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| 1 | Pea3 expression promotes the invasive and metastatic potential of colorectal carcinoma显示文摘AIM:To investigate the function of Pea3 in colorectal carcinoma(CRC)invasion and metastatic potential.METHODS:The expression of Pea3 during clinical progression of human CRC was investigated using Oncomine Research Edition.To assay Pea3 expression in established CRC cell lines,we performed western blotting of cell lysates.We employed sh RNA-mediated knockdown of Pea3 in HCT116(HCT)and LS174T CRC cells which was confirmed by real-time quantitative PCR(q PCR)and western blotting.Transwell invasion assays,MTS proliferation assays,anoikis assays,and fluorometric matrix metalloprotease(MMP)assays wereperformed to determine the effects of Pea3 knockdown on invasion,proliferation,anoikis and MMP activity in CRC cells in vitro.Alterations in epithelial-mesenchymal transition(EMT)and matrix metalloprotease(MMP)m RNA levels were determined by q PCR.CRC cells were injected into the flanks of nude mice to generate xenografts and tumor growth monitored with serial calliper measurements.To assay metastatic potential,CRC cells were injected into the spleen of nude mice,and histological analysis performed on the livers 21 d later.RESULTS:We demonstrated that reduction of Pea3expression in CRC cells significantly impaired their invasive capacity(HCT.sh Pea3,0.28±0.04 fold,P<0.01;LS.sh Pea3,0.15±0.04 fold;SW.sh Pea3,0.23±0.03,P<0.01),reduced anoikis resistance(HCT.sh Pea3 75.4%±1.9%viable cells vs HCT.sh Ctrl 88.6%±0.6%viable cells,P<0.01;LS.sh Pea3 71.7%±0.5%viable cells vs LS.Ctrl 89.6%±0.3%viable cells,P<0.005,but had no effect on proliferation(HCT.sh Ctrl AUC 5098±123 vs HCT.sh Pea3 5689±151,P<0.05;LS.sh Ctrl AUC 5600±324.1 vs LS.sh Pea3 6423±400,P<0.05).In vivo,HCT.sh Pea3 and HCT.sh Ctrl tumour xenografts grew at a similar rate(HCT.sh Pea3 2.64±0.82 fold vs HCT.sh Ctrl 2.88±0.80 fold,P>0.05).In keeping with a pro-metastatic function for Pea3 in CRC,several EMT markers and MMPs were downregulated in sh Pea3-expressing cells,suggesting that Pea3 may exert its effects through these processes.A reduction in overall MMP activity was observed in HCT.sh Pea3 cells compared to their control counterparts(HCT.sh Pea30.61±0.04 fold,P<0.005).This translated in vivo to the complete absence of metastases in the livers of mice that were grafted with CRC cells lacking Pea3.Conversely,CRC cells expressing Pea3 formed liver metastases in all mice.CONCLUSION:Our study implicates Pea3 as a mediator of metastases,and provides a biological rationale for the adverse prognosis associated with elevated Pea3 expression in human CRC. | Aruz Mesci Samira Taeb Xiaoyong Huang Rishi Jairath Darshan Sivaloganathan Stanley K Liu | 2014 | World Journal of Gastroenterology2014，20，46： | 7 |
| 2 | CoolClot,a novel hemostatic agent for controlling life-threatening arterial bleeding显示文摘BACKGROUND:Uncontrolled bleeding is the first leading cause of preventable death in the battlefield and the 2nd cause of mortality in civil accidents.Incompressible hemorrhage control is among the interventions that drastically increase the survival rate in wound individuals.We have previously shown that a certain mixture of bentonite and zeolite minerals can significantly decrease the bleeding in rats.METHODS:In this study,nine healthy hybrid dogs were selected and after induction of anesthesia with ether,either arterial puncture by a needle or arteriotomy was performed on both groin regions of the dogs.For control arteries(either the right or left femoral artery),only pressure by sterilized gauze was performed,while for the femoral arteries of the opposite side,our invented hemostatic agent,namely CoolClot,was topically used before applying the pressure.In the second stage of the study,to assess the coagulation time,blood samples were collected from 10 volunteer students.RESULTS:CoolClot significantly decreased the bleeding time in animals whose femoral