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| 1 | Onco-miR-24 regulates cell growth and apoptosis by targeting BCL2L11 in gastric cancer显示文摘胃的癌症是世界范围的最普通的恶意之一;然而,在 tumorigenesis 的分子的机制仍然需要探索。BCL2L11 属于 BCL-2 家庭,并且充当内在的 apoptotic 串联的一个中央管理者并且调停房间 apoptosis。尽管 miRNAs 被报导了涉及癌症开发的每个阶段,在 GC 的 miR-24 的角色还没被报导。在现在的学习,当 BCL2L11 的表示在 GC 的肿瘤纸巾被禁止时, miR-24 被发现起来调整。研究从在 vitro 并且在显示出的 vivo,那 miR-24 调整 BCL2L11 表示由与 mRNA 的 3UTR 直接有约束力,因此支持房间生长,移植当禁止房间 apoptosis 时。因此, miR-24 是能是为未来的潜在的药目标的新奇 onco-miRNA 临床的使用。 | Haiyang Zhang Jingjing Duan Yanjun Qu Ting Deng Rui Liu Le Zhang Ming Bai Jialu Li Tao Ning Shaohua Ge Xia Wang ZhenzhenWang~ Qian Fan Hongli Li Guoguang Ying Dingzhi Huang Yi Ba | 2016 | Protein & Cell2016，7，2： | 11 |
| 2 | PRDM14 Promotes the Migration of Human Non-small Cell Lung Cancer Through Extracellular Matrix Degradation in vitro显示文摘 | Hong-Xia Bi Han-Bing Shi Ting Zhang Ge Cui | 2015 | Chinese Medical Journal2015，，3： | 10 |
| 3 | Establishing a human pancreatic stem cell line and transplanting induced pancreatic islets to reverse experimental diabetes in rats显示文摘The major obstacle in using pancreatic islet transplantation to cure type I and some type II diabetes is the shortage of the donors. One of ways to overcome such obstacle is to isolate and clone pancreatic stem cells as 'seed cells' and induce their differentiation into functional islets as an abundant trans-plantation source. In this study, a monoclonal human pancreatic stem cell (mhPSC) line was obtained from abortive fetal pancreatic tissues. Pancreatic tissues were taken from abortive fetus by sterile procedures, and digested into single cells and cell clusters with 0.1% type IV collagenase. Cultured in modified glucose-low DMEM with 10% fetal bovine serum (FBS), these single cells and cell clusters adhered to culture dishes, and then primary epidermal-like pancreatic stem cells started to clone. After digesting with 0.25% trypsin and 0.04% EDTA, fibroblasts and other cells were gradually eliminated and epithelioid pancreatic stem cells were gradually purified during generations. Using clone-ring selection, the mhPSCs were obtained. After addition of 10 ng/mL epidermal growth factor (EGF) in cell culture medium, the mhPSCs quickly grew and formed a gravelstone-like monolayer. Continuously proliferated, a mhPSC line, which was derived from a male abortive fetus of 4 months old, has been passed through 50 generations. More than 1×109 mhPSCs were cryo-preserved in liquid nitrogen. Karyotype analysis showed that the chromosome set of the mhPSC line was normal diploid. Immunocytochemistry results demonstrated that the mhPSC line was positive for the pdx1, glucagon, nestin and CK19, and negative for the insulin, CD34, CD44 and CD45 protein expression. RT-PCR revealed further that the mhPSCs expressed transcription factors of the pdx1, glucagon, nestin and CK19. Also, in vitro induced with β-mercaptoethanol, the mhPSCs differentiated into nerve cells that expressed the NF protein. Induced with nicotinamide, the mhPSCs differentiated into functional islet-like clusters, as identified by di-thizone staining, which expressed the transcription factor of the insulin and secreted the insulin and C-peptide. Furthermore, the transplantation of mhPSCs-induced pancreatic islets into the subcapsular region of the kidney in streptozotocin-induced diabetic rats could reduce blood glucose levels and prolong the life time. | XIAO Mei AN LiLong YANG XueYi GE Xin QIAO Hai ZHAO Ting MA XiaoFei FAN JingZhuang ZHU MengYang DOU ZhongYing | 2008 | Science China(Life Sciences)2008，51，9： | 9 |
