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| 1 | Reduced expression ofβ-catenin inhibitor Chibby in colon carcinoma cell lines显示文摘AIM: To analyse the Chibby expression and its function in colon carcinoma cell lines and colorectal carcinoma (CRC). METHODS: Chibby expression levels were investigated by quantitative RT-PCR in a panel of seven different colon carcinoma cell lines. By sequencing, we analysed mutational status of Chibby. To test whether Chibby exhibited effects onβ-catenin signalling in colon carcinoma cells, we transfected SW480 cells with Chibby expression plasmid and, subsequently, analysed activity of p-catenin and tested for alterations in cellular phenotype. In addition, we examined Chibby mRNA levels in samples of colorectal carcinomas and adjacent normal tissues by using quantitative RT-PCR and hybridised gene chips with samples from CRC and normal tissues. RESULTS: Chibby mRNA expression was strongly down-regulated in colon carcinoma cell lines in comparison to normal colon epithelial cells and no mutation in any of the examined colon carcinoma cell lines was found. Further, we could show that Chibby inhibited p-catenin activity in TOPflash assays when over-expressed in SW480 cells. Proliferation and invasion assays with Chibby transfected SW480 cells did not reveal profound differences compared to control cells. In contrast to these in vitro data, quantitative RT-PCR analyses of Chibby mRNA levels in CRC tumor samples did not show significant differences to specimens in adjacent non-cancerous tissue. Consistent with these findings, gene chips analysing tissue samples of tumors and corresponding normal tissue did not show altered Chibby expression CONCLUSION: Altered Chibby expression might be observed in vitro in different colon carcinoma cell lines. However, this finding could not be confirmed in vitro in CRC tumors, indicating that Chibby is not likely to promote CRC tumor development or progression. As Chibby is an important inhibitor ofβ-catenin signalling, our data implicate that the usability of colon carcinoma cell lines for in vitro studies analysing the Wnt/β-catenin pathway in colorectal carcinoma needs extensive verification. | Marion M Schuierer Elisabeth Graf Ken-Ichi Takemaru Wolfgang Dietmaier Anja-Katrin Bosserhoff | 2006 | World Journal of Gastroenterology2006，12，10： | 5 |
| 2 | Minimally invasive surgery for resection of duodenal carcinoid tumors: endoscopic full-thickness resection under laparoscopic observation显示文摘 | Hironori Tsujimoto Takashi Ichikura Shigeaki Nagao Tomoki Sato Satoshi Ono Satoshi Aiko Shuichi Hiraki Yoshihisa Yaguchi Naoko Sakamoto Takemaru Tanimizu Junji Yamamoto Kazuo Hase | 2010 | Surgical Endoscopy2010，，2： | 2 |
| 3 | Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation显示文摘 | TAKEMARU K I HARASHIM S UEDA H | 1998 | Molecular Cell Biology1998，18，9： | 1 |
| 4 | A nuclear β-catenin-associated antagonist of the Wnt/wingless pathway显示文摘 | Takemaru KI Yamaguchi S Lee YS | 2003 | Nature2003，422，： | 1 |
| 5 | Isolation and genetic of auxotrophic mutants in Flmmulina velutipes显示文摘 | TAKEMARU T SUZUKI M MIGSKI N | 1995 | Trans Mycol Soc Japan1995，36，： | 1 |
| 6 | Chibby,a nuclear beta-catenin-associated antagonist of the Wnt/Wing-less pathway显示文摘 | Takemaru K Yamaguchi S Lee YS | 2003 | Nature2003，422，6934： | 1 |
| 7 | The transcriptional coactivator CBP interacts with beta-catenin to activate gene expression显示文摘 | Takemaru K I Moon R T | 2000 | Cell Bio2000，，149： | 1 |
| 8 | Multiprotein bridg- ing factor 1 (MBF1) is an evolutionarily conserved transcrip- tional coactivator that connects a regulatory factor and TATA element-binding protein显示文摘 | TAKEMARU K LI F Q UEDA H | 1997 | Proceedings of the National Acade- my of Sciences of the United States of America1997，94，14： | 1 |
| 9 | Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation显示文摘 | TAKEMARU K HARASHIMA S UEDA H | 1998 | Molecular and Cellular Biology1998，18，9： | 1 |
| 10 | Chibby,a nuclearβ-catenin-associated antagonist of the Wnt/Wingless pathway显示文摘 | TAKEMARU K YAMAGUCHI S LEE Y S | 2003 | Nature2003，422，6934： | 1 |
| 11 | The transcriptional coactivator CBP interacts with beta-catenin to activate gene expression 显示文摘 | Takemaru KI Moon RT | 2000 | J Cell Biol2000，149，2： | 1 |
| 12 | The transcriptional coactivator CBP interacts with beta-catenin to activate gene expression显示文摘 | Takemaru K I Moon R T | 2000 | J Cell Biol2000，149，2： | 1 |
| 13 | Lymphoid enhancer factor-1 links two hereditary leukemia syndromes through core-binding factor alpha regulation of ELA2显示文摘 | LI F Q PERSON R E TAKEMARU K | | 0，，04： | 1 |
| 14 | Muhiprotein bridg- ing factor 1 (MBFI) is an evolutionarily conserved transcrip- tional coactivator that connects regulatory factor and TATA el- ement-binding protein 显示文摘 | TAKEMARU K LI F Q UEDA H | 1997 | Proc Natl Acad Sci1997，94，： | 1 |
| 15 | Yeast coactivator MBFI mediates GCN4-dependent transcriptional ac- tivation 显示文摘 | TAKEMARU K HARASHIMA S UEDA H | 1998 | Mol Cell Bio1998，18，： | 1 |
| 16 | Identification of the core domain and the secondary structure of the tran- scriptional coactivator MBFI显示文摘 | OZAKI J TAKEMARU K IKEGAMI T | 1999 | Genes Cells1999，4，： | 1 |
| 17 | The transcriptional coactivator CBP interacts with beta-catenin to activate gcne expression 显示文摘 | Takemaru K I Moon R T | 2000 | J Cell Biol2000，149，2： | 1 |
| 18 | Effect of josamyein administration on intestinal mieroflra of cultured yellow tail显示文摘 | TAKEMARU I FUSUDA R | 1988 | Nippon Suisan Gakkaishi1988，54，50： | 1 |
| 19 | The transcriptional coac- tivator CBP interacts with beta-catenin to activate gene expression显示文摘 | Takemaru KI Moon RT | 2000 | J Cell Biol2000，149，2： | 1 |
| 20 | Dapper, a Dishevelled-Associated Antagonist of β-Catenin and JNK Signaling, Is Required for Notochord Formation显示文摘 | Benjamin N.R. Cheyette Joshua S. Waxman Jeffrey R. Miller Ken-Ichi Takemaru Laird C. Sheldahl Natasha Khlebtsova Eric P. Fox Thomas Earnest Randall T. Moon | 2002 | Developmental Cell2002，，4： | 1 |
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