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| 1 | Development of species-specific SCAR markers for identification of three medicinal species of Phyllanthus显示文摘Phyllanthus amarus Schum. & Thonn. has been widely used in traditional medicine in Thailand as an antipyretic, a diuretic, to treat liver diseases and viral infections. Two closely related species, P. debilis L. and P. urinaria Klein ex Willd., with different and less effective medicinal properties, are less commonly used. These three species are similar in morphology and often occur in overlapping populations in nature. The latter two species can easily be mistaken for P. amarus and collected for medicinal uses, which can lead to undesirable results. DNA fingerprints of these species were obtained using RAPD-PCR techniques. RAPD markers specific for each species were identified. Primers for highly specific sequence-characterized-amplified-regions (SCAR) were then designed from nucleotide sequences of specific RAPD markers. These primers efficiently amplified SCAR markers of 408, 501 and 319 bp unique to P. amarus, P. debilis and P. urinaria respectively. This method of plant identification was rapid and highly specific when tested against DNA of several closely related species and was able to amplify specific markers from mixed DNA samples. | Piyada THEERAKULPISUT Nantawan KANAWAPEE Duangkamol MAENSIRI Sumontip BUNNAG Pranom CHANTARANOTHAI | 2008 | 植物分类学报2008，46，4： | 4 |
| 2 | Differential Proteins Expressed in Rice Leaves and Grains in Response to Salinity and Exogenous Spermidine Treatments显示文摘Exogenous application of spermidine(Spd) has been reported to modulate physiological processes and alleviate salt-induced damage to growth and productivity of several plants including rice. Employing a proteomic approach, we aimed at identifying rice leaf and grain proteins differentially expressing under salt stress, and in response to Spd prior to Na Cl treatment. A total of 9 and 20 differentially expressed protein spots were identified in the leaves of salt-tolerant(Pokkali) and saltsensitive(KDML105) rice cultivars, respectively. Differential proteins common to both cultivars included a photosynthetic light reaction protein(oxygen-evolving complex protein 1), enzymes of Calvin cycle and glycolysis(fructose-bisphosphate aldolase and triose-phosphate isomerase), malate dehydrogenase, superoxide dismutase and a hypothetical protein(Os I_18213). Most proteins were present at higher intensities in Pokkali leaves. The photosynthetic oxygen-evolving enhancer protein 2 was detected only in Pokkali and was up-regulated by salt-stress and further enhanced by Spd treatment. All three spots identified as superoxide dismutase in KDML105 were up-regulated by Na Cl but down-regulated when treated with Spd prior to Na Cl, indicating that Spd acted directly as antioxidants. Important differential stress proteins detected in mature grains of both rice cultivars were late embryogenesis abundant proteins with protective roles and an antioxidant protein, 1-Cys-peroxiredoxin. Higher salt tolerance of Pokkali partly resulted from higher intensities and more responsiveness of the proteins relating to photosynthesis light reactions, energy metabolism, antioxidant enzymes in the leaves, and stress proteins with protective roles in the grains. | Paweena SALEETHONG Sittiruk ROYTRAKUL Kanlaya KONG-NGERN Piyada THEERAKULPISUT | 2016 | Rice science2016，23，1： | 2 |
| 3 | Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass poUen显示文摘 | Singh MB Hough T Theerakulpisut P | 1991 | Proc Natl Acad Sci USA1991，88，4： | 1 |
