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| 1 | CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells显示文摘In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2,no specific and effective drugs for treating this disease have been reported until today.Angiotensin-converting enzyme 2(ACE2),a receptor of SARS-CoV-2,mediates the virus infection by binding to spike protein.Although ACE2 is expressed in the lung,kidney,and intestine,its expressing levels are rather low,especially in the lung.Considering the great infectivity of COVID-19,we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection.Here,we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein.The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody,Meplazumab,inhibits SARSCoV-2 amplification.Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells,which can be neutralized by CD147 extracellular fragment.Viral loads are detectable in the lungs of human CD147(hCD147)mice infected with SARS-CoV-2,but not in those of virus-infected wild type mice.Interestingly,virions are observed in lymphocytes of lung tissue from a COVID-19 patient.Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dosedependent manner,which is specifically inhibited by Meplazumab.Furthermore,CD147 mediates virus entering host cells by endocytosis.Together,our study reveals a novel virus entry route,CD147-spike protein,which provides an important target for developing specific and effective drug against COVID-19. | Ke Wang Wei Chen Zheng Zhang Yongqiang Deng Jian-Qi Lian Peng Du Ding Wei Yang Zhang Xiu-Xuan Sun Li Gong Xu Yang Lei He Lei Zhang Zhiwei Yang Jie-Jie Geng Ruo Chen Hai Zhang Bin Wang Yu-Meng Zhu Gang Nan Jian-Li Jiang Ling Li Jiao Wu Peng Lin Wan Huang Liangzhi Xie Zhao-Hui Zheng Kui Zhang Jin-Lin Miao Hong-Yong Cui Min Huang Jun Zhang Ling Fu Xiang-Min Yang Zhongpeng Zhao Shihui Sun Hongjing Gu Zhe Wang Chun-Fu Wang Yacheng Lu Ying-Ying Liu Qing-Yi Wang Huijie Bian Ping Zhu Zhi-Nan Chen | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 9 |
| 2 | Two new prenylflavonoids from Morus nigra L.显示文摘二新 prenylflavonoids, mornigrol E (1 ) 和 mornigrol F (2 ) 从桑属 nigra L 被孤立。他们的结构根据分光镜的方法被阐明,特别 2D-NMR 技术。 | Lei Wang Ting Gong Ruo Yun Chen | 2009 | Chinese Chemical Letters2009，20，12： | 8 |
| 3 | A novel impedance immunosensor based on O-phenylenediamine modified gold electrode to analyze abscisic acid显示文摘An impedance immunosensor based on O-phenylenediamine modified gold electrode for the determination of phytohormone abscisic acid(ABA) was proposed.The operating pH,absorption time,absorption temperature and concentration of anti-ABA antibody were investigated to optimize the analytical performance.The calibration curve for the determination of ABA was obtained from this impedance immunosensor under optimal conditions.The results showed that the detection limit at about 1 ng/mL in the range of 10-5000 ng... | Li, Qian Wang, Ruo Zhong Huang, Zhi Gang Li, He Song Xiao, Lang Tao | 2010 | Chinese Chemical Letters2010，21，4： | 7 |
| 4 | Visfatin Protects Rat Pancreatic β-cells against IFN-γ-Induced Apoptosis through AMPK and ERK1/2 Signaling Pathways显示文摘Objective Interferon-γ(IFN-γ) plays an important role in apoptosis and was shown to increase the risk of diabetes. Visfatin, an adipokine, has anti-diabetic, anti-tumor, and regulating inflammatory properties. In this study we investigated the effect of visfatin on IFN-γ-induced apoptosis in rat pancreatic β-cells. Methods The RINm5F(rat insulinoma cell line) cells exposed to IFN-γ were treated with or without visfatin. The viability and apoptosis of the cells were assessed by using MTT and flow cytometry. The expressions of m RNA and protein were detected by using real-time PCR and western blot analysis. Results The exposure of RINm5 F cells to IFN-γ for 48 h led to increased apoptosis percentage of the cells. Visfatin pretreatment significantly increased the cell viability and reduced the cell apoptosis induced by IFN-γ. IFN-γ-induced increase in expression of p53 m RNA and cytochrome c protein, decrease in m RNA and protein levels of anti-apoptotic protein Bcl-2 were attenuated by visfatin pretreatment. Visfatin also increased AMPK and ERK1/2 phosphorylation and the anti-apoptotic action of visfatin was attenuated by the AMPK and ERK1/2 inhibitor. Conclusion These results suggested that visfatin protected pancreatic islet cells against IFN-γ-induced apoptosis via mitochondria-dependent apoptotic pathway. The anti-apoptotic action of visfatin is mediated by activation of AMPK and ERK1/2 signaling molecules. | XIANG Ruo Lan MEI Mei SU Yun Chao LI Li WANG Jin Yu WU Li Ling | 2015 | Biomedical and Environmental Sciences2015，28，3： | 7 |
