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| 1 | 栓菌漆酶的异源表达及重组酶碳端和氮端组氨酸标签修饰对酶学特性的影响显示文摘以来源Trametes sp.C30的LAC3漆酶基因为研究对象,通过引物设计进行突变,在其C端和N端进行延长,分别引入6个组氨酸标签,并异源表达于酿酒酵母Saccharomyces cerevisiae中,将获得的重组酶进行比较发现漆酶末端氨基酸序列的改变对酶学性质的影响较大。C端引入组氨酸标签的重组酶的表达量不受影响,但是在N末端引入组氨酸标签的重组酶表达量只有LAC3的一半。添加组氨酸标签的融合蛋白对ABTS和SGZ两种底物的亲和力得到增强。而在酸碱稳定性耐受方面,与LAC3相比,N末端氨基酸的改变使其在酸碱稳定性方面得到了增强,中碱性条件下仍能保持较佳活性。C端和N端可塑性的研究对于漆酶新性状的获得具有重要意义。 | 刘英丽 刘骏明 王静 孙宝国 THIERRY Tron | 2014 | 食品科学2014，35，21： | 2 |
| 2 | Ischemic preconditioning protects against coronary endothelial dysfunction induced by ischemia and reperfusion显示文摘 | Kaeffer N Tron C | 1994 | Circulation1994，89，： | 1 |
| 3 | Transcatheter aortic valve implantation: technical aspects,results and indications显示文摘 | Eltchaninoff H Zaiarias A Tron C Litzler P Y Baala B Godin M | 2008 | Arch Cardiovasc Dis2008，101，： | 1 |
| 4 | Effectiveness of percutaneous mechanical mitral commissurotomy using the metallic commissurotome in patients with restenosis after balloon or previous surgical commissurotomy显示文摘 | Eltchaninoff H Tron C Cribier A | 2003 | Am J Cardiol2003，91，4： | 1 |
| 5 | Early experi- ence with percutaneoustranscatheter implantation of heart valve prosthesis for the treatment of end - stageinoperable patients with calcific aortic stenosis 显示文摘 | Cribier A Eltchaninoff H Tron C | 2004 | J Am Coil Cardi- ol2004，43，： | 1 |
| 6 | Satisfaction survey in gener- al hospital personnel involved in blood transfusion: implementation of the ISO 9001:2000 standard 显示文摘 | Chord-Auger S Tron BE Moll MC | 2004 | Transfus Clin Biol2004，11，4： | 1 |
| 7 | miR-193b Regulates Mcl-1 in Melanoma显示文摘 | Jiamin Chen Xiao Zhang Cindy Lentz Marie Abi-Daoud Geneviève C. Paré Xiaolong Yang Harriet E. Feilotter Victor A. Tron | 2011 | The American Journal of Pathology2011，，5： | 1 |
| 8 | A randomized comparison of a percutaneous suture device versus manual compression for femoral artery hemostasis after PTCA显示文摘 | Tron C Koning R Eltchaninoff H | 2003 | J Interv Cardiol2003，16，3： | 1 |
| 9 | Mathematical modeling of observed natural behavior: a fuzzy logic approach 显示文摘 | TRON E MARGALIOT M | 2004 | Fuzzy Sets and Systems2004，146，3： | 1 |
| 10 | MicroRNA-193b Represses Cell Proliferation and Regulates Cyclin D1 in Melanoma显示文摘 | Jiamin Chen Harriet E. Feilotter Geneviève C. Paré Xiao Zhang Joshua G.W. Pemberton Cherif Garady Dulcie Lai Xiaolong Yang Victor A. Tron | 2010 | The American Journal of Pathology2010，，5： | 1 |
| 11 | Click chemistry reactions in medicinal chemistry: Applications of the 1, 3-dipolar cycloaddition between azides and alkynes 显示文摘 | Tron G C Pirali T Billington R A | 2008 | Medicinal Research Reviews2008，28，2： | 1 |
| 12 | Early experience with percutaneous transcatheter implantation of heart valve prosthesis for the treatment of end-stage inoperable patients with calcific aortic stenosis显示文摘 | Cribier A Eltchaninoff H Tron C | 2004 | Am Coll Cardiol2004，43，4： | 1 |
| 13 | Characteristics of drug-related problems discussed by hospital pharmacists in multidisciplinary teams显示文摘 | Hege Salvesen Blix Kirsten K. Viktil Tron Anders Moger ?smund Reikvam | 2006 | Pharmacy World & Science2006，，3： | 1 |
| 14 | Poverty, sustainability, and household livelihood strategies in Zagros, Iran显示文摘 | Arezoo Soltani Arild Angelsen Tron Eid Mohammad Saeid Noori Naieni Taghi Shamekhi | 2012 | Ecological Economics2012，，： | 1 |
| 15 | Medicinal chemistry of combretastatin A 4: Present and future directions 显示文摘 | TRON G C PIRALI T SORBA G | 2006 | J Med Chem2006，49，1： | 1 |
| 16 | Early experience with percutaneous transcatheter implantation of heart valve prosthesis for the treatment of endstage inoperable patients with calcific aortic stenosis显示文摘 | Cribier A Eltchaninoff H Tron C Bauer F Agatiello C Sebagh L | 2004 | J Am Coll Cardiol2004，43，： | 1 |
| 17 | Early experience with percutaneous transcatheter implantation of heart valve prosthesis for the treatment of endstage inoperable patients with calcific aortic stenosis 显示文摘 | Cribier A Ehchaninoff H Tron C | 2004 | J Am Coll Cardiol2004，43，4： | 1 |
| 18 | Treatment of calcific aorta stenosis with the percutaneous heart valve midterm follow-up from the initial fasibility studies the French experience显示文摘 | CRIBIER A ELTCHANINOFF H TRON C | 2006 | J Am Coll Cardiol2006，47，： | 1 |
| 19 | Experiments on oblique rifting in brittle-ductile systems 显示文摘 | Tron V Brun J P | 1991 | Tectonophysics1991，188，12： | 1 |
| 20 | LAC3, a new low redox potential laccase from Trametes sp. strain C30 obtained as a recombinant protein in yeast显示文摘 | Agnieszka Klonowska Christian Gaudin Marcel Asso André Fournel Marius Réglier Thierry Tron | 2004 | Enzyme and Microbial Technology2004，，1： | 1 |
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