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| 1 | miR-106b-25/miR-17-92 clusters: Polycistrons with oncogenic roles in hepatocellular carcinoma显示文摘MicroRNAs are small endogenously expressed RNA molecules which are involved in the process of silencing gene expression through translational regulation.The polycistronic miR-17-92 cluster is the first microRNA cluster shown to play a role in tumorigenesis.It has two other paralogs in the human genome,the miR-106b-25 cluster and the miR-106a-363 cluster.Collectively,the microRNAs encoded by these clusters can be further grouped based on the seed sequences into four families,namely the miR-17,the miR-92,the miR-18and the miR-19 families.Over-expression of the miR-106b-25 and miR-17-92 clusters has been reported not only during the development of cirrhosis but also subsequently during the development of hepatocellular carcinoma.Members of these clusters have also been shown to affect the replication of hepatitis B and hepatitis C viruses.Various targets of these microRNAs have been identified,and these targets are involved in tumor growth,cell survival and metastasis.In this review,we first describe the regulation of these clusters by c-Myc and E2F1,and how the members of these clusters inturn regulate E2F1 expression forming an auto-regulatory loop.In addition,the roles of the various members of the clusters in affecting relevant target gene expression in the pathogenesis of hepatocellular carcinoma will also be discussed. | Weiqi Tan Yang Li Seng-Gee Lim Theresa MC Tan | 2014 | World Journal of Gastroenterology2014，20，20： | 17 |
| 2 | Expression of perforin and granzyme B mRNA in judgement of immunosuppressive effect in rat liver transplantation显示文摘AIM To explore the expression of perform andgranzyme B genes mRNA to judge the effect ofimmunosuppression in acute rejection of livertransplantation.METHODS The expression of perform andgranzyme B genes mRNA was examined byreverse transcriptase-polymerase chain reaction(RT--PCR) in hamster to rat liver grafts under theimmunosuppression of cyclosporine or/andsplenectomy. Histological findings were studiedcomparatively.RESULTS Cyclosporine could obviouslydecrease the cellular infiltration, and completelyrepress the expression of mRNA for perform andgranzyme B, but could not change severehepatocyte necrosis and hemorrhage.SPlenectomy could significantly lightenhepatocyte necrosis, and completely eliminatehemorrhage, but not atfect the cellularinfiltration and the expression of perform andgranzyme B genes mRNA. Cyclosporine orsplenectomy alone could not prolong theSurvival time, however, their combination couldcompletely repress the rejection of liver grafts.The survival time of animals were significantlyprolonged (37.1 days ). The architecture ofhepatic lobules was preserved. There was slightcellular infiltration in the portal tracts and noexpression of perform and granzyme B genesmRNA could be seen in three weeks aftertransplantation.CONCLUSION Perform and granzyme B genesare valuable in judging the effect ofimmunosuppression in liver transplantation. | Zhang SG Wu MC Tan JW Chen H Yang JM Qian QJ | 1999 | World Journal of Gastroenterology1999，5，3： | 15 |
| 3 | Up-regulation of micro RNA-210 inhibits proliferation of hepatocellular carcinoma cells by targeting YES1显示文摘AIM: To determine the expression of micro RNA-210(mi R-210) in hepatocellular carcinoma(HCC) and to examine its role using HCC cells.METHODS: The expression of mi R-210 was determined in 21 pairs of HCC samples and the corresponding surrounding non-tumor tissues. The effects of mi R-210 on proliferation and cell cycle progression were examined using Hep G2 and Hu H7 cells. Overexpression and inhibition of mi R-210 was achieved by transfection of the cells with mi R-210 mimic or inhibitor. Luciferase reporter constructs were used to identify the mi R-210 interacting site on Yes1. Yes1 expression was examined after mi R-210 transfection,as well as in the HCC samples.RESULTS: mi R-210 was significantly up-regulated by 3.4 fold(P < 0.01) in the tumor samples. The over-expression of mi R-210 significantly reduced cell proliferation compared to the