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| 1 | Dicoumarol enhances gemcitabine-induced cytotoxicity in high NQO1-expressing cholangiocarcinoma cells显示文摘AIM: To investigate whether dicoumarol, a potent inhibitor of NAD(P)H quinone oxidoreductase-1 (NQO1), potentiates gemcitabine to induce cytotoxicity in chol-angiocarcinoma cells (CCA) and the role of reactive oxygen generation in sensitizing the cells. METHODS: Four human cell lines with different NQO1 activity were used; the human CCA cell lines, KKU-100, KKU-OCA17, KKU-M214, and Chang liver cells. NQO1 activity and mRNA expression were determined. The cells were pretreated with dicoumarol at relevant concentrations before treatment with gemcitabine. Cytotoxicity was determined by staining with fluorescent dyes. Oxidant formation was examined by assay of cellular glu-tathione levels and reactive oxygen species production by using dihydrofluorescein diacetate. Measurement of mitochondrial transmembrane potential was performed by using JC-1 fluorescent probe. Western blotting analysis was performed to determine levels of survival related proteins. RESULTS: Dicoumarol markedly enhanced the cytotoxicity of gemcitabine in KKU-100 and KKU-OCA17, the high NQO1 activity and mRNA expressing cells, but not in the other cells with low NQO1 activity. Dicoumarol induced a marked decrease in cellular redox of gluta-thione in KKU-100 cells, in contrast to KKU-M214 cells. Dicoumarol at concentrations that inhibited NQO1 activity did not alter mitochondrial transmembrane potential and production of reactive oxygen species. Gemcitabine alone induced activation of NF-κB and Bcl-XL protein expression. However, gemcitabine and dicoumarol combination induced increased p53 and decreased Bcl-XL levels in KKU-100, but not in KKU-M214 cells. CONCLUSION: NQO1 may be important in sensitizing cells to anticancer drugs and inhibition of NQO1 may be a strategy for the treatment of CCA. | Benjaporn Buranrat Auemduan Prawan Upa Kukongviriyapan Sarinya Kong-petch Veerapol Kukongviriyapan | 2010 | World Journal of Gastroenterology2010，16，19： | 3 |
| 2 | A universal protein array system for quantitative detection of protein-protein, protein-DNA, protein-RNA and protein-ligand interactions显示文摘 | | 2000 | Nucleic Acids Research2000，28，2： | 1 |
| 3 | Mulberry leaf extract restores arterial pressure in streptozotocin-induced chronic diabetic rats显示文摘 | Jarinyaporn Naowaboot Patchareewan Pannangpetch Veerapol Kukongviriyapan Upa Kukongviriyapan Saowanee Nakmareong Arunporn Itharat | 2009 | Nutrition Research2009，，： | 1 |
| 4 | PAH Sorption Mechanism and Partitioning Behavior in Lampblack-impacted Soils from Former Oil-Gas Plant Sites 显示文摘 | Lei Hong Upa] Ghosh Tania Mahajan | 2003 | Environmental Science and Technology2003，37，： | 1 |
| 5 | The influence of patient age and mechanism of injury on the type of pelvic fracture:epidemiological study显示文摘 | Je(z)ek M D(z)upa V | | 0，，01： | 1 |
| 6 | Role of pH in electro-osmosis: Experimental study on NaC1-Water saturated kaolinite 显示文摘 | Karim Beddiar Teddy Fen-chong Andre upas | 2005 | Transport in Porous Media2005，6，： | 1 |
| 7 | Antihyperglycemic, Antioxidant and Antiglycation Activities of Mulberry Leaf Extract in Streptozotocin-Induced Chronic Diabetic Rats显示文摘 | Jarinyaporn Naowaboot Patchareewan Pannangpetch Veerapol Kukongviriyapan Bunkerd Kongyingyoes Upa kukongviriyapan | 2009 | Plant Foods for Human Nutrition2009，，2： | 1 |
| 8 | Inflammatory cytokines suppress NAD(P)H:quinone oxidoreductase-1 and induce oxidative stress in cholangiocarcinoma cells显示文摘 | Auemduan Prawan Benjaporn Buranrat Upa Kukongviriyapan Banchob Sripa Veerapol Kukongviriyapan | 2009 | Journal of Cancer Research and Clinical Oncology2009，，4： | 1 |
