|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Attenuation of MET-mediated migration and invasion in hepatocellular carcinoma cells by SOCS1显示文摘AIM To investigate the role of suppressor of cytokine signaling 1(SOCS1)in regulating MET-mediated invasive potential of hepatocellular carcinoma(HCC)cells.METHODSStable derivatives of mouse(Hepa1-6)and human(hep3B,Hep G2)HCC cell lines expressing SOCS1or control vector were evaluated for their ability to migrate towards hepatocyte growth factor(HGF)in the transwell migration assay,invade extracellular matrix in response to HGF stimulation in a 3-D invasion assay by confocal microscopy,and to undergo anchorageindependent proliferation in semisolid agar.Following intravenous and intrasplenic inoculation into NOD.scid.gamma mice,the ability of Hepa cells to form othotopic tumors was evaluated.Following HGF stimulation of Hepa and Hep3B cells,expression of proteins implicated in epithelial-to-mesenchymal transition was evaluated by western blot and qR T-PCR.RESULTS SOCS1 expression in mouse and human HCC cells inhibited HGF-induced migration through matrigel.In the 3-D invasion assay,HGF stimulation induced invasion of HCC cells across type-Ⅰcollagen matrix,and SOCS1expression significantly reduced the depth of invasion.SOCS1 expression also reduced the number and size of colonies formed by anchorage-independent growth in semisolid agar.Following intravenous inoculation,control Hepa cell formed large tumor nodules that obliterated the liver whereas the SOCS1-expressing Hepa cells formed significantly smaller nodules.Tumors formed by SOCS1-expressing cells showed reduced phosphorylation of STAT3 and ERK that was accompanied by reduced levels of MET protein expression.HGF stimulated Hepa cells expressing SOCS1 showed increased expression of E-cadherin and decreased expression of EGR1,SNAI1and ZEB1.Comparable results were obtained with Hep3B cells.SOCS1 expressing HCC cells also showed reduced levels of EGR1 and SNAI1 transcripts.CONCLUSION Our findings indicate that loss of SOCS1-dependent control over epithelial-to-mesenchymal transition may contribute to MET-mediated migration,invasion and metastatic growth of HCC. | Yirui Gui Md Gulam Musawwir Khan Diwakar Bobbala Claire Dubois Sheela Ramanathan Caroline Saucier Subburaj Ilangumaran | 2017 | World Journal of Gastroenterology2017，23，36： | 3 |
| 2 | Molecular characterization and dynamic expression patterns of two types of γ-gliadin genes from Aegilops and Triticum species显示文摘 | Shunli Wang Xixi Shen Pei Ge Jie Li Saminathan Subburaj Xiaohui Li F. J. Zeller S. L. K. Hsam Yueming Yan | 2012 | Theoretical and Applied Genetics2012，，7： | 2 |
| 3 | Trans-presentation of interleukin-15 by interleukin-15 receptor alpha is dispensable for the pathogenesis of autoimmune type 1 diabetes显示文摘Interleukin-15(IL-15)is a pro-inflammatory cytokine that is required for the survival and activation of memory CD8^(+)T cells,natural killer(NK)cells,innate lymphoid cells,macrophages and dendritic cells.IL-15 is implicated in the pathogenesis of various autoimmune diseases such as rheumatoid arthritis,inflammatory bowel disease,psoriasis and autoimmune type 1 diabetes(T1D).IL-15 receptor(IL-15R)consists of a specificαchain,theβchain that is shared with IL-2R and the commonγchain.IL-15 is unique in the manner in which it binds and signals through its receptor subunits.IL-15 that is complexed with IL-15Rαbinds to theβγreceptor complex present on the responding cell to mediate its biological effects through a process referred to as trans-presentation.The trans-presented IL-15 is essential to mediate the biological effects on T lymphocytes and NK cells.Here we show that IL-15,but not IL-15Rα,is required for the development of spontaneous and virus-induced T1D,viral clearance and for antigen cross-presentation to CD8^(+)T lymphocytes.Our findings provide insight into the complexities of IL-15 signalling in the initiation and maintenance of CD8^(+)T cell-mediated immune responses. | Diwakar Bobbala Marian Mayhue Alfredo Menendez Subburaj Ilangumaran Sheela Ramanathan | 2017 | Cellular & Molecular Immunology2017，14，7： | 2 |
