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| 1 | Correlation between rpoB gene mutation in Mycobacterium avium subspecies paratuberculosis and clinical rifabutin and rifampicin resistance for treatment of Crohn’s disease显示文摘AIM: To investigate overlapping regions of the rpoB gene previously involved with rifamycin resistance in M. tuberculosis and seek correlation between rpoB mutations in clinical MAP strains with susceptibility to RIF and RFB. METHODS: We designed a molecular-based PCR method for the evaluation of rifabutin (RFB) and rifampicin (RIF) resistance based on probable determinant regions within the rpoB gene of MAP, including the 81 bp variable site located between nucleotides 1363 and 1443. The minimum inhibitory concentration (MIC) for RIF was also determined against 11 MAP isolates in attempt to seek correlation with rpoB sequences. RESULTS: We determined that MAP strain 18 had an MIC of > 30 mg/L and ≤ 5 mg/L for RIF and RFB respectively, and a significant and novel rpoB mutation C1367T, compared to an MIC of ≤ 1.0 mg/L for both drugs in the wild type MAP. The 30-fold increase in the MIC was a direct result of the rpoB mutation C1367T, which caused an amino acid change Thr456 to Ile456 in the drug’s binding site. In addition, MAP strain 185 contained five silent rpoB mutations and exhibited an MIC comparable to the wild-type. Moreover, our in vitroselected mutation in MAP strain UCF5 resulted in the generation of a new resistant strain (UCF5-RIF16r) that possessed T1442C rpoB mutation and an MIC > 30 mg/L and > 10 mg/L for RIF and RFB respectively. Sequencing of the entire rpoB gene in MAP strains UCF4, 18, and UCF5-RIF16r revealed an rpoB mutation A2284C further downstream of the 81 bp variable region in UCF4, accounting for observed slight increase in MIC. In addition, no other significant mutations were found in strains 18 and UCF-RIF16r. CONCLUSION: The data clearly illustrates that clinical and in vitro-selected MAP mutants with rpoB mutations result in resistance to RIF and RFB, and that a single amino acid change in the beta subunit may have a significant impact on RIF resistance. Unconventional drug susceptibility testing such as our molecular approach will be beneficial for evaluation of antibiotic effectiveness. This molecular approach may also serve as a model for other drugs used for treatment of MAP infections. | Daniel R Beckler Sammer Elwasila George Ghobrial John F Valentine Saleh A Naser | 2008 | World Journal of Gastroenterology2008，14，17： | 2 |
| 2 | The effect of flutamide on the physical working capacity and activity of some of the key enzymes for the energy supply in adult rats显示文摘学习的目的是在老鼠为精力供应在一些关键肌肉酶的物理工作能力和活动上估计雄激素受体对手的效果。年轻成年男 Wistar 老鼠被划分成二个组。一个组一个星期和作为控制为服务 8 个星期的另外的组每天收到了 flutamide 的 15 mg kg-1 6 天。在开始并且在实验的结束,所有老鼠受到 submaximal 跑耐力(SRE ) ,疲劳(MTE ) 的最大的时间,和最大的疾跑速度(女士) 测试。在试用的结束,最大的氧消费(VO2max ) 测试被执行并且睾丸激素,红血球,血红素以及 succinate 脱氢酶(同步数字系列) 的酶活动的层次,喂奶脱氢酶(LDH ) ,和 gastrocnemius 肌肉的 NAD.H2-cytochrome-c reductase (NAD.H2 ) 被测量。对待 flutamide 的老鼠的浆液睾丸激素比控制的高,它验证选择的剂量的有效性。anti-androgen-treated 组的 MTE 和 SRE 与起始的价值相比是更低的。Flutamide 处理减少了同步数字系列和 NAD.H2 的活动与控制相比。我们没在女士, VO2max,跑的经济, LDH 活动,和 hematological 变量上发现反雄激素处理的效果。我们的调查结果显示象一些关键酶的活动一样的 submaximal 和最大的跑耐力的维护把氧化能力与肌肉联系了与雄激素效果被连接由雄激素受体调停了。 | Katerina N Georgieva Penka A Angelova Fani D Gerginska Dora D Terzieva Mihaela S Shishmanova-Doseva Slavi D Delchev Valentine V Vasilev | 2017 | Asian Journal of Andrology2017，19，4： | 2 |