| 5 | Sonochemical effect of a bifrequency irradiation显示文摘The sonochemical effect of a bifrequency orthogonal ultrasonic irradiation has been studied by using two different detecting methods. It has been shown that the sonochemical yield produced by the bifrequency irradiation appears to be much more than the sum of the yields produced separately by the two irradiations. The bifrequency irradiation, therefore, can be expected to exert a great influence on the development of sonohemical | ZHU Changping, FENG Ruo & ZHAO Yiyun Department of Physics. Jingzhou Normal College, Jingzhou 434100, China State Key Laboratory of Modern Acoustics, Nanjing University, Nanjing 210093, China Chemistry Department of Yunnan University, Kunming 650091, Ch | 2000 | Chinese Science Bulletin2000，45，2： | 7 |
| 6 | Albuterol in the treatment of acute respiratory distress syndrome: A meta-analysis of randomized controlled trials显示文摘BACKGROUND: This meta-analysis of randomized controlled trials aimed to systematically evaluate the value of albuterol in the treatment of patients with acute respiratory distress syndrome(ARDS).DATA SOURCES: Randomized controlled trials on albuterol treatment of ARDS from its inception to October 2014 were searched systematically. The databases searched included: Pub Med, Ovid EMBASE, Ovid Cochrane, CNKI, WANFANG and VIP. The trials were screened according to the pre-designed inclusion and exclusion criteria. We performed a systematic review and meta-analysis of the randomized controlled trials(RCTs) on albuterol treatment, attempting to improve outcomes, i.e. lowering the 28-day mortality and ventilator-free days.RESULTS: Three RCTs involving 646 patients met the inclusion criteria. There was no significant decrease in the 28-day mortality(risk difference=0.09; P=0.07, P for heterogeneity=0.22, I2=33%). The ventilator-free days and organ failure-free days were significantly lower in the patients who received albuterol(mean difference=–2.20; P<0.001, P for heterogeneity=0.49, I2=0% and mean difference=–1.71, P<0.001, P for heterogeneity=0.60, I2=0%).CONCLUSIONS: Current evidences indicate that treatment with albuterol in the early course of ARDS was not effective in increasing the survival, but significantly decreasing the ventilator-free days and organ failure-free days. Owing to the limited number of included trails, strong recommendations cannot be made. | Ruo Wu Shi-yun Lin Hui-min Zhao | 2015 | World Journal of Emergency Medicine2015，6，3： | 6 |
| 7 | CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta,alpha,beta,and gamma显示文摘SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape,compromising the effectiveness of existing vaccines and neutralizing antibodies.An in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 variants.Here,we identified CD147 as a universal receptor for SARS-CoV-2 and its variants.Meanwhile,Meplazeumab,a humanized anti-CD147 antibody,could block cellular entry of SARS-CoV-2 and its variants-alpha,beta,gamma,and delta,with inhibition rates of 68.7,75.7,52.1,52.1,and 62.3%at 60μg/ml,respectively.Furthermore,humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants,alpha and beta.When infected,these mice developed exudative alveolar pneumonia,featured by immune responses involving alveoli-infiltrated macrophages,neutrophils,and lymphocytes and activation of IL-17 signaling pathway.Mechanistically,we proposed that severe COVID-19-related cytokine storm is induced by a'spike protein-CD147-CyPA signaling axis':Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway,which