| 4 | Scorpion Venom Heat-Resistant Peptide is Neuroprotective against Cerebral Ischemia-Reperfusion Injury in Association with the NMDA-MAPK Pathway显示文摘Scorpion venom heat-resistant peptide(SVHRP)is a component purified from Buthus martensii Karsch scorpion venom. Our previous studies have shown that SVHRP is neuroprotective in models of Alzheimer’s disease and Parkinson’s disease. The present study aimed to explore the potential neuroprotective effects of SVHRP on cerebral ischemia/reperfusion(I/R) injury, using a mouse model of middle cerebral artery occlusion/reperfusion(MCAO/R) and a cellular model of oxygen-glucose deprivation/reoxygenation(OGD/R). Our results showed that SVHRP treatment decreased the neurological deficit scores, edema formation, infarct volume and neuronal loss in the MCAO/R mice, and protected primary neurons against OGD/R insult. SVHRP pretreatment suppressed the alterations in protein levels of N-methyl-D-aspartate receptors(NMDARs) and phosphorylated p38 MAPK as well as some proinflammatory factors in both the animal and cellular models. These results suggest that SVHRP has neuroprotective effects against cerebral I/R injury, which might be associated with inhibition of the NMDA-MAPKmediated excitotoxicity. | Xu-Gang Wang Dan-Dan Zhu Na Li Yue-Lin Huang Ying-Zi Wang Ting Zhang Chen-Mei Wang Bin Wang Yan Peng Bi-Ying Ge Shao Li Jie Zhao | 2020 | Neuroscience Bulletin2020，36，3： | 8 |
| 5 | An increase in corn resistant starch decreases protein fermentation and modulates gut microbiota during in vitro cultivation of pig large intestinal inocula显示文摘High-protein diet could cause an increase in protein fermentation in the large intestine, leading to an increased production of potentially detrimental metabolites. We hypothesized that an increase in corn resistant starch content may attenuate the protein fermentation. The aim of this study was to evaluate the effect of resistant starch on protein fermentation by inocula from large intestine of pigs using in vitro cultivation. Fermentation patterns were analyzed during a 24-h incubation of cecal and colonic digesta with varying corn resistant starch contents, using casein protein as sole nitrogen source. The results showed that the concentration of short-chain fatty acids(SCFA) and cumulative gas production were significantly increased(P < 0.05), while ammonia-nitrogen(NH_3-N) and branched-chain fatty acids(BCFA), which indicated protein fermentation, decreased when the corn resistant starch levels increased(P < 0.05). The copies of total bacteria, Bifidobacterium and Lactobacillus were significantly increased with the increased corn resistant starch levels after incubation(P < 0.05). The copies of the Bifidobacterium and Lactobacillus in cecum were significantly higher than those in colon(P < 0.05). We conclude that the addition of corn resistant starch weakens the protein fermentation by influencing microbial population and reducing protein fermentation in the cecum and colon in vitro. | Xiangyu He Weiwei Sun Ting Ge Chunlong Mu Weiyun Zhu | 2017 | Animal Nutrition2017，，3： | 8 |
| 6 | Chromosome-level genome of Himalayan yew provides insights into the origin and evolution of the paclitaxel biosynthetic pathway显示文摘Taxus,commonly known as yew,is a well-known gymnosperm with great ornamental and medicinal value.In this study,by assembling a chromosome-level genome of the Himalayan yew(Taxus wallichiana)with 10.9 Gb in 12 chromosomes,we revealed that tandem duplication acts as the driving force of gene family evolution in the yew genome,resulting in the main genes for paclitaxel biosynthesis,i.e.those encoding the taxadiene synthase,P450s,and transferases,being clustered on the same chromosome.The tandem duplication may also provide genetic resources for the nature to sculpt the core structure of taxoids at different positions and subsequently establish the complex pathway of paclitaxel by neofunctionalization.Furthermore,we confirmed that there are two genes in the cluster encoding isoenzymes of a known enzyme in the paclitaxel biosynthetic pathway.The reference genome of the Himalayan yew will serve as a platform for decoding the complete biosynthetic pathway of paclitaxel and understanding the chemodi-versity of taxoids in gymnosperms. | Jian Cheng Xiao Wang Xiaonan Liu Xiaoxi Zhu Zihe Li Huanyu Chu Qian Wang QianQian Lou Bijun Cai Yiqun Yang Xiaoyun Lu Kai Peng Dingyu Liu Yuwan Liu Lina Lu Huan Liu Ting Yang Qijin Ge Chengcheng Shi Guichun Liu Zhiwei Dong Xun Xu Wen Wang Huifeng Jiang Yanhe Ma | 2021 | Molecular Plant2021，14，7： | 6 |