| 3 | 泌乳猪饲粮中添加维生素A对仔猪肝脏维生素A水平的影响显示文摘背景:哺乳期母体摄入200000-400000IU的大剂量维生素A可提高母体和婴儿的维生素A水平。然而,其最适宜的添加方案尚不明确。目标:探讨哺乳母猪添加大剂量维生素A对哺乳仔猪肝脏维生素A浓度的影响。设计:每头泌乳猪视黄醇醋酸酯油剂胶丸的添加水平分别为:2.1mmol(高剂量组),1.05mmol(低剂量组),0(对照组);每个处理3只试猪。仔猪试验期为3d和14d,在随后的4d饲喂无维生素A的饲粮,然后屠宰。分析肝脏和血清中的维生素A浓度。 | Valentine A R Tanumihardjo S A | 2007 | 饲料博览(技术版)2007，，9： | 2 |
| 4 | Postoperative change of anti-Thomsen-Friedenreich and Tn IgG level: The follow-up study of gastrointestinal cancer patients显示文摘AIM: To study the influence of tumor removal on the serum level of IgG antibodies to tumor-associated Thomsen-Friedenreich (TF), Tn carbohydrate epitopes and xenogeneic αGal, and to elucidate on the change of the level during the follow-up as well as its association with the stage and morphology of the tumor and the values of blood parameters in gastrointestinal cancer. METHODS: Sixty patients with gastric cancer and 34 patients with colorectal cancer in stages Ⅰ-Ⅳ without distant metastases were subjected to follow- up. The level of antibodies in serum was determined by the enzyme-linked immunosorbent assay (ELISA) using synthetic polyacrylamide (PAA) glycoconjugates. Biochemical and haematological analyses were performed using automated equipment. RESULTS: In gastrointestinal cancer, the TF antibody level was found to have elevated significantly after the removal of G3 tumors as compared with the preoperative level (u = 278.5, P < 0.05). After surgery, the TF and Tn antibody level was elevated in the majority of gastric cancer patients (sign test, 20 vs 8, P < 0.05, and 21 vs 8, P < 0.05, respectively). In gastrointestinal cancer, the elevated postoperative level of TF, Tn and αGal antibodies was noted in most patients with G3 tumors (sign test, 22 vs 5, P < 0.01; 19 vs 6, P < 0.05; 24 vs 8, P < 0.01, respectively), but the elevation was not significant in patients with G1 + G2 resected tumors. The postoperative follow-up showed that the percentage of patients with G3 resected tumors of the digestive tract, who had a mean level of anti-TF IgG above the cut- off value (1.53), was significantly higher than that of patients with G1 + G2 resected tumors (χ2 = 3.89, all patients; χ2 = 5.34, patients without regional lymph node metastases; P < 0.05). The percentage of patients with a tumor in stage I, whose mean anti-TF IgG level remained above the cut-off value (1.26), was significantly higher than that of patients with the cancer in stages Ⅲ-Ⅳ (χ2 = 4.71, gastric cancer; χ2 = 4.11, gastrointestinal cancer; P < 0.05). The correlation was observed to exist between the level of anti-TF IgG and the count of lymphocytes (r = 0.517, P < 0.01), as well as between the level of anti- Tn IgG and that of serum CA 19-9 (r = 0.481, P < 0.05). No positive delayed-type hypersensitivity reaction in skin test challenges with TF-PAA in any of the fifteen patients, including those with a high level of anti-TF IgG, was observed. CONCLUSION: The surgical operation raises the level of anti-carbohydrate IgG in most patients, especially in those with the G3 tumor of the gastrointestinal tract. The follow-up demonstrates that after surgery the low preoperative level of TF antibodies may be considerably increased in patients with the carcinoma in its early stage but remains low in its terminal stages. The stage- and morphology-dependent immunosuppression affects the TF-antibody response and may be one of the reasons for unresponsiveness to the immunization with TF-antigens. | Eugeniy P Smorodin Oleg A Kurtenkov Boris L Sergeyev Kristel E Kodar Valentin I Chuzmarov Vladimir P Afanasyev | 2008 | World Journal of Gastroenterology2008，14，27： | 2 |
