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| 1 | Fully automated sample treatment method for high throughput proteome analysis显示文摘The bottom-up strategy for proteome analysis typically employs a multistep sample preparation workflow that suffers from being time-consuming and sample loss or contamination caused by the off-line manual operation.Herein,we developed a hollow fibre membrane(HFM)-aided fully automated sample treatment(FAST)method.Due to the confinement effects of HFMs and the immobilized enzymatic reactor,the proteome samples could be denatured,reduced,desalted and digested within 8–20 min via the one-stop service.This method also showed superiority in trace sample analysis.In one and half hours,we could identify about 1,600 protein groups for 500 HeLa cells as the starting materials,1.5–8 times more than those obtained by previously reported methods.Through the on-line combination of FAST with nano-liquid chromatography-electrospray ionization tandem mass spectrometry(nanoLC-ESI-MS/MS),we further established a fully integrated platform for label-free quantification of proteome with high reproducibility and precision.Collectively,FAST presented here represents a major advance in the high throughput sample treatment and quantitative analysis of proteomes. | Huiming Yuan Zhongpeng Dai Xiaodan Zhang Baofeng Zhao Hongwei Chu Lihua Zhang Yukui Zhang | 2021 | Science China Chemistry2021，64，2： | 0 |
| 2 | 基于胶体金侧向层析原理的大米中痕量镉现场快速检测方法显示文摘目的:建立一种可实现对大米中镉元素现场快速检测的胶体金试纸条检测方法。方法:基于侧向免疫层析技术,对胶体金试纸条一系列影响检测性能的参数进行系统优化。结果:所制备的胶体金试纸条对大米中镉元素的检测达到了可视化检测限为10 ng/g的水平。在实际应用中,此方法样品前处理过程操作简单,低浓度酸直接提取并稀释后即可用于试纸条检测。结论:胶体金试纸条快速检测方法适用于大米镉的现场快速筛检。 | 王冉冉 姚丽 姚帮本 陈伟 陈赵然 | 2023 | 食品与机械2023，39，8： | 0 |
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