| 7 | Herpes simplex virus-1 infection or Simian virus 40-mediated immortalization of corneal cells causes permanent translocation of NLRP3 to the nuclei显示文摘AIM: To investigate into the potential involvement of pyrin containing 3 gene(NLRP3), a member of the nucleotide-binding oligomerization domain-like receptors with cytosolic pattern recognition, in the host defense of corneas against viruses.METHODS: The herpes viral keratitis model was utilized in BALB/c mice with inoculation of herpes simplex virus-1(HSV-1). Corneal tissues removed during therapy of patients with viral keratitis as well as a Simian vacuolating virus 40(SV40)-immortalized human corneal epithelial cell line were also examined.Immunohistochemistry was used to detect NLRP3 in these subjects, focusing on their distribution in tissue or cells. Western blot was used to measure the level of NLRP3 and another two related molecules in NLPR3 inflammasome, namely caspase-1 and IL-1β.RESULTS: The NLRP3 activation induced by HSV-1infection in corneas was accompanied with redistribution of NLRP3 from the cytoplasm to the nucleus in both murine and human corneal epithelial cells. Furthermore,in the SV40-immortalized human corneal epithelial cells,NLRP3 was exclusively located in the nucleus, and treatment of the cells with high concentration of extracellular potassium(known as an inhibitor of NLRP3activation) effectively drove NLRP3 back to the cytoplasm as reflected by both immunohistochemistry and Western blot.· CONCLUSION: It is proposed that herpes virus infection activates and causes redistribution of NLRP3 to nuclei. Whether this NLRP3 translocation occurs with other viral infections and in other cell types merit further study. | Shu-Long Wang Ge Zhao Wei Zhu Xiao-Meng Dong Ting Liu Yuan-Yuan Li Wen-Gang Song Yi-Qiang Wang | 2015 | International Journal of Ophthalmology(English edition)2015，8，1： | 5 |
| 8 | High He-ion irradiation resistance of CrMnFeCoNi high-entropy alloy revealed by comparison study with Ni and 304SS显示文摘High entropy alloys(HEAs) have presented potential applications in nuclear power plants owing to their novel atomic structure based high irradiation resistance. However, understanding of He-ion irradiation of HEAs is still lacking. In this work, we reveal He-ion irradiation resistance of HEA CrMnFeCoNi by comparison study with a pure Ni and a 304 stainless steel(304SS). It is found that the damage structure in the three materials can be characterized with He bubbles and stacking faults/stacking fault tetrahedrons((SFs/SFTs), which show a similar depth distribution after He-ion irradiation at both RT and 450℃.Although the He bubbles have a similar size about 2nm after irradiation at RT, the He bubble sizes of the HEA, 304SS, and Ni increase to 4.0±0.9,5.3±1.0 and 6.7 ±1.0 nm after irradiation at 450℃, respectively. Moreover, the density of SFs/SFTs displays in an order of Ni < 304 SS < HEA at both RT and 450℃.The He-ion irradiation at RT causes significant hardness enhancement for the three materials, however,compared to RT, after irradiation at 450℃, the Ni presents softening, while the 304SS, especially the HEA,shows further hardening. Thus, the HEA CrMnFeCoNi possesses the smallest He bubble size, the densest SFs/SFTs, and the highest hardening, indicating the best structural stability, as well as the best He-ion irradiation resistance, which can be attributed to its low mobility of He atoms and point defects. | Lixin Yang Hualong Ge Jian Zhang Ting Xiong Qianqian Jin Yangtao Zhou Xiaohong Shao Bo Zhang Zhengwang Zhu Shijian Zheng Xiuliang Ma | 2019 | Journal of Materials Science & Technology2019，35，3： | 5 |