| 5 | Diagnosis of masses presenting within the venticles on computed tomography显示文摘 | Kendall B Reider G Valentine A | 1983 | Neuroradiology1983，25，1： | 1 |
| 6 | CFD Evaluation of Waterwall Wastage in Coal-fired Utility Boilers 显示文摘 | VALENTINE J R SHIM H-S DAVIS K A | 2007 | Energy & Fuels2007，21，： | 1 |
| 7 | Diagnosis of masses presenting within the ventricules on computed tomography显示文摘 | Kendall B Reider G Valentine A | 1983 | Neuroradiology1983，25，： | 1 |
| 8 | Receptor technology-cell binding to P-15:a new method of regenerating bone quickly and safely-preliminary histomorphometrical and mechanical results in sinus floor augmentations显示文摘 | VALENTIN A H WEBER J | 2004 | Keio J Med2004，53，3： | 1 |
| 9 | Long term 15N studies in a catena of the shortgrass steppe显示文摘 | Delgado J A Mosier A R Valentine D W Schimel D S Parton W J | 1996 | Biogeochemistry1996，32，： | 1 |
| 10 | The response of straight mobile bed hannels to inbank and overbank flows 显示文摘 | Knight D W Brown F A Valentine E M | 1999 | Proc Instn of Civil Engineers Water Maritime and Energy Division London1999，136，4： | 1 |
| 11 | Duplex snnographie detection of internal jugular venous thrombosis after removal of central venous catheters 显示文摘 | KARNIK R VALENTIN A WINKLER WB | 1993 | Clin Cardiol1993，16，9： | 1 |
| 12 | Primary prevention of cardiovascular disease with atorvastatin in type 2 diabetes in the Collaborative Atorvastatin Diabetes Study (CARDS): multicentre randomised placebo-controlled trial显示文摘 | Helen M Colhoun D John Betteridge Paul N Durrington Graham A Hitman H Andrew W Neil Shona J Livingstone Margaret J Thomason Michael I Mackness Valentine Charlton-Menys John H Fuller | 2004 | The Lancet . 2004 (9435)2004，，9435： | 1 |
| 13 | Mechanisms involved in the conduction of anterior temporal epileptiform discharges to the scalp显示文摘 | Sparkes M Valentin A Alarcon G | 2009 | Clin Neurophysiol2009，120，12： | 1 |
| 14 | Dig- ital techniques for real - time pu/se shaping in radia- tion measurements 显示文摘 | Valentin T Jordanov G Knoll A HUBER | 1994 | Nuclear Instruments & Meth- ods in Physics Research Section A1994，353，13： | 1 |
| 15 | Patient sety in intensive care: results from the multinational Sentinel Events Evaluation (SEE) study显示文摘 | Valentin A Capuzzo M Guidet B | 2006 | Intensive care medicine2006，32，10： | 1 |
| 16 | AMPK activation: a therapeutic target for type 2 diabetes?显示文摘 | Coughlan K A Valentine R J Ruderman N B | 2014 | Diabetes Metab Syndr Obes2014，24，7： | 1 |
| 17 | Free sterol compositions from the sea cucumbers Pseudostichopus tra- chus , Holothuria (Microtele) nobilis , Holothuria scabra, Trochos- toma orientale and Bathyplotes natans 显示文摘 | VALENTINE A S LJUDMILA P P TATIANA N M | 1998 | Comp Biochem Phys B1998，120，2： | 1 |
| 18 | Theory of car- bon doping of titanium dioxide显示文摘 | Di Valentin C Pacehioni G Selloni A | 2005 | Chemistry of Mate- r/a/s2005，17，26： | 1 |
| 19 | The activated carbon electrode: A new, experimentally verified mathematical model for the potential distribution显示文摘 | CARD J C VALENTIN G STORCK A | 1990 | J Electrochem Soc1990，137，9： | 1 |
| 20 | Femtosecond Yb:YCOB laser pumped by narrow-stripe diode and passively mode locked using ion implanted saturable-absorber mirror 显示文摘 | VALENTINE G J KEMP A J B1RK1N D J L | 2000 | Electron Left2000，36，19： | 1 |
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