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| 1 | Helicobacter pylori infection: Host immune response, implications on gene expression and microRNAs显示文摘Helicobacter pylori(H. pylori) infection is the most common bacterial infection worldwide. Persistent infection of the gastric mucosa leads to inflammatory processes and may remain silent for decades or progress causing more severe diseases, such as gastric adenocarcinoma. The clinical consequences of H. pylori infection are determined by multiple factors, including host genetic predisposition, gene regulation, environmental factors and heterogeneity of H. pylori virulence factors. After decades of studies of this successful relationship between pathogen and human host, various mechanisms have been elucidated. In this review, we have made an introduction on H. pylori infection and its virulence factors, and focused mainly on modulation of host immune response triggered by bacteria, changes in the pattern of gene expression in H. pylori-infected gastric mucosa, with activation of gene transcription involved in defense mechanisms, inflammatory and immunological response, cell proliferation and apoptosis. We also highlighted the role of bacteria eradication on gene expression levels. In addition, we addressed the recent involvement of different microRNAs in precancerous lesions, gastric cancer, and inflammatory processes induced by bacteria. New discoveries in this field may allow a better understanding of the role of major factors involved in the pathogenic mechanisms of H. pylori. | Aline Cristina Targa Cadamuro Ana Flávia Teixeira Rossi Nathália Maciel Maniezzo Ana Elizabete Silva | 2014 | World Journal of Gastroenterology2014，20，6： | 16 |
| 2 | TLR2 and TLR4 polymorphisms influence m RNA and protein expression in colorectal cancer显示文摘AIM: To evaluate the effect of promoter region polymorphisms of toll-like receptor(TLR)2-196 to-174 del and TLR4-1607T/C(rs10759932) on m RNA and protein expression in tumor tissue and of TLR4+896A/G(rs4986790) on colorectal cancer(CRC) risk.METHODS: The TLR2-196 to-174 del polymorphism was investigated using allele-specific polymerase chain reaction(PCR) and the TLR4-1607T/C and TLR4+896A/G by PCR-restriction fragment length p o l y m o r p h i s m( R F L P). W e g e n o t y p e d 4 3 4 D N A samples from 194 CRC patients and 240 healthy individuals. The m RNA relative quantification(RQ) was performed in 40 tumor tissue samples by quantitative PCR Taq Man assay, using specific probes for TLR2 and TLR4 genes, and ACTB and GAPDH reference geneswere used as endogenous controls. Protein expression was analyzed by immunohistochemistry with specific primary antibodies.RESULTS: No association was found for TLR4-1607T/C and TLR4+896A/G by three statistical models(logadditive, dominant and recessive). However, based on dominant and log-additive models, the polymorphic variant TLR2-196 to-174 del was associated with increased CRC risk [dominant: odds ratio(OR) = 1.72, 95%CI: 1.03-2.89; P = 0.038 and log-additive: OR =1.59, 95%CI: 1.02-2.48; P = 0.039]. TLR2 m RNA expression was increased in tumor tissue(RQ = 2.36) when compared to adjacent normal tissue(RQ = 1; P < 0.0001), whereas the TLR4 m RNA showed a basal expression(RQ = 0.74 vs RQ = 1, P = 0.452). Immunohistochemistry analysis of TLR2 and TLR4 protein expression was concordant with the findings of m RNA expression. In addition, the TLR2-196 to-174 del variant carriers showed m RNA relative expression 2.19 times higher than wild-genotype carriers. The TLR2 protein expression was also higher for the TLR2-196 to-174 del variant carriers [117 ± 10 arbitrary unit(a.u.) vs 95 ± 4 a.u., P = 0.03]. However, for the TLR4-1607T/C polymorphism no significant difference was found for both m RNA(P = 0.56) and protein expression(P = 0.26).CONCLUSION: Our findings suggest that TLR2-196 to-174 del polymorphism increases TLR2 m RNA expression and is associated with higher CRC risk, indicating an important role in CRC genetic susceptibility. | Marcela Alcantara Proenca Juliana Garcia de Oliveira Aline Cristina Targa Cadamuro Maysa Succi Joao Gomes Netinho Eny Maria Goloni-Bertolo érika Cristina Pavarino Ana Elizabete Silva | 2015 | World Journal of Gastroenterology2015，21，25： | 9 |
