters than in the activated sludge tanks.Using batch reactors and dd PCR,amo A activity could be measured and it was found that when the AOB amo A activity was inhibited in the anoxic reactors,the expression of the AOA amo A gene increased fourfold.This suggests that these two populations may have a cooperative relationship for the oxidation of ammonia. | Golam MIslam Peter Vi Kimberley Ann Gilbride | 2019 | Journal of Environmental Sciences2019，31，12： | 4 |
| 11 | 沿哥伦比亚—厄瓜多尔边界毁林速度和格局变化的卫星检测分析显示文摘本研究使用Landsat卫星数据来论证哥伦比亚—厄瓜多尔部分边界23年间(1973~1996)土地覆被变化的速度和格局。人类定居给这两个国家带来了大量毁林。变化的卫星检测分析表明,边界哥伦比亚一侧年毁林速度相对高得多。另外,哥伦比亚一侧森林覆被在研究期间几乎损失了43%,而在厄瓜多尔一侧只损失了22%。研究发现,在边界的任何一侧,都不存在单一的毁林驱动因素;但得出结论:哥伦比亚一侧的毁林速度高可能是由于定居压力高、非法古柯种植加剧。卫星图像证明,在厄瓜多尔一侧,毁林格局反映出与石油勘探和开发有关的道路网络。 | Andrés Via Fernando R.Echavarria Donald C.Rundquist 王胜 | 2004 | AMBIO-人类环境杂志2004，33，3： | 3 |
| 12 | Endoscopic ultrasound elastography strain histograms in the evaluation of patients with pancreatic masses显示文摘AIM: To investigate the accuracy of the strain histogram endoscopic ultrasound(EUS)-based method for the diagnostic differentiation of patients with pancreatic masses. METHODS: In a prospective single center study, 149 patients were analyzed, 105 with pancreatic masses and 44 controls. Elastography images were recorded using commercially available ultrasound equipment in combination with EUS linear probes. Strain histograms(SHs) were calculated by machine integrated software in regions of interest and mean values of the strain histograms were expressed as Mode 1(over the mass) and Mode 2(over an adjacent part of pancreatic tissue, representing the reference area). The ratio between Mode 2 and Mode 1 was calculated later, representing a new variable, the strain histogram ratio. After the final diagnosis was established, two groups of patients were formed: a pancreatic cancer group with positive cytology achieved by fine needle aspiration puncture or histology after surgery(58 patients), and a massforming pancreatitis group with negative cytology and follow-up after 3 and 6 mo(47 patients). All statistical analyses were conducted in SPSS 14.0(SPSS Inc., Chicago, IL, United States).RESULTS: Results were obtained with software for strain histograms with reversed hue scale(0 represents the hardest tissue structure and 255 the softest). Based on the receiver operating characteristics(ROC) curve coordinates, the cut-off point for Mode 1 was set at the value of 86. Values under the cut-off point indicated the presence of pancreatic malignancy. Mode 1 reached 100% sensitivity and 45% specificity with overall accuracy of 66%(95%CI: 61%-66%) in detection of pancreatic malignant tumors among the patients with pancreatic masses. The positive and negative predictive values were 54% and 100%, respectively. The cut-off for the new calculated variable, the SH ratio, was set at the value 1.153 based on the ROC curve coordinates. Values equal or above the cut-off value were indicative of pancreatic malignancy. The SH ratio reached 98% sensitivity, 50% specificity and an overall accuracy of 69%(95%CI: 63%-70%). The positive and negative predictive values were 92% and 100%, respectively.CONCLUSION: SH showed high sensitivity in pancreatic malignant tumor detection but disappointingly low specificity. Slight improvements in specificity and accuracy were achieved using the SH ratio. | Dalibor Opa?i? Nadan Rustemovi? Mirjana Kalauz Pave Marko? Zvonimir Ostoji? Matea Majerovi? Iva Ledinsky Ana Vi?nji? Juraj Krznari? Milorad Opa?i? | 2015 | World Journal of Gastroenterology2015，21，13： | 3 |
| 13 | Microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishments显示文摘 | M. Abadias J. Usall M. Anguera C. Solsona I. Vi?as | 2008 | International Journal of Food Microbiology2008，，1： | 2 |