| 9 | MIMO SAR Waveform Separation Based on Costas-LFM Signal and Co-arrays for Maritime Surveillance显示文摘In case of bright targets over a dark background, the cross-correlation noise of Multiple input and multiple output(MIMO) Synthetic aperture radar(SAR) orthogonal waveforms in the same frequency coverage will increase which can degrade the image. An Interpulse Costas hopping and intra-pulse Slope coded linearly frequency modulated(IPC-SCLFM) waveform is designed and analyzed to decrease the autocorrelation and cross-correlation noise. Besides a novel acquisition mode of 'MIMO Coprime SAR'(MIMO-Cop SAR) is proposed with Coprime arrays(Co-arrays) in receive across the SAR line of flight based on digital beamforming. An improved processing by multiplying values at each pixel of two coarrays images is proposed to suppress the cross-correlation noise. MIMO-Cop SAR can effectively suppress the crosscorrelation noise, reduce the amount of data to be stored and get high signal-to-noise ratio in a simple way. The effectiveness of MIMO-Cop SAR for maritime surveillance is demonstrated by the simulation experiments. | LIU Feng MU Shanxiang LYU Wanghan LI Wenliang GE Ting | 2017 | Chinese Journal of Electronics2017，26，1： | 5 |
| 10 | Treatment results and prognostic factors of patients undergoing postoperative radiotherapy for laryngeal squamous cell carcinoma显示文摘Postoperative radiotherapy (PRT) is widely advocated for patients with squamous cell carcinomas of the head and neck that are considered to be at high risk of recurrence after surgical resection. The aims of this study were to evaluate the treatment outcomes of PRT for patients with laryngeal carcinoma and to identify the value of several prognostic factors. We reviewed the records of 256 patients treated for laryngeal squamous cell carcinoma between January 1993 and December 2005. Disease-free survival (DFS) and overall survival (OS) were estimated using the Kaplan-Meier method. Log-rank test was employed to identify significant prognostic factors for DFS and OS. The Cox proportional hazards model was applied to identify covariates significantly associated with the aforementioned endpoints. Our results showed the 3-, 5-, and 10-year DFS for all patients were 69.9%, 59.5%, and 34.9%, respectively. The 3-, 5-, and 10-year OS rates were 80.8%, 68.6%, and 38.8%, respectively. Significant prognostic factors for both DFS and OS on univariate analysis were grade, primary site, T stage, N stage, overall stage, lymph node metastasis, overall treatment times of radiation, the interval between surgery and radiotherapy, and radiotherapy equipment. Favorable prognostic factors for both DFS and OS on multivariate analysis were lower overall stage, no cervical lymph node metastasis, and using 60Co as radiotherapy equipment. In conclusion, our data suggest that lower overall stage, no cervical lymph node metastasis, and using 60Co as radiotherapy equipment are favorable prognostic factors for DFS and OS and that reducing the overall treatment times of radiation to 6 weeks or less and the interval between surgery and radiotherapy to less than 3 weeks are simple measures to remarkably improve treatment outcome. | Ting Jin Wei-Han Hu Li-Bing Guo Wen-Kuan Chen Qiu-Li Li Hui Lin Xiu-Yu Cai Nan Ge Rui Sun Si-Yi Bu Xin Zhang Meng-Yao Qiu Wei Zhang Su Luo Yi-Xin Zhou | 2011 | Chinese Journal of Cancer2011，30，7： | 5 |
| 11 | Pluripotent Stem Cells Induced from Mouse Somatic Cells by Small-Molecule Compounds显示文摘 | Pingping Hou Yanqin Li Xu Zhang Chun Liu Jingyang Guan Honggang Li Ting Zhao Junqing Ye Weifeng Yang Kang Liu Jian Ge Jun Xu Qiang Zhang Yang Zhao Hongkui Deng | 2013 | Science2013，，6146： | 4 |