further induced expression of cyclophilin A(CyPA);CyPA reciprocally bound to CD147 and triggered MAPK pathway.Consequently,the MAPK pathway regulated the expression of cytokines and chemokines,which promoted the development of cytokine storm.Importantly,Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its variants.Therefore,our findings provided a new perspective for severe COVID-19-related pathogenesis.Furthermore,the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment. | Jiejie Geng Liang Chen Yufeng Yuan Ke Wang Youchun Wang Chuan Qin Guizhen Wu Ruo Chen Zheng Zhang Ding Wei Peng Du Jun Zhang Peng Lin Kui Zhang Yongqiang Deng Ke Xu Jiangning Liu Xiuxuan Sun Ting Guo Xu Yang Jiao Wu Jianli Jiang Ling Li Kun Zhang Zhe Wang Jing Zhang Qingguo Yan Hua Zhu Zhaohui Zheng Jinlin Miao Xianghui Fu Fengfan Yang Xiaochun Chen Hao Tang Yang Zhang Ying Shi Yumeng Zhu Zhuo Pei Fei Huo Xue Liang Yatao Wang Qingyi Wang Wen Xie Yirong Li Mingyan Shi Huijie Bian Ping Zhu Zhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 6 |
| 8 | A New Isoprenylated flavonol from the Leaves of Broussonetia kazinoki显示文摘A new isoprenylated flavonol, named broussonol A (1) was isolated from the leaves of Broussonetia kazinoki Sieb. The structure of broussonol A was characterized by chemical and spectral methods. | Pei Cheng ZHANG, Si WANG, Ying Hong WANG, Ruo Yun CHEN, De Quan YU* Institute of Materia Medica, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050 | 2001 | Chinese Chemical Letters2001，12，2： | 5 |
| 9 | 酶解乳蛋白对初生仔猪小肠组织学结构及隐窝细胞增殖的影响(英文)显示文摘INTRODUCTIONTheintestineasthemajorinterfacebetweenanorganismanditsnutritionalenvironmentplaysacriticalroleinpostnataldevelopm... | Tian Wang 1,Ruo Jun Xu 2, Yuna Qiang Zhang 3, Chong Tian Zheng 1 and Jian Cheng Zhang 4 1 Department of Animal Nutrition and Feed, Nanjing Agricultural University,210095 2 Department of Zoology, The University of Hong Kong 3 Department | 1999 | 中国组织化学与细胞化学杂志1999，8，2： | 5 |
| 10 | STRUCTURE ELUCIDATION OF LIGUSTILONE FROM LIGUSTICUM SINENSIS OLIV显示文摘STRUCTUREELUCIDATIONOFLIGUSTILONEFROMLIGUSTICUMSINENSISOLIV￥DeQuanYU;RuoYunCHEN;andFengZhiXIE.(InstituteofMateriaMedica,Chine... | De Quan YU Ruo Yun CHEN and Feng Zhi XIE.(Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing,100050) | 1995 | Chinese Chemical Letters1995，6，5： | 4 |
| 11 | Parity-decomposition and moment analysis for stationary Wigner equation with inflow boundary conditions显示文摘我们由使用 L 的同等值分解与流入边界条件在一个围住的、一个维的空间领域上学习静止 Wigner 方程。Barletti 和 P. F。Zweifel [运输理论统计员。Phys, 2001, 30 (4-6 ) :507-520 ] 。分解减少一半范围,二点的边界价值问题进二 decoupled 平部分的起始的价值问题和奇怪的部分。没有在零速度附近使用截止近似,我们证明为平部分的起始的价值问题是提出得好的。为奇怪的部分,我们由分析片刻系统在奇怪的 L 2-space 证明解决方案的唯一。一个例子被提供看那怎么使用分析与流入边界条件获得静止 Wigner 方程的答案。 | Ruo LI Tiao LU Zhangpeng SUN | 2017 | Frontiers of Mathematics in China2017，12，4： | 4 |
| 12 | A capacitive sensor based on molecularly imprinted polymers and poly(p-aminobenzene sulfonic acid) film for detection of pazufloxacin mesilate显示文摘A novel capacitive sensor for pazufloxacin mesilate (pazufloxacin) determination was developed by electropolymerizing p-aminobenzene sulfonic (p-ABSA) and molecularly imprinted polymers (MIPs), which was synthesized through thermal radical copolymerization of metharylic acid (MAA) and ethyl-ene glycol dimethacrylate (EGDMA) in the presence of pazufloxacin template molecules, on the gold electrode surface. Furthermore, 1-dedecanethiol was used to insulate the modified electrode. Alter-nating current (ac) impedance experiments were carried out with a Model IM6e to obtain the capaci-tance responses. Under the optimum conditions, the sensor showed linear capacitance response to pazufloxacin in the range of 5 ng·mL-1 to 5 μg·mL-1 with a relative standard deviation (RSD) 5.3% (n=7) and a detection limit of 1.8 ng·mL-1. The recoveries for different concentration levels of pazufloxacin samples varied from 94.0% to 102.0%. Electrochemical experiments indicated the capacitive sensor exhibited good sensitivity and selectivity and showed excellent parameters of regeneration and stabil-ity. | ZHOU Lu YE GuangRong YUAN Ruo CHAI YaQin CHEN SuMing | 2007 | Science China Chemistry2007，50，4： | 4 |
