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| 1 | Molecularly specific detection of bacterial lipoteichoic acid for diagnosis of prosthetic joint infection of the bone显示文摘Discriminating sterile inflammation from infection, especially in cases of aseptic loosening versus an actual prosthetic joint infection, is challenging and has significant treatment implications. Our goal was to evaluate a novel human monoclonal antibody(mAb) probe directed against the Gram-positive bacterial surface molecule lipoteichoic acid(LTA). Specificity and affinity were assessed in vitro. We then radiolabeled the anti-LTA mAb and evaluated its effectiveness as a diagnostic imaging tool for detecting infection via immuno PET imaging in an in vivo mouse model of prosthetic joint infection(PJI). In vitro and ex vivo binding of the anti-LTA mAb to pathogenic bacteria was measured with Octet, ELISA, and flow cytometry. The in vivo PJI mouse model was assessed using traditional imaging modalities, including positron emission tomography(PET) with [^(18)F]FDG and [^(18)F]Na F as well as X-ray computed tomography(CT), before being evaluated with the zirconium-89-labeled antibody specific for LTA([^(89)Zr]SAC55).The anti-LTA mAb exhibited specific binding in vitro to LTA-expressing bacteria. Results from imaging showed that our model could reliably simulate infection at the surgical site by bioluminescent imaging, conventional PET tracer imaging, and bone morphological changes by CT. One day following injection of both the radiolabeled anti-LTA and isotype control antibodies, the anti-LTA antibody demonstrated significantly greater(P < 0.05) uptake at S. aureus-infected prosthesis sites over either the same antibody at sterile prosthesis sites or of control non-specific antibody at infected prosthesis sites. Taken together, the radiolabeled anti-LTA mAb, [^(89)Zr]SAC55, may serve as a valuable diagnostic molecular imaging probe to help distinguish between sterile inflammation and infection in the setting of PJI. Future studies are needed to determine whether these findings will translate to human PJI. | Julie E.Pickett John M.Thompson Agnieszka Sadowska Christine Tkaczyk Bret R.Sellman Andrea Minola Davide Corti Antonio Lanzavecchia Lloyd S.Miller Daniel LJ Thorek | 2018 | Bone Research2018，6，2： | 3 |
| 2 | Delivery platform for hydrophobic drugs: prodrug approach combined with self-assembled muhilayers 显示文摘 | THIERRY B KUJAWA P TKACZYK C | 2005 | J Am Chem Soc2005，127，6： | 1 |
| 3 | Cobah and chromium ions induce nitration of proteins in human U937 macrophages in vitro显示文摘 | Petit A Mwale F Tkaczyk C | 2006 | J Biomed Mater Res A2006，79，3： | 1 |
| 4 | Identification of anti- alpha toxin monoclonal antibodies that reduce the severity of Staphylococcus aureusdermonecrosis and exhibit a correlation between affinity and potency 显示文摘 | Tkaczyk C Hua L Varkey R | 2012 | Clin Vaccine Immunol2012，19，3： | 1 |
| 5 | Fcgamma receptors on mast cells: activatory and inhibitory regulation of mediator release显示文摘 | Tkaczyk C Okayama Y Metcalfe DD | 2004 | Int Arch Allergy Imunol2004，133，： | 1 |
| 6 | Elec trowetting of ionic liquids显示文摘 | Millefiorini S Tkaczyk A H Sedev R | 2006 | Journal of the American Chemical Society2006，128，9： | 1 |
| 7 | Snapshot image mapping spectrometer(IMS)with high sampling density for hyperspectral microscopy显示文摘 | L Gao R Kester N Hagen T Tkaczyk | 2010 | Opt Express2010，18，14： | 1 |
| 8 | Activation of human mast cells through the high affinity IgG receptor 显示文摘 | Tkaczyk C Okayama Y Wcolhiser M R | 2002 | Mol Immunol2002，38，1618： | 1 |
| 9 | Intesrated signalling pathways for mast- cell activation显示文摘 | Gilfillan A M C Tkaczyk | 2009 | Nat Rev Immunol2009，6，： | 1 |
| 10 | Integrated signalling pathways for mast-cell activation 显示文摘 | Gilfillan AM Tkaczyk C | 2006 | Nat Rev Immunol2006，6，3： | 1 |
| 11 | Activation of human mast cells through the high affinity IgG receptor显示文摘 | Okayama Y Woolhiser MR | 2002 | Mol Immunol2002，38，1618： | 1 |
| 12 | Staphylococ- cus aureus alpha toxin suppresses effective innate and adaptive immune responses in a murine dermonecrosis model 显示文摘 | TKACZYK C HAMILTON MM DATTA V | 2013 | PLoSOne2013，8，75： | 1 |
| 13 | Fc gamma receptors on mast ceils: Activatory and inhibitory regulation of mediator release 显示文摘 | Tkaczyk C Okayama Y Metcalfe DD | 2004 | Int Arch Allergy Immunol2004，133，： | 1 |
| 14 | Electrowet-ting of ionic liquids显示文摘 | MILLEFIORINI S TKACZYK A H SEDEV R | 2006 | JACS2006，128，： | 1 |
| 15 | Unusual bilateral obstructive uropathy with kidney failure in an adolescent with ulcerative colitis 显示文摘 | Tkaczyk M Katu~yfaska A Samolewicz E | 2012 | J Crohns Colitis2012，6，2： | 1 |
| 16 | Comparison of Fc epsilon RI-and Fc gamma RI-mediated degranulation and TNF-alpha synthesis in human mast cells: selective utilization of phosphatidylinositol-3- kinase for Fc gamma Ri-induced degranulation 显示文摘 | Okayama Y Tkaczyk C Metcalfe DD | 2003 | European J Immunol2003，33，： | 1 |
| 17 | Continuous veno-venous hemodiafiltration in children after cardiac surgery显示文摘 | Anna Jander Marcin Tkaczyk Izabela P?gowska-Klimek Witold Pietrzykowski Jacek Moll Wojciech Krajewski Micha? Nowicki | 2007 | European Journal of Cardio-Thoracic Surgery2007，，6： | 1 |
| 18 | Integrated signalling pathwaysfor mast-cell activation显示文摘 | GILFILLAN A M TKACZYK C | 2006 | Nat Rev lmmtmol2006，6，3： | 1 |
| 19 | Heterogeneity of Strengthening of the Thick-Walled Copper Square Tubes in a Process of Rolling in a Four Roll Pass 显示文摘 | Oczkowicz T Tkaczyk S | 1997 | Journal of Materials Processing Technology1997，64，1： | 1 |
| 20 | Differential antibac- terial activity of genistein arising from global inhibition of DNA, RNA and protein synthesis in some bacterial strains 显示文摘 | Ulanowska K Tkaczyk A Konopa G | 2006 | Archives of Microbiology2006，184，5： | 1 |
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