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| 1 | Establishment and evaluation of a peanut association panel and analysis of key nutritional traits显示文摘Breeding programs aim to improve the yield and quality of peanut(Arachis hypogaea L.); using association mapping to identify genetic markers linked to these quantitative traits could facilitate selection efficiency. A peanut association panel was established consisting of 268 lines with extensive phenotypic and genetic variation, meeting the requirements for association analysis. These lines were grown over 3 years and the key agronomic traits, including protein and oil content were examined. Population structure(Q) analysis showed two subpopulations and clustering analysis was consistent with Q-based membership assignment and closely related to botanical type. Relative Kinship(K) indicated that most of the panel members have no or weak familial relatedness, with 52.78% of lines showing K ? 0. Linkagedisequilibrium(LD) analysis showed a high level of LD occurs in the panel. Model comparisons indicated false positives can be effectively controlled by taking Q and K into consideration and more false positives were generated by K than Q. A preliminary association analysis using a Q t K model found markers significantly associated with oil, protein, oleic acid, and linoleic acid, and identified a set of alleles with positive and negative effects. These results show that this panel is suitable for association analysis,providing a resource for marker-assisted selection for peanut improvement. | Xiurong Zhang Suqing Zhu Kun Zhang Yongshan Wan Fengzhen Liu Qingfang Sun Yingjie Li | 2018 | Journal of Integrative Plant Biology2018，60，3： | 5 |
| 2 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 3 | Effect of Soil Drying Intensity During an Experimental Drying-Rewetting Event on Nutrient Transformation and Microbial Community Composition显示文摘Soil drying-rewetting(DRW) events affect nutrient transformation and microbial community composition; however, little is known about the influence of drying intensity during the DRW events. Therefore, we analyzed soil nutrient composition and microbial communities with exposure to various drying intensities during an experimental drying-rewetting event, using a silt loam from a grassland of northern China, where the semi-arid climate exposes soils to a wide range of moisture conditions, and grasslands account for over 40% of the nation's land area. We also conducted a sterilization experiment to examine the contribution of soil microbes to nutrient pulses. Soil drying-rewetting decreased carbon(C) mineralization by 9%–27%. Both monosaccharide and mineral nitrogen(N) contents increased with higher drying intensities(drying to ≤ 10% gravimetric water content), with the increases being 204% and 110% with the highest drying intensity(drying to 2% gravimetric water content), respectively, whereas labile phosphorus(P)only increased(by 105%) with the highest drying intensity. Moreover, levels of microbial biomass C and N and dissolved organic N decreased with increasing drying intensity and were correlated with increases in dissolved organic C and mineral N, respectively,whereas the increases in labile P were not consistent with reductions in microbial biomass P. The sterilization experiment results indicated that microbes were primarily responsible for the C and N pulses, whereas non-microbial factors were the main contributors to the labile P pulses. Phospholipid fatty acid analysis indicated that soil microbes were highly resistant to drying-rewetting events and that drought-resistant groups were probably responsible for nutrient transformation. Therefore, the present study demonstrated that moderate soil drying during drying-rewetting events could improve the mineralization of N, but not P, and that different mechanisms were responsible for the C, N, and P pulses observed during drying-rewetting events. | SUN Dashend BI Qingfang LI Kejie ZHU Jun ZHANG Qichun JIN Chongwei LU Lingli LIN Xianyong | 2018 | Pedosphere2018，28，4： | 2 |
| 4 | Amultimedia fate model to evaluate the fate of PAHs inSonghua River,China 显示文摘 | WANG Ce FENG Yujie SUN Qingfang | 2012 | Environmental Pollution2012，164，： | 1 |
| 5 | Polycyclic aromatic hydrocarbons in surface water fromthe Songhua River of China 显示文摘 | FENG Yujie SUN Qingfang GAO Peng | 2009 | FreseniusEnvironmental Bulletin2009，18，12： | 1 |
| 6 | Gender and magnetic resonance imaging classification-related differences in clinical and biochemical characteristics of Cushing's disease: a single-centre study显示文摘 | Chen Yufan Mei Xiaobo Jian Fangfang Ma Qinyun Chen Xiao Bian Liuguan Sun Qingfang | 2014 | Chinese Medical Journal2014，，22： | 1 |