| 12 | Clinical correlation of B7-H3 and B3GALT4 with the prognosis of colorectal cancer显示文摘AIM To investigate the expression and clinical significance of B7 homolog 3(B7-H3) and β-1,3-galactosyltransferase-4(B3 GALT4) in colorectal cancer(CRC) patients.METHODS Using tissue microarray, we identified the expression of B7-H3 and B3 GALT4 in 223 CRC patient samples by immunohistochemistry and evaluated the possible correlation between B7-H3 and B3 GALT4 and clinicaloutcomes. Further, the m RNA and protein expression were identified to establish the regulatory relationship of B7-H3 with B3 GALT4 in vitro.RESULTS A significant positive correlation between B7-H3 and B3 GALT4 was observed in CRC specimens(r = 0.219, P = 0.001). High expression of B7-H3 was identified as a significant independent predictor of poor overall survival(OS) [hazard ratio(HR) = 1.781; 95%CI: 1.027-3.089; P = 0.040]. Moreover, high expression of B3 GALT4 was also recognized as an independent predictor of inferior OS(HR = 1.597; 95%CI: 1.007-2.533; P = 0.047). Additionally, CRC patients expressing both high B7-H3 and high B3 GALT4 contributed to a significant decrease in OS(HR = 2.283; 95%CI: 1.289-4.042; P = 0.005). In CRC cell lines with stable expression of high B7-H3, the m RNA and protein expressions of B3 GALT4 were significantly upregulated. Similarly, the expression of B3 GALT4 was significantly reduced when expression of B7-H3 was knocked down.CONCLUSION The expression of B3 GALT4 in CRC is positively correlated with B7-H3 expression in vitro. B7-H3/B3 GLAT4 may be used as dual prognostic biomarkers for CRC. | Ting Zhang Fang Wang Jing-Yi Wu Zhi-Chao Qiu Yan Wang Fen Liu Xiao-Song Ge Xiao-Wei Qi Yong Mao Dong Hua | 2018 | World Journal of Gastroenterology2018，24，31： | 4 |
| 13 | Arabidopsis ROOT HAIR DEFECTIVE3 is involved in nitrogen starvation-induced anthocyanin accumulation显示文摘Anthocyanin accumulation is a common phenomenon seen in plants under environmental stress. In this study,we identified a new allele of ROOT HAIR DEFECTIVE3(RHD3)showing an anthocyanin overaccumulation phenotype under nitrogen starvation conditions. It is known that ethylene negatively regulates light- and sucrose-induced anthocyanin biosynthesis. We hypothesized that RHD3 achieves its negative effect on anthocyanin biosynthesis via an ethylene-regulating pathway. In support of this, similar to rhd3 mutants, the Arabidopsis ethylene signaling mutants etr1, ein2, and ein3/eil1 showed an anthocyanin overaccumulation phenotype under nitrogen starvation conditions. The ethylene precursor ACC strongly suppressed anthocyanin accumulation, dependent on ETR1, EIN2, EIN3/EIL1, and, partially, RHD3. In addition,inactivating RHD3 partially reversed the suppressive effect of ETO1 inactivation-evoked endogenous ethylene production on anthocyanin accumulation. The expression of nitrogen starvation-induced anthocyanin biosynthesis genes was negatively regulated by RHD3, but ethylene response genes were Researchpositively regulated by RHD3. Wild-type seedlings overexpressing RHD3 showed similar phenotypes to rhd3 mutants,indicating the existence of a fine-tuned relationship between gene expression and function. RHD3 was initially identified as a gene involved in root hair development. This study uncovered a new physiological function of RHD3 in nitrogen starvationinduced anthocyanin accumulation and ethylene homeostasis. | Jing Wang Yan Wang Ju Yang Chunli Ma Ying Zhang Ting Ge Zhi Qi Yan Kang | 2015 | Journal of Integrative Plant Biology2015，57，8： | 3 |
| 14 | Modulation of the pentose phosphate pathway alters phase I metabolism of testosterone and dextromethorphan in HepG2 cells显示文摘目的: 戊糖磷酸盐小径(PPP ) 涉及 glucose-6-phosphate 脱氢酶(G6PD ) 和 NADPH 的产生的活动,它在药新陈代谢起一个关键作用。这研究的目的是在 vitro 在药新陈代谢能力上调查 PPP 的调整的效果。 | Wen-jing XIAO Ting MA Chun GE Wen-juan XIA Yong MAO Run-bin SUN Xiao-yi YU Ji-ye AA Guang-ji WANG | 2015 | Acta Pharmacologica Sinica2015，36，2： | 3 |