arteries were cut or punctured.In the human phase of the study,the mean coagulation time in control blood samples was 253.4+44.1 seconds,whereas it was 149.5+50.0,162.3±74.6 and 143.4±114.6seconds,respectively in blood samples treated with bentonite,zeolite and CoolClot(P<0.05).CONCLUSIONS:As controlling bleeding after a life-threatening arterial damage is critical for increasing the chance of survival,the results obtained in this study indicate the significant efficacy of CoolClot in shortening the bleeding time.Our experiments also indicate that CoolClot can significantly reduce the clotting time in human blood samples. | SMJ Mortazavi A Tavasoli M Atefi N Tanide N Radpey P Roshan-shomal H Moradi S Taeb | 2013 | World Journal of Emergency Medicine2013，4，2： | 2 |
| 3 | Association between genes controlling flowering time and shoot sodium accumulation in the triticeae显示文摘 | Taeb M Koebner R M D Forster B P | 1992 | Plant Soil1992，146，： | 1 |
| 4 | Utilization of Copper Slag in the Cement Industry显示文摘 | Taeb A Faghihi S | 2002 | ZKG International2002，55，4： | 1 |
| 5 | Spontaneous hemopneumothorax显示文摘 | Taebe S Kanazawa H Yamazaki Y et a1 | 1996 | Ann Rhorae Surg1996，62，7： | 1 |
| 6 | GQ1b ganglioside antibody-related disorders:a case with a complex phenotype显示文摘 | Pers YM Taeb G Ayrignac X | | 0，，04： | 1 |
| 7 | Study of correlations and relations between plant yieldand quantitative and qualitative other traits in safflower显示文摘 | Bagheri A Yazdi S B Taeb M | | Iranian Journal of Agricultural Sciences0，32，2： | 1 |
| 8 | 显示文摘 | Moradi G R Basir M M Taeb A | 2003 | Catalysis Communications2003，4，： | 1 |
| 9 | Expression of prostate stem cell antigen (PSCA) in prostate cancer: a tissue microarray study of Iranian patients 显示文摘 | Taeb J Asgari M Abolhasani M | 2014 | Pathology Research and Practice2014，210，1： | 1 |
| 10 | Synthesis, characterization and electrochemical study of synthesis of a new Schiff base (H 2 cddi t butsalen) ligand and their two asymmetric Schiff base complexes of Ni(II) and Cu(II) with NN′OS coordination spheres显示文摘 | Saeid Menati Azadeh Azadbakht Abbas Taeb Ali Kakanejadifard Hamid Reza Khavasi | 2012 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy2012，，： | 1 |
| 11 | The effect photoperiod insensitivity on salt tolerance of amphiploids between bread wheat (Triticum aestivum) and sand couch grass ( Thinopyrum bessarabicum) 显示文摘 | Martin P K Taeb M Koebner R M | 1993 | Plant Breeding1993，111，： | 1 |
| 12 | Study of correlations andrelations between plant yield and quantitative and qualitative other traits in saf- flower显示文摘 | Bagheri A Yazdi sb Taeb M | 2001 | Iranian Journal of Agricultural Sciences2001，32，2： | 1 |
| 13 | Environmental sustainability and water availability: Analyses of the scarcity and improvement oppor- tunities in the Usangu plain, Tanzania显示文摘 | Malley Z J U Taeb M Matsumoto T | 2009 | Physics and Chemistry of the Earth2009，34，： | 1 |
| 14 | Promotion of CO/SiO2 Fischer-Tropsch catalysts with zirconium显示文摘 | Moradi G R Basir M M Taeb A | 2003 | Catalysis Communications2003，4，1： | 1 |
| 15 | First flush pollution load of urban stormwater runoff显示文摘 | TAEB I A DROSTE R L | 2004 | Journal of Environmental Engineering2004，3，： | 1 |
| 16 | miRNA-95 mediates radioresistance in tumors by targeting the sphingalipid phosphatase SGPP1 显示文摘 | Huang X Taeb S Jahangiri S | 2013 | Cancer Res2013，73，23： | 1 |
| 17 | Promotion of Co/SiO2 Fischer-Tropsch catalysts with zirconium显示文摘 | MORADI G R BASIR M M TAEB A KIENNEMANN A | 2003 | Catal Commun2003，4，1： | 1 |
| 18 | Promotion of Co/SiC>2Fischer-Tropsch catalysts with zirconium显示文摘 | Moradi G R Basir M M Taeb A | 2003 | CatalysisCommunications2003，4，： | 1 |
| 19 | Spontaneous hemopneumothorax显示文摘 | Taebe S Kanazawa H Yamazaki Y | 1996 | Ann Thorac Surg1996，62，7： | 1 |
| 20 | Highly selective Me-ZSM-5catalyst for methanol to propylene(MTP)显示文摘 | Rostamizadeh M Taeb A | 2015 | J Ind Eng Chem2015，27，： | 1 |
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