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| 1 | Highly sensitive potentiometric immunosensor for hepatitis B surface antigen diagnosis显示文摘A highly sensitive potentiometric immunosensor for the diagnoses of epidemic dis- eases has been developed by means of self-assembly to immobilize hepatitis B surface antibody (HBsAb) for the detection of hepatitis B surface antigen (HBsAg) as a model. At first, the Nafion containing ?SO3 groups was immobilized on a platinum electrode surface to absorb the ?NH3 ? + groups of antibody molecules via the opposite-charged adsorption technique, in the meantime, hepatitis B surface antibodies were adsorbed onto the surface of Au nanoparticles, then hepatitis B surface antibodies and Au nanopartilces were entrapped into polyvinyl butyral on the surface of Nafion film. The modified procedure was further characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The influence and factors influencing the per- formance of resulting immunosensor were studied in detail. The resulting immunosensor exhib- ited sigmoid curve with log HBsAg concentrations, high sensitivity, wide linear range from 26 to 1280 ng·mL?1 with a detection limit of 3.1 ng·mL?1, rapid potentiometric response (<3 min) and long-term stability (>4 months). Analytical results of clinical samples show that the developed immunoassay is comparable with the enzyme-linked immunosorbent assays (ELISAs) method, implying a promising alternative approach for detecting HBsAg in the clinical diagnosis. | YUAN Ruo TANG Dianping CHAI Yaqin ZHANG Lingyan LIU Yan ZHONG Xia DAI Jianyuan | 2005 | Science China Chemistry2005，48，1： | 2 |
| 2 | Metal sulfide quantum dots-aggregated PAMAM dendrimer for cadmium ion-selective electrode-based immunoassay of alpha-fetoprotein显示文摘An ion-selective electrode(ISE)-based immunoassay has been innovatively designed for the sensitive detection of liver cancer biomarker(alpha-fetoprotein,AFP),using metal sulfide quantum dot(QD)-based nano labels.Cd S QDs-aggregated PAMAM dendrimer(QD-DE)was first synthesized and functionalized with polyclonal rabbit anti-human AFP antibodies.Thereafter,a sandwich immunoreaction was implemented on monoclonal mouse anti-human AFP antibody-coated microplate by using antibody-functionalized QD-DE as the secondary antibody.Accompanying the immunocomplex,subsequent potentiometric detection of cadmium ion dissolved from the QD-DE under acidic condition was conducted on a portable cadmium ion-selective electrode(Cd-ISE).Results revealed that the electrode potential of the Cd-ISE increased with the increment of AFP concentration from 0.1 to 100 ng m L^(-1)at a detection limit(LOD)of 68 pg m L^(-1).The relative standard deviations(RSD)were below9.09%and 10.54%for the intra-and inter-assay,respectively.Additionally,six human serum specimens were determined on CdISE-based immunosensor by using commercial human AFP ELISA kit as the reference,and gave good relationship between two methods.Importantly,Cd-ISE-based immunoassay offers the promise for simple and cost-effective screening of disease-related biomarkers. | Qunfang Li Jing Jin Fangming Lou Dianping Tang | 2018 | Science China Chemistry2018，61，6： | 2 |
| 3 | Nanogold–polyaniline–nanogold microspheres-functionalized molecular tags for sensitive electrochemical immunoassay of thyroid-stimulating hormone显示文摘 | Yuling Cui Huafeng Chen Li Hou Bing Zhang Bingqian Liu Guonan Chen Dianping Tang | 2012 | Analytica Chimica Acta2012，，： | 1 |
| 4 | Electrochemical immuno-bioanalysis for carcinoma antigen 125 based on thionine and gold nanoparticles-modified carbon paste interface显示文摘 | Dianping Tang Ruo Yuan Yaqin Chai | 2006 | Analytica Chimica Acta2006，，2： | 1 |
| 5 | Enzyme-conjugated hybridization chain reaction for magneto-controlled immunoassay of squamous cell carcinoma antigen with pH meter显示文摘A nanoparticle-based potentiometric immunoassay was designed for the sensitive detection of squamous cell carcinoma antigen(SCCA;cervical carcinoma marker) on a portable pH meter coupling enzyme-labeled hybridization chain reaction(HCR) with two alternating hairpin DNA probes for the signal amplification. Initially, a sandwich-type immunoreaction was carried out between anti-SCCA capture antibody-conjugated magnetic bead and detection antibody/initiator strand-coated gold nanoparticle(AuNP). Then, the HCR reaction was readily executed between two glucose oxidase(GOx)-labeled hairpins through the initiator strand to form numerous GOx concatamers on the AuNP via the long nicked double-helix. The concatenated