| 15 | Anti-cancer Effect of 20(S)-Ginsenoside-Rh2 on Oral Squamous Cell Carcinoma Cells via the Decrease in ROS and Downregulation of MMP-2 and VEGF显示文摘Squamous cell carcinoma(SCC) is the most common in oral and maxillofacial regions and occupy more than 90% of the malignant lesions in the oral cavity[1]. The complex anatomy of the head and neck region gives rise to intricate patterns of local invasion and regional spread, which makes primary tumors more difficult to eradicate. | ZHANG Bao Ping LI Bo CHENG Jing Yang CAO Rui GAO Shu Ting HUANG Chun Juan LI Rui Ping NING Jing LIU Bin LI Zhi Ge | 2020 | Biomedical and Environmental Sciences2020，33，9： | 3 |
| 16 | Pharmacokinetic interactions between 20(S)- ginsenoside Rh2 and the HIV protease inhibitor ritonavir in vitro and in vivo显示文摘 | Jian SHI Bei CAO Wei-bin ZHA Xiao-lan WU Lin-sheng LIU Wen-jing XIAO Rong-rong GU Run-bin SUN Xiao-y YU Tian ZHENG Meng-jie LI Xin-wen WANG Jun ZHOU Yong MAO Chun GE Ting MA Wen-juan XIA Ji-ye AA Guang-ji WANG Chang-xiao LIU | 2013 | Acta Pharmacologica Sinica2013，34，10： | 3 |
| 17 | Micro RNA-548a-5p promotes proliferation and inhibits apoptosis in hepatocellular carcinoma cells by targeting Tg737显示文摘AIM: To investigate whether Tg737 is regulated by micro RNA-548a-5p(mi R-548a-5p), and correlates with hepatocellular carcinoma(HCC) cell proliferation and apoptosis.METHODS: Assays of loss of function of Tg737 were performed by the colony formation assay, CCK assay and cell cycle assay in HCC cell lines. The interaction between mi R-548a-5p and its downstream target, Tg737, was evaluated by a dual-luciferase reporter assay and quantitative real-time polymerase chain reaction. Tg737 was then up-regulated in HCC cells to evaluate its effect on mi R-548a-5p regulation. Hep G2 cells stably overexpressing mi R-548a-5p or mi R-control were also subcutaneously inoculated into nude mice to evaluate the effect of mi R-548a-5p up-regulation on in vivo tumor growth. As the final step, the effect of mi R-548a-5p on the apoptosis induced by cisplatin was evaluated by flow cytometry.RESULTS: Down-regulation of Tg737, which is a target gene of mi R-548a-5p, accelerated HCC cell proliferation, and mi R-548a-5p promoted HCC cell proliferation in vitro and in vivo. Like the downregulation of Tg737, overexpression of mi R-548a-5p in HCC cell lines promoted cell proliferation, increased colony forming ability and hampered cell apoptosis. In addition, mi R-548a-5p overexpression increased HCC cell growth in vivo. Mi R-548a-5p downregulated Tg737 expression through direct contact with its 3' untranslated region(UTR), and mi R-548a-5p expression was negatively correlated with Tg737 levels in HCC specimens. Restoring Tg737(without the 3'UTR) significantly hampered mi R-548a-5p induced cell proliferation, and rescued the mi R-548a-5p induced cell proliferation inhibition and apoptosis induced by cisplatin.CONCLUSION: Mi R-548a-5p negatively regulates the tumor inhibitor gene Tg737 and promotes tumorigenesis in vitro and in vivo, indicating its potential as a novel therapeutic target for HCC. | Ge Zhao Ting Wang Qi-Ke Huang Meng Pu Wei Sun Zhuo-Chao Zhang Rui Ling Kai-Shan Tao | 2016 | World Journal of Gastroenterology2016，22，23： | 3 |