| 9 | Protective effects of rice bran hydrolysates on heart rate variability,cardiac oxidative stress,and cardiac remodeling in high fat and high fructose diet-fed rats显示文摘Objective:To examine the ameliorative effect of rice bran hydrolysates(RBH)on metabolic disorders,cardiac oxidative stress,heart rate variability(HRV),and cardiac structural changes in high fat and high fructose(HFHF)-fed rats.Methods:Male Sprague-Dawley rats were daily fed either standard chow diet with tap water or an HFHF diet with 10%fructose in drinking water over 16 weeks.RBH(500 and 1000 mg/kg/day)was orally administered to the HFHF-diet-fed rats during the last 6 weeks of the study period.At the end of the treatment,metabolic parameters,oxidative stress,HRV,and cardiac structural changes were examined.Results:RBH administration significantly ameliorated metabolic disorders by improving lipid profiles,insulin sensitivity,and hemodynamic parameters.Moreover,RBH restored HRV,as evidenced by decreasing the ratio of low-frequency to highfrequency power of HRV,a marker of autonomic imbalance.Cardiac oxidative stress was also mitigated after RBH supplementation by decreasing cardiac malondialdehyde and protein carbonyl,upregulating eNOS expression,and increasing catalase activity in the heart.Furthermore,RBH mitigated cardiac structural changes by reducing cardiac hypertrophy and myocardial fibrosis in HFHFdiet-fed rats.Conclusions:The present findings suggest that consumption of RBH may exert cardioprotective effects against autonomic imbalances,cardiac oxidative stress,and structural changes in metabolic syndrome. | Ketmanee Senaphan Upa Kukongviriyapan Pisit Suwannachot Geerasak Thiratanaboon Weerapon Sangartit Supawan Thawornchinsombut Akkasit Jongjareonrak | 2021 | Asian Pacific Journal of Tropical Biomedicine2021，11，5： | 0 |
| 10 | Anti-tumor activity of rice bran hydrolysates on migration, invasion and angiogenesis显示文摘Objective:To investigate anti-tumor effect of rice bran hydrolysates(RBH)on proliferation,migration,invasion,and angiogenesis of cholangiocarcinoma(CCA)cells,and elucidate the underlying mechanisms.Methods:RBH was prepared from Tubtim Chumprae rice(Oryza sativa L.)by hydrothermolysis followed by protease digestion.Phenolic content in RBH was analyzed by high-performance liquid chromatography.Human CCA cells,KKU-156,KKU-452,and KKU-100,were used to study the effects of RBH on proliferation,migration,invasion,and adhesion by wound healing,Transwell chamber,and fibronectin cell adhesion assays.Angiogenesis was evaluated using human umbilical vein endothelial cells.Proteins associated with cancer progression were analyzed by immunobloting assays.Results:RBH contained carbohydrates,proteins,lipids,and various phenolic compounds and flavonoids.RBH did not inhibit CCA proliferation,but strongly suppressed migration,invasion,adhesion of CCA cells,and the formation of tube-like capillary structures of human umbilical vein endothelial cells.Moreover,RBH downregulated phosphorylation of FAK,PI3K,and Akt,suppressed NF-κB nuclear translocation,decreased the expression of ICAM-1,vimentin and vascular endothelium growth factor(VEGF),and increased the expression of E-cadherin.Conclusions:RBH suppresses CCA cell migration and invasion and decreases expression of proteins involved in cancer metastasis.RBH is a potential food supplement for cancer prevention. | Suphanthip Phusrisom Laddawan Senggunprai Auemduan Prawan Sarinya Kongpetch Upa Kukongviriyapan Supawan Thawornchinsombut Sirithon Siriamornpun Theeraphan Chumroenphat Ronnachai Changsri Veerapol Kukongviriyapan | 2021 | Asian Pacific Journal of Tropical Biomedicine2021，11，7： | 0 |
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