| 7 | A multimedia fate model to evaluate the fate of PAHs in Songhua River,China显示文摘 | Wang Ce Feng Yujie Sun Qingfang | 2012 | Environ Pollut2012，164，： | 1 |
| 8 | Polycyclic aromatic hydrocarbons in surface water from the Songhua River of China 显示文摘 | FENG Yujie SUN Qingfang GAO Peng | 2009 | Fresenius Environmental Bulletin2009，18，12： | 1 |
| 9 | Bilateral inferior petrosal sinus sampling for the treatment of Cushing's disease Data from 52 cases from one institute over an eight-year period显示文摘The present study analyzed data from 108 Cushing's disease patients,who underwent trans-sphenoidal surgery in one hospital between January 2003 and April 2010,to investigate the performance value of bilateral inferior petrosal sinus sampling (BIPSS).Of the 108 patients,52 underwent BIPSS prior to surgery and 56 did not.Results showed that BIPSS accuracy for the diagnosis of Cushing's disease was 86.5% (45/52),and accuracy for adenoma lateralization during surgery was 76.9% (40/52).In addition,early remission rate was significantly greater compared to patients without BIPSS.Results demonstrated that BIPSS facilitated localization and diagnosis of Cushing's disease and helped to predict adenoma lateralization. | Changyan Fan Xiuhua Shi Qingfang Sun Liuguan Bian Weiguo Zhao Jiankang Shen Hua Zhang Tingwei Su Weiqing Wang Xiaoying Li Guang Ning Liang Kong Lingling Hu | 2011 | Neural Regeneration Research2011，6，28： | 1 |
| 10 | Polymorphisms of ERCC1, XPD, XRCC1 and XPG Predict Clinical Outcome in Advanced Gastric Cancer Patients Receiving Oxaliplatin-Based Chemotherapy in Chinese Population显示文摘OBJECTIVE To investigate whether polymorphisms in ERCC1,XPD,XPG,XRCC1 genes are associated with clinical outcomes inadvanced gastric cancer (AGC) patients treated with oxaliplatin-based chemotherapy.METHODS The genetic polymorphisms in ERCC1,XPD,XPG,XRCC1 were determined in 94 advanced gastric cancer patientstreated with oxaliplatin-based chemotherapy,using TaqMan-MGBprobes.The clinical response of 60 patients with stage Ⅳ disease,time to progression (TTP) and overall survival (OS) of 94 patientswere evaluated.RESULTS The overall disease control rate (CR + PR + SD) of the60 patients in stage Ⅳ was 70% (42/60).Patients with XRCC1 399G/G,XPG 46 C/C genotypes showed enhanced response to theoxaliplatin-based chemotherapy compared to those with othergenotypes (P<0.05).The median OS and TTP of the patientswere 5.5 months and 9.0 months,respectively.Among the 4types of polymorphisms in the study,XRCC1 399 G/A + A/A,XPG 46 C/T + T/T genotypes were regarded to be associatedwith chemoresistance and poor survival (P<0.05).Combinationanalysis of the 2 polymorphisms using the Kaplan-Meier methodrevealed that the TTP and OS of the patients with a number of riskgenotypes were significantly shortened (P<0.05).No significantassociation was found between the genotypes of the XPD codon751,the ERCC1 codon 118 and the clinical outcome (P>0.05).CONCLUSION Testing for XRCC1 399,XPG 46 polymorphismsmay allow identification of the gastric cancer patients whowill benefit from oxaliplatin-based chemotherapy.Specificpolymorphisms may influence clinical outcomes of AGCpatients.Selecting specific chemotherapy based on pretreatmentgenotyping represents an innovative strategy that warrantsprospective studies. | Jian Jiang Jun Liang Ruyong Yao Qingfang Li Shanai Song Yingying Sun | 2009 | Clinical oncology and cancer resexreh2009，6，5： | 1 |
| 11 | A multimedia fate model to evaluate the fate of PAHs in Songhua River, China显示文摘 | WANG Ce FENG Yujie SUN Qingfang | 2012 | Environment Pollution2012，164，： | 1 |
| 12 | A multimedia fate model to evaluate the fate of PAHs in Songhua River, China显示文摘 | Ce Wang Yujie Feng Qingfang Sun Shanshan Zhao Peng Gao Bai-Lian Li | 2012 | Environmental Pollution2012，，： | 1 |
| 13 | Variation of Electromagnetic Tornogram Before and After Earthquakes显示文摘Cross-well electromagnetic tomography has been used to monitor the changes of the abso rption coefficient of electromagnetic waves before and after earthquakes for 5 years at Dahuichang fault since 1992.It has been found that 3 months before the occurrence of moderately strong earthquakes(ML≥4.0,△<210 km),several special changes in tomogram appeared as follows:The mean-value of the absorption coefficient dropped about 10% compared with the annually decreasing tendency; the mean difference of pixels suddenly decreased or increased in different directions; and the entropy of the paragenic matrix in a tomogram decreased and the number of low absorption pixels increased.It is inferred that such changes in electromagnetic tomograms are attributed to the variation of micro-cracks and porosity in rocks.Electromagnetic tomography can be studied as a new technique to detect earthquake precursors. | Sun Cichang,Wang Xusheng,Liu Qingfang,Tao Yulu,Yu Ping,and Feng RuiInstitute of Geophysics,SSB,Beijing 100081,China Department of Geophysics,Peking University,Beijing 100871,China Seismnlogical Bureau of Hunan Province,Changsha 410001,China Center for Seismological Data and Information,SSB,Beijing 100045,China | 1998 | Earthquake Research in China1998，12，2： | 0 |
| 14 | Identification and analysis of a minority fraction in a U87 cell line Do side-population cells represent bona fide stem cell-like cancer cells?显示文摘BACKGROUND:Overwhelming evidence suggests that tumor bulks are comprised of differentiated tumor cells and cancer stem cells(CSCs).The stem cell-like side-population(SP) cells account for a minor fraction of the total tumor cells,yet are apparently the cells capable of tumor initiation,growth, maintenance,and recurrence. OBJECTIVE:To identify potential stem cell-like cancer cells in a U87 human brain glioma cell line on the basis of dye efflux,clone formation,and multi-drug resistance capacity. DESIGN,TIME AND SETTING:The cellular and molecular biology experiment was performed at the Laboratory of Shanghai Institute of Hematology and Laboratory of Shanghai Institute of Endocrinology in Ruijin Hospital;in vivo contrast observational animal trial was performed at Experimental Animal Center,School of Medicine,Shanghai Jiao Tong University from June 2007 to May 2008. MATERIALS:The U87 cell line was provided by the Shanghai Institute of Cancer Research, Chinese Academy of Science;DMEM/F12(1:1) and fetal bovine serum were purchased from Gibco, Invitrogen,USA;human recombinant basic fibroblast growth factors were purchased from BD Bioscience,USA;Hoechst 33342,Verapamil,and methyl thiazolyl tetrazolium were purchased from Sigma,USA;phycoerythrin-labeled anti-human-CD133 was purchased from Milteny Biotec, Germany;SYBR(?) PrimeScript^(TM) RT-PCR kit was purchased from TaKaRa Biotechnology,Dalian, China. METHODS:Monolayer cultured cells were harvested by 0.25%Trypsin-EDTA and suspended at a 1×10~6/mL dilution in PBS containing 2%FBS,and were stained with Hoechst 33342 dye,either alone or in combination with Verapamil.Following fluorescence-activated cell sorting,SP and non-SP subsets were cultivated with serum-containing(DMEM plus 10%fetal bovine serum) or serum-free culture medium[DMEM/F12(1:1) + 1×B27 supplement + 10 ng/mL basic fibroblast growth factors + 1×L-glutamine]to determine growth characteristics in vitro.Finally,single free U87 cells and subsets(SP or non-SP cells) were subcutaneously injected into the backs of 5-week-old nude mice for in vivo tumorigenicity. MAIN OUTCOME MEASURES:Cell morphology and clonogenicity were observed under inverted microscope;SP phenotype and fluorescent antibody labeling were analyzed by MoFlo^(TM) flow cytometry;ABC transporter mRNA expression was evaluated by semi-quantitative real-time RT-PCR;efflux capacity for anti-neoplastic drugs from the U87 cell line and subsets was measured with the MTT assay,then detected by enzyme-linked immunosorbent assay at a wavelength of 490 nm;in vivo tumorigenicity in immunodeficient nude mice was evaluated by diameter size. RESULTS:During in vitro passages,human U87 cells maintained a stable SP fraction profile and exhibited the ability to form neurosphere-like clones.SP cell proliferation decreased compared with non-treated U87 cells.CD133 expression was reduced in the SP and non-SP cells.Freshly sorted SP fractions expressed higher levels of ABC drug transporter genes,and exhibited increased potential for cytotoxic drug resistance.The in vivo malignancy of U87 cells was largely dependent on non-SP cells in nude mice,and tumors that formed from the non-SP fraction developed faster and larger compared with tumors from the SP fraction. CONCLUSION:The SP cell component was a key factor that influenced mRNA expression and cytotoxic drug resistance.In particular,cancer stem cells or tumor-initiating cells were not exclusively enriched in the SP subset of the U87 cell line,and non-SP cells were even more tumorigenic. | Changwei Gu Qingfang Sun Jiang Zhu Liuguan Bian Weiguo Zhao Jiankang Shen HengGao Dongxia Feng | 2009 | Neural Regeneration Research2009，4，11： | 0 |
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