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| 1 | Cloning and expression of the first gene for biodegrading microcystin LR by Sphingopyxis sp.USTB-05显示文摘Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters,the biodegradation is found to be the most efficient method for removing microcystins(MCs) produced by harmful cyanobacteria.Based on the isolation of a promising bacterial strain of Sphingopyxis sp.USTB-05 for biodegrading MCs,we for the first time cloned and expressed a gene USTB-05-A(HM245411) that is responsible for the first step in the biodegradation of microcystin LR(MC-LR) in E.coli DH5α,with a cloning vector of pGEM-T easy and an expression vector of pGEX-4T-1,respectively.The cell-free extracts(CE) of recombinant E.coli DH5α containing USTB-05-A had high activity for biodegrading MC-LR.The initial MC-LR concentration of 40 mg/L was completely biodegraded within 1 hr in the presence of CE with a protein concentration of 0.35 mg/mL.Based on an analysis of the liquid chromatogram-mass spectrum(LC-MS),the enzyme encoded by gene USTB-05-A was found to be active in cleaving the target peptide bond between 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyl-deca-4,6-dienoic acid(Adda) and arginine of MC-LR,and converting cyclic MC-LR to linear MC-LR as a first product that is much less toxic than parent MC-LR,which offered direct evidence for the first step on the pathway of MC-LR biodegradation by Sphingopyxis sp.USTB-05. | Hai Yan Huasheng Wang Junfeng Wang Chunhua Yin Song Max Xiaolu Liu Xueyao Yin | 2012 | Journal of Environmental Sciences2012，24，10： | 6 |
| 2 | Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomallevel genome analysis of Akebia trifoliata subsp . australis显示文摘Akebia trifoliata subsp.australis is a well-known medicinal and potential woody oil plant in China.The limited genetic information available for A.trifoliata subsp.australis has hindered its exploitation.Here,a high-quality chromosomelevel genome sequence of A.trifoliata subsp.australis is reported.The de novo genome assembly of 682.14 Mb was generated with a scaffold N50 of 43.11 Mb.The genome includes 25,598 protein-coding genes,and 71.18%(485.55 Mb)of the assembled sequences were identi fi ed as repetitive sequences.An ongoing massive burst of long terminal repeat(LTR)insertions,which occurred~1.0 million years ago,has contributed a large proportion of LTRs in the genome of A.trifoliata subsp.australis.Phylogenetic analysis shows that A.trifoliata subsp.australis is closely related to Aquilegia coerulea and forms a clade with Papaver somniferum and Nelumbo nucifera,which supports the well-established hypothesis of a close relationship between basal eudicot species.The expansion of UDP-glucoronosyl and UDP-glucosyl transferase gene families and fi-amyrin synthase-like genes and the exclusive contraction of terpene synthase gene families may be responsible for the abundant oleanane-type triterpenoids in A.trifoliata subsp.australis.Furthermore,the acyl-ACP desaturase gene family,including 12 stearoyl-acyl-carrier protein desaturase(SAD)genes,has expanded exclusively.A combined transcriptome and fatty-acid analysis of seeds at fi ve developmental stages revealed that homologs of SADs,acyl-lipid desaturase omega fatty acid desaturases(FADs),and oleosins were highly expressed,consistent with the rapid increase in the content of fatty acids,especially unsaturated fatty acids.The genomic sequences of A.trifoliata subsp.australis will be a valuable resource for comparative genomic analyses and molecular breeding. | Hui Huang Juan Liang Qi Tan Linfeng Ou Xiaolin Li Caihong Zhong Huilin Huang Ian Max Moller Xianjin Wu Songquan Song | 2021 | Horticulture Research2021，8，1： | 5 |
| 3 | Prevalence and trend of major transfusion-transmissible infections among blood donors in western China,2005 through 2010显示文摘 | Yan Song Ying Bian Max Petzold | 2014 | PLoS One2014，9，94： | 1 |
| 4 | 显示文摘 | Zhao X S Lu Max G Q Song C | 2003 | J Mol Catal A:Chem2003，191，： | 1 |
| 5 | A new approach to deep desulfurization of gasoline, diesel fuel and jet fuel by selective adsorption for ultra-clean fuels and for fuel cell applications显示文摘 | MAX L SUN L SONG C S | 2002 | Catal Today2002，77，12： | 1 |