| 13 | Formation of ZrB2in MgO-C Composite Materials Using In-Situ Synthesis Method显示文摘ZrB2 in MgO-C composite materials obtained by both microwave sintering synthesis and in-situ reaction at lower temperature was investigated. The test result shows that 66.67% of reactant changed into ZrB2, and the other form Al2O3, which could optimize the properties of carbon-containing materials. Therefore, the method of in-situ synthesizing ZrB2 acting as antioxidant in carbon-containing materials is considered as one of best ways to reduce the cost and ZrB2 as refractories is used on a large-scale. | LI Lin HONG Yan ruo SUN Jia-lin HE Zhi-yong PENG Xiao-yan | 2006 | Journal of Iron and Steel Research(International)2006，13，1： | 3 |
| 14 | Operative Blood Loss Independently Predicts Recurrence and Survival After Resection of Hepatocellular Carcinoma显示文摘 | Steven C. Katz Jinru Shia Kui Hin Liau Mithat Gonen Leyo Ruo William R. Jarnagin Yuman Fong Michael I. D?Angelica Leslie H. Blumgart Ronald P. DeMatteo | 2009 | Annals of Surgery2009，，4： | 3 |
| 15 | Determination of carcinoembryonic antigen using a novel amperometric enzyme-electrode based on layer-by-layer assembly of gold nanoparticles and thionine显示文摘Electrochemical sensing of carcinoembryonic antigen(CEA)on a gold electrode modified by the se- quential incorporation of the mediator,thionine(Thi),and gold nanoparticles(nano-Au),through co- valent linkage and electrostatic interactions onto a self-assembled monolayer configuration is de- scribed in this paper.The enzyme,horseradish peroxidase(HRP),was employed to block the possible remaining active sites of the nano-Au monolayer,avoid the non-specific adsorption,instead of bovine serum albumin(BSA),and amplify the response of the antigen-antibody reaction.Electrochemical ex- periments indicated highly efficient electron transfer by the imbedded Thi mediator and adsorbed nano-Au.The HRP kept its activity after immobilization,and the studied electrode showed sensitive response to CEA and high stability during a long period of storage.The working range for the system was 2.5 to 80.0 ng/mL with a detection limit of 0.90 ng/mL.The model membrane system in this work is a potential biosensor for mimicking the other immunosensor and enzyme sensor. | YUAN Ruo ZHUO Ying CHAI YaQin ZHANG Ying SUN AiLi | 2007 | Science China Chemistry2007，50，1： | 3 |
| 16 | Novel guanidinium-based ionic liquids as stationary phases for capillary gas chromatography显示文摘The present study describes guanidinium-based ionic liquids(GBILs) as stationary phases for capillary gas chromatography (CGC) and to the best of our knowledge,no related reports are available up to now.In this study,a hexaalkylguanidinium ionic liquid(DOTMG-NTf_2) was synthesized and coated statically onto capillary columns.Selectivity of the stationary phase was evaluated by separating Grob test mixture,test mixture,alcohols mixture,and fatty acid methyl esters mixture,and thermal stability was investigated as well.The present study demonstrates that GBILs as CGC stationary phases exhibit satisfactory selectivity and thermal stability and have a great potential as new candidates for CGC stationary phases. | Li Zhen Qiao Kai Lu Mei Ling Qi Ruo Nong Fu | 2010 | Chinese Chemical Letters2010，21，9： | 3 |
| 17 | Synthesis and Characterisation of Water-Soluble Carboxymethyl-Cyclodextrin Polymer as Capillary Electrophoresis Chiral Selector显示文摘The water-soluble carboxymethyl-cyclodextrin polymer (CM-CD polymer) was synthesized and used as capillary electrophoresis chiral selector. Verrapamil and thiopentorusodium were well separated using CM-CD polymer as chiral selector. | Xue Yan SHI Rui Fang FAN Yue Qin ZHANG Jun Ling GU Ruo Nong FU( College of Chemical Engineering and Material Science Beijing Institute of Technology, Beijing 100081) | 2000 | Chinese Chemical Letters2000，11，1： | 3 |