| 3 | Noninvasive methods for prediction of esophageal varices in pediatric patients with portal hypertension显示文摘AIM: To evaluate clinical and laboratory parameters for prediction of bleeding from esophageal varices (EV) in children with portal hypertension. METHODS: Retrospective study of 103 children (mean age: 10.1 ± 7.7 years), 95.1% with intrahepatic portal hypertension. All patients had no history of bleeding and underwent esophagogastroduodenoscopy for EV screening. We recorded variceal size (F1, F2 and F3), red-color signs and portal gastropathy, according to the Japanese Research Society for Portal Hypertension classification. Patients were classified into two groups: with and without EV. Seven noninvasive markers were evaluated as potential predictors of EV: (1) platelet count; (2) spleen size z score, expressed as a standard deviation score relative to normal values for age; (3)platelet count to spleen size z score ratio; (4) platelets count to spleen size (cm) ratio; (5) the clinical prediction rule (CPR); (6) the aspartate aminotransferase to platelet ratio index (APRI); and (7) the risk score. RESULTS: Seventy-one children had EV on first endoscopy. On univariate analysis, spleen size, platelets, CPR, risk score, APRI, and platelet count to spleen size z score ratio showed significant associations. The best noninvasive predictors of EV were platelet count [area under the receiver operating characteristic curve (AUROC) 0.82; 95%CI: 0.73-0.91], platelet: spleen size z score (AUROC 0.78; 95%CI: 0.67-0.88), CPR (AUROC 0.77; 95%CI: 0.64-0.89), and risk score (AUROC 0.77; 95%CI: 0.66-0.88). A logistic regression model was applied with EV as the dependent variable and corrected by albumin, bilirubin and spleen size z score. Children with a CPR < 114 were 20.7-fold more likely to have EV compared to children with CPR > 114. A risk score > -1.2 increased the likelihood of EV (odds ratio 7.47; 95%CI: 2.06-26.99). CONCLUSION: Children with portal hypertension with a CPR below 114 and a risk score greater than -1.2 are more likely to have present EV. Therefore, these two tests can be helpful in selecting children for endoscopy. | Marina Rossato Adami Cristina Targa Ferreira Carlos Oscar Kieling Vania Hirakata Sandra Maria Gonalves Vieira | 2013 | World Journal of Gastroenterology2013，19，13： | 4 |
| 4 | 在嗜曙红的食道炎的管理的内视镜检查法的贡献显示文摘 Eosinophilic esophagitis (EoE) is a clinicopathological entity characterized by a set of symptoms similar to gastroesophageal reflux disease and eosinophilic infi ltration of the esophageal epithelium. EoE is an emerging worldwide disease as documented in many countries. Recent reports indicate that EoE is increasingly diagnosed in both pediatric and adult patients although the epidemiology of this new disease entity remains unclear. It is unclear whether EoE is a new disease or a new classification of an old esophageal disorder. Esophagogastroduodenoscopy (EGD) and biopsies with histological examination of esophageal mucosa are required to establish the diagnosis of EoE, verify response to therapy, assess disease remission, document and dilate strictures and evaluate symptom recurrence of EoE. Repeated endoscopies with biopsies are necessary for monitoring of disease progression and treatment effi cacy. EGD has a fundamental role in the diagnosis and management of EoE, forming an essential part of the investigation and follow-up of this condition. EoE is now considered a systemic disorder and not only a local condition with an important immunological back-ground. One of the aims of research in EoE is to study non-invasive markers, such as immune indicators found in plasma, that correlate with local presence of EoE in esophageal tissues. Studies over the next few years will provide new information about diagnosis, pathogenesis, endoscopic/histologic criteria, non-invasive markers, novel and more eff icacious treatments, as well as establishing natural history. Randomized clinical trials are urgently called for to inform non-invasive diagnostic tests, hallmarks of natural history and more eff icacious treatment approaches for patients with EoE. The collaboration between pediatric and adult clinical and experimental studies will be paramount in the understanding and management of this disease. | Cristina Targa Ferreira Helena AS Goldani | 2012 | World Journal of Gastrointestinal Endoscopy2012，4，8： | 3 |
