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| 1 | Development of a set of SSR markers for genetic polymorphism detection and interspecific hybrid jute breeding显示文摘Corchorus capsularis(white jute) and C. olitorius(dark jute) are the two principal cultivated spedes of jute that produce natural bast fiber of commercial importance. We have identified 4509 simple sequence repeat(SSR) loci from 34,163 unigene sequences of C. capsularis to develop a non-redundant set of 2079 flanking primer pairs. Among the SSRs, trinudeotide repeats were most frequent(60%) followed by dinucleotide repeats(37.6%). Annotation of the SSR-containing unigenes revealed their putative functions in various biological and molecular processes, including responses to biotic and abiotic signals. Eighteen expressed gene-derived SSR(eSSR) markers were successfully mapped to the existing single-nucleotide polymorphism(SNP) linkage map of jute,providing additional anchor points. Amplification of 72% of the 74 randomly selected primer pairs was successful in a panel of 24 jute accessions, comprising five and twelve accessions of C.capsularis and C. olitorius, respectively, and seven wild jute spedes. Forty-three primer pairs produced an average of 2.7 alleles and 58.1% polymorphism in a panel of 24 jute accessions. The mean PIC value was 0.34 but some markers showed PIC values higher than 0.5, suggesting that these markers can efficiently measure genetic diversity and serve for mapping of quantitative trait loci(QTLs) in jute. A primer polymorphism survey with parents of a wide-hybridized population between a cultivated jute and its wild relative revealed their efficacy for interspecific hybrid identificatioii. For ready accessibility of jute eSSR primers, we compiled all information in a user-friendly web database, JuteMarkerdb(http://gffzz18bcbf3a1ec04b9bh5ofqvnuqcbcf6qu5.ffgz.tsg.suse.edu.cn/) for the first time in jute.This eSSR resource in jute is expected to be of use in characterization of germplasm, interspecific hybrid and variety identification, and marker-assisted breeding of superior-quality jute. | Dipnarayan Saha Rajeev Singh Rana Sukla Chakraborty Subhojit Datta Arroju Anil Kumar Asim Kumar Chakraborty Pran Gobinda Karmakar | 2017 | The Crop Journal2017，5，5： | 3 |
| 2 | Clinical evaluation of xylazine with barbiturate and non - barbitturate anesthesia in dogs显示文摘 | Pathak J C Pandey S S Sukla B P | 1998 | Indian Veterinary Journal1998，75，10： | 2 |
| 3 | Study on surface alter ation behavior during column bioleaehing 显示文摘 | Mohapatra B Singh S Sukla L | 2012 | Miner Process Extr Metall Rev2012，33，6： | 1 |
| 4 | Insights into heap bioleaching of low grade chalcopyrite ores--A pilot scale study显示文摘 | PANDA S SAN JAY K SUKLA L B PRADHAN N SUBBAIAH T MISHRA B K PRASAD M S R RAY S K | 2012 | Hydrometallurgy2012，125，： | 1 |
| 5 | Studies on reaction mechanism of bioleaching of manganese ore显示文摘 | Acharya C Kar R N Sukla L B | 2003 | Minerals Engineering2003，16，10： | 1 |
| 6 | Bioleaehing of sukinda laterite using ultrasonics 显示文摘 | Sukla L B Swamy K M Narayana K L | 1995 | Hydrometallurgy1995，37，3： | 1 |
| 7 | Studies on Reaction Mech- anism of Bioleaching of Manganese Ore显示文摘 | Acharya C Kar R N Sukla L B | 2003 | Minerals Engi neering2003，16，10： | 1 |
| 8 | Nucleotide Sequence Analysis of the Capsid Genes and the Right-Hand Terminal Palindrome of Porcine Parvovirus, Strain NADL-2显示文摘 | Jayarama V Sukla B Ranga V S | 1989 | Viology1989，173，： | 1 |
| 9 | Bacterial removal of sulphur from three different coals显示文摘 | C Acharya R N Kar L B Sukla | 2001 | Fuel2001，80，： | 1 |
| 10 | Heap bioleaching of chalcopyrite: A review 显示文摘 | PARDHAN N NATHSARMA K C SRINNASA R K SUKLA L B MISHRA B K | 2008 | Minerals Engineering2008，21，5： | 1 |
| 11 | Bacterial removal of sulphur form three different coals显示文摘 | Acharya C Kar R N Sukla L B | 2001 | Fuel2001，80，15： | 1 |
| 12 | Studies on reactionmechanism of bioleaching of manganese ore 显示文摘 | ACHARYA C KAR R N SUKLA L B | 2003 | MineralsEngineering2003，16，10： | 1 |
| 13 | Bioleaching of lateritic nickel ore by ultrasound显示文摘 | R. N. Kar L. B. Sukla K. M. Swamy V. V. Panchanadikar K. L. Narayana | 1996 | Metallurgical and Materials Transactions B1996，，3： | 1 |
| 14 | Microbial extraction of nickel from Sukinda chromite overburden by Acidithiobacillus ferrooxidans and Aspergillus strains显示文摘 | MOHAPATRA S BOHIDHAR S PRADHAN N KAR R N SUKLA L B | 2007 | Hydrometallurgy2007，85，： | 1 |
| 15 | Studies on reaction mechanism of bioleaching of manganese ore显示文摘 | Aeharya C Kar R N Sukla L B | 2003 | Minerals Engineering2003，16，10： | 1 |
| 16 | Recovery of cobalt, nickel and copper from converter slag through roasting with ammonium sulphate and sulphuric acid 显示文摘 | Sukla L B Panda S C Jena P K | 1986 | Hydrometallurgy1986，16，2： | 1 |
| 17 | Bacterial removal of sulphur from three different coals显示文摘 | Acharya C Kar RN Sukla LB | 2001 | Fuel2001，80，： | 1 |
| 18 | Aqueous S02 leaching studies on Nishikhal manganese ore through factorial experiment 显示文摘 | Naik P K Sukla L B Das S C | 2000 | Hydrometallurgy2000，54，3： | 1 |
| 19 | Studies on reaction mechanism of bioleaching of manganese ore 显示文摘 | ACHARYA C KAR R N SUKLA L B | 2003 | Minerals Engineering2003，16，： | 1 |
| 20 | Degradation mechanism and control of blended eri and tasar silk nanofiber 显示文摘 | Panda N Biswas A Sukla LB | 2014 | Appl Biochem Biotechnol2014，17，41： | 1 |
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