| 18 | Amperometric Determination of lndole-3-acetic Acid Based on Platinum Nanowires and Carbon Nanotubes显示文摘Platinum nanowire (PtNW) can be grown by electrodeposition in polycarbonate membrane, with the average diameter of the nanowires about 250 nm. The PtNW and multiwalled carbon nanotubes (CNT) are then dispersed into chitosan (CHIT) solution. The resulting PtNW-CNT-CHIT material brings new capabilities for electrochemical devices by using the synergistic action of the electrocatalytic activity of PtNW and CNT. By dropping the PtNW-CNT-CHIT film onto the glassy carbon (GC) electrode surface, and after evaporation an amperometric sensor for the determination of indole-3-acetic acid (IAA) was developed. The oxidation current of IAA increased significantly at the PtNW-CNT-CHIT film coated GC electrode, in contrast to that at the CNT-CHIT modified GC. The linear response of the sensor is from 50 ng/ml to 50 μg/ml with a detection limit of 25 ng/mL. | Ruo Zhong WANG Lang Tao XIAO Ming Hui YANG Jun Hui DING Feng Li QU Guo Li-SHEN | 2006 | Chinese Chemical Letters2006，17，12： | 3 |
| 19 | Glucose oxidase as a blocking agent-based signal amplification strategy for the fabrication of label-free amperometric immunosensors显示文摘An effective electrochemical signal amplification strategy based on enzyme membrane modification and redox probe immobilization was proposed to construct an amperometric immunosensor.L-cysteine@ferrocene functionalized chitosan,which possessed not only efficient redox-activity but also excellent film-forming ability,was coated on the bare glass carbon electrode. Moreover,the thiol groups(SH)in the ferrocenyl compound were used for gold nanoparticles immobilization via the strong bonding interaction,which could further be utilized for the immobilization of antibody biomolecules with well-retained bioactivities.Finally,glucose oxidase(GOD)as the enzyme membrane was employed to block the possible remaining active sites and avoid the nonspecific adsorption.With the excellent electrocatalytic properties of GOD towards glucose,the amplification of antigen-antibody interaction and the enhanced sensitivity could be achieved.Under the optimal conditions,the linear range of the proposed immunosensor for the determination of carcinoembryonic antigen(CEA)was from 0.05 to 100 ng/mL with a detection limit of 0.02 ng/mL(S/N=3).Moreover,the immunosensor exhibited good selectivity,stability and reproducibility, which provided a promising potential for clinical immunoassay. | SONG ZhongJu YUAN Ruo CHAI YaQin CHE Xin LV Ping | 2011 | Science China Chemistry2011，54，3： | 3 |
| 20 | Diverse coagulopathies in a rabbit model with different abdominal injuries显示文摘BACKGROUND: Although coagulopathy can be very common in severe traumatic shock patients, the exact incidence and mechanism remain unclear. In this study, a traumatic shock rabbit model with special abdomen injuries was developed and evaluated by examining indicators of clotting and fi brinolysis.METHODS: Forty New Zealand white rabbits were randomly divided into four groups: group 1(sham), group 2(hemorrhage), group 3(hemorrhage-liver injury), and group 4(hemorrhage-liver injury/intestinal injury-peritonitis). Coagulation was detected by thromboelastography before trauma(T0), at 1 hour(T1) and 4 hours(T2) after trauma.RESULTS: Rabbits that suffered from hemorrhage alone did not differ in coagulation capacity compared with the sham group. The clot initiations(R times) of group 3 at T1 and T2 were both shorter than those of groups 1, 2, and 4(P<0.05). In group 4, clot strength was decreased at T1 and T2 compared with those in groups 1, 2, and 3(P<0.05), whereas the R time and clot polymerization were increased at T2(P<0.05). The clotting angle signifi cantly decreased in group 4 compared with groups 2 and 3 at T2(P<0.05).CONCLUSION: This study suggests that different abdominal traumatic shock show diverse coagulopathy in the early phase. Isolated hemorrhagic shock shows no obvious effect on coagulation. In contrast, blunt hepatic injury with hemorrhage shows hypercoagulability, whereas blunt hepatic injury with hemorrhage coupled with peritonitis caused by a ruptured intestine shows a tendency toward hypocoagulability. | Ruo Wu Luo-gen Peng Hui-min Zhao | 2017 | World Journal of Emergency Medicine2017，8，2： | 3 |
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