| 6 | A new approach to deepdesulfur ization of gasoline, diesel fuel and jet fuel by selective adsorp tion for ultra-clean fuels and for cell applications显示文摘 | MAX L SUN L SONG C S | 2002 | Catalysis Today2002，77，： | 1 |
| 7 | Dynamic wheeled motion control of wheel-biped transformable robots显示文摘Most existing biped robots can only walk with their feet or move by wheels.To combine the best of both worlds,this paper introduces the dynamic wheeled control including wheeled locomotion and in-situ wheel-to-foot(WtF)transformation of a full-sized wheel-biped transformable robot SR600-II.It can traverse on flat surfaces by wheels and transform to footed stance through its switching modules when facing obstacles.For wheeled locomotion,the kinematics considering upper-body lumped center-of-mass(CoM)constraint is first derived.Then,the dynamics of wheeled locomotion is modeled as a wheeled inverted pendulum(WIP)with variables related to the pose of upper body.After that,a parameter-varying linear quadratic regulator(LQR)controller is utilized to enable dynamic wheeled locomotion.For WtF transformation,the WtF balance constraints are first revealed.Then,a WtF transformation strategy is proposed to tackle the problem when robot transforms from wheeled balance state to in-situ biped stance state.It enables the robot to pass by the transition stages in which both wheels and feet touch the ground and to maintain its balance at the same time.Simulations and experiments on the SR600-II prototype have validated the efficacy of proposed dynamic wheeled control strategies for both wheeled locomotion and in-situ WtF transformation. | Chao Zhang Tangyou Liu Shuang Song Jiaole Wang Max Q-H.Meng | 2022 | Biomimetic Intelligence & Robotics2022，2，2： | 1 |
| 8 | Analysis and comparison of nitrogen compounds in different liquid hydrocarbon streams derived from petroleum and coal显示文摘 | LI N MAX L ZHA Q F SONG C S | | 0，，10： | 1 |
| 9 | Associations o{ the ATP-binding cassette transporter A1 R219K polymor- phism with HDL-C level and coronary artery disease risk: A meta-analysis 显示文摘 | MAX Y LIU J P SONG Z Y | 2011 | Atherosclerosis2011，215，： | 1 |
| 10 | Influence of nitrogen compounds on deep hydrodesulfurization for 4, 6 AI203- and MCM-41-supported Co-Mo sulfide catalysts显示文摘 | TURAGA U T MAX L SONG C S | 2003 | Catal Today2003，86，14： | 1 |
| 11 | Synthesis and application of starch-graft-poly (AM-co-AMPS) by using a complex initiation system of CS-APS显示文摘 | SONG H ZHANG S F MAX C | 2007 | Carbohydrate Polymers2007，69，1： | 1 |
| 12 | Structure and tribological performance of helium-implanted layer on Ti6A14V alloy by plasma-based ion implantation 显示文摘 | LI J L MAX X SUN M G SONG Z L | 2009 | Nuclear Instruments and Methods in Physics Research B2009，267，： | 1 |
| 13 | Cardiopulmonary resuscitation in the mouse 显示文摘 | Lei Song Max Harry Weil Wanchun Tang | 2002 | Journal of Applied Physiology2002，93，4： | 1 |
| 14 | Selective adsorption for removing sulfur from jet fuel over zeolite-based adsorbents 显示文摘 | VELU S MAX L SONG C S | 2003 | Industrial & Engineering Chemistry Research2003，42，21： | 1 |
| 15 | A new approach to deep desulfurization of gasoline, diesel fuel and jet fuel by selective adsorption for ultra-clean fuels and for fuel cell applications显示文摘 | MAX L SUN L SONG C S | 2002 | Catalysis Today2002，77，： | 1 |
| 16 | A New approach to deep desulfurization of gasoline, diesel fuel and jet fuel by se- lective adsorption for ultra-clean fuels and for fuel cell ap- plications 显示文摘 | MAX L SUN L SONG C S | 2002 | Catal Today2002，77，12： | 1 |
| 17 | New design approaches to ultra-clean diesel fuels by deep desulfurization and deep dearomatization 显示文摘 | SONG C S MAX L | 2003 | Appl Catal B: Environ2003，41，12： | 1 |
| 18 | New design approaches to ultra-clean diesel fuels by deep desulfurigation and deep dearomatigation显示文摘 | SONG C S MAX | 2003 | Appl Catal B2003，41，12： | 1 |
| 19 | Deep desulfurization of gasoline by selective adsorption over nickel-based adsorbent for fuel cell applications显示文摘 | MAX L SPRAGUE M SONG C S | 2005 | Ind Eng Chem Res2005，44，15： | 1 |
| 20 | Deep desulfurization of gasoline by selective adsorption over solid adsorbents and impact of analytical methods on ppm-level sulfur quantification for fuel cell applications 显示文摘 | MAX L KIM J H SONG C S | 2005 | Appl Catal B2005，56，12： | 1 |
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