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| 1 | Dual-color deep-tissue three-photon microscopy with a multiband infrared laser显示文摘Multiphoton microscopy combined with genetically encoded fluorescent indicators is a central tool in biology.Threephoton(3P)microscopy with excitation in the short-wavelength infrared(SWIR)water transparency bands at 1.3 and 1.7μm opens up new opportunities for deep-tissue imaging.However,novel strategies are needed to enable in-depth multicolor fluorescence imaging and fully develop such an imaging approach.Here,we report on a novel multiband SWIR source that simultaneously emits ultrashort pulses at 1.3 and 1.7μm that has characteristics optimized for 3P microscopy:sub-70 fs duration,1.25 MHz repetition rate,and μJ-range pulse energy.In turn,we achieve simultaneous 3P excitation of green fluorescent protein(GFP)and red fluorescent proteins(mRFP,mCherry,tdTomato)along with third-harmonic generation.We demonstrate in-depth dual-color 3P imaging in a fixed mouse brain,chick embryo spinal cord,and live adult zebrafish brain,with an improved signal-to-background ratio compared to multicolor twophoton imaging.This development opens the way towards multiparametric imaging deep within scattering tissues. | Khmaies Guesmi Lamiae Abdeladim Samuel Tozer Pierre Mahou Takuma Kumamoto Karolis Jurkus Philippe Rigaud Karine Loulier Nicolas Dray Patrick Georges Marc Hanna Jean Livet Willy Supatto Emmanuel Beaurepaire Frédéric Druon | 2018 | Light(Science & Applications)2018，7，1： | 3 |
| 2 | High Altitude Platforms for Wireless Communications显示文摘 | TOZER T C GRACE D | 2001 | Electronics & Communication Engineering Journal2001，13，3： | 1 |
| 3 | High-altitude platforms for wireless commu- nications显示文摘 | TOZER T C GRACE D | 2001 | Electronic & Communications Engineering Journal2001，13，3： | 1 |
| 4 | Environmental factors relating to the distribution of terricolous bryophytes and lichens in semi arid eastern Australia显示文摘 | Eldridge D J Tozer M E | 1997 | Bryologist1997，100，1： | 1 |
| 5 | Distribution and floristics of bryophytes associated with soil crust in arid and semi-arid New South Wales显示文摘 | Eldridge D J Tozer M E | | 0，，： | 1 |
| 6 | Apparent diffusion coeffi- cient histograms may predict low-grade glioma subtype显示文摘 | Tozer D J Jager HR Danchaivijitr N | 2007 | NMR Bi- omed2007，20，1： | 1 |
| 7 | No water,no plate tectonics: Convective heat transfer and the planetary surface of Venus and Earth显示文摘 | Mian Z U Tozer D C | 1990 | Terra Nova1990，2，： | 1 |
| 8 | High-altitude platforms for wireless communications显示文摘 | TOZER T C GRACE D | 2001 | Electronics & Communication Engineering Journal2001，13，3： | 1 |
| 9 | Improved medium access control for data traffic via satellite using the CFDAMA ocol 显示文摘 | Mitchell P D Tozer T C Grace D | 2000 | IEE Seminar on the Critical Success Factors2000，18，： | 1 |
| 10 | Quantification of sub-tle blood-brain barrier disruption in non-enhancing lesions inmultiple sclerosis: a study of disease and lesion subtypes 显示文摘 | Soon D Tozer DJ Altmann DR | 2007 | Mult Scler2007，13，： | 1 |
| 11 | Providing multimedia communications services from high altitude platforms显示文摘 | GRACE D DALY N E TOZER T C | 2001 | International Journal of Satellite Communications2001，，19： | 1 |
| 12 | Distribution and floristics of bryophytes in soil crusts in semi-arid and arid eastern Australia 显示文摘 | ELDRIDGE D J TOZER M E | 1996 | Australian Journal of Botany1996，44，2： | 1 |
| 13 | Drug glycosides in oral colon-specificdrug delivery显示文摘 | FRIEND D R TOZER T N | | Journal of Controlled Release0，19，13： | 1 |
| 14 | High-altitude Platforms for Wireless Communications 显示文摘 | Tozer T C Grace D | 2001 | Electronics & Communication Engineering2001，6，： | 1 |
| 15 | Soil hydrology is independent of microphytic crust cover: further evidence from a wooded semiarid Australian rangeland 显示文摘 | Eldridge D J Tozer M E Slangen S | 1997 | Arid Soil Research and Rehabilitation1997，11，： | 1 |
| 16 | High-altitude platforms for wireless communications显示文摘 | Tozer D Grace | 2001 | Journal of Electronics & Communication Engineering2001，，： | 1 |
| 17 | Comparison of quantitative T2 mapping and diffusion-weighted imaging in the normal and pathologic prostate 显示文摘 | Gibbs P Tozer D J Liney GP | 2001 | Magn Reson Med2001，46，6： | 1 |
| 18 | High Altitude Platforms for Wireless Communications显示文摘 | Tozer T C Grace D | 2001 | Electronics & Communication Engineering Journal2001，13，4： | 1 |
| 19 | The Effect of Pasture Allowance and Supplementation on Feed Efficiency and Profitability of Dairy Systems显示文摘 | Tozer P R Bargo F Muller L D | 2004 | Journal of Dairy Science2004，87，： | 1 |
| 20 | Imaging cadavers:cold FLAIR and noninvasive brain thermometry using CSF diffusion显示文摘 | Tofts P S Jackson J S Tozer D J | 2008 | Magnetic Res Med2008，59，1： | 1 |
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