| 14 | 矿石建造分析的新方法:以苏联东北部金矿床为例显示文摘 | 雅科.,VI 林强 | 1989 | 国外地质科技1989，，1： | 2 |
| 15 | Resection of Nonresectable Liver Metastases from Colorectal Cancer After Neoadjuvant Chemotherapy显示文摘 | Henry Bismuth René Adam Fran?is Lévi Christian Farabos Fabio Waechter Denis Castaing Pietro Majno Luc Engerran | 1996 | Annals of Surgery1996，，4： | 2 |
| 16 | Effect of low level laser therapy on dental pulp during orthodontic movement显示文摘AIM: To validate the protocol described here to be used in future clinical trials related to the effect of laser therapy on dental pulp. METHODS: Histologically treated samples from eight human healthy premolar teeth obtained from the middle root level were distributed in four groups: group 1(G1) absolute control; group 2(G2) only laser irradiation; group 3(G3) exposed only to orthodontics; and group 4(G4) treated with orthodontics and laser. Laser treatment was performed at 830 nm wavelength, 100 mW(energy 80 J/cm2, 2.2 J), for 22 s in the vestibular surface and 22 s in the palatal surface, 1 mm away from the dental root mucosa. Three staining methods were performed: hematoxylin-eosin(HE), Masson's Trichrome method and Gomori's method.RESULTS: The pulp histology parameters were evaluated and the results classified in to 3 parts: an inflammatory response, soft tissue response(dental pulp) and hard tissue response(dentin and predentin). There was no inflammation(chronic or acute) in any of the evaluated groups. The zones of pulp necrosis were found in one premolar of G3 and in one of G4; in groups G2 and G4 there was higher angiogenesis than in the other two groups. G4 group presented the highest level of vascularization. A reduced nerve density was observed in G3. A G2 specimen showed increased nerve density. A higher rate of calcification was observed in G1 compared to G2. Denticles, either real or false, were observed in G1, G2 and G3. Sclerosis of dentin and focal dentin loss was observed among all the groups. Secondary dentin was present in one sample in G1 and G2. A necrosis zone was found in one sample of G3 and G4. No differences between groups were observed in the odontoblast irregularity layer but the layer was wider in the group treated with laser only. A notable difference was detected in reduction of the cell-free layer between the groups G1 and G4. The findings in pulp tissue favor its adaptative response against dental movement induced by orthodontics. No definitive conclusions may be derived as this is a pilot study. CONCLUSION: The protocol described here was shown to be an effective method to evaluate changes in dental pulp submitted to low level laser in teeth under orthodontic movement. | ngela Domínguez Rosa Emilia Ballesteros Jairo Hernán Viáfara Oscar Mario Tamayo | 2013 | World Journal of Methodology2013，3，2： | 2 |
| 17 | Tumor Marker Evolution: Comparison with Imaging for Assessment of Response to Chemotherapy in Patients with Colorectal Liver Metastases显示文摘 | Robbert J. Haas Dennis A. Wicherts Eduardo Flores Michel Ducreux Francis Lévi Bernard Paule Daniel Azoulay Denis Castaing Antoinette Lemoine René Adam | 2010 | Annals of Surgical Oncology2010，，4： | 2 |
| 18 | Laparoscopic myomectomy in treatment of large myomas 显示文摘 | ADAMIAN LV KULAKOV VI KISELEV SI | 1996 | J Am Asscc Gynecol Laparosc1996，3，4： | 1 |
| 19 | Treatment of melanomas of the anorectal region 显示文摘 | Knysh VI Timofeev IUM Serebriakova ES | 1987 | Vopr Onkol1987，33，3： | 1 |
| 20 | Potassium channel gene mutations rarely cause atrial fibrillation显示文摘 | Ellinor PT Petrov-Kibdrativ VI Zakharova E | 2006 | BMC Med Genel2006，7，： | 1 |
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