| 5 | Does Parkinsonμs Disease and Type-2 Diabetes Mellitus Present Common Pathophysiological Mechanisms and Treatments?显示文摘 | Marcelo M.S. Lima Adriano D.S. Targa Ana C.D. Noseda Lais S. Rodrigues Ana Marcia Delattre Fabiola V. dos Santos Mariana H. Fortes Maira J. Maturana Anete C. Ferraz | 2014 | CNS & Neurological Disorders - Drug Targets2014，，3： | 2 |
| 6 | Value of accessibility to Bogata's bus rapid transit system显示文摘 | Rodriguez D Targa F | | 0，，05： | 1 |
| 7 | Enzyme-Linked Immunosorbent Assay (ELISA) based on superparamagnetic nanoparticles for aflatoxin M1 detection显示文摘 | RADOI A TARGA M PRIETO-SIMON B | 2008 | Talanta2008，77，1： | 1 |
| 8 | Dysmorphic neurons in patients with temporal lobe epilepsy 显示文摘 | da Silva AV Houzel JC Targas Yacubian EM | 2006 | Brain Research2006，1072，： | 1 |
| 9 | Value of Accessibility to Bogotá's Bus Rapid Transit System显示文摘 | Rodríquez D Targa F | 2004 | Transport Reviews2004，24，5： | 1 |
| 10 | 长期交通相关空气污染暴露与亚临床动脉粥样硬化的关联:REGICOR研究显示文摘[背景]长期空气污染暴露对动脉粥样硬化形成慢性过程影响的流行病学证据并不多。[目的]通过测量颈动脉内膜中层厚度(IMT)和踝臂指数(ABI),探讨长期交通相关空气污染暴露与亚临床动脉粥样硬化的关联。[方法]采用REGICOR(RegistreGironidelCor:赫罗纳心脏病注册)研究中2780名参加者复检期间(2007--2010年)的数据进行横断面分析,REGICOR是在西班牙赫罗纳进行的、以人群为基础的前瞻性队列研究。 | Marcela Rivera Xavier Basagaa Inmaculada Aguilera Maria Foraster David Agis Eric de Groot Laura Perez Michelle A.Mendez Laura Bouso Jaume Targa Rafael Ramos Joan Sala Jaume Marrugat Roberto Elosua Nino Künzli | 2013 | 环境与职业医学2013，30，5： | 1 |
| 11 | Enzyme-Linked im- munosorbent assay (ELISA) based on superparamagnetic nanoparticles for aflatoxin M1 detection显示文摘 | RADOI A TARGA M PRIETO-SIMON B | 2008 | Talanta2008，77，1： | 1 |
| 12 | Enzyme-linked immunosorbent assay (ELISA) based on superparamagnetic nanoparticles for aflatoxin M detection显示文摘 | RADOI A TARGA M PRIETO-SIMON B | 2008 | Talanta2008，77，1： | 1 |
| 13 | Apoptosis in different gastric lesions and gastric cancer:relationship with Helicobacter pylori, overexpression of p53 and aneuploldy显示文摘 | Targa AC Cesar AC Cury PM | 2007 | Genet Mol Res2007，6，3： | 1 |
| 14 | Enzyme-linked immunosorbent assay (ELISA) based on superparamagnetic nanoparticles for aflatoxin M1 detection显示文摘 | RADOI A TARGA M PRIETO-SIMON B | 2008 | Talanata2008，77，1： | 1 |
| 15 | Diagnosis and management of congenital rectourethral fistula in a child with long tubular duplication of the colon and Klippel-Feil syndrome显示文摘 | Eduardo Corrêa Costa Cristina Targa Ferreira Jo?o Luiz Pippi Salle José Carlos Fraga | 2011 | Journal of Pediatric Surgery2011，，11： | 1 |
| 16 | Antinoeiceptive effects of the selective CB2 agonist MT178 in inflammatory and chronic rodent pain models显示文摘 | Vincenzi F Targa M Corcinlo C | 2013 | PAIN2013，154，6： | 1 |
| 17 | Multiple strategies for gene transfer,ex pression,knockdown,and chromatin influence in mammalian cell lines and transgenic animals显示文摘 | Recillas Targa F | 2006 | Mol Biotechnol2006，34，3： | 1 |
| 18 | Apoptosis in different gastric lesions and gastric cancer: relationship with Helicobacter pylori, overexpression of p53 and aneuploidy显示文摘 | Targa AC Cesar AC Cury PM | 2007 | Genet Mol Res2007，6，3： | 1 |
| 19 | Value of Accessibility to Bogota's Bus Rapid Transit System 显示文摘 | Rodriquez D Targa F | 2004 | Transport Reviews2004，，5： | 1 |
| 20 | Enzyme-Linked Immunosorbent Assay (ELISA) based on superparamagnetie nanoparticles for aflatoxin M1 detection显示文摘 | Radoi A Targa M Marry JL | 2008 | Talanta2008，77，1： | 1 |
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