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| 1 | Genome-Wide Linkage and Positional Association Analyses Identify Associations of Novel AFF3 and NTM Genes with Triglycerides:The GenSalt Study显示文摘We conducted a genome-wide linkage scan and positional association study to identify genes and variants influencing blood lipid levels among participants of the Genetic Epidemiology Network of Salt-Sensitivity(Gen Salt) study. The Gen Salt study was conducted among1906 participants from 633 Han Chinese families. Lipids were measured from overnight fasting blood samples using standard methods.Multipoint quantitative trait genome-wide linkage scans were performed on the high-density lipoprotein, low-density lipoprotein, and logtransformed triglyceride phenotypes. Using dense panels of single nucleotide polymorphisms(SNPs), single-marker and gene-based association analyses were conducted to follow-up on promising linkage signals. Additive associations between each SNP and lipid phenotypes were tested using mixed linear regression models. Gene-based analyses were performed by combining P-values from singlemarker analyses within each gene using the truncated product method(TPM). Significant associations were assessed for replication among777 Asian participants of the Multi-ethnic Study of Atherosclerosis(MESA). Bonferroni correction was used to adjust for multiple testing.In the Gen Salt study, suggestive linkage signals were identified at 2p11.2-2q12.1 [maximum multipoint LOD score(MML)=2.18 at2q11.2] and 11q24.3-11q25(MML=2.29 at 11q25) for the log-transformed triglyceride phenotype. Follow-up analyses of these two regions revealed gene-based associations of charged multivesicular body protein 3(CHMP3), ring finger protein 103(RNF103),AF4/FMR2 family, member 3(AFF3), and neurotrimin(NTM) with triglycerides(P=4 10 4, 1.00 10 5, 2.00 10 5, and1.00 10 7, respectively). Both the AFF3 and NTM triglyceride associations were replicated among MESA study participants(P=1.00 10 7and 8.00 10 5, respectively). Furthermore, NTM explained the linkage signal on chromosome 11. In conclusion, we identified novel genes associated with lipid phenotypes in linkage regions on chromosomes 2 and 11. | Changwei Li Lydia A.L.Bazzano Dabeeru C.Rao James E.Hixson Jiang He Dongfeng Gu Charles C.Gu Lawrence C.Shimmin Cashell E.Jaquish Karen Schwander De-Pei Liu Jianfeng Huang Fanghong Lu Jie Cao Shen Chong Xiangfeng Lu Tanika N.Kelly | 2015 | Journal of Genetics and Genomics2015，42，3： | 1 |
| 2 | 内皮系统基因与血压改变和高血压发病的关系:盐敏感遗传流行病学研究显示文摘研究者在一个纵向家庭研究中以单标记和新基因为基础的方法探讨内皮系统基因与血压改变和高血压发病率的关系。方法:盐敏感性的遗传流行病学随访研究共纳入633个汉族家庭的1768位成员。使用随机零点血压计,获取基线和随访调查的共9次血压值。 | Fangchao Liu Jiang He Dongfeng Gu Dabeeru C.Rao Jianfeng Huang James E.Hixson Cashell E.Jaquish Jichun Chen Changwei Li Xueli Yang Jianxin Li Treva K.Rice Lawrence C.Shimmin Tanika N.Kelly | 2015 | 中华高血压杂志2015，23，10： | 0 |
| 3 | Identification and analysis of a minority fraction in a U87 cell line Do side-population cells represent bona fide stem cell-like cancer cells?显示文摘BACKGROUND:Overwhelming evidence suggests that tumor bulks are comprised of differentiated tumor cells and cancer stem cells(CSCs).The stem cell-like side-population(SP) cells account for a minor fraction of the total tumor cells,yet are apparently the cells capable of tumor initiation,growth, maintenance,and recurrence. OBJECTIVE:To identify potential stem cell-like cancer cells in a U87 human brain glioma cell line on the basis of dye efflux,clone formation,and multi-drug resistance capacity. DESIGN,TIME AND SETTING:The cellular and molecular biology experiment was performed at the Laboratory of Shanghai Institute of Hematology and Laboratory of Shanghai Institute of Endocrinology in Ruijin Hospital;in vivo contrast observational animal trial was performed at Experimental Animal Center,School of Medicine,Shanghai Jiao Tong University from June 2007 to May 2008. MATERIALS:The U87 cell line was provided by the Shanghai Institute of Cancer Research, Chinese Academy of Science;DMEM/F12(1:1) and fetal