| 18 | Toll-like receptor 5-mediated signaling enhances liver regeneration in mice显示文摘Background:Toll-like receptor 5(TLR5)-mediated pathways play critical roles in regulating the hepatic immune response and show hepatoprotective effects in mouse models of hepatic diseases.However,the role of TLR5 in experimental models of liver regeneration has not been reported.This study aimed to investigate the role of TLR5 in partial hepatectomy(PHx)-induced liver regeneration.Methods:We performed 2/3 PHx in wild-type(WT)mice,TLR5 knockout mice,or TLR5 agonist CBLB502 treated mice,as a model of liver regeneration.Bacterial flagellin content was measured with ELISA,and hepatic TLR5 expression was determined with quantitative PCR analyses and flow cytometry.To study the effects of TLR5 on hepatocyte proliferation,we analyzed bromodeoxyuridine(BrdU)incorporation and proliferating cell nuclear antigen(PCNA)expression with immunohistochemistry(IHC)staining.The effects of TLR5 during the priming phase of liver regeneration were examined with quantitative PCR analyses of immediate early gene mRNA levels,and with Western blotting analysis of hepatic NF-κB and STAT3 activation.Cytokine and growth factor production after PHx were detected with real-time PCR and cytometric bead array(CBA)assays.Oil Red O staining and hepatic lipid concentrations were analyzed to examine the effect of TLR5 on hepatic lipid accumulation after PHx.Results:The bacterial flagellin content in the serum and liver increased,and the hepatic TLR5 expression was significantly up-regulated in WT mice after PHx.TLR5-deficient mice exhibited diminished numbers of BrdU-and PCNA-positive cells,suppressed immediate early gene expression,and decreased cytokine and growth factor production.Moreover,PHx-induced hepatic NF-κB and STAT3 activation was inhibited in Tlr5–/–mice,as compared with WT mice.Consistently,the administration of CBLB502 significantly promoted PHx-mediated hepatocyte proliferation,which was correlated with enhanced production of proinflammatory cytokines and the recruitment of macrophages and neutrophils in the liver.Furthermore,Tlr5–/–mice displayed significantly lower hepatic lipid concentrations and smaller Oil Red O positive areas than those in control mice after PHx.Conclusions:We reveal that TLR5 activation contributes to the initial events of liver regeneration after PHx.Our findings demonstrate that TLR5 signaling positively regulates liver regeneration and suggest the potential of TLR5 agonist to promote liver regeneration. | Wen Zhang Lei Wang Xue-Hua Sun Xian Liu Yang Xiao Jie Zhang Ting Wang Hui Chen Yi-Qun Zhan Miao Yu Chang-Hui Ge Chang-Yan Li Guang-Ming Ren Rong-Hua Yin Xiao-Ming Yang | 2021 | Military Medical Research2021，8，4： | 3 |
| 19 | Hydraulic calculation and dynamic analysis of columnar reversing gate显示文摘According to the hydraulic calculation principles of the orifice outflow, the discharge capacity of the columnar reversing gate under the partial opening condition was calculated and checked. Using ANSYS, a large finite element analysis software, the discharge process was simulated. The distribution rule of the velocities in the gate chamber and downstream channel was obtained. An FEM model of the columnar reversing gate was built, and the natural vibration properties of the gate were analyzed. Based on the Westergaard added mass method, the added mass caused by the fluid-structure coupling motion was taken into account, and the effects of the coupling interaction were discussed. The results show that the size of the small gates meets the demand for discharge capacity, the current in the gate chamber is quite turbulent, the trunnion and arms are obviously impacted by flow, and the effects of water on vibration characteristics are remarkable. The study provides a reference for the design and calculation of gates of the same type. | You-an HU Meng WANG Miao-miao WANG Ge SONG Wen-xuan NIU Ting XU | 2011 | Water Science and Engineering2011，4，3： | 3 |
| 20 | Inhibition of Nonsmall Cell Lung Cancer Cell Migration by Protein Arginine Methyltransferase 1-small Hairpin RNA Through Inhibiting Epithelial-mesenchymal Transition,Extracellular Matrix Degradation, and Src Phosphorylation In Vitro显示文摘 | Ting Zhang Ge Cui Yun-Liang Yao Yue Guo Qi-Chun Wang Xi-Ning Li Wen-Ming Feng | 2015 | Chinese Medical Journal2015，，9： | 3 |
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