| 4 | Drying characteristics of Shiitake mushroom and Jinda chili during vacuum heat pump drying显示文摘 | ARTNASEAW A THEERAKULPISUT S BENJAPIYAPORN C | 2010 | Food and Bioproducts Processing2010，88，23： | 1 |
| 5 | Thin Layer Drying of Carrot Slices by Heat Pump Vacuum Dryer 显示文摘 | Luampon R Theerakulpisut S Artnaseaw A | 2013 | Advanced Science Letters2013，19，11： | 1 |
| 6 | Modeling of a split type air conditioner with integrated water heater 显示文摘 | Techarungpaisan P Theerakulpisut S Priprem S | 2007 | Energy Conversion and Management2007，48，4： | 1 |
| 7 | Exogenous proline and trehalose promote recovery of rice seedlings from salt-stress and differentially modulate antioxidant enzymes and expression of related genes显示文摘 | Nounjan N Nghia PT Theerakulpisut P | | 0，，06： | 1 |
| 8 | Modeling of a split type air conditioner with integrated water heater显示文摘 | TECHARUNGPAISAN P THEERAKULPISUT S PRIPREM S | 2007 | Energy Conversion and Management2007，，48： | 1 |
| 9 | Drying characteristics of Shiitake mushroom and Jinda chili during vacuum heat pump drying显示文摘 | Artnaaeaw Theerakulpisut S Benjapiyaporn C | | 0，，2： | 1 |
| 10 | Exogenous proline and trehalose promote recovery of rice seedlings from salt-stress and differentially modulate antioxidant enzymes and expression of related genes显示文摘 | Noppawan Nounjan Phan Tuan Nghia Piyada Theerakulpisut | 2012 | Journal of Plant Physiology2012，，6： | 1 |
| 11 | Drying characteristics of Shiitake mushroom and Jin- da chili during vacuum heat pump dries显示文摘 | Apichart Artnaseaw Somnuk Theerakulpisut Chatchai Ben- japiyaporn | 2010 | Food and Bioproducts Processing2010，，88： | 1 |
| 12 | Modeling cooling coils 显示文摘 | Theerakulpisut S Prinprem S | 1998 | International Communications in Heat and Mass Transfer1998，25，1： | 1 |
| 13 | Isolation of a gene preferentially expressed in mature anthers of rice (Oryza sativa L.)显示文摘 | H. Xu P. Theerakulpisut P. E. Taylor R. B. Knox M. B. Singh P. L. Bhalla | 1995 | Protoplasma (-)1995，，1： | 1 |
| 14 | Exogenous proline and trehalose promote recovery of rice seedlings from salt-stress and differentially modulate antioxidant enzymes and expression of related genes显示文摘 | Nounjan N Nghia P T Theerakulpisut P | 2012 | J Plant Physiol2012，169，6： | 1 |
| 15 | Drying characteristics of Shiitake mushroom and Jinda chili during vacuum heat pump drying显示文摘 | Artnaseaw A Theerakulpisut S Benjapiyaporn C | 2010 | Food and Bioproducts Processing2010，88，： | 1 |
| 16 | Drying char- acteristics of Shiitake mushroom and Jinda chili during vacuum heat pump drying显示文摘 | Artnaseaw A Theerakulpisut S Benjapiyaporn C | 2010 | Food and Bioproducts Processing2010，88，2： | 1 |
| 17 | Mod- eling of a split type air conditioner with interg-reted water heater显示文摘 | Techarungpaisan P Theerakulpisut S Priprem S | 2007 | Energy Conversion and Mana-ge- ment2007，48，4： | 1 |
| 18 | Drying characteristics of shiitake mushroom and jinda chili during vacuum heat pump drying显示文摘 | Artnaseaw A Theerakulpisut S Benjapiyaporn C | 2010 | Food and Bioproducts Processing2010，88，： | 1 |
| 19 | Drying characteristics of Shiitake mushroom and Jinda chili during vacuum heat pump drying 显示文摘 | ARTNASEAW A THEERAKULPISUT S BENJAPIYAPO- RN C | 2010 | Food and Bioproducts Processing2010，88，： | 1 |
| 20 | Drying charactersfics of Shiitake mushroom and Jinda chili during vacuum heat pump drying 显示文摘 | Armaseaw A Theerakulpisut S Benjapiyapom C | 2010 | Food and Bioproducts Processing2010，88，23： | 1 |
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