| 4 | Non linear support vector machine based partial discharge patterns recognition using fractal features显示文摘 | MASHESWARI R V SUBBURAJ R VIGNESHWARAN B | 2014 | Journal of Intelligent and Fuzzy Systems2014，27，5： | 1 |
| 5 | Optimization of elec- trophoretic suspensionto fabricate Li O2 based posi- tive electrode for Li-ion batteries 显示文摘 | PRASANNA K SUBBURAJ T JOY N | 2013 | Eleetroehimica Acta2013，95，： | 1 |
| 6 | Automated analysis of giant unilamellar vesicles using circular Hough transformation显示文摘 | Hermann E Bleicken S Subburaj Y | 2014 | Bioinformatics2014，30，12： | 1 |
| 7 | Speed control of switched reluctance motor with torque ripple reduction using non-dominated sorting genetic algorithm (NSGA-Ⅱ)显示文摘 | KALAIVANI L SUBBURAJ P IRUTHAYA-RAJAN M W | 2013 | Electrical Power and Energy Systems2013，，53： | 1 |
| 8 | Direct model reference adaptive internal model controller for better voltage sag ride through in doubly fed induction generator wind farms显示文摘 | AMUTHAN N SUBBURAJ P MARY P M | 2013 | Electrical Power and Energy Systems2013，47，： | 1 |
| 9 | Methods of Recording and Analysing Cough Sounds显示文摘 | Subburaj S Parvez L Rajagopalan TG | 1996 | Pulm Pharmacol1996，9，： | 1 |
| 10 | Speed control of switched reluctance motor with torque tipple reduction u- sing non-dominated sorting genetic algorithm (NSGA-II) 显示文摘 | Kalaivani L Subburaj P Iruthayarajan M W | 2013 | International Journal of Electrical Power & Energy Sys- tems2013，53，： | 1 |
| 11 | Rapid development of auricular prosthesis using CAD and rapid prototyping technologies显示文摘 | Subburaj K Nair C Rajesh S | 2007 | Int J O- ral Maxillofae Surg2007，36，10： | 1 |
| 12 | Application and validation of a computerized cough acquisition system for objective monitoring of acutecough: a meta anylysis 显示文摘 | Pavesi L Subburaj S Porter-Shaw K | 2001 | Chest2001，120，： | 1 |
| 13 | Rapid development of auricular prosthesis using CAD and rapid prototyping technologies显示文摘 | Subburaj K Nair C Rajesh S | 2007 | International Journal of Oral and Maxillofacial Surgery2007，36，10： | 1 |
| 14 | Rapid development of auricular prosthesis using CAD and rapid prototyping technologies显示文摘 | SUBBURAJ K NAIR C RAJESH S | 2007 | Int J Oral Maxillofac Surg2007，36，： | 1 |
| 15 | Mandibular reconstruction through transport distraction using intraoral appliance显示文摘 | Andrade NN Reshamwala SM Subburaj K | 2011 | J Oral Maxillofac Surg2011，69，8： | 1 |
| 16 | Distribution systemreconfiguration for loss reduction using genetic algorithm 显示文摘 | SUBBURAJ P RAMAR K GANESAN L | 2006 | Journal of Electrical Systems2006，2，4： | 1 |
| 17 | Rapid development of auricu- lar prosthesis using CAD and rapid prototyping technologies 显示文摘 | Subburaj K Nalr C Rajesh S | 2007 | Oral Maxillofac Surg2007，36，10： | 1 |
| 18 | Non linear support vector machine based partial discharge patterns recognition using fractal features显示文摘 | Maheswari R V Subburaj P Vigneshwaran B | 2014 | Journal of Intelligent & Fuzzy Systems2014，27，5： | 1 |
| 19 | Rapid development of auricular prosthesis using CAD and rapid prototyping technologies显示文摘 | Subburaj K Nair C Rajesh S | 2007 | Int J Oral Maxillofac Surg2007，36，10： | 1 |
| 20 | Application and validation of a computerized cough acquisition system for objective monitoring of acute cough: a meta-analysis 显示文摘 | Pavesi L Subburaj S Porter-Shaw K | 2001 | Chest2001，120，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员