GOx oxidized glucose into gluconic acid and hydrogen peroxide, thus resulting in the pH change of the detection solution on a handheld pH meter. Several labeling protocols including GOx-antibody, GOx-AuNP-antibody and GOx-HCR-AuNP-antibody were investigated for detection of target SCCA, and improved analytical features were obtained with the immune-HCR assay. Under optimum conditions, the immune-HCR assay exhibited good pH responses for the determination of SCCA at a concentration as low as 5.7 pg/mL. Additionally, the immune-HCR assay had good precision and reproducibility, high specificity, and acceptable accuracy for analyzing human serum specimens. | Jiancui Chen Huifeng Xue Qiaoyun Chen Yao Lin Dianping Tang Jinwen Zheng | 2019 | Chinese Chemical Letters2019，30，9： | 1 |
| 6 | Magnetic-Core/Porous-Shell CoFe204/Si02 composite nanoparticles as immobilized affinity supports for clinical immunoassays 显示文摘 | Tang Dianping Yuan Ruo Chai Yaqin | 2007 | Adv Funct Mater2007，3，： | 1 |
| 7 | Electrochemical detection of hepatitis C virus with signal amplification using BamHI endonuclease and horseradish peroxidase- encapsulated nanogold hollow spheres 显示文摘 | Tang Dianping Tang Juan Su Biling | 2011 | Chem Commun2011，47，： | 1 |
| 8 | Magneto-controlled graphene immunosensing platform for simultaneous multiplexed electrochemical immunoassay using distinguishable signal tags显示文摘 | Tang Juan Tang Dianping Niessner Reinhard | 2011 | Anal Chem2011，83，： | 1 |
| 9 | Nanoparticles- based sandwich electrochemical immunoassay for carbohydrate antigen 125 with signal enhancement using enzyme-coated nanometer-sized enzyme-doped silica beads 显示文摘 | Tang Dianping Su Biling Tang Juan | 2010 | Anal Chem2010，82，： | 1 |
| 10 | Hierarchical dendritic gold microstructure-based aptasensor for ultrasensitive electrochemical detection of thrombin using functionalized mesoporous silica nanospheres as signal tags显示文摘 | Juan Tang Dianping Tang Reinhard Niessner Dietmar Knopp Guonan Chen | 2012 | Analytica Chimica Acta2012，，： | 1 |
| 11 | Ultrasensitive electrochemical immunoassay of staphylococcal enterotoxin B in food using enzyme-nanosiliea-doped carbon nanotubes for signal amplification显示文摘 | Tang Dianping Tang Juan Su Biling | 2010 | J Agrie Food Chem2010，58，10： | 1 |
| 12 | Simultaneous determination of five-type hepatitis virus antigens in five minutes using an integrated automatic electrochemical immunosensor array显示文摘 | Tang Dianping Tang Juan Su Biling | 2010 | Biosens Bioelectron2010，25，： | 1 |
| 13 | Magnetocontrolled electrochemical immunosensor for direct detection of squamous cell carcinoma antigen by using serum as supporting electrolyte 显示文摘 | Li Qunfang Tang Dianping Tang Juan | 2011 | Biosens Bioelectron2011，27，： | 1 |
| 14 | Study on the immobilization of anti-IgG on Au-colloid modified gold electrode via potentiometric immunosensor, cyclic voltammetry, and electroehemical impedance techniques显示文摘 | Yingzi Fu Ruo Yuan Dianping Tang | 2005 | Colloids and Surfaces B:Biointerfaces2005，，40： | 1 |
| 15 | Study on the immobilization of anti-IgG on Au-colloid gold electrode via potentiometric immunosesor,cyclic voltammetry,and electrochemical I impedance techniques显示文摘 | Ying Fu Ruo Yuan Dianping Tang | 2005 | Colloids and Surfaces B:Biointerfaces2005，，40： | 1 |
| 16 | Magnetic coreshellFeaO4@Ag nanoparticles coated carbon paste interface for studies earcinoembryonic antigen in clinical imrnunoassay 显示文摘 | Tang Dianping Yuan Ruo Chai Yaqin | 2006 | J Phys Chem B2006，110，11： | 1 |
| 17 | Electron-transfer mediator microbiosensor fabrication based on immobilizing HRP-labeled Au colloids on gold electrode surface by 11-mercaptoundecanoic acid monolayer显示文摘 | Tang Dianping Yuan Ruo Yaqin Chai | 2006 | Electroanalysis2006，18，3： | 1 |
| 18 | Study on the immobilization of anti-IgG on Au-colloid modified gold electrode via potentiometric immunosensor,cyclic voltammetry,and electrochemica l impedance techniques显示文摘 | Yingzi Fu Ruo Yuan Dianping Tang | 2005 | Colloids and Surfaces2005，，： | 1 |
| 19 | Glucose Biosensor Based on Self-Assembled Gold Nanoparticles and Double-Layer 2d-Network (3-Mercap-topropyl)-Tfimethoxysilane Polymer onto Gold Substrate显示文摘 | Ruo Yuan Yaqin Chai Yan Liu Jianyuan Dai Dianping Tang | 2005 | Sensors and Actuators B2005，104，： | 1 |
| 20 | Target-induced structure-switching DNA hairpins for sensitive electrochemical monitoring of mercury (Ⅱ)显示文摘 | ZHUANG Junyang FU Libing TANG Dianping | 2013 | Biosensors and Bioelectronics2013，39，1： | 1 |
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