mock-treated cells(68.9% ± 7.4% and 53.6% ± 5.0%,P < 0.05 for the Hep G2 and Hu H7 cells respectively). Analysis of the Hu H7 cells transfected with mi R-210 mimic by flow cytometry showed that the cells took a longer time to reach the G2/M phase. The interaction between mi R-210 and the 3'UTR of the Yes1 transcript was confirmed using a luciferase reporter assay. Over-expression of mi R-210 reduced the expression of Yes1 protein in both Hu H7 and Hep G2 cells. Tumors with a greater than fourfold increase in the expression of mi R-210 showed consistently lower expressions of Yes1 in the tumors.In nocodazole-treated cells with a significant G2/M cell population,Yes1 protein was significantly reduced and pre-inhibition of mi R-210 in Hu H7 cells was able to prevent the reduction of Yes1 protein expression. Knock-down of Yes1 by si RNA also led to reduced cell proliferation(70.8% ± 7.5%,P < 0.05 in the Hu H7 cells).CONCLUSION: Up-regulation of mi R-210 inhibits cell proliferation. Yes1 is a target of mi R-210 and affects cell proliferation in HCC. | Weiqi Tan Seng-Gee Lim Theresa MC Tan | 2015 | World Journal of Gastroenterology2015，21，46： | 4 |
| 4 | Laparoscopic nephroureterctomy with bladder-cuff resection techniques and outcomes显示文摘 | Tan BJ Ost MC Lee BE | 2005 | Endourol2005，19，6： | 1 |
| 5 | A systematic review of post-extractional alveolar hard and soft tissue dimensional changes in humans 显示文摘 | Tan WL Wong TL Wong MC | 2012 | Clin Oral Implants Res2012，23，5： | 1 |
| 6 | The association of cardiovascular disease with impaired health-related quality of life among patients with type 2 diabetes mellitus 显示文摘 | Tan MC Ng OC Wong TW | 2014 | Singapore Med J2014，55，4： | 1 |
| 7 | Factors affecting outcome of one-stageanteriorhypospadiasrepair, analysisof422 cases 显示文摘 | Uygur MC Unal D Tan MO | 2002 | Pediatr Surg Int2002，18，23： | 1 |
| 8 | Accuracy of staging as a predictor for recurrence after liver transplantation for hepatocellular carcinoma显示文摘 | Shah SA Tan JC Mc Gilvray ID | 2006 | Transplantation2006，81，： | 1 |
| 9 | Yeast cell ad- hesion molecules have functional amyloid-forming se- quences显示文摘 | Ramsook CB Tan C Garcia MC | 2010 | Eukaryot Cell2010，9，3： | 1 |
| 10 | The effect of central retinal venous pressure in patients with central retinal vein occlusion and a high mean area of nonperfusion显示文摘 | Mc Allister IL Tan MH Smithies LA | 2014 | Ophthalmology2014，121，11： | 1 |
| 11 | Genetic evidence for convergence of c Kit and alph 4 integrin mediated signals on class Ⅰ API3 kinase and the rac pathway in regulating integrin in directed migrtion in mast cell显示文摘 | Tan B L Yazicioglu M N Mc Carthy J | 2003 | Blood2003，101，12： | 1 |
| 12 | Disruption of CCRS-dependent homing of regulatory T cells inhibits tumor growth in a murine model of pancreatic cancer 显示文摘 | Tan MC Goedegebuure PS Belt BA | 2009 | J Immunol2009，182，3： | 1 |
| 13 | Purification and properties of the L-amino acid oxidase from Malayan pit viper (Calloselasma rhodostoma) venom显示文摘 | Ponnudurai G Chung MC Tan NH | 1994 | Arch Bionchem Biophs1994，313，2： | 1 |
| 14 | Prehospital delay after acute stroke in Kaohsiung Taiwan显示文摘 | Chang KC Tseng MC Tan TY | 2004 | Stroke2004，35，3： | 1 |
| 15 | Disruption of CCRS- dependent homing of regulatory T cells inhibits tumor growth in a rnurine model of pancreatic cancer显示文摘 | Tan MC Goedegebuure PS Belt BA | 2009 | J Immunol2009，182，3： | 1 |
| 16 | BpeAB-OprB,amultidrug efflux pump in Burkholderia pseudomallei显示文摘 | Chan YY Tan MC Ong YM | 2004 | Antimicrob Agent Chemother2004，48，4： | 1 |
| 17 | Problem-based learning in medical、education:the Singapore hybrid显示文摘 | Tan CH | 2001 | Ann Acad Med Singapore2001，30，4： | 1 |
| 18 | Urological laparoscopy : basic physiological considerations and immunological consequences 显示文摘 | Ost MC Tan B J Lee BR | 2005 | J Urol2005，174，41： | 1 |
| 19 | Factors affecting outcome of one-stage anterior hypospadias repair:analysis of 422cases显示文摘 | Uygur MC Unal D Tan MO | | 0，，2： | 1 |
| 20 | Subcellular fractionation methods and strategies for proteomics 显示文摘 | Lee YH Tan HT Chang MC | 2010 | Proteonfics2010，10，22： | 1 |
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