bovine serum were purchased from Gibco, Invitrogen,USA;human recombinant basic fibroblast growth factors were purchased from BD Bioscience,USA;Hoechst 33342,Verapamil,and methyl thiazolyl tetrazolium were purchased from Sigma,USA;phycoerythrin-labeled anti-human-CD133 was purchased from Milteny Biotec, Germany;SYBR(?) PrimeScript^(TM) RT-PCR kit was purchased from TaKaRa Biotechnology,Dalian, China. METHODS:Monolayer cultured cells were harvested by 0.25%Trypsin-EDTA and suspended at a 1×10~6/mL dilution in PBS containing 2%FBS,and were stained with Hoechst 33342 dye,either alone or in combination with Verapamil.Following fluorescence-activated cell sorting,SP and non-SP subsets were cultivated with serum-containing(DMEM plus 10%fetal bovine serum) or serum-free culture medium[DMEM/F12(1:1) + 1×B27 supplement + 10 ng/mL basic fibroblast growth factors + 1×L-glutamine]to determine growth characteristics in vitro.Finally,single free U87 cells and subsets(SP or non-SP cells) were subcutaneously injected into the backs of 5-week-old nude mice for in vivo tumorigenicity. MAIN OUTCOME MEASURES:Cell morphology and clonogenicity were observed under inverted microscope;SP phenotype and fluorescent antibody labeling were analyzed by MoFlo^(TM) flow cytometry;ABC transporter mRNA expression was evaluated by semi-quantitative real-time RT-PCR;efflux capacity for anti-neoplastic drugs from the U87 cell line and subsets was measured with the MTT assay,then detected by enzyme-linked immunosorbent assay at a wavelength of 490 nm;in vivo tumorigenicity in immunodeficient nude mice was evaluated by diameter size. RESULTS:During in vitro passages,human U87 cells maintained a stable SP fraction profile and exhibited the ability to form neurosphere-like clones.SP cell proliferation decreased compared with non-treated U87 cells.CD133 expression was reduced in the SP and non-SP cells.Freshly sorted SP fractions expressed higher levels of ABC drug transporter genes,and exhibited increased potential for cytotoxic drug resistance.The in vivo malignancy of U87 cells was largely dependent on non-SP cells in nude mice,and tumors that formed from the non-SP fraction developed faster and larger compared with tumors from the SP fraction. CONCLUSION:The SP cell component was a key factor that influenced mRNA expression and cytotoxic drug resistance.In particular,cancer stem cells or tumor-initiating cells were not exclusively enriched in the SP subset of the U87 cell line,and non-SP cells were even more tumorigenic. | Changwei Gu Qingfang Sun Jiang Zhu Liuguan Bian Weiguo Zhao Jiankang Shen HengGao Dongxia Feng | 2009 | Neural Regeneration Research2009，4，11： | 0 |
| 4 | Arbitrary manipulations of focused higher-order Poincare beams by a Fresnel zone metasurface with alternate binary geometric and propagation phases显示文摘The manipulation of high-quality vector beams(VBs)with metasurfaces is an important topic and has potential for classical and quantum applications.In this paper,we propose a Fresnel zone(FZ)metasurface with metallic nanoslits arranged on FZs,which sets alternate binary geometric and propagation phases to cancel the incident spin component and focus the converted spin component(CSC).The rotation designs of nanoslits transform the incident polarization state on the conventional Poincare sphere to VBs on the higher-order Poincare(HOP)sphere.The two orbital angular momentum states of the CSCs were manipulated,and the focused HOP beams were generated.The experimental results demonstrate the broadband generation of arbitrarily focused HOP beams of high quality under the illumination of the red(632.8 nm),green(532 nm),and blue(473 nm)light.This work will be of significance for the applications of VBs in different areas,such as precision metrology,optical micromanipulation,and quantum information. | XIANGYU ZENG YUQIN ZHANG MANNA GU ZIJUN ZHAN RUIRUI ZHANG YU ZHANG RUI SUN CHANGWEI HE CHUNXIANG LIU CHUANFU CHENG | 2022 | Photonics Research2022，10，4： | 0 |
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