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| 1 | Persistent occult hepatitis B virus infection:Experimental findings and clinical implications显示文摘Hepatitis B virus (HBV) is a highly pathogenic virus that causes chronic liver diseases in millions of people globally. In addition to a symptomatic, serologically evident infection, occult persistent HBV carriage has been identified since nucleic acid amplification assays of enhanced sensitivity became introduced for detection of hepadnaviral genomes and their replicative intermediates. Current evidence indicates that occult HBV infection is a common and long-term consequence of resolution of acute hepatitis B. This form of residual infection is termed as secondary occult infection (SOI). The data from the woodchuck model of HBV infection indicate that exposure to small amounts of hepadnavirus can also cause primary occult infection (POI) where virus genome, but no serological makers of exposure to virus, are detectable, and the liver may not be involved. However, virus replicates at low levels in the lymphatic system in both these forms. We briefly summarize the current understanding of the nature and characteristics of occult hepadnaviral persistence as well as of its documented and expected pathological consequences. | Patricia M Mulrooney-Cousins Tomasz I Michalak | 2007 | World Journal of Gastroenterology2007，13，43： | 17 |
| 2 | Underexpression of LATS1 TSG in colorectal cancer is associated with promoter hypermethylation显示文摘AIM:To investigate large tumor suppressor 1 (LATS1 ) expression, promoter hypermethylation, and microsatellite instability in colorectal cancer (CRC).METHODS:RNA was isolated from tumor tissue of 142 CRC patients and 40 colon mucosal biopsies of healthy controls. After reverse transcription, quantitative polymerase chain reaction (PCR) was performed, and LATS1 expression was normalized to expression of the ACTB and RPL32 housekeeping genes. To analyze hypermethylation, genomic DNA was isolated from 44 tumor CRC biopsies, and methylation-specific PCR was performed. Microsatellite instability (MSI) status was checked with PCR using BAT26, BAT25, and BAT40 markers in the genomic DNA of 84 CRC patients, followed by denaturing gel electrophoresis. RESULTS:Decreased LATS1 expression was found in 127/142 (89.4%) CRC cases with the average ratio of the LATS1 level 10.33 ± 32.64 in CRC patients vs 32.85 ± 33.56 in healthy controls. The lowest expression was found in Dukes' B stage tumors and G1 (welldifferentiated) cells. Hypermethylation of the LATS1 promoter was present in 25/44 (57%) CRC cases analyzed. LATS1 promoter hypermethylation was strongly associated with decreased gene expression; methylated cases showed 162× lower expression of LATS1 than unmethylated cases. Although high-grade MSI (mutation in all three markers) was found in 14/84 (17%) cases and low-grade MSI (mutation in 1-2 markers) was found in 30/84 (36%) cases, we found no association with LATS1 expression. CONCLUSION:Decreased expression of LATS1 in CRC was associated with promoter hypermethylation, but not MSI status. Such reduced expression may promote progression of CRC. | Piotr M Wierzbicki Krystian Adrych Dorota Kartanowicz Marcin Stanislawowski Anna Kowalczyk Janusz Godlewski Iwona Skwierz-Bogdanska Krzysztof Celinski Tomasz Gach Jan Kulig Bartlomiej Korybalski Zbigniew Kmiec | 2013 | World Journal of Gastroenterology2013，19，27： | 8 |
| 3 | Three-dimensional perfused human in vitro model of nonalcoholic fatty liver disease显示文摘AIM To develop a human in vitro model of non-alcoholic fatty liver disease(NAFLD), utilising primary hepatocytes cultured in a three-dimensional(3D) perfused platform. METHODS Fat and lean culture media were developed to directly investigate the effects of fat loading on primary hepatocytes cultured in a 3D perfused culture system. Oil Red O staining was used to measure fat loading in the hepatocytes and the consumption of free fatty acids(FFA) from culture medium was monitored. Hepatic functions, gene expression profiles and adipokine release were compared for cells cultured in fat and lean conditions. To determine if fat loading in the system could be modulated hepatocytes were treated with known anti-steatotic compounds. RESULTS Hepatocytes cultured in fat medium were found to accumulate three times more fat than lean cells and fat uptake was continuous over a 14-d culture. Fat loading of hepatocytes did not cause any hepatotoxicity and significantly increased albumin production. Numerous adipokines were expressed by fatty cells and genes associated with NAFLD and liver disease were upregulated including: Insulin-like growth factorbinding protein 1, fatty acid-binding protein 3 and CYP7A1. The metabolic activity of hepatocytes cultured in fatty conditions was found to be impaired and the activities of CYP3A4 and CYP2C9 were significantlyreduced, similar to observations made in NAFLD patients. The utility of the model for drug screening was demonstrated by measuring the effects of known antisteatotic compounds. Hepatocytes, cultured under fatty conditions and treated with metformin, had a reduced cellular fat content compared to untreated controls and consumed less FFA from cell culture medium.CONCLUSION The 3D in vitro NAFLD model recapitulates many features of clinical NAFLD and is an ideal tool for analysing the efficacy of anti-steatotic compounds. | Tomasz Kostrzewski Terri Cornforth Sophie A Snow Larissa Ouro-Gnao Cliff Rowe Emma M Large David J Hughes | 2017 | World Journal of Gastroenterology2017，23，2： | 6 |
| 4 | Simultaneous determination of N-oxides and free bases of pyrrolizidine alkaloids by cation-exchange solid-phase and ion-pair high-performance liquid chromatography显示文摘 | TOMASZ M KAZIMIERZ G ANNA W | 2002 | J Chromatography A2002，949，12： | 1 |
| 5 | Effects of plyometric training on maximal power output and jumping ability显示文摘 | HUBERT M TOMASZ S | | 0，，01： | 1 |
| 6 | Cu/Cr203'3A1203 and Au-Cu/Cr203 '3A1203 catalysts for methanol synthesis and water gas shift reactions显示文摘 | Pawel M Tomasz M Waldemar M | 2011 | React Kinet Mech Catal2011，104，： | 1 |
| 7 | Investigation of ash deposit formation during co-firing of coal with sewage sludge, saw-dust and refuse derived fuel显示文摘 | TOMASZ K MARCO M MICHAEL I | 2008 | Fuel2008，87，12： | 1 |
| 8 | On-line strcture characterization of pyrrolizidine alkaloids in Onosma stel- lulatum and Emilia coccinea by liquid chromatography-ion-trap mass spectrometry显示文摘 | TOMASZ M KARINE N KAZIMIERZ G | 2004 | J Chromatography A2004，1056，12： | 1 |
| 9 | Solubility of pyromorphite Pb5 (P04 )3 Cl-mimetite Pb5 ( As04 )3 Cl solid solution series显示文摘 | Justyna F Maciej M Tomasz B | 2011 | Geochimica et Cosmochimica Acta2011，75，7： | 1 |
| 10 | Robust method to retrieve the constitutive effective parameters of metamaterials 显示文摘 | Chen Xudong Grzegorczyk Tomasz M Wu Baeian | 2004 | Physical Review E2004，70，01： | 1 |
| 11 | Short-dayand melatonin effects on milking parameters, prolactin profilesand growth-hormone secretion in lactating sheep 显示文摘 | EDYTA M TOMASZ M KATARZYNA R | 2013 | SmallRuminant Research*2013，109，： | 1 |
| 12 | Short-day and me- latonin effects on milking parameters, prolactin profiles and growth-hormone secretion in lactating sheep显示文摘 | Edyta M Tomasz M Katatxyna R | 2013 | Small Rumi- nant Research2013，109，： | 1 |
| 13 | Prevalence of genetic risk factors related with thrombophilia and hypofibrinolysis in patients with osteonecrosis of the femoral head in Poland显示文摘 | Jacek G Monika B Tomasz M | 2013 | BMC Muscu- loskeletal Disorders2013，14，1： | 1 |
| 14 | Enzymology of mitomycin C metabolic activation in tumour tissue: implications for enzyme-directed bioreductive drug development 显示文摘 | Cummings J Spanswick VJ Tomasz M | 1998 | Biochem Pharmacol1998，56，4： | 1 |
| 15 | Asymmetric, stereocontrolled total synthesis of ( - )-B-re- vianamide B 显示文摘 | Robert M Williams Tomasz Glinka Ewa Kwas | 1990 | J Am Chem Soc1990，112，： | 1 |
| 16 | Transistor paint:high mobilities in small bandgap polymer semiconductor based on the strong acceptor,diketopyrrolopyrrole and strong donor,dithienopyrrole显示文摘 | Toby L N Tomasz M Y Jun Y L Mishra S P Belot J A Balliet C L Javier A E Kowalewski T McCullough R D | 2010 | Advanced Materials2010，22，41： | 1 |
| 17 | Antithrombotic effect of captopril and losartan is mediated by angiotensin-(1-7)显示文摘 | Iwona K Robert P Tomasz M | 2002 | Hypertension2002，40，5： | 1 |
| 18 | Acute and late obstruction of a modified Blalock - Taussig shunt: a two-center experience in different catheter-based methods of treatment 显示文摘 | Tomasz M Maria Z Krzysztof WM | 2010 | Interact Cardio- Vasc Thorac Surg2010，10，1： | 1 |
| 19 | Robust method to retrieve the constitu- tive effective parameters of metamaterials 显示文摘 | CHEN Xu-dong GRZEGORCZYK Tomasz M WU Bae Jan | 2004 | Plays Rev E2004，70，01： | 1 |
| 20 | Enzymology of mitomycin C metabolic activation in tumour tissue: implications for enzyme-directed bioreductive drug development显示文摘 | Cummings J Spanswick VJ Tomasz M | 1998 | Biochem Pharmacol